##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780686_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37101 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.682057087410044 34.0 24.0 34.0 12.0 34.0 2 30.18093851917738 34.0 31.0 34.0 21.0 34.0 3 30.58998409746368 34.0 32.0 34.0 21.0 34.0 4 30.893668634268618 34.0 32.0 34.0 21.0 34.0 5 30.50521549284386 34.0 31.0 34.0 21.0 34.0 6 33.329101641465186 37.0 34.0 38.0 21.0 38.0 7 32.421093771057386 37.0 31.0 38.0 11.0 38.0 8 31.245411174900948 36.0 27.0 38.0 11.0 38.0 9 32.244413897199536 37.0 31.0 38.0 12.0 38.0 10-11 32.59425621950891 37.0 31.5 38.0 16.0 38.0 12-13 33.07944529797041 37.0 33.0 38.0 21.0 38.0 14-15 32.642920675992556 37.0 31.5 38.0 11.0 38.0 16-17 32.5416565591224 37.0 31.0 38.0 11.5 38.0 18-19 32.544702299129405 37.0 32.0 38.0 11.0 38.0 20-21 32.614053529554454 37.0 31.0 38.0 11.0 38.0 22-23 32.58370394328993 37.0 31.5 38.0 11.0 38.0 24-25 33.09669550685966 37.0 33.5 38.0 21.0 38.0 26-27 33.18376863157327 37.0 34.0 38.0 21.0 38.0 28-29 33.28264736799547 37.0 34.0 38.0 21.0 38.0 30-31 33.10099458235627 37.0 34.0 38.0 21.0 38.0 32-33 32.89864154604997 37.0 33.5 38.0 16.0 38.0 34-35 32.742486725425195 37.0 32.5 38.0 11.0 38.0 36-37 32.657421632840084 37.0 32.0 38.0 11.0 38.0 38-39 33.239670089754995 37.0 34.0 38.0 21.0 38.0 40-41 32.71759521306703 37.0 32.5 38.0 11.0 38.0 42-43 33.082895339748255 37.0 34.0 38.0 21.0 38.0 44-45 32.056575294466455 37.0 31.0 38.0 11.0 38.0 46-47 32.763699091668684 37.0 32.5 38.0 11.0 38.0 48-49 32.49278995175332 37.0 31.0 38.0 11.0 38.0 50-51 32.461429611061696 37.0 31.5 38.0 11.0 38.0 52-53 32.426619228592216 37.0 31.0 38.0 11.0 38.0 54-55 32.09173607180399 37.0 31.0 38.0 11.0 38.0 56-57 32.20911835260505 37.0 31.0 38.0 11.0 38.0 58-59 32.06238376324088 37.0 29.0 38.0 11.0 38.0 60-61 32.077666370178704 37.0 31.0 38.0 11.0 38.0 62-63 32.16049432629848 37.0 31.0 38.0 11.0 38.0 64-65 32.1888224037088 37.0 31.0 38.0 11.0 38.0 66-67 32.173014204468885 37.0 31.0 38.0 11.0 38.0 68-69 31.6722056009272 37.0 29.0 38.0 11.0 38.0 70-71 31.572545214414706 36.5 28.0 38.0 11.0 38.0 72-73 31.62120967089836 37.0 28.5 38.0 11.0 38.0 74-75 31.195331662219346 36.0 27.5 38.0 11.0 38.0 76 30.423977790356055 35.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1974769884751204 1101 2 -1.2770539782979817 1101 3 -0.0617871100192211 1101 4 -2.7094864213899967 1101 5 -0.5897032312252577 1101 6 -3.125440323621259 1101 7 -0.8028593748402102 1101 8 -1.3413063424394984 1101 9 -0.8259452545632477 1101 10-11 -1.2658849663205487 1101 12-13 -0.5400118209647218 1101 14-15 0.12787428372973864 1101 16-17 -2.442502256954864 1101 18-19 0.42868902577330914 1101 20-21 -0.8184970719467692 1101 22-23 0.6229278265174543 1101 24-25 -0.20038959084314456 1101 26-27 -0.09836908020302104 1101 28-29 0.5792595990333496 1101 30-31 -0.3006532926709298 1101 32-33 0.9322658396838861 1101 34-35 -0.6391249552229112 1101 36-37 0.8373053018492271 1101 38-39 -0.4288889249952277 1101 40-41 0.13713600949575522 1101 42-43 -0.2857422286062814 1101 44-45 0.458199876443075 1101 46-47 1.0834731520968788 1101 48-49 -0.880913004098872 1101 50-51 -0.6541974163541724 1101 52-53 0.2474972303753944 1101 54-55 -0.4588064383706829 1101 56-57 -0.1810397438132867 1101 58-59 0.1705432430097602 1101 60-61 0.7151327976905506 1101 62-63 -2.0810518188691702 1101 64-65 0.4554861382320041 1101 66-67 -1.0585800277163848 1101 68-69 0.9495825358209835 1101 70-71 0.10095688866109143 1101 72-73 -1.0571926439614217 1101 74-75 -1.1550691101299861 1101 76 -3.4558417348808135 1102 1 1.5000020031215158 1102 2 -0.3446410131119073 1102 3 -0.15554461302042455 1102 4 0.6815099771694264 1102 5 1.7397085334806235 1102 6 1.7451479116728592 1102 7 2.2399977680169343 1102 8 3.3799421569602615 1102 9 1.075615369686453 1102 10-11 -0.028670080366168804 1102 12-13 -3.3158821691039826 1102 14-15 -0.40759990594612816 1102 16-17 -3.1382405522729897 1102 18-19 -0.8004826428941527 1102 20-21 0.13492429660065142 1102 22-23 -0.07749234154977103 1102 24-25 0.2916872398891428 1102 26-27 -5.569137387525949 1102 28-29 0.5980471140393462 1102 30-31 1.1364415452642476 1102 32-33 -6.0894628517927 1102 34-35 0.912015500959555 1102 36-37 0.9574733690761192 1102 38-39 0.5917593343084917 1102 40-41 1.1451792267826661 1102 42-43 1.3413285997250455 1102 44-45 -4.794861348046723 1102 46-47 0.1858741124810308 1102 48-49 0.7262898770535884 1102 50-51 -4.832468724877582 1102 52-53 -1.556464354258459 1102 54-55 1.1272079673916267 1102 56-57 -0.5373422648216888 1102 58-59 1.6927521265631782 1102 60-61 1.2600907808838215 1102 62-63 -3.746658061366169 1102 64-65 0.023073173045929707 1102 66-67 0.7471222531959754 1102 68-69 -6.547656359737239 1102 70-71 0.2462750159119942 1102 72-73 0.9617149190637946 1102 74-75 -5.435321210970322 1102 76 -1.646718085421032 1103 1 -0.2077306312166236 1103 2 -0.473923918934787 1103 3 -0.8257374608017365 1103 4 0.15192750197751437 1103 5 0.26264431329713744 1103 6 -2.7869621800702618 1103 7 0.21181952424235817 1103 8 0.15507789933060323 1103 9 0.5124249559062619 1103 10-11 1.2471209699989316 1103 12-13 -1.2876103099015879 1103 14-15 -0.42112733526648327 1103 16-17 -1.11319842533047 1103 18-19 0.20532151363083528 1103 20-21 -1.2433756472300956 1103 22-23 -0.5142551200556724 1103 24-25 0.6735445699771461 1103 26-27 -0.41752720216740613 1103 28-29 0.7540572245055586 1103 30-31 0.8496974898439049 1103 32-33 -4.637674797121292 1103 34-35 -0.27830466380359553 1103 36-37 0.12850875570783415 1103 38-39 0.5083479881186435 1103 40-41 0.770811130920471 1103 42-43 1.4407013763586392 1103 44-45 -4.6051028758505055 1103 46-47 0.23932852176768193 1103 48-49 0.6251852150052812 1103 50-51 0.9726239543959636 1103 52-53 0.4991432098681159 1103 54-55 0.6973727305445863 1103 56-57 1.4364454153880146 1103 58-59 0.9635815592536829 1103 60-61 1.3377447782625893 1103 62-63 0.9379983700137231 1103 64-65 0.7853836018045826 1103 66-67 0.11891592479305757 1103 68-69 -2.3845782844704324 1103 70-71 1.0528187343167836 1103 72-73 1.3224619701778124 1103 74-75 -0.41205777715643066 1103 76 0.39035362399668116 1104 1 -1.8307573276378157 1104 2 0.6306301764449955 1104 3 1.2141005423390183 1104 4 0.47641712921943835 1104 5 0.2442130379851264 1104 6 1.1865893531143001 1104 7 2.4169604449796083 1104 8 1.38270001686098 1104 9 0.24347710897676222 1104 10-11 1.0718998773466524 1104 12-13 1.3962891859245161 1104 14-15 0.48865859745522755 1104 16-17 0.4821400323933105 1104 18-19 1.893394908126254 1104 20-21 2.270427146389217 1104 22-23 1.4492129351555079 1104 24-25 1.366701029188647 1104 26-27 2.214508503262799 1104 28-29 0.5403618777826864 1104 30-31 -0.12794529055116755 1104 32-33 0.6236277898693672 1104 34-35 0.12685278722541682 1104 36-37 1.2116561232588765 1104 38-39 0.5445080013428765 1104 40-41 2.318005113894266 1104 42-43 1.596292747546343 1104 44-45 -1.389547871304675 1104 46-47 1.7958207938562794 1104 48-49 1.9795533446027633 1104 50-51 2.2652514442711578 1104 52-53 1.044105603454362 1104 54-55 0.6074892691014924 1104 56-57 1.743867516388093 1104 58-59 1.8584075779329154 1104 60-61 1.6143378851309294 1104 62-63 1.9598596808658613 1104 64-65 1.0387929744800175 1104 66-67 1.463200576417158 1104 68-69 -3.3022987572367803 1104 70-71 -0.2730759677247043 1104 72-73 1.5527702601191322 1104 74-75 -0.48326200891112236 1104 76 0.5764847092103373 1105 1 -0.40733390421439 1105 2 -0.04504549923067813 1105 3 -0.0922057639672893 1105 4 0.6295702823725904 1105 5 1.0550238487959405 1105 6 0.8442470107719586 1105 7 2.065609093628254 1105 8 2.1034207375817005 1105 9 1.2074410729407248 1105 10-11 2.6709989764457447 1105 12-13 -0.8874945978592734 1105 14-15 0.6598297686264019 1105 16-17 1.946103996357273 1105 18-19 1.7717732865046258 1105 20-21 1.3560127319747934 1105 22-23 1.4086723946149604 1105 24-25 1.5829172454048717 1105 26-27 2.2415355302898234 1105 28-29 0.3691907066115192 1105 30-31 1.160342997737125 1105 32-33 1.767771934013517 1105 34-35 1.243969904342535 1105 36-37 1.8287732403759946 1105 38-39 1.265228722063597 1105 40-41 1.7008879967771477 1105 42-43 1.1278242790778705 1105 44-45 2.155497173740372 1105 46-47 2.151676649712144 1105 48-49 1.0291028941523166 1105 50-51 1.5084946875143999 1105 52-53 0.5756371349858895 1105 54-55 0.43181359342581516 1105 56-57 1.0276513001718754 1105 58-59 0.11966883919417626 1105 60-61 0.7855090563020966 1105 62-63 1.1760758970820788 1105 64-65 1.6649191006061415 1105 66-67 1.8100474232640025 1105 68-69 2.2022057472677297 1105 70-71 2.1863834917347553 1105 72-73 1.435653143002014 1105 74-75 -0.9832620089111224 1105 76 -1.594686461960837 1106 1 0.08026709885053052 1106 2 -0.24333657381041363 1106 3 -1.2023571524691938 1106 4 -0.19972292836391858 1106 5 0.46135801801670695 1106 6 -0.502274768739511 1106 7 0.4528107282525724 1106 8 0.2623322263900931 1106 9 1.276541100803641 1106 10-11 0.7697730082094694 1106 12-13 0.5682962445295132 1106 14-15 -0.08522456405679435 1106 16-17 0.8816942257619331 1106 18-19 0.06865264008501981 1106 20-21 -0.06494946733791807 1106 22-23 0.5922425627212178 1106 24-25 0.5019754788960853 1106 26-27 0.28416578941492787 1106 28-29 -1.7993334876858746 1106 30-31 -0.11308506950537378 1106 32-33 0.5527631107572688 1106 34-35 0.3883462394405228 1106 36-37 0.3101979640687489 1106 38-39 0.8889400622697821 1106 40-41 1.1065720313271683 1106 42-43 0.4961255700595757 1106 44-45 2.007684412525549 1106 46-47 0.8205259113449017 1106 48-49 0.8570493973565547 1106 50-51 -1.0468596358811624 1106 52-53 0.4687828580285256 1106 54-55 0.1804901049889338 1106 56-57 0.526722536356516 1106 58-59 -0.11326512569854685 1106 60-61 0.3304147867748384 1106 62-63 0.7157341119980245 1106 64-65 0.4320780120949479 1106 66-67 0.9303223373533527 1106 68-69 1.0814200130799314 1106 70-71 0.7646318431789858 1106 72-73 0.8854998969724761 1106 74-75 -1.0881194436654624 1106 76 -0.011329909532118876 1107 1 0.5267877174072311 1107 2 0.17704266035747906 1107 3 0.706700284938762 1107 4 0.9646732424755484 1107 5 -0.09957718080509181 1107 6 1.4326479116728592 1107 7 1.525712053731219 1107 8 1.9845339936949529 1107 9 1.0590337370333884 1107 10-11 1.0312788992256685 1107 12-13 1.101847422732746 1107 14-15 0.48717050221713265 1107 16-17 1.2227288354821155 1107 18-19 1.9692877652691152 1107 20-21 2.2816079700700342 1107 22-23 1.029650515593083 1107 24-25 0.5053351990728174 1107 26-27 0.33456159206588154 1107 28-29 -0.7712130900422807 1107 30-31 0.7199874636315897 1107 32-33 2.025333066574646 1107 34-35 2.0963267254493587 1107 36-37 0.39497336907611924 1107 38-39 0.9304072934921663 1107 40-41 1.479362900252056 1107 42-43 1.4376296201332082 1107 44-45 1.1126641621573583 1107 46-47 1.2764353369708274 1107 48-49 1.6720806933801242 1107 50-51 1.6777353567550648 1107 52-53 1.5347346253333782 1107 54-55 1.8650906204528468 1107 56-57 2.025157735178304 1107 58-59 1.800532738808073 1107 60-61 1.1172336380266827 1107 62-63 1.0486225508787328 1107 64-65 1.8362109281479633 1107 66-67 2.190999804216389 1107 68-69 2.762292619854602 1107 70-71 1.0817341995854655 1107 72-73 1.9036792047780722 1107 74-75 0.8637859318868202 1107 76 1.1849145676401953 1108 1 -1.173643317075527 1108 2 0.38055251257422995 1108 3 -0.31300414855877534 1108 4 -0.6320016343717398 1108 5 0.4379843955495879 1108 6 -0.4445072607409344 1108 7 0.7523130389528916 1108 8 0.8312088705422482 1108 9 0.016853934077722954 1108 10-11 0.47924687952124145 1108 12-13 0.030880673964276184 1108 14-15 0.2831988273402928 1108 16-17 -1.2486381595917777 1108 18-19 -0.4212602642875396 1108 20-21 0.16492077795180649 1108 22-23 -0.8435021937665184 1108 24-25 -1.4985133230946666 1108 26-27 -0.934373136998154 1108 28-29 -0.5586207255102664 1108 30-31 0.17673007446903455 1108 32-33 0.13186016017071012 1108 34-35 -0.5205144075555665 1108 36-37 -0.2438813107268345 1108 38-39 0.35143561861532646 1108 40-41 -0.31681936575780156 1108 42-43 -0.4713913158273755 1108 44-45 0.12113091092583517 1108 46-47 0.6246496226851121 1108 48-49 -0.19876290267899321 1108 50-51 0.06689791833142067 1108 52-53 -0.3675129116124296 1108 54-55 0.25625441355629164 1108 56-57 0.6535444346856991 1108 58-59 -1.4813330247387206 1108 60-61 1.0421104853173233 1108 62-63 -0.9564574983823526 1108 64-65 0.2547761990839277 1108 66-67 -0.6482859100693261 1108 68-69 0.9319354769974559 1108 70-71 1.4268696675657573 1108 72-73 0.6907789584726629 1108 74-75 0.855934946665144 1108 76 0.7551116119751704 1109 1 -1.907207609695572 1109 2 -0.3580875132072734 1109 3 1.1796642368746753 1109 4 0.18913919708169047 1109 5 0.9359702157236143 1109 6 0.7544937060653822 1109 7 -1.23463507577479 1109 8 0.7507222446962913 1109 9 0.3147914139359287 1109 10-11 1.5316961221896221 1109 12-13 -0.11857146912305438 1109 14-15 0.15376763172314156 1109 16-17 -1.2307925263336443 1109 18-19 0.5709650015841987 1109 20-21 0.38249248275361225 1109 22-23 0.46289484136077164 1109 24-25 0.8142888038792364 1109 26-27 0.7912955707040652 1109 28-29 -0.285941060669785 1109 30-31 0.6769717760481484 1109 32-33 1.0912542948790573 1109 34-35 0.9275600365307923 1109 36-37 0.13103812208012755 1109 38-39 1.2231726472972468 1109 40-41 0.0396098962467164 1109 42-43 0.237237430547097 1109 44-45 -0.15114591794944943 1109 46-47 0.11997672548885419 1109 48-49 0.4631771553293831 1109 50-51 1.2064402966749697 1109 52-53 0.9898831567085509 1109 54-55 0.6971583774621948 1109 56-57 0.5474374414533472 1109 58-59 0.5353041006238328 1109 60-61 1.5367930505767475 1109 62-63 0.4573758886624404 1109 64-65 0.47577200958988897 1109 66-67 0.4206393235755357 1109 68-69 1.1095055704554007 1109 70-71 0.1806160420420717 1109 72-73 0.12092720210785046 1109 74-75 2.4842382015797426 1109 76 0.1879185729806494 1110 1 1.6142877174072296 1110 2 0.637919283734103 1110 3 0.5034535316920064 1110 4 0.9429199957223027 1110 5 0.9033448971169875 1110 6 1.2996933662183139 1110 7 1.0607769887961496 1110 8 3.2517417859027447 1110 9 0.30400126950092243 1110 10-11 -0.7912860358392635 1110 12-13 0.7688117084470392 1110 14-15 0.7310828398794698 1110 16-17 1.0099041601574328 1110 18-19 0.7979403626717101 1110 20-21 -0.03186605590398983 1110 22-23 0.826403762346331 1110 24-25 0.4982735107611376 1110 26-27 -0.40318191442761986 1110 28-29 0.8803291177499233 1110 30-31 0.17234135973548348 1110 32-33 1.0646187808603642 1110 34-35 1.376115686488319 1110 36-37 0.1977331093358572 1110 38-39 0.9243196311545034 1110 40-41 1.2582590041481652 1110 42-43 1.1078406590942507 1110 44-45 1.9900180582612492 1110 46-47 0.22919507723056398 1110 48-49 0.6517073167567418 1110 50-51 0.0861769151966314 1110 52-53 0.9536469629957196 1110 54-55 1.0588406204528482 1110 56-57 0.5847356572562319 1110 58-59 -2.8690289495036083 1110 60-61 0.31593493672797734 1110 62-63 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58-59 -0.6116696421443066 2116 60-61 -1.8113377905447479 2116 62-63 0.919158265164441 2116 64-65 -0.04325335756631432 2116 66-67 -0.6066192434026618 2116 68-69 -0.020445475383496614 2116 70-71 -2.974813419462155 2116 72-73 -0.2823326999838258 2116 74-75 -2.0365116871608 2116 76 -1.5650854323598047 2117 1 -0.7197782166587032 2117 2 0.11454266035747906 2117 3 0.5501068783453533 2117 4 -1.4762608234585173 2117 5 -2.0075442137721247 2117 6 -0.8592476927227466 2117 7 -1.6665956385764744 2117 8 -1.530575896414934 2117 9 -0.30429043879078677 2117 10-11 0.11026241570918671 2117 12-13 0.572657862293191 2117 14-15 -0.7494366406400061 2117 16-17 0.08742663767991132 2117 18-19 0.2230652377965825 2117 20-21 -0.08721071124864466 2117 22-23 0.4747054606480319 2117 24-25 0.8992225617101823 2117 26-27 1.3527621415164361 2117 28-29 0.858251195672004 2117 30-31 -0.022114184720052776 2117 32-33 1.460772627014201 2117 34-35 0.1584833188559429 2117 36-37 -1.0260431144403626 2117 38-39 0.35314080997568453 2117 40-41 -1.593439297550141 2117 42-43 -0.904746753493157 2117 44-45 0.3197383379815335 2117 46-47 -0.0017240036885155519 2117 48-49 0.3283306933801171 2117 50-51 1.133779312799028 2117 52-53 1.3791714385201956 2117 54-55 -0.2114890498768247 2117 56-57 0.2593610318816033 2117 58-59 -0.34335462382928483 2117 60-61 0.7793215501145951 2117 62-63 -0.615182394176216 2117 64-65 0.5364169721040071 2117 66-67 -1.8199892067726253 2117 68-69 0.04732009238207269 2117 70-71 1.1195089248601953 2117 72-73 0.5908357981846706 2117 74-75 -0.058947584596701574 2117 76 -0.6859645532389251 2118 1 0.6574695355890476 2118 2 1.2947374655522843 2118 3 1.5329989862374624 2118 4 1.4224654502677545 2118 5 -0.5330187392466499 2118 6 1.6542388207637657 2118 7 1.8130497160688819 2118 8 -3.6437127595518 2118 9 -0.48236236686271283 2118 10-11 -0.6821951267483541 2118 12-13 1.4131298902652247 2118 14-15 0.009491930788563252 2118 16-17 0.041722341975614086 2118 18-19 0.18884945358080074 2118 20-21 -2.477320601358535 2118 22-23 0.3445855805281468 2118 24-25 0.4732735107611319 2118 26-27 1.3081817219360161 2118 28-29 -0.6117163367955314 2118 30-31 0.6643868142809453 2118 32-33 1.4384824172240016 2118 34-35 1.9522520501246845 2118 36-37 -0.5749941633914162 2118 38-39 0.5663650856999638 2118 40-41 -0.8769682685791125 2118 42-43 1.0362497500033356 2118 44-45 0.022972603715800233 2118 46-47 0.2326041681396589 2118 48-49 -0.38920177415234747 2118 50-51 0.4350405515602631 2118 52-53 -0.8633984915497379 2118 54-55 -0.1150230159107899 2118 56-57 1.4245083845289628 2118 58-59 1.9730165050418407 2118 60-61 -1.3340650632720212 2118 62-63 0.9978920313982087 2118 64-65 0.6393765125635511 2118 66-67 -3.330104091887506 2118 68-69 1.2671627497247222 2118 70-71 -0.6650190471677853 2118 72-73 -2.4067915744427033 2118 74-75 -0.380938094087206 2118 76 0.4933561260817534 2119 1 -0.7344302313107178 2119 2 0.41857196438678557 2119 3 -0.383959055720581 2119 4 -1.2106930578907509 2119 5 -1.7987530049809166 2119 6 -0.1651084985835496 2119 7 -2.9028593748402116 2119 8 1.1214387555997192 2119 9 -0.4270010615014108 2119 10-11 -0.8302137747670031 2119 12-13 -0.8440088043734733 2119 14-15 -0.8831362743396411 2119 16-17 -0.994990944737669 2119 18-19 -1.3630153482840015 2119 20-21 -1.176038550076484 2119 22-23 0.32360655954912687 2119 24-25 -0.6960155335279126 2119 26-27 0.5578903466446405 2119 28-29 0.27858086600167553 2119 30-31 0.4559077933019182 2119 32-33 -0.3212786550370694 2119 34-35 0.5028056631782931 2119 36-37 -0.42347901187626746 2119 38-39 -0.5873353805005053 2119 40-41 -1.943256147366995 2119 42-43 -1.2252595740059817 2119 44-45 -1.8918001235569264 2119 46-47 -0.37535037731488785 2119 48-49 -1.0663579513085253 2119 50-51 0.2903727193924368 2119 52-53 -1.4605721512233956 2119 54-55 -1.5411593795471532 2119 56-57 0.7676027901233695 2119 58-59 1.3443743138996496 2119 60-61 -0.40749163669859456 2119 62-63 -0.42562195461577446 2119 64-65 -0.09270390701686892 2119 66-67 -0.02328591006932612 2119 68-69 -1.779602984541004 2119 70-71 -0.9061321007808374 2119 72-73 -2.350556143207271 2119 74-75 -0.8190208446699572 2119 76 1.3744750072006333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 5.0 13 67.0 14 162.0 15 300.0 16 474.0 17 510.0 18 546.0 19 529.0 20 491.0 21 434.0 22 448.0 23 463.0 24 537.0 25 599.0 26 689.0 27 785.0 28 945.0 29 1098.0 30 1334.0 31 1602.0 32 2044.0 33 2555.0 34 3222.0 35 4559.0 36 6992.0 37 5711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.757096677804036 22.171515497242822 11.061309863363485 33.01007796158966 2 17.093878871189457 19.824263496940784 38.67820274386135 24.403654888008408 3 16.689577100347698 28.134012560308346 28.59491657906795 26.581493760276004 4 12.97000080860354 18.646397671221802 35.53003962157354 32.853561898601114 5 12.22069485997682 34.78342901808576 33.89935581251179 19.09652030942562 6 25.6057788199779 38.236166141074364 17.802754642732 18.355300396215736 7 24.96967736718687 30.266030565213875 23.950836904665643 20.813455162933614 8 21.97245357267998 34.10689738821056 21.764911996981212 22.155737042128244 9 22.387536724077517 18.19627503301798 22.549257432414223 36.86693081049028 10-11 23.68265006334061 25.43597207622436 27.051831487021914 23.829546373413116 12-13 22.97646963693701 23.741947656397404 26.485809007843454 26.795773698822135 14-15 21.258187110859545 25.39149888143177 23.7715964529258 29.57871755478289 16-17 21.728524837605455 27.16638365542708 26.04646775019541 25.058623756772054 18-19 21.510201881350905 26.47233228214873 25.88070402415029 26.136761812350073 20-21 20.39837201153608 27.251287027303846 25.934610926929192 26.415730034230883 22-23 22.279722918519717 26.822727150211584 26.465593919301366 24.431956011967333 24-25 21.515592571628797 28.18118110023989 25.925177218942885 24.37804910918843 26-27 20.14905258618366 28.229697312740896 27.080132610980833 24.541117490094607 28-29 21.957629174415786 26.81868413250317 28.14883695857254 23.074849734508504 30-31 21.697528368507587 27.03026872591035 27.663674833562435 23.60852807201962 32-33 20.177353710142583 28.154227648850433 27.29980323980486 24.368615401202124 34-35 21.717743457049675 27.813266488773884 26.91571655750519 23.55327349667125 36-37 21.723134147327567 27.58416215196356 26.264790706449958 24.427912994258914 38-39 21.518287916767743 27.27015444327646 27.811918816204418 23.39963882375138 40-41 20.903749225088273 26.92649793806097 27.838872267593867 24.330880569256895 42-43 21.05334088029972 26.46963693700978 27.880650117247512 24.59637206544298 44-45 20.204307161532036 26.55858332659497 27.993854613083208 25.24325489878979 46-47 20.634214711193767 26.662354114444355 28.14883695857254 24.554594215789333 48-49 19.90781919624808 26.74051912347376 29.115118190884342 24.236543489393817 50-51 19.937467992776476 26.755343521737956 28.329425082881865 24.977763402603703 52-53 19.294628177138083 26.667744804722243 28.828063933586694 25.209563084552975 54-55 18.87819735317107 27.11921511549554 28.260693781838764 25.741893749494622 56-57 19.30540955769386 26.694698256111693 28.415676127328105 25.58421605886634 58-59 19.61402657610307 26.554540308886555 27.682542249535054 26.148890865475323 60-61 19.105954017411932 26.41303468909194 28.217568259615643 26.263443033880492 62-63 19.353925770194873 26.334869680062532 28.049109188431583 26.262095361311015 64-65 19.8431309129134 26.838899221045253 27.295760222096437 26.022209643944905 66-67 20.15848629416997 27.113824425217647 26.391471927980376 26.336217352632 68-69 19.618069593811487 26.731085415487456 26.49928573353818 27.151559257162877 70-71 20.124794479933154 26.557235654025497 26.03972938734805 27.278240478693295 72-73 20.666558852861108 26.690655238403277 25.666424085604163 26.97636182313145 74-75 20.473841675426538 26.98175251340934 25.985822484569148 26.55858332659497 76 21.479205412253037 27.09900002695345 25.02358426996577 26.39821029082774 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.5 12 1.0 13 1.5 14 2.0 15 3.5 16 4.0 17 3.0 18 3.5 19 5.0 20 6.0 21 6.0 22 14.0 23 30.0 24 45.0 25 52.0 26 59.0 27 95.0 28 151.5 29 179.0 30 228.0 31 320.5 32 436.0 33 508.0 34 626.5 35 837.5 36 1009.0 37 1088.0 38 1267.5 39 1638.0 40 1923.0 41 2113.5 42 2210.0 43 2356.0 44 2626.0 45 2681.0 46 2612.0 47 2640.0 48 2607.0 49 2466.0 50 2386.0 51 2161.0 52 1822.0 53 1548.5 54 1389.0 55 1310.0 56 1037.5 57 772.5 58 701.0 59 596.5 60 419.0 61 304.5 62 263.0 63 220.0 64 179.0 65 150.0 66 112.0 67 105.0 68 81.5 69 55.5 70 40.5 71 28.0 72 28.5 73 21.5 74 11.5 75 9.0 76 6.5 77 5.0 78 5.0 79 4.0 80 3.0 81 1.0 82 0.5 83 1.0 84 1.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.5 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7762594000161721 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 37101.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5587720007547 #Duplication Level Percentage of deduplicated Percentage of total 1 79.79617933118921 55.50524244629524 2 10.353779982175379 14.403924422522305 3 4.522028906885729 9.436403331446591 4 2.375324524353857 6.608986280693243 5 1.0694772736079359 3.719576291744158 6 0.6858604254659588 2.8624565375596345 7 0.38749176579997674 1.8867415972615293 8 0.282868989033983 1.5740815611439043 9 0.17437129460998954 1.091614781272742 >10 0.3526175068779789 2.9109727500606453 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0026953451389450417 0.0 2 0.0 0.0 0.0 0.0026953451389450417 0.0 3 0.0 0.0 0.0 0.0026953451389450417 0.0 4 0.0 0.0 0.0 0.005390690277890083 0.0 5 0.0 0.0 0.0 0.008086035416835126 0.0 6 0.0 0.0 0.0 0.008086035416835126 0.0 7 0.0 0.0 0.0 0.008086035416835126 0.0 8 0.0 0.0 0.0 0.010781380555780167 0.0 9 0.0 0.0 0.0 0.03503948680628555 0.0 10 0.0 0.0 0.0 0.05660224791784588 0.0 11 0.0 0.0 0.0 0.05929759305679092 0.0 12 0.0 0.0 0.0 0.05929759305679092 0.0 13 0.0 0.0 0.0 0.05929759305679092 0.0 14 0.0 0.0 0.0 0.05929759305679092 0.0 15 0.0 0.0 0.0 0.05929759305679092 0.0 16 0.0 0.0 0.0 0.06199293819573597 0.0 17 0.0 0.0 0.0 0.07277431875151613 0.0 18 0.0 0.0 0.0 0.07277431875151613 0.0 19 0.0 0.0 0.0 0.0835556993072963 0.0 20 0.0 0.0 0.0 0.09433707986307646 0.0 21 0.0 0.0 0.0 0.09703242500202151 0.0 22 0.0 0.0 0.0 0.09972777014096655 0.0 23 0.0 0.0 0.0 0.10511846041885664 0.0 24 0.0 0.0 0.0 0.12129053125252688 0.0 25 0.0 0.0 0.0 0.12129053125252688 0.0 26 0.0 0.0 0.0 0.12398587639147193 0.0 27 0.0 0.0 0.0 0.1482439826419773 0.0 28 0.0 0.0 0.0 0.17789277917037277 0.0 29 0.0 0.0 0.0 0.19676019514298806 0.0 30 0.0 0.0 0.0 0.21023692083771328 0.0 31 0.0 0.0 0.0 0.25336244306083394 0.0 32 0.0 0.0 0.0 0.2964879652839546 0.0 33 0.0 0.0 0.0 0.3369181423681302 0.0 34 0.0 0.0 0.0 0.3665669388965257 0.0 35 0.0 0.0 0.0 0.4366459125090968 0.0 36 0.0 0.0 0.0 0.5255923020942832 0.0 37 0.0 0.0 0.0 0.7951268159887873 0.0 38 0.0 0.0 0.0 1.0457939139106762 0.0 39 0.0 0.0 0.0 1.3611492951672461 0.0 40 0.0 0.0 0.0 1.7573650305921673 0.0 41 0.0 0.0 0.0 2.3341688903264064 0.0 42 0.0 0.0 0.0 2.894800679226975 0.0 43 0.0 0.0 0.0 3.5982857604916307 0.0 44 0.0 0.0 0.0 4.274817390366836 0.0 45 0.0 0.0 0.0 4.989083852187273 0.0 46 0.0 0.0 0.0 5.563192366782567 0.0 47 0.0 0.0 0.0 6.304412279992453 0.0 48 0.0 0.0 0.0 6.937818387644538 0.0 49 0.0 0.0 0.0 7.565833805018733 0.0 50 0.0 0.0 0.0 8.123770248780357 0.0 51 0.0 0.0 0.0 8.749090321015606 0.0 52 0.0 0.0 0.0 9.417535915473977 0.0 53 0.0 0.0 0.0 10.180318589795423 0.0 54 0.0 0.0 0.0 10.859545564809574 0.0 55 0.0 0.0 0.0 11.549553920379505 0.0 56 0.0 0.0 0.0 12.15600657664214 0.0 57 0.0 0.0 0.0 12.775935958599499 0.0 58 0.0 0.0 0.0 13.366216544028463 0.0 59 0.0 0.0 0.0 13.805557801676505 0.0 60 0.0 0.0 0.0 14.231422333629821 0.0 61 0.0 0.0 0.0 14.73814721975149 0.0 62 0.0 0.0 0.0 15.247567451012102 0.0 63 0.0 0.0 0.0 15.818980620468452 0.0 64 0.0 0.0 0.0 16.34187757742379 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACCAC 25 2.362024E-4 55.994606 56 TATACAA 25 2.362024E-4 55.994606 2 ATATACA 20 0.0067195497 52.851086 1 CTGTAGT 20 0.0069020195 52.494946 63 CACGGTA 20 0.0069020195 52.494946 38 AGTCACC 20 0.0069020195 52.494946 8 CCGGCTT 20 0.0069020195 52.494946 32 ATCCGAC 20 0.0069020195 52.494946 11 TAATTCC 20 0.0069020195 52.494946 4 CGGTACC 20 0.0069020195 52.494946 40 ACGGTAC 20 0.0069020195 52.494946 39 ACCTGTA 35 0.0012407172 39.99615 61 TCCTACG 35 0.0012407172 39.99615 8 ACGAACG 125 3.3323886E-9 27.997303 70 GACGAAC 160 4.6009518E-8 21.872894 69 CGACGAA 180 1.596527E-7 19.442574 68 CCGACGA 210 8.055067E-7 16.665062 67 GCCGACG 250 4.946829E-6 13.9986515 66 TGCCGAC 275 1.3235562E-5 12.726047 65 CTGCCGA 320 6.245022E-5 10.936447 64 >>END_MODULE