FastQCFastQC Report
Wed 25 May 2016
SRR1780686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37101
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5381.4500956847524327No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2740.7385245680709415RNA PCR Primer, Index 47 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC740.1994555402819331No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC550.1482439826419773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTGG150.002207926270.059
GGCGTAG150.002207926270.033
GGTCAAG150.002207926270.064
GCCATAG150.002207926270.01
ACAGGTG150.002207926270.058
GGTGGTC150.002207926270.061
CCATAGA150.002207926270.02
GTCAAGT150.002207926270.065
CATCCTT150.002207926270.014
TACAGGT150.002207926270.057
TCAAGTA150.002207926270.066
AAGGCCC150.002207926270.08
GTGGTCA150.002207926270.062
ATCCTTT200.006899392752.515
ACAAAGT200.006899392752.541
CAAGTAT200.006899392752.567
TTCAGCA200.006899392752.518
CTTCAGC200.006899392752.517
TGTGGAT200.006899392752.535
ATTTTTC200.006899392752.539
Produced by FastQC (version 0.11.5)