##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37101 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70992695614674 34.0 34.0 34.0 31.0 34.0 2 33.26662354114444 34.0 34.0 34.0 32.0 34.0 3 33.43540605374518 34.0 34.0 34.0 33.0 34.0 4 33.484461335273984 34.0 34.0 34.0 34.0 34.0 5 33.50432602894801 34.0 34.0 34.0 34.0 34.0 6 37.20115360771947 38.0 37.0 38.0 36.0 38.0 7 36.86339990835827 38.0 37.0 38.0 35.0 38.0 8 36.831702649524274 38.0 37.0 38.0 35.0 38.0 9 36.681086763160025 38.0 37.0 38.0 34.0 38.0 10-11 36.36647260181666 38.0 37.0 38.0 33.5 38.0 12-13 36.26578798415137 38.0 37.0 38.0 34.0 38.0 14-15 36.49913748955554 38.0 37.0 38.0 34.0 38.0 16-17 36.632489690304844 38.0 37.0 38.0 34.0 38.0 18-19 36.6755343521738 38.0 37.0 38.0 34.0 38.0 20-21 36.414719279803776 38.0 37.0 38.0 34.0 38.0 22-23 36.45283146006847 38.0 37.0 38.0 34.0 38.0 24-25 35.82190506994421 38.0 36.5 38.0 32.5 38.0 26-27 35.980970863319044 38.0 37.0 38.0 33.0 38.0 28-29 35.86282040915339 38.0 37.0 38.0 31.5 38.0 30-31 35.59257162879707 38.0 36.0 38.0 31.5 38.0 32-33 36.08254494488019 38.0 37.0 38.0 33.5 38.0 34-35 36.1366944287216 38.0 37.0 38.0 33.0 38.0 36-37 36.27538341284601 38.0 37.0 38.0 34.0 38.0 38-39 35.8742756259939 38.0 36.5 38.0 32.5 38.0 40-41 35.446834317134304 38.0 36.0 38.0 31.0 38.0 42-43 35.83221476510067 38.0 36.5 38.0 32.5 38.0 44-45 36.05096897657745 38.0 37.0 38.0 33.0 38.0 46-47 36.32278105711437 38.0 37.0 38.0 34.0 38.0 48-49 36.10719387617584 38.0 37.0 38.0 33.5 38.0 50-51 36.24111209940433 38.0 37.0 38.0 34.0 38.0 52-53 36.19731274089648 38.0 37.0 38.0 34.0 38.0 54-55 36.348993288590606 38.0 37.0 38.0 34.0 38.0 56-57 36.43010970054715 38.0 37.0 38.0 34.0 38.0 58-59 36.48265545403089 38.0 37.0 38.0 34.0 38.0 60-61 36.13711220721814 38.0 37.0 38.0 33.0 38.0 62-63 36.432993719845825 38.0 37.0 38.0 34.0 38.0 64-65 36.4729117813536 38.0 37.0 38.0 34.0 38.0 66-67 35.80953343575645 38.0 36.5 38.0 31.5 38.0 68-69 35.21655750518854 38.0 36.0 38.0 29.0 38.0 70-71 35.14591250909679 38.0 36.0 38.0 28.5 38.0 72-73 34.809829923721736 38.0 35.5 38.0 26.0 38.0 74-75 35.42509635858872 38.0 36.5 38.0 31.0 38.0 76 34.88967952346298 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8376018249543407 1101 2 0.5351759321117697 1101 3 0.19050538269099349 1101 4 0.023119104019237113 1101 5 0.041691996015742916 1101 6 -0.761878438301963 1101 7 0.05912970731050393 1101 8 0.26716263636444637 1101 9 0.21721765938637105 1101 10-11 0.2403106617873334 1101 12-13 0.5038062856032468 1101 14-15 0.9060864926410659 1101 16-17 -0.09435316522551318 1101 18-19 -0.3785390216997726 1101 20-21 0.023450872061893335 1101 22-23 0.748899849652922 1101 24-25 -2.0831731343502042 1101 26-27 -0.31555344662718454 1101 28-29 0.2760980560223416 1101 30-31 0.29697822606391355 1101 32-33 0.36004992502422795 1101 34-35 0.8685443077026918 1101 36-37 0.6945939428421894 1101 38-39 0.3906512060502436 1101 40-41 0.9944668524436153 1101 42-43 0.29988320663269974 1101 44-45 0.28198648476197974 1101 46-47 0.20465528800360744 1101 48-49 -1.0974951073852424 1101 50-51 -1.426414273124827 1101 52-53 -2.2245679996836643 1101 54-55 -0.6908353670252936 1101 56-57 -0.4240045093101372 1101 58-59 -1.3684634158605533 1101 60-61 -1.0185625952252408 1101 62-63 -0.4815471923429939 1101 64-65 -1.666186318497914 1101 66-67 -2.082497262669527 1101 68-69 -1.5521954732889114 1101 70-71 -0.7586667928479756 1101 72-73 -2.1701609948359106 1101 74-75 -1.2347557332877557 1101 76 -1.5271129350178896 1102 1 -0.91870069605406 1102 2 0.5444196295907631 1102 3 0.09434691930564298 1102 4 0.2102739659640136 1102 5 0.177106161682012 1102 6 0.4982656193210886 1102 7 -0.38600834791158434 1102 8 0.28372926301510404 1102 9 0.29008680704543366 1102 10-11 -0.006868209761286437 1102 12-13 0.7665313756392536 1102 14-15 -0.07482587230490623 1102 16-17 -0.10767849535756824 1102 18-19 -0.5890432233804361 1102 20-21 0.025791808436444796 1102 22-23 0.40448208254608176 1102 24-25 -5.58581419077278 1102 26-27 -1.6689748151746073 1102 28-29 -0.2811048251301216 1102 30-31 1.0554416114180611 1102 32-33 0.05302711590058351 1102 34-35 0.8088204181468726 1102 36-37 -2.6525249046968256 1102 38-39 -0.42513510847556546 1102 40-41 0.9837825787341288 1102 42-43 0.6189108176771185 1102 44-45 0.6966323431053212 1102 46-47 1.0997933432256985 1102 48-49 -2.387891265848623 1102 50-51 -0.5958020282268706 1102 52-53 -0.5350121773547301 1102 54-55 0.21552717799271903 1102 56-57 0.09364254951339035 1102 58-59 -0.4970948684415859 1102 60-61 -5.615801490783461 1102 62-63 -2.747333506868806 1102 64-65 0.29275725893305804 1102 66-67 1.027946915001543 1102 68-69 -0.3044763856538566 1102 70-71 -1.1099873210592648 1102 72-73 -5.561997729529786 1102 74-75 -2.6164484103585792 1102 76 -0.39650069011992883 1103 1 0.8349034979171037 1103 2 0.7311824080966645 1103 3 0.18262626774226476 1103 4 0.5327791148125414 1103 5 0.4047788821463456 1103 6 0.4200967370892954 1103 7 0.7613423354810394 1103 8 0.7851334944324435 1103 9 0.3177033582530697 1103 10-11 0.49022971197621246 1103 12-13 -0.7492428239488333 1103 14-15 -0.5583495570081425 1103 16-17 -0.11788257699021898 1103 18-19 0.4414285983757864 1103 20-21 -0.26624151865586754 1103 22-23 0.3765846850549721 1103 24-25 -3.3056232153000167 1103 26-27 -1.0613170731787278 1103 28-29 0.36357781856955995 1103 30-31 1.4182950096580882 1103 32-33 0.6493105834160318 1103 34-35 0.5531098770496996 1103 36-37 -0.8787465860299051 1103 38-39 -0.8021712440307027 1103 40-41 -1.0405574324997247 1103 42-43 -0.246797851111495 1103 44-45 1.1561904782859926 1103 46-47 1.0327178891908773 1103 48-49 -2.802513995674502 1103 50-51 -0.6355885850514369 1103 52-53 -0.13152968343973725 1103 54-55 -0.16277276583802802 1103 56-57 0.5202480541005485 1103 58-59 -0.258047873496821 1103 60-61 -5.076576626525835 1103 62-63 -1.0486815690294478 1103 64-65 0.327769428938673 1103 66-67 -0.7241594321244591 1103 68-69 0.4316685310284143 1103 70-71 -0.23135972323113663 1103 72-73 -5.025206866395543 1103 74-75 -2.3023218423001666 1103 76 -4.7619753203389905 1104 1 0.0516982743449077 1104 2 -0.12417753900640349 1104 3 -0.024332985088548753 1104 4 -0.4031660046188179 1104 5 0.06458547773275569 1104 6 0.048826384167568904 1104 7 0.20104809628772102 1104 8 0.5126316347746211 1104 9 0.9314730912888578 1104 10-11 -0.7997529312174407 1104 12-13 0.6839334716127823 1104 14-15 0.7055565509346593 1104 16-17 0.09382913472149568 1104 18-19 0.5650751807379635 1104 20-21 -0.3057489159614235 1104 22-23 -0.25565764902222554 1104 24-25 0.5472985137684958 1104 26-27 0.9883151278296225 1104 28-29 0.9588749505639456 1104 30-31 0.4650757353378907 1104 32-33 0.4485501899950819 1104 34-35 0.23605888109961626 1104 36-37 1.2393210228633933 1104 38-39 1.4096231191398019 1104 40-41 1.4981234502178538 1104 42-43 0.34068871802644907 1104 44-45 0.581721513908775 1104 46-47 0.8122864485759465 1104 48-49 -0.3850414772845454 1104 50-51 0.16187401516345545 1104 52-53 0.34437741632058305 1104 54-55 -0.1940150172637658 1104 56-57 0.27382272969357047 1104 58-59 -0.5595603951928254 1104 60-61 0.3277543099151927 1104 62-63 0.031118414440257425 1104 64-65 0.6757320704792988 1104 66-67 -0.1802715075025887 1104 68-69 1.263596789562179 1104 70-71 1.580442905085249 1104 72-73 0.9172263925514841 1104 74-75 -0.32765451442182325 1104 76 -0.33251834042329165 1105 1 0.4661126887593241 1105 2 0.5064530916242234 1105 3 0.39909043833487345 1105 4 -0.06082366227646929 1105 5 -0.31379290064562326 1105 6 -0.06829073294954924 1105 7 0.5974444926841187 1105 8 -0.036917914774932115 1105 9 0.5981397579555292 1105 10-11 0.5515984201339137 1105 12-13 -0.5638142761349627 1105 14-15 0.31816916354726743 1105 16-17 0.17941472030707928 1105 18-19 0.47498509064786987 1105 20-21 0.4555123452998373 1105 22-23 0.1902882969237254 1105 24-25 0.6103615768315649 1105 26-27 -0.08375694424245239 1105 28-29 0.7967127884017771 1105 30-31 0.7578685281306861 1105 32-33 0.5971988386437275 1105 34-35 -1.4711483261075884 1105 36-37 0.43751922106159213 1105 38-39 0.07628978580647328 1105 40-41 0.2819072340016433 1105 42-43 0.3001481774859087 1105 44-45 0.7979377301249926 1105 46-47 0.7267008629903557 1105 48-49 -0.3039603962034647 1105 50-51 0.6168289701184122 1105 52-53 0.5605936325367935 1105 54-55 0.5627417394929921 1105 56-57 0.32787678374762663 1105 58-59 0.4404396048071746 1105 60-61 0.7331597153205962 1105 62-63 -0.29320590988406536 1105 64-65 0.603659998407224 1105 66-67 -0.13522646245754544 1105 68-69 0.1735066994720853 1105 70-71 1.3867492113915532 1105 72-73 0.9262354015604899 1105 74-75 -0.31864550541281744 1105 76 -0.17035617826113025 1106 1 -1.035048266009877 1106 2 0.3257156531548304 1106 3 0.4695836119208323 1106 4 0.3919486977123796 1106 5 0.29724081348087594 1106 6 -0.20602640676769823 1106 7 -0.4844724758307919 1106 8 -0.10676095369013439 1106 9 -0.7211692417658426 1106 10-11 0.4296981693676827 1106 12-13 1.4105982807518131 1106 14-15 -0.45279767958452055 1106 16-17 0.4336638147623617 1106 18-19 0.2419582493736101 1106 20-21 0.05656184841119227 1106 22-23 0.1763104015025334 1106 24-25 -0.38857034478077424 1106 26-27 1.283476268351592 1106 28-29 0.8226717370411407 1106 30-31 0.7465840477740358 1106 32-33 0.6256654495845666 1106 34-35 0.9980167152223984 1106 36-37 1.1920469446614774 1106 38-39 0.15086952018001654 1106 40-41 1.0392212490476211 1106 42-43 1.1525211690341592 1106 44-45 1.3272866666904264 1106 46-47 1.0977420072273816 1106 48-49 1.3259736615656408 1106 50-51 0.2962661392463062 1106 52-53 0.6266751779997861 1106 54-55 0.3507049604459027 1106 56-57 0.45962193095668624 1106 58-59 0.6265638734056651 1106 60-61 1.1472955005904026 1106 62-63 0.8364030805496654 1106 64-65 -0.8551493263814791 1106 66-67 0.933269868925386 1106 68-69 1.472243580683191 1106 70-71 0.33399542667901017 1106 72-73 -0.20211975772250668 1106 74-75 -0.5871511244339018 1106 76 -0.07144283192510414 1107 1 1.0902278753745094 1107 2 -0.29486608469495224 1107 3 -0.04468369293925889 1107 4 0.4092535578007457 1107 5 0.31868779433506944 1107 6 0.261020721361902 1107 7 0.7185834888231142 1107 8 -0.15121971657673328 1107 9 -0.609147886832119 1107 10-11 0.4676215861570796 1107 12-13 0.010791579720887512 1107 14-15 0.11968943381754116 1107 16-17 0.600867423009781 1107 18-19 0.6207782051909945 1107 20-21 0.3402050737425597 1107 22-23 0.20677800091343101 1107 24-25 0.8886756051455933 1107 26-27 0.059341511356009846 1107 28-29 0.5409615014004956 1107 30-31 0.9342681420303052 1107 32-33 0.9580016056965022 1107 34-35 -0.23964896960823268 1107 36-37 0.9641842789766457 1107 38-39 0.041701626218312526 1107 40-41 0.6419458440402508 1107 42-43 1.2560281646158984 1107 44-45 0.9070915267787854 1107 46-47 1.1782372207767153 1107 48-49 0.6573893463962719 1107 50-51 1.2396571554466007 1107 52-53 0.7634572104003752 1107 54-55 0.32203228003353246 1107 56-57 0.848106835227675 1107 58-59 0.49015002951760067 1107 60-61 0.881647488808369 1107 62-63 0.9382532278250721 1107 64-65 1.1352317487289767 1107 66-67 0.03751324153216018 1107 68-69 1.2688654510808348 1107 70-71 0.4174361483284912 1107 72-73 0.8066247194498075 1107 74-75 -0.4806065736238878 1107 76 1.0282442078392577 1108 1 -0.7105726172363269 1108 2 0.2156018956006136 1108 3 0.2998360114942429 1108 4 0.5188594691307955 1108 5 0.4464587302956602 1108 6 0.11631628786436465 1108 7 -0.13671207767934845 1108 8 -0.7611335096801781 1108 9 -0.27324887205379156 1108 10-11 0.3056757733492006 1108 12-13 1.106696752134681 1108 14-15 0.5711980545071924 1108 16-17 0.24849673335460665 1108 18-19 0.6960552987870514 1108 20-21 1.2307531032992145 1108 22-23 -0.18269244243632698 1108 24-25 1.1004982652441129 1108 26-27 0.4260287034742305 1108 28-29 -0.24829342470788163 1108 30-31 0.6568666641977927 1108 32-33 0.9769363347605378 1108 34-35 1.0300554638893047 1108 36-37 1.3076263972032507 1108 38-39 1.0256917740015652 1108 40-41 1.2441625928087277 1108 42-43 0.3802584601823966 1108 44-45 1.0705767484536608 1108 46-47 0.9625660385107082 1108 48-49 1.1387624991056384 1108 50-51 1.0028960717027573 1108 52-53 0.04424538774027553 1108 54-55 -0.0346487544492291 1108 56-57 0.6884701357202871 1108 58-59 0.3786968275471523 1108 60-61 1.1525834493995006 1108 62-63 1.1517692376772928 1108 64-65 1.0965925861674037 1108 66-67 1.1297238326651566 1108 68-69 1.4657558451695039 1108 70-71 -0.3918310930508184 1108 72-73 -0.3968851327669398 1108 74-75 -0.01431962781107643 1108 76 1.1418525822234926 1109 1 -1.053547158003326 1109 2 0.38487356817554286 1109 3 0.537509230959273 1109 4 0.31679694898900124 1109 5 0.17825054466884893 1109 6 -0.26017420520685164 1109 7 0.2865073873544901 1109 8 0.5444578535167253 1109 9 0.5314562520196873 1109 10-11 0.3704920801490701 1109 12-13 0.058396719907804595 1109 14-15 0.7099347609203477 1109 16-17 0.8026781706733317 1109 18-19 0.6589541063925921 1109 20-21 0.8635278374274762 1109 22-23 0.2053594428359844 1109 24-25 -0.2605233267636251 1109 26-27 0.3077560640929917 1109 28-29 -1.5109827576115222 1109 30-31 0.2202327615229578 1109 32-33 0.7828096831330811 1109 34-35 -0.23013628603013103 1109 36-37 1.363258043996673 1109 38-39 1.403350491371846 1109 40-41 0.2884745489801688 1109 42-43 0.8540338388482098 1109 44-45 0.6680227684343265 1109 46-47 0.7053149911372003 1109 48-49 0.42795843851910575 1109 50-51 0.13059507266955706 1109 52-53 -0.3495267682378085 1109 54-55 -0.004194355480485967 1109 56-57 -0.06990885235576627 1109 58-59 -0.1508713322981592 1109 60-61 0.7189305328671196 1109 62-63 0.5087221864365503 1109 64-65 0.6210044456582153 1109 66-67 0.8331157114921055 1109 68-69 -0.5715467652075503 1109 70-71 1.1741534380481227 1109 72-73 0.721427790210825 1109 74-75 0.8033970979382019 1109 76 1.4560646350755704 1110 1 -2.442077319430684 1110 2 -0.5867492015780655 1110 3 -0.20361226436782687 1110 4 -0.0030841045369172093 1110 5 0.39677220991949014 1110 6 -0.12177148643030478 1110 7 0.5831938784335051 1110 8 0.8067348288778149 1110 9 0.9546508144665822 1110 10-11 -0.6810797125442178 1110 12-13 0.614768852448158 1110 14-15 0.9975303429084477 1110 16-17 0.5412083321006875 1110 18-19 -0.1870951714323894 1110 20-21 0.7547342945217821 1110 22-23 -0.6583194016839755 1110 24-25 -1.1957399792699945 1110 26-27 0.7432700827845764 1110 28-29 0.1525686442576344 1110 30-31 -0.13992016965801213 1110 32-33 0.7616542030990914 1110 34-35 0.9795068745476101 1110 36-37 0.814914798457167 1110 38-39 0.6705977301144159 1110 40-41 -1.721041168946762 1110 42-43 -0.14583871850098262 1110 44-45 0.68679931898658 1110 46-47 0.33059111688061193 1110 48-49 1.2626653204222436 1110 50-51 0.1872220905115327 1110 52-53 0.4540416259847859 1110 54-55 -0.5421722654210086 1110 56-57 0.27546073133157734 1110 58-59 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-0.17741754112804387 2116 48-49 0.2243536321105637 2116 50-51 -1.6978428445533993 2116 52-53 0.16226673420989357 2116 54-55 -0.04552724377599304 2116 56-57 0.11745207332290875 2116 58-59 -1.5396118752443115 2116 60-61 -1.5826382254773392 2116 62-63 0.40997941830126194 2116 64-65 -1.4198277750805488 2116 66-67 -0.3776653298964163 2116 68-69 -1.7594083584429754 2116 70-71 1.186781386423732 2116 72-73 1.0715056718307565 2116 74-75 0.8810005692332581 2116 76 -0.6056843635893188 2117 1 0.7736069962536334 2117 2 0.33013391530504776 2117 3 0.2568272960717337 2117 4 -0.049537650990458815 2117 5 0.13942955257682854 2117 6 0.4629437982849822 2117 7 0.8353417305813551 2117 8 -0.20135707921409818 2117 9 -0.46423030441453506 2117 10-11 -0.9032575347220444 2117 12-13 -1.1640023763230687 2117 14-15 0.17635152172962876 2117 16-17 -0.7882122473198905 2117 18-19 -1.2786036629408741 2117 20-21 -0.1347262449387543 2117 22-23 -0.2540736474382186 2117 24-25 -1.317368350898363 2117 26-27 -2.551235411720917 2117 28-29 -2.017761026072037 2117 30-31 0.07506484532700597 2117 32-33 -2.295089053644162 2117 34-35 0.061175206215942524 2117 36-37 -1.1309393473969749 2117 38-39 0.4441741536908381 2117 40-41 0.468868920963331 2117 42-43 -0.02041414307640821 2117 44-45 -0.36694693475967455 2117 46-47 -1.0858424495529562 2117 48-49 -0.4075144997575677 2117 50-51 -0.2802604269709832 2117 52-53 0.8032923752355359 2117 54-55 -0.28133310458185434 2117 56-57 -0.05470909883825925 2117 58-59 -0.6788060144384431 2117 60-61 -0.49884701668612763 2117 62-63 -0.11703523371338775 2117 64-65 -1.091073196325965 2117 66-67 -0.8213466485777374 2117 68-69 0.3701703961357836 2117 70-71 -0.313676489034151 2117 72-73 1.255113730438822 2117 74-75 0.997758810991499 2117 76 0.6003595924546374 2118 1 0.6851954078420448 2118 2 0.12234170751284523 2118 3 -1.0877031734587348 2118 4 0.16737044091762954 2118 5 0.2967722099194887 2118 6 0.25777396811515274 2118 7 0.7559211511607771 2118 8 0.8430984652414466 2118 9 -1.470349185533415 2118 10-11 -0.2969888034533028 2118 12-13 -0.1454584202791125 2118 14-15 0.8554848883629944 2118 16-17 -1.7258371224447657 2118 18-19 -0.4291406259778441 2118 20-21 -0.06685661456912584 2118 22-23 0.7814533255887568 2118 24-25 1.3497145661845522 2118 26-27 -1.5340026444881474 2118 28-29 0.1525686442576344 2118 30-31 -0.6444656242034625 2118 32-33 0.2684723849172812 2118 34-35 0.4454159654566965 2118 36-37 -3.3032670197246503 2118 38-39 0.3842340937507842 2118 40-41 0.46418610378051284 2118 42-43 0.5746158269535613 2118 44-45 -1.3120643173770503 2118 46-47 0.7476365714260709 2118 48-49 0.4978925931495226 2118 50-51 -0.6150506367611897 2118 52-53 -1.9402765558333925 2118 54-55 -0.4535359017846474 2118 56-57 0.5311425495133903 2118 58-59 -0.7192655548979943 2118 60-61 -0.6191641995033166 2118 62-63 -1.29023703191519 2118 64-65 0.7533323980796212 2118 66-67 -0.0611068883379744 2118 68-69 -0.9758585748931878 2118 70-71 1.0680043301466782 2118 72-73 1.893475368800452 2118 74-75 0.9053512185839026 2118 76 0.5047052468002917 2119 1 0.7259879486345824 2119 2 -0.6515510663799304 2119 3 0.324592863837303 2119 4 -0.5916621931150061 2119 5 -0.37339096024368246 2119 6 -0.4546386192974339 2119 7 -1.2708853756457543 2119 8 -0.031027408884426677 2119 9 0.36910302891880065 2119 10-11 -0.0845762160407233 2119 12-13 0.4202467079260117 2119 14-15 -0.33463748925937864 2119 16-17 -0.15634411545175197 2119 18-19 -0.13849377283099074 2119 20-21 -0.7922354024479077 2119 22-23 -0.03978793315251039 2119 24-25 0.6981994146694035 2119 26-27 0.45609059560509024 2119 28-29 0.1525686442576344 2119 30-31 0.6877022079643638 2119 32-33 0.9430061844510718 2119 34-35 0.5785744736152125 2119 36-37 0.4688775024198719 2119 38-39 -1.5219430624263808 2119 40-41 -0.5091530570586471 2119 42-43 0.006142633480365589 2119 44-45 -0.015298583111324149 2119 46-47 -0.5537911675016716 2119 48-49 -0.8571481993912755 2119 50-51 0.2517908550802943 2119 52-53 0.531314353257514 2119 54-55 0.08405151080275886 2119 56-57 -0.9704600145891717 2119 58-59 -0.25481334044577153 2119 60-61 0.03686726902814996 2119 62-63 -0.39542351210166515 2119 64-65 -0.052611657864432004 2119 66-67 0.7052101279790435 2119 68-69 1.1036868796522654 2119 70-71 -0.278877954235611 2119 72-73 0.8759928513179389 2119 74-75 0.2917148549475428 2119 76 0.35493834703339644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 6.0 23 9.0 24 13.0 25 15.0 26 68.0 27 97.0 28 202.0 29 312.0 30 554.0 31 902.0 32 1385.0 33 2031.0 34 3219.0 35 4997.0 36 8531.0 37 14760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.90202420419935 24.14220640953074 11.740923425244603 29.21484596102531 2 17.06962076493895 19.387617584431688 38.2442521764912 25.298509474138164 3 17.10196490660629 25.19069566858036 30.44661868952319 27.260720735290157 4 13.17754238430231 19.557424328185224 37.26045120077626 30.00458208673621 5 12.943047357214091 36.51653594242743 36.7779844209051 13.762432279453385 6 27.160992965149187 38.75636775289076 17.91865448370664 16.163984798253416 7 26.619228592221233 29.074688013800166 22.980512654645427 21.325570739333173 8 21.845772351149563 36.4734104202043 21.018301393493438 20.662515835152693 9 22.530390016441608 16.37961240936902 23.096412495620065 37.99358507856931 10-11 24.379396781757904 26.00738524568071 27.006010619659847 22.60720735290154 12-13 23.64626290396485 23.09236947791165 26.705479636667473 26.555887981456028 14-15 21.515592571628797 25.022236597396297 23.807983612301555 29.65418721867335 16-17 22.608555025471013 27.902212878359073 25.28098973073502 24.20824236543489 18-19 22.074876687959893 27.7930514002318 26.324088299506755 23.807983612301555 20-21 21.38891135009838 26.840246893614726 26.496590388399234 25.274251367887658 22-23 22.25546481226921 27.943990728012725 26.361823131451985 23.438721328266084 24-25 22.309371715048112 29.012695075604434 25.98717015713862 22.690763052208833 26-27 21.37408695183418 29.553111775962915 26.46155090159295 22.611250370609955 28-29 21.57758550982453 27.662327160992966 28.11918816204415 22.64089916713835 30-31 21.223147624053258 27.588205169671976 28.46823535753753 22.720411848737232 32-33 23.254090186248348 27.542384302309912 26.77016792000216 22.433357591439584 34-35 21.77973639524541 27.884693134955928 28.58413519851217 21.751435271286486 36-37 22.750060645265627 27.17716503598286 26.78364464569688 23.289129673054635 38-39 21.36869626155629 27.103043044661867 27.73375380717501 23.794506886606833 40-41 20.96574216328401 26.125980431794293 28.943963774561333 23.96431363036037 42-43 21.85924907684429 26.031643351931216 28.98708929678445 23.12201827444004 44-45 20.29325355111722 26.516805476941325 29.092207757203308 24.09773321473815 46-47 21.79051777580119 25.541090536643217 28.933182394005552 23.73520929355004 48-49 21.39969273065416 25.020888924826824 28.89409988949085 24.685318455028167 50-51 19.682757877146166 25.975041104013368 29.832079997843724 24.51012102099674 52-53 19.33505835422226 25.892833077275547 31.664914692326352 23.107193876175845 54-55 18.961753052478368 26.320045281798333 29.861728794372116 24.856472871351176 56-57 20.12209913479421 25.68798684671572 29.084121721786477 25.105792296703594 58-59 18.705695264278592 27.135387186329208 30.036926228403548 24.121991320988652 60-61 19.47117328373898 28.073367294682082 28.206786879059862 24.24867254251907 62-63 18.09654726287701 29.617800059297593 27.37662057626479 24.909032101560605 64-65 19.01026926497938 30.074661060348777 26.051858440473303 24.86321123419854 66-67 18.7003045740007 29.9803239804857 25.539742864073744 25.77962858143985 68-69 18.348562033368374 29.71887550200803 25.97234575887442 25.960216705749172 70-71 19.480606991725292 28.96283119053395 25.6057788199779 25.95078299776286 72-73 19.161208592760303 28.543705021427996 25.51413708525377 26.78094930055794 74-75 19.546642947629444 29.057168270397028 25.298509474138164 26.09767930783537 76 19.82695884207973 29.042343872132825 25.223039810247705 25.907657475539743 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 2.0 17 4.0 18 4.0 19 4.5 20 11.0 21 17.0 22 21.5 23 28.5 24 36.5 25 42.0 26 70.0 27 106.0 28 157.0 29 200.0 30 250.5 31 362.0 32 490.5 33 558.0 34 655.0 35 843.5 36 996.5 37 1058.0 38 1268.0 39 1741.5 40 2259.5 41 2587.5 42 2661.0 43 2669.5 44 2798.5 45 2908.0 46 2897.0 47 2816.5 48 2635.5 49 2356.5 50 2178.0 51 2039.5 52 1664.5 53 1308.0 54 1188.0 55 1089.0 56 851.0 57 622.5 58 533.0 59 433.0 60 275.5 61 217.0 62 216.0 63 173.0 64 111.5 65 87.0 66 64.5 67 48.0 68 39.0 69 23.5 70 10.5 71 4.0 72 4.5 73 3.0 74 1.5 75 2.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 37101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.46497398991941 #Duplication Level Percentage of deduplicated Percentage of total 1 62.26024411508283 30.79701355758605 2 16.766564952048824 16.587153985067786 3 8.418700959023539 12.492924719010269 4 4.680688753269399 9.261205897415165 5 2.757192676547515 6.819223201530956 6 1.5747602441150828 4.673728470930703 7 1.0353095030514385 3.5848090347969057 8 0.7846556233653008 3.1050376000646884 9 0.41957279860505664 1.867874181288914 >10 1.2805143853530951 8.274709576561278 >50 0.010897994768962511 0.3476995229239104 >100 0.0054489973844812556 0.7385245680709415 >500 0.0054489973844812556 1.4500956847524327 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 538 1.4500956847524327 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 274 0.7385245680709415 RNA PCR Primer, Index 47 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 74 0.1994555402819331 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 55 0.1482439826419773 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005390690277890083 0.0 2 0.0 0.0 0.0 0.005390690277890083 0.0 3 0.0 0.0 0.0 0.005390690277890083 0.0 4 0.0 0.0 0.0 0.008086035416835126 0.0 5 0.0 0.0 0.0 0.010781380555780167 0.0 6 0.0 0.0 0.0 0.010781380555780167 0.0 7 0.0 0.0 0.0 0.010781380555780167 0.0 8 0.0 0.0 0.0 0.010781380555780167 0.0 9 0.0 0.0 0.0 0.0323441416673405 0.0 10 0.0 0.0 0.0 0.05660224791784588 0.0 11 0.0 0.0 0.0 0.064688283334681 0.0 12 0.0 0.0 0.0 0.06738362847362604 0.0 13 0.0 0.0 0.0 0.06738362847362604 0.0 14 0.0 0.0 0.0 0.06738362847362604 0.0 15 0.0 0.0 0.0 0.06738362847362604 0.0 16 0.0 0.0 0.0 0.0700789736125711 0.0 17 0.0 0.0 0.0 0.0835556993072963 0.0 18 0.0 0.0 0.0 0.08625104444624133 0.0 19 0.0 0.0 0.0 0.09164173472413142 0.0 20 0.0 0.0 0.0 0.10242311527991159 0.0 21 0.0 0.0 0.0 0.10511846041885664 0.0 22 0.0 0.0 0.0 0.10781380555780168 0.0 23 0.0 0.0 0.0 0.11050915069674672 0.0 24 0.0 0.0 0.0 0.11859518611358184 0.0 25 0.0 0.0 0.0 0.11859518611358184 0.0 26 0.0 0.0 0.0 0.12398587639147193 0.0 27 0.0 0.0 0.0 0.1482439826419773 0.0 28 0.0 0.0 0.0 0.18328346944826285 0.0 29 0.0 0.0 0.0 0.19676019514298806 0.0 30 0.0 0.0 0.0 0.21293226597665832 0.0 31 0.0 0.0 0.0 0.25336244306083394 0.0 32 0.0 0.0 0.0 0.29379262014500956 0.0 33 0.0 0.0 0.0 0.33961348750707526 0.0 34 0.0 0.0 0.0 0.37195762917441577 0.0 35 0.0 0.0 0.0 0.450122638203822 0.0 36 0.0 0.0 0.0 0.5498504083447886 0.0 37 0.0 0.0 0.0 0.8247756125171828 0.0 38 0.0 0.0 0.0 1.0835287458559069 0.0 39 0.0 0.0 0.0 1.4231422333629822 0.0 40 0.0 0.0 0.0 1.8382253847605186 0.0 41 0.0 0.0 0.0 2.4824128729683834 0.0 42 0.0 0.0 0.0 3.0592167327026227 0.0 43 0.0 0.0 0.0 3.8031319910514543 0.0 44 0.0 0.0 0.0 4.582086736206572 0.0 45 0.0 0.0 0.0 5.379908897334303 0.0 46 0.0 0.0 0.0 6.018705695264279 0.0 47 0.0 0.0 0.0 6.827309236947791 0.0 48 0.0 0.0 0.0 7.563138459879788 0.0 49 0.0 0.0 0.0 8.290881647394949 0.0 50 0.0 0.0 0.0 8.881162232823913 0.0 51 0.0 0.0 0.0 9.552303172421228 0.0 52 0.0 0.0 0.0 10.293523085631115 0.0 53 0.0 0.0 0.0 11.17759629120509 0.0 54 0.0 0.0 0.0 11.916120859276031 0.0 55 0.0 0.0 0.0 12.662731462763807 0.0 56 0.0 0.0 0.0 13.350044473194792 0.0 57 0.0 0.0 0.0 14.034662138486834 0.0 58 0.0 0.0 0.0 14.659982210722083 0.0 59 0.0 0.0 0.0 15.147839680871135 0.0 60 0.0 0.0 0.0 15.641087841298079 0.0 61 0.0 0.0 0.0 16.180156869087085 0.0 62 0.0 0.0 0.0 16.756960728821326 0.0 63 0.0 0.0 0.0 17.42271097814075 0.0 64 0.0 0.0 0.0 18.00490552815288 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGTGG 15 0.0022079262 70.0 59 GGCGTAG 15 0.0022079262 70.0 33 GGTCAAG 15 0.0022079262 70.0 64 GCCATAG 15 0.0022079262 70.0 1 ACAGGTG 15 0.0022079262 70.0 58 GGTGGTC 15 0.0022079262 70.0 61 CCATAGA 15 0.0022079262 70.0 2 GTCAAGT 15 0.0022079262 70.0 65 CATCCTT 15 0.0022079262 70.0 14 TACAGGT 15 0.0022079262 70.0 57 TCAAGTA 15 0.0022079262 70.0 66 AAGGCCC 15 0.0022079262 70.0 8 GTGGTCA 15 0.0022079262 70.0 62 ATCCTTT 20 0.0068993927 52.5 15 ACAAAGT 20 0.0068993927 52.5 41 CAAGTAT 20 0.0068993927 52.5 67 TTCAGCA 20 0.0068993927 52.5 18 CTTCAGC 20 0.0068993927 52.5 17 TGTGGAT 20 0.0068993927 52.5 35 ATTTTTC 20 0.0068993927 52.5 39 >>END_MODULE