FastQCFastQC Report
Wed 25 May 2016
SRR1780686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37101
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCAC252.362024E-455.99460656
TATACAA252.362024E-455.9946062
ATATACA200.006719549752.8510861
CTGTAGT200.006902019552.49494663
CACGGTA200.006902019552.49494638
AGTCACC200.006902019552.4949468
CCGGCTT200.006902019552.49494632
ATCCGAC200.006902019552.49494611
TAATTCC200.006902019552.4949464
CGGTACC200.006902019552.49494640
ACGGTAC200.006902019552.49494639
ACCTGTA350.001240717239.9961561
TCCTACG350.001240717239.996158
ACGAACG1253.3323886E-927.99730370
GACGAAC1604.6009518E-821.87289469
CGACGAA1801.596527E-719.44257468
CCGACGA2108.055067E-716.66506267
GCCGACG2504.946829E-613.998651566
TGCCGAC2751.3235562E-512.72604765
CTGCCGA3206.245022E-510.93644764
Produced by FastQC (version 0.11.5)