FastQCFastQC Report
Wed 25 May 2016
SRR1780687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554413
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT125052.2555387409746883No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT40660.7333882863497068RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC24120.43505473356504987No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC12670.22852999478727953No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG10760.19407914316583486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTAC357.2718176E-637.7230223
AGAGCGT250.00234902635.20815312
CCGTCTT4750.034.73235744
ATTGGCG1950.031.58566721
GGTGATC353.2112663E-431.43585412
GCCGTCT5250.031.42451343
CGCCATT2000.030.79602417
ATGCCGT19400.029.70750444
TAGGTAC300.005735866729.340133
TATGCCG20100.028.78235243
TTGGCGT2150.027.6243422
CGCCAAT406.969803E-427.5386711
TCGAGTC655.928141E-727.07342533
GCGTGAA2200.026.99651525
GAGGCGC2300.026.78881513
GTATCGA508.3305276E-526.40135216
TGGCGTG2250.026.39659123
CGATAAC508.340758E-526.39659143
AAGGTAG2350.026.20938130
TGCCGTC6350.025.98089642
Produced by FastQC (version 0.11.5)