FastQCFastQC Report
Wed 25 May 2016
SRR1780684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79673
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGG150.002217920369.994988
TCGTGAC150.002217920369.9949841
GTCGCCC200.00693039152.4962337
ATCTACG200.00693039152.4962352
CACGGTT200.00693039152.4962349
CTATAAC200.00693039152.496234
GCAAAAA200.00693039152.4962313
TTCCGAC200.00693039152.4962342
CTGACTT200.00693039152.4962321
TTTCCGA200.00693039152.4962341
ACTAAAT200.00693039152.4962367
CTAATTC200.00693039152.4962330
CTTACTC200.00693039152.496233
CCATAGG305.704872E-446.8988461
GGGGCAC305.8483955E-446.6633237
CCGATGA350.001249187439.997139
CACTGGT350.001249187439.9971359
CTGGTTA350.001249187439.9971361
GCGGGGA350.001249187439.9971318
GGGCACC350.001249187439.9971338
Produced by FastQC (version 0.11.5)