FastQCFastQC Report
Wed 25 May 2016
SRR1780685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291202
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT87503.0047870550339626No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT35891.2324777989162161TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC12470.4282250808716973No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG8500.29189359963187067No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC5880.20192169009828229No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3620.1243123330196908No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT3580.1229387160802467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACA207.801491E-444.0517431
TTCGTGA207.8478473E-443.998827
ATCTTCG207.8478473E-443.99887
ACATCTT207.8478473E-443.99885
AAGACGG254.4353466E-543.99879525
CCGTCTT4900.036.81532344
GAAGACG301.2989185E-436.6656724
GGTTCAC301.2989185E-436.6656738
TCCGCGA250.002350185835.19903619
CGCGAAG250.002350185835.19903621
CTGCGTT250.002350185835.19903631
AGACGGC250.002350185835.19903626
TATACAG250.002350185835.1990365
GTAACTG250.002350185835.19903641
CGAAGAC250.002350185835.19903623
TGTCCGC250.002350185835.19903617
GCGAGCC250.002350185835.19903622
GCCGTCT5500.032.79910343
CGCAAAT353.2124267E-431.42771138
CGTGCGA502.353836E-630.79915610
Produced by FastQC (version 0.11.5)