FastQCFastQC Report
Wed 25 May 2016
SRR1780685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291202
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT3220.11057616362524982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAAC357.2956846E-637.69601426
CGACAAC250.00233763235.23745724
TCAGCCG401.795816E-533.0521920
AGCCGAT401.8064287E-533.0237522
GACTATC353.1951466E-431.456638
CAGCGTC353.24058E-431.3809859
GCACGAC300.005684196529.38984721
CAGCCGA454.0060735E-529.38984521
CCTAACG300.005693778429.37972520
TAAACCG300.00570337329.36960418
AACGTCT300.005717789329.35444323
CGTTGAG300.005751538629.31912430
TTGGAAT406.9827127E-427.52452735
TCGGTCA407.039121E-427.4866829
CAGTTGG407.0532813E-427.47723432
CTAGACG407.081673E-427.4583633
TGAGCAA655.89469E-727.08242611
TATCGAT655.948077E-727.05450642
TCGATTA655.965976E-727.04521444
TCTATGG655.9749436E-727.040572
Produced by FastQC (version 0.11.5)