##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780684_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 79673 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.742660625305938 34.0 31.0 34.0 12.0 34.0 2 31.201699446487517 34.0 32.0 34.0 23.0 34.0 3 31.525673691212834 34.0 34.0 34.0 23.0 34.0 4 31.89183286684322 34.0 34.0 34.0 23.0 34.0 5 31.84268196252181 34.0 34.0 34.0 23.0 34.0 6 34.84974834636577 38.0 36.0 38.0 26.0 38.0 7 34.036674908689264 38.0 34.0 38.0 23.0 38.0 8 32.80693585028805 37.0 32.0 38.0 12.0 38.0 9 33.58942176144993 37.0 34.0 38.0 21.0 38.0 10-11 34.017640857003 37.0 34.0 38.0 23.0 38.0 12-13 34.399256962835594 38.0 35.0 38.0 24.5 38.0 14-15 34.01178567394224 38.0 34.0 38.0 22.5 38.0 16-17 33.917312012852534 37.0 34.0 38.0 22.0 38.0 18-19 33.86688715123066 37.0 34.0 38.0 22.0 38.0 20-21 33.900537195787784 37.0 34.0 38.0 22.5 38.0 22-23 33.91763834674231 37.0 34.0 38.0 22.0 38.0 24-25 34.35619971634054 38.0 35.0 38.0 24.0 38.0 26-27 34.43209117266828 38.0 35.5 38.0 24.5 38.0 28-29 34.57127257665709 38.0 36.0 38.0 25.0 38.0 30-31 34.41423066785485 38.0 35.0 38.0 24.0 38.0 32-33 34.16085122940017 38.0 34.0 38.0 23.5 38.0 34-35 34.01844414042398 37.0 34.0 38.0 23.0 38.0 36-37 34.02579920424736 37.5 34.0 38.0 23.0 38.0 38-39 34.57159263489513 38.0 36.0 38.0 25.5 38.0 40-41 33.99912768441003 37.5 34.0 38.0 23.0 38.0 42-43 34.33791246720972 38.0 35.0 38.0 24.0 38.0 44-45 33.38856952794548 37.0 34.0 38.0 21.5 38.0 46-47 34.105179922934994 37.0 34.0 38.0 24.0 38.0 48-49 33.856218543295725 37.0 34.0 38.0 23.0 38.0 50-51 33.815056543622056 37.0 34.0 38.0 22.5 38.0 52-53 33.82258105004205 37.0 34.0 38.0 23.0 38.0 54-55 33.52699157807538 37.0 34.0 38.0 21.5 38.0 56-57 33.63343918265912 37.0 34.0 38.0 22.0 38.0 58-59 33.481185596124156 37.0 33.5 38.0 22.0 38.0 60-61 33.49973642262749 37.0 33.5 38.0 21.5 38.0 62-63 33.60735129843234 37.0 34.0 38.0 22.5 38.0 64-65 33.6454005748497 37.0 34.0 38.0 22.5 38.0 66-67 33.59048862224342 37.0 33.5 38.0 22.5 38.0 68-69 33.17087972085901 37.0 32.5 38.0 20.5 38.0 70-71 33.03572100962685 37.0 32.5 38.0 20.5 38.0 72-73 33.1289960212368 37.0 32.0 38.0 20.5 38.0 74-75 32.77010405030562 37.0 31.5 38.0 19.5 38.0 76 32.01981850815207 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.3542056230916266 1101 2 -1.190650915217521 1101 3 -0.4734369246762 1101 4 -2.7534677599449786 1101 5 -1.5095569841374328 1101 6 -2.772263838770776 1101 7 -1.6439427322797826 1101 8 -1.864373635403286 1101 9 -0.4721925982553472 1101 10-11 -1.4220003643147123 1101 12-13 -1.7603851558974597 1101 14-15 -1.4940860564439618 1101 16-17 -2.713104687540362 1101 18-19 -0.7686698558643243 1101 20-21 -1.5860528926917823 1101 22-23 -1.2280480639502471 1101 24-25 -1.992781475815569 1101 26-27 -2.041456468734019 1101 28-29 -1.1159851681959125 1101 30-31 -1.4989578325208868 1101 32-33 -0.8770461426836533 1101 34-35 -1.9797290501935692 1101 36-37 -0.26574921685795516 1101 38-39 -1.16106784961881 1101 40-41 -0.5904885408699769 1101 42-43 -1.0629696169753515 1101 44-45 -0.4464792402223168 1101 46-47 -0.2937348172533589 1101 48-49 -1.5227299658688835 1101 50-51 -0.8560204984324784 1101 52-53 -1.597912131010311 1101 54-55 -2.278864388254327 1101 56-57 -1.2225758279097505 1101 58-59 -0.12717591346039114 1101 60-61 -0.8327665035061713 1101 62-63 -2.2617756083779845 1101 64-65 -0.9657243059951739 1101 66-67 -0.8400406356235877 1101 68-69 -0.5729205186247412 1101 70-71 -0.4899811091749271 1101 72-73 -1.4811167522685906 1101 74-75 -1.4850005492156697 1101 76 -2.0039448062590175 1102 1 0.7796244886835204 1102 2 -0.5850396526834523 1102 3 -0.2029510653578548 1102 4 0.16426372964017943 1102 5 0.1307870932799844 1102 6 0.21085736349973416 1102 7 1.2110746466304718 1102 8 2.036078966432129 1102 9 1.4194080118819343 1102 10-11 0.23779809033758426 1102 12-13 -4.3072707051460455 1102 14-15 -0.94087408375011 1102 16-17 -2.036164876082786 1102 18-19 -0.3636912356747857 1102 20-21 0.26156282084376414 1102 22-23 -0.6667217655331115 1102 24-25 0.6958609687344506 1102 26-27 -5.418203736869344 1102 28-29 -0.19337758148890316 1102 30-31 0.5123947218038296 1102 32-33 -6.208933317297948 1102 34-35 -0.5660109636240875 1102 36-37 0.11628674123261362 1102 38-39 0.9676236059586927 1102 40-41 0.29346034762993867 1102 42-43 0.30502568196691016 1102 44-45 -6.24164565266512 1102 46-47 -0.012896628660925558 1102 48-49 0.8598936244389392 1102 50-51 -6.466982964987448 1102 52-53 -1.5251707642027768 1102 54-55 1.2047569265414992 1102 56-57 -0.4761703866854674 1102 58-59 1.1948340387788647 1102 60-61 0.6446784216019736 1102 62-63 -4.9224367571364525 1102 64-65 -0.8590878127842032 1102 66-67 0.036331548117750856 1102 68-69 -7.931638730222353 1102 70-71 0.23191881830875616 1102 72-73 0.6733680068021997 1102 74-75 -6.80978612597044 1102 76 -2.09201462196755 1103 1 -0.5361082699371664 1103 2 -0.4597317709100537 1103 3 -0.6583082082149971 1103 4 -0.2853976004090839 1103 5 0.2268462065804755 1103 6 -1.8879726857613477 1103 7 0.5728357303743152 1103 8 0.5622489171710399 1103 9 -0.5414910028963931 1103 10-11 -0.19331336286438727 1103 12-13 -2.3578402864268355 1103 14-15 -1.8418131231589712 1103 16-17 -1.616522018939925 1103 18-19 -1.049036063260985 1103 20-21 -0.7610664895010615 1103 22-23 -1.008085681789268 1103 24-25 -0.3216882923492861 1103 26-27 -0.8892628501698354 1103 28-29 -0.2619582465135366 1103 30-31 -0.42364283977252626 1103 32-33 -5.767353883800411 1103 34-35 -1.759052835545262 1103 36-37 -0.4198123966984184 1103 38-39 0.12602877837247917 1103 40-41 0.15945480575801696 1103 42-43 -0.056658431333588055 1103 44-45 -5.594401194537042 1103 46-47 -0.6417451508284131 1103 48-49 -0.37194135093052694 1103 50-51 -0.7828696644948394 1103 52-53 -1.122692315926919 1103 54-55 -0.07110514242401678 1103 56-57 0.6104213620830024 1103 58-59 -0.5204522912704022 1103 60-61 0.030608224557646224 1103 62-63 -0.46384685565861616 1103 64-65 -0.2841955714048936 1103 66-67 -1.1232281809462918 1103 68-69 -3.1998807252962465 1103 70-71 0.16272238973732556 1103 72-73 1.3408556915312673 1103 74-75 -2.2057220865615754 1103 76 -0.5193545234453829 1104 1 -2.013202729216207 1104 2 -0.261550863032884 1104 3 -0.2877307716328872 1104 4 0.15314545700080018 1104 5 -1.129629093820057 1104 6 -0.17043263235681394 1104 7 0.6501888678910035 1104 8 2.113400452551552 1104 9 -0.5789576250592745 1104 10-11 -0.14243302241045086 1104 12-13 -0.45181975905700966 1104 14-15 0.2252554682962966 1104 16-17 -0.8535881070574192 1104 18-19 0.37235913033047296 1104 20-21 0.6243223623013421 1104 22-23 0.07346054651514322 1104 24-25 0.20426297600852195 1104 26-27 0.19868775063122968 1104 28-29 -0.827107612334629 1104 30-31 -1.043990803724462 1104 32-33 -1.1144625286602263 1104 34-35 -0.12245403714740632 1104 36-37 0.06790038701319645 1104 38-39 -0.04298824432444093 1104 40-41 0.6116201929409257 1104 42-43 0.3221174364901813 1104 44-45 -3.3480518954716203 1104 46-47 0.0015709372890810869 1104 48-49 0.49513141275438954 1104 50-51 0.5867898815665811 1104 52-53 -0.3315332705330576 1104 54-55 -0.3851905896870349 1104 56-57 0.23485811108166388 1104 58-59 0.9544100251514962 1104 60-61 -0.342764605882941 1104 62-63 -0.11959618810188033 1104 64-65 0.2706483872065846 1104 66-67 -0.08586586452439349 1104 68-69 -4.016406926898384 1104 70-71 -0.937974372612473 1104 72-73 0.2253683751093689 1104 74-75 -1.139467079886007 1104 76 0.19860275292310448 1105 1 -1.3419819086303129 1105 2 0.2565959036575336 1105 3 -0.47969674120666994 1105 4 0.13426527595222737 1105 5 0.16887054995011397 1105 6 1.1387329516505673 1105 7 0.9291377803422805 1105 8 1.0523594232568882 1105 9 0.3199359669947057 1105 10-11 1.0028249298966614 1105 12-13 -1.035020379316009 1105 14-15 0.36305354884615326 1105 16-17 0.8107281412138221 1105 18-19 0.7100126234782849 1105 20-21 0.19026679044768713 1105 22-23 0.25153355267581645 1105 24-25 0.6422370760043279 1105 26-27 -0.028356379958431432 1105 28-29 -1.2605869300497332 1105 30-31 0.01144484440428073 1105 32-33 0.43633165431617726 1105 34-35 0.5603337756014355 1105 36-37 0.6523268569851197 1105 38-39 0.2905266963737674 1105 40-41 0.4158509460527 1105 42-43 0.9985245443230468 1105 44-45 1.5162350162252878 1105 46-47 0.8888241957442986 1105 48-49 0.3475893985890153 1105 50-51 -0.3617453851867012 1105 52-53 -0.7115676362344132 1105 54-55 -0.645416481309347 1105 56-57 0.7568919738703173 1105 58-59 0.1834134198122399 1105 60-61 0.48110069733150596 1105 62-63 0.9534937913625186 1105 64-65 0.5535786918878642 1105 66-67 0.6991913417955473 1105 68-69 1.5733315584961929 1105 70-71 1.67234162420241 1105 72-73 0.8495039939015427 1105 74-75 -0.7280635343514525 1105 76 -0.4724294753992595 1106 1 -0.8697651022353057 1106 2 -0.16215585648631148 1106 3 -0.6335928873040224 1106 4 0.5452399082175567 1106 5 0.19406497814707535 1106 6 0.060051813893586825 1106 7 1.4653046744736926 1106 8 1.369055335390506 1106 9 1.621164276606713 1106 10-11 0.9060116026290572 1106 12-13 0.4963155969410096 1106 14-15 0.43910259457739187 1106 16-17 0.7661125566294373 1106 18-19 0.021271045089598317 1106 20-21 -0.1426874349807008 1106 22-23 0.5557622305640777 1106 24-25 1.222954564793568 1106 26-27 0.25419815742160523 1106 28-29 -0.35761689040587186 1106 30-31 -0.06896935806099691 1106 32-33 1.07164068141698 1106 34-35 0.5901966468563842 1106 36-37 0.6380615681049022 1106 38-39 0.4985486196423281 1106 40-41 0.6884295297055658 1106 42-43 1.0336732629383292 1106 44-45 1.4028059310668226 1106 46-47 0.6330780030708283 1106 48-49 0.4698029554876939 1106 50-51 0.6566575956845995 1106 52-53 0.16993406433532954 1106 54-55 0.24911569953594892 1106 56-57 0.930566720951191 1106 58-59 0.35565838850875764 1106 60-61 0.8244099843920125 1106 62-63 1.0537299214290243 1106 64-65 0.7858635210726774 1106 66-67 0.6463003732290034 1106 68-69 1.1684659793276353 1106 70-71 0.7881645049892185 1106 72-73 0.7649412677907534 1106 74-75 -0.6100526593704103 1106 76 0.5622534820756648 1107 1 -0.5551757302546285 1107 2 -0.09858097725925674 1107 3 0.33355687115008337 1107 4 -0.3057745845360671 1107 5 -0.5245823648480936 1107 6 0.34333683804817383 1107 7 -0.2558547458161655 1107 8 0.18316161558374233 1107 9 -1.4485941774995652 1107 10-11 -0.707708204134228 1107 12-13 0.013320427858879214 1107 14-15 -0.5781920914129444 1107 16-17 0.4066922667743569 1107 18-19 0.883106793881872 1107 20-21 0.18938985970528677 1107 22-23 -0.4365083008368842 1107 24-25 -0.7303787685397651 1107 26-27 0.1419276260206388 1107 28-29 -1.5255395957198843 1107 30-31 -0.18152974453442994 1107 32-33 0.5285488939773657 1107 34-35 0.646621767629334 1107 36-37 -0.16908819034921407 1107 38-39 0.2741041751978841 1107 40-41 0.3564488533770671 1107 42-43 0.09531577501562083 1107 44-45 -0.43738730564815853 1107 46-47 -0.19204276987603208 1107 48-49 -0.07840960489878057 1107 50-51 0.8953049386797645 1107 52-53 0.9609485570889547 1107 54-55 0.3781012067823326 1107 56-57 1.0030304890671289 1107 58-59 0.5130496928565833 1107 60-61 -0.01810209290267295 1107 62-63 -0.6974778080395794 1107 64-65 -0.0789673968017226 1107 66-67 1.25721824762514 1107 68-69 1.3160022112116891 1107 70-71 0.13318865957860027 1107 72-73 0.1500620407376161 1107 74-75 0.10086521502572765 1107 76 -1.37475134884221 1108 1 -0.06953008460898147 1108 2 0.19559319691491694 1108 3 -0.19587255827934769 1108 4 0.255907097145613 1108 5 0.011273491007763425 1108 6 0.17018368489502222 1108 7 0.946307416345995 1108 8 0.7135520059741296 1108 9 -0.752317901223293 1108 10-11 -0.13485535128137371 1108 12-13 0.8582753828138365 1108 14-15 0.887333434112584 1108 16-17 -0.46312755304546016 1108 18-19 0.14707075784583168 1108 20-21 0.4992697395851664 1108 22-23 -0.40092271525129775 1108 24-25 -0.3018202099812015 1108 26-27 -0.5741084100153984 1108 28-29 0.21376971358942143 1108 30-31 0.004506291501606086 1108 32-33 0.10131165674012976 1108 34-35 0.42313828414584975 1108 36-37 0.252143040882018 1108 38-39 0.7763263974200996 1108 40-41 0.30743984436805505 1108 42-43 0.46636682606667534 1108 44-45 0.33507515681431244 1108 46-47 1.0728220949888296 1108 48-49 -0.2146858811750576 1108 50-51 0.04290253627736007 1108 52-53 -0.0602526441122464 1108 54-55 0.24131441999554681 1108 56-57 0.3236010596376957 1108 58-59 -1.4712446014377107 1108 60-61 0.7239399491393712 1108 62-63 0.39486453430276924 1108 64-65 0.09034191250758994 1108 66-67 -0.04305202264513497 1108 68-69 1.5111673763768536 1108 70-71 2.001867338257277 1108 72-73 0.9032752539508309 1108 74-75 0.9475919417524494 1108 76 0.6514948974040387 1109 1 -0.4769418631998015 1109 2 -0.5283141165120497 1109 3 0.7427756969627985 1109 4 -0.24576488050889367 1109 5 0.5571643600427336 1109 6 0.5846177696347823 1109 7 -0.8606873513474582 1109 8 0.6344182871024202 1109 9 0.41269645811421185 1109 10-11 1.5656639453077972 1109 12-13 0.5921850566798454 1109 14-15 0.26331203279860915 1109 16-17 -0.12474878126057831 1109 18-19 0.7791766628775036 1109 20-21 1.3601710338925699 1109 22-23 1.1950783076056197 1109 24-25 1.218587752566492 1109 26-27 1.0281576114646 1109 28-29 0.9868233348963216 1109 30-31 0.7308428901089457 1109 32-33 1.5487623825751342 1109 34-35 0.6945887739369567 1109 36-37 0.6656231148424467 1109 38-39 0.9757441557898261 1109 40-41 0.8477443410044359 1109 42-43 0.5720358138317323 1109 44-45 0.4083962945459234 1109 46-47 -0.20325092125884936 1109 48-49 0.1661512878717275 1109 50-51 0.9679881021926207 1109 52-53 1.0873338069676564 1109 54-55 0.6531618569521527 1109 56-57 0.28808628722336493 1109 58-59 0.3450050543315939 1109 60-61 1.4616164903093605 1109 62-63 -0.25570197106723214 1109 64-65 0.8943271204229291 1109 66-67 0.22394313845483538 1109 68-69 1.635788722613924 1109 70-71 0.4521309206169306 1109 72-73 0.3380484550995746 1109 74-75 1.8703072334633788 1109 76 -0.05787604559136028 1110 1 -0.3749594077374212 1110 2 -0.12115718178236534 1110 3 -0.42671844841727236 1110 4 0.12978097101948904 1110 5 -0.11449386927287364 1110 6 0.45755512713371616 1110 7 0.8387175255702672 1110 8 1.6658361485237592 1110 9 -1.652979624893863 1110 10-11 -1.1246305246848678 1110 12-13 -0.45508665178713414 1110 14-15 -0.14876239623103515 1110 16-17 -0.2804758748185634 1110 18-19 0.45080590892612094 1110 20-21 -0.9570604844441704 1110 22-23 -0.16423691440621013 1110 24-25 0.34021120196170784 1110 26-27 -1.8758304860737596 1110 28-29 0.1623266776331107 1110 30-31 -0.6959840218205642 1110 32-33 0.5386472223942818 1110 34-35 0.019945268120977744 1110 36-37 -0.6639456141446871 1110 38-39 0.58901076320182 1110 40-41 0.5605491483623197 1110 42-43 0.7216087936980244 1110 44-45 1.158502566524902 1110 46-47 0.3266887935457987 1110 48-49 0.619604161079593 1110 50-51 0.2046461382864493 1110 52-53 -0.2725224360280549 1110 54-55 -0.6656057745352726 1110 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50-51 0.23141604979087305 2116 52-53 1.0390041126445126 2116 54-55 0.3278234290045532 2116 56-57 -0.23752506648843053 2116 58-59 -0.17000586269897155 2116 60-61 -1.5542132040137844 2116 62-63 0.7316888586270949 2116 64-65 1.059643714309395 2116 66-67 -0.8252817523748561 2116 68-69 0.9837799889894683 2116 70-71 -3.4623668959769596 2116 72-73 0.9647842629598316 2116 74-75 -2.1310792294187166 2116 76 -0.9936402377310998 2117 1 0.25100784462459913 2117 2 0.1421919696006455 2117 3 0.5065037310534635 2117 4 0.023500777782775373 2117 5 -0.9073842972152448 2117 6 -1.2732815194397489 2117 7 -2.3849368714200274 2117 8 -2.51500263562399 2117 9 -0.016516882813576217 2117 10-11 -0.5311864650037919 2117 12-13 0.4794073843806146 2117 14-15 -0.7613804972100411 2117 16-17 0.17190965807870384 2117 18-19 -0.3772796795480815 2117 20-21 -0.45669709681645543 2117 22-23 -0.31621844576442015 2117 24-25 -0.37849471056874506 2117 26-27 0.47400492070662636 2117 28-29 -0.10882461987447556 2117 30-31 -0.4868437541962649 2117 32-33 1.032993338421818 2117 34-35 -0.4242961067668034 2117 36-37 -1.1324698328612897 2117 38-39 0.06618146988387252 2117 40-41 -1.3067395524200336 2117 42-43 -0.9518339834384832 2117 44-45 0.27237114845812727 2117 46-47 -0.7075017070740941 2117 48-49 -0.8925158850920099 2117 50-51 0.1373339241870113 2117 52-53 0.9211417938039332 2117 54-55 -1.1083722231693542 2117 56-57 -0.7071144384691053 2117 58-59 -1.076708761249698 2117 60-61 1.056294042363028 2117 62-63 -0.4897483394405384 2117 64-65 -0.6320108750625835 2117 66-67 -1.2643276460946637 2117 68-69 -0.20593015593806285 2117 70-71 0.4765703020906713 2117 72-73 0.624844649433264 2117 74-75 -0.7815502439114681 2117 76 -1.6696305758953507 2118 1 0.18827626543037823 2118 2 0.362303596635023 2118 3 -0.4061410792274778 2118 4 -0.17118990276691903 2118 5 -0.21836877261508292 2118 6 -0.40643662473500086 2118 7 0.5689348981967797 2118 8 -4.023656075894145 2118 9 -1.6979577157951447 2118 10-11 -0.6700167262485692 2118 12-13 -0.28357277602462005 2118 14-15 -0.6508673880688178 2118 16-17 -0.48554074293437566 2118 18-19 -1.4867961187394911 2118 20-21 -3.1826597627326834 2118 22-23 -0.4468642878919056 2118 24-25 -0.16539494976953506 2118 26-27 0.4902447781242003 2118 28-29 -0.7595093907576427 2118 30-31 -0.46275951799721327 2118 32-33 -0.1358308255479841 2118 34-35 0.8745289952452993 2118 36-37 -0.20346790987456131 2118 38-39 -0.18322052814624357 2118 40-41 -1.7280495284999589 2118 42-43 -0.5202289930534221 2118 44-45 0.07595681517169339 2118 46-47 -0.8179327375135728 2118 48-49 -0.8806965520400922 2118 50-51 -0.3319334647722272 2118 52-53 -1.6481560815302458 2118 54-55 0.09498362317930997 2118 56-57 0.6028147393368144 2118 58-59 0.4762320013786976 2118 60-61 -1.7908636894506778 2118 62-63 0.05171313047175374 2118 64-65 -0.9937786157876971 2118 66-67 -2.0543351504331113 2118 68-69 0.9101877559797629 2118 70-71 -0.7687746629672461 2118 72-73 -2.3619875817003617 2118 74-75 0.5385566929113779 2118 76 0.45810733508443846 2119 1 0.774749428064311 2119 2 0.4883159715029741 2119 3 0.6460496748345967 2119 4 -0.041203484939060786 2119 5 0.7409098631311792 2119 6 1.3380173216405709 2119 7 -1.2970291845035575 2119 8 0.9981760082147133 2119 9 0.2825745041354395 2119 10-11 -0.3026189698221984 2119 12-13 0.5393307905531799 2119 14-15 -0.09961984040545957 2119 16-17 -0.8803543653499943 2119 18-19 -0.9257014962735681 2119 20-21 -0.8902532030465835 2119 22-23 1.1356851361233922 2119 24-25 0.17114109329097715 2119 26-27 0.545692738283158 2119 28-29 0.8752433634050405 2119 30-31 0.7150160240320673 2119 32-33 0.26672966542238896 2119 34-35 0.17835464039848148 2119 36-37 -0.6363996468834614 2119 38-39 0.12952156149609806 2119 40-41 0.17741603817844265 2119 42-43 -0.6759335053528304 2119 44-45 -1.6056544328790103 2119 46-47 0.007611806980619917 2119 48-49 -0.25994098083430117 2119 50-51 1.1095248580810306 2119 52-53 -1.3268119495316526 2119 54-55 -1.828692115036901 2119 56-57 0.7738998039778906 2119 58-59 1.3750070906689018 2119 60-61 0.5267453452090152 2119 62-63 0.45105414360118345 2119 64-65 0.6071696210451449 2119 66-67 0.9772182476251388 2119 68-69 -0.7091215654146765 2119 70-71 -0.5626777768059767 2119 72-73 -2.6110317992163274 2119 74-75 -0.20026316724255366 2119 76 1.6692095032015466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 9.0 13 85.0 14 225.0 15 413.0 16 670.0 17 658.0 18 703.0 19 645.0 20 635.0 21 630.0 22 602.0 23 657.0 24 741.0 25 824.0 26 986.0 27 1246.0 28 1476.0 29 1803.0 30 2260.0 31 2857.0 32 3599.0 33 4833.0 34 6690.0 35 9917.0 36 16898.0 37 19608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.166196755717955 22.6050345795456 11.229818062281112 30.998950602455338 2 17.354687284274473 19.95657249005309 38.63542228860467 24.053317937067764 3 17.608223614022318 25.42643053481104 29.74156866190554 27.223777189261106 4 12.501098239052125 18.61985867232312 40.578364063107955 28.3006790255168 5 11.904911325041105 36.672398428576805 37.946355729042466 13.476334517339625 6 26.534710629698893 40.43025868236416 18.011120454859235 15.023910233077705 7 25.279580284412535 31.83763633853376 23.715687874185733 19.167095502867973 8 22.357636840585897 35.23025366184278 19.66663738029194 22.74547211727938 9 23.43704893753216 15.913797647885733 22.70405281588493 37.945100598697174 10-11 24.37023834925257 25.843761374618758 27.701981850815205 22.084018425313467 12-13 23.222421648488194 24.00938837498274 26.52592471728189 26.242265259247173 14-15 21.30709274158121 25.56323974244725 24.402244173057372 28.72742334291416 16-17 22.49319091787682 27.366862048623748 26.232851781657523 23.907095251841902 18-19 21.52172003062518 26.663989055263386 26.93195938398203 24.882331530129402 20-21 21.506658466481742 27.641108029068818 26.60123253799907 24.251000966450363 22-23 22.710956032784004 26.72925583321828 27.006639639526565 23.553148494471152 24-25 21.98298043251792 27.906568097096883 26.45249959208264 23.657951878302562 26-27 20.86465929486777 28.113037038896486 27.350545354135026 23.671758312100714 28-29 21.98800095389906 26.913132428802733 28.60128274321288 22.497583874085326 30-31 21.931520088361175 27.3806684824219 27.33862161585481 23.349189813362116 32-33 20.747304607583498 28.259259724122348 27.50304369108732 23.490391977206833 34-35 22.34696823265096 27.33548378999159 27.634204812169745 22.683343165187704 36-37 22.898598019404314 26.790757220137312 26.877361213962068 23.433283546496302 38-39 21.957250260439544 27.171061714759077 27.774779410841816 23.09690861395956 40-41 21.838012877637343 26.44873420104678 28.18771729444103 23.525535626874852 42-43 21.30583761123593 26.45124446173735 28.126215907521996 24.116702019504725 44-45 20.68266539480125 26.31129742823792 28.480790230065395 24.525246946895436 46-47 21.084307105292886 26.18390169819136 28.789552295005837 23.94223890150992 48-49 20.040038658014637 26.30125638547563 29.620448583585407 24.038256372924327 50-51 20.424108543672258 26.470071416916646 28.60316543873081 24.50265460068028 52-53 19.984812922822034 26.702898095967264 29.009827670603595 24.302461310607107 54-55 19.15454419941511 27.10579493680419 29.018613583020596 24.721047280760107 56-57 19.711822072722253 26.649555056292595 28.69290725841879 24.945715612566367 58-59 19.841728063459392 26.662733924918104 28.43686066798037 25.058677343642138 60-61 20.231446035670807 26.391625770336248 27.75971784669838 25.617210347294566 62-63 19.816625456553663 26.30439421133885 28.12935373338521 25.749626598722276 64-65 20.71216095791548 26.39413603102682 27.498023169706176 25.395679841351527 66-67 20.658190353068164 26.564833757985767 26.73929687598057 26.037679012965498 68-69 20.09840221907045 26.515256109346957 27.07504424334467 26.31129742823792 70-71 21.116312929097685 26.493291328304448 26.248540910973606 26.14185483162426 72-73 21.18597266326108 26.718587225283347 25.672436082487167 26.42300402896841 74-75 20.824495123818608 27.586509859048864 25.66176747455223 25.927227542580294 76 21.66354975964254 26.410452725515544 26.072822662633516 25.8531748522084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 1.5 14 1.5 15 1.0 16 1.0 17 0.0 18 0.5 19 3.5 20 13.0 21 20.0 22 20.0 23 27.5 24 49.0 25 63.0 26 116.0 27 195.5 28 303.5 29 385.0 30 462.0 31 591.5 32 886.5 33 1129.0 34 1303.0 35 1726.5 36 2176.5 37 2377.0 38 2792.0 39 3553.0 40 4326.5 41 5011.0 42 5268.0 43 5407.0 44 5899.0 45 6168.0 46 6084.0 47 6147.5 48 5982.0 49 5408.0 50 5063.0 51 4695.0 52 3857.5 53 3099.5 54 2811.0 55 2605.5 56 2039.5 57 1470.5 58 1262.0 59 1056.0 60 703.0 61 466.0 62 376.0 63 302.5 64 206.5 65 154.0 66 118.5 67 113.0 68 88.0 69 59.5 70 41.5 71 27.0 72 26.0 73 23.0 74 18.5 75 16.0 76 13.5 77 9.5 78 10.0 79 12.0 80 7.5 81 2.5 82 2.5 83 3.0 84 2.5 85 1.5 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 1.0 95 2.0 96 2.0 97 1.5 98 1.5 99 4.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7279756002660875 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 79673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.114267066634866 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06613520868957 38.599023508591365 2 9.785409792635052 10.199189185796945 3 5.062498494737603 7.914851957375774 4 3.082777389754582 6.426267367866152 5 1.953228486789817 5.0895535501361815 6 1.416150863418511 4.428099858170271 7 1.045254208713663 3.8130859889799558 8 0.7562438284241708 3.1528874273593313 9 0.5298523638640688 2.4851580836669886 >10 2.295224103465716 17.657173697488485 >50 0.007225259507237302 0.23470937456854893 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002510260690572716 0.0 2 0.0 0.0 0.0 0.002510260690572716 0.0 3 0.0 0.0 0.0 0.003765391035859074 0.0 4 0.0 0.0 0.0 0.00627565172643179 0.0 5 0.0 0.0 0.0 0.00627565172643179 0.0 6 0.0 0.0 0.0 0.00627565172643179 0.0 7 0.0 0.0 0.0 0.00627565172643179 0.0 8 0.0 0.0 0.0 0.008785912417004505 0.0 9 0.0 0.0 0.0 0.013806433798149938 0.0 10 0.0 0.0 0.0 0.01882695517929537 0.0 11 0.0 0.0 0.0 0.021337215869868087 0.0 12 0.0 0.0 0.0 0.021337215869868087 0.0 13 0.0 0.0 0.0 0.022592346215154442 0.0 14 0.0 0.0 0.0 0.0238474765604408 0.0 15 0.0 0.0 0.0 0.02510260690572716 0.0 16 0.0 0.0 0.0 0.026357737251013518 0.0 17 0.0 0.0 0.0 0.03514364966801802 0.0 18 0.0 0.0 0.0 0.042674431739736174 0.0 19 0.0 0.0 0.0 0.0476949531208816 0.0 20 0.0 0.0 0.0 0.05020521381145432 0.0 21 0.0 0.0 0.0 0.05397060484731339 0.0 22 0.0 0.0 0.0 0.0715424296813224 0.0 23 0.0 0.0 0.0 0.07530782071718148 0.0 24 0.0 0.0 0.0 0.08158347244361328 0.0 25 0.0 0.0 0.0 0.08158347244361328 0.0 26 0.0 0.0 0.0 0.08534886347947235 0.0 27 0.0 0.0 0.0 0.09413477589647685 0.0 28 0.0 0.0 0.0 0.12300277383806309 0.0 29 0.0 0.0 0.0 0.14182972901735846 0.0 30 0.0 0.0 0.0 0.16818746626837197 0.0 31 0.0 0.0 0.0 0.20207598559110362 0.0 32 0.0 0.0 0.0 0.22843372284211716 0.0 33 0.0 0.0 0.0 0.2824043276894305 0.0 34 0.0 0.0 0.0 0.34265058426317574 0.0 35 0.0 0.0 0.0 0.4279994477426481 0.0 36 0.0 0.0 0.0 0.5899112622845882 0.0 37 0.0 0.0 0.0 0.8585091561758689 0.0 38 0.0 0.0 0.0 1.1999046100937583 0.0 39 0.0 0.0 0.0 1.6555169254327062 0.0 40 0.0 0.0 0.0 2.26425514289659 0.0 41 0.0 0.0 0.0 2.9294742258983595 0.0 42 0.0 0.0 0.0 3.681297302724888 0.0 43 0.0 0.0 0.0 4.392956208502253 0.0 44 0.0 0.0 0.0 5.163606240508077 0.0 45 0.0 0.0 0.0 5.860203582142005 0.0 46 0.0 0.0 0.0 6.639639526564833 0.0 47 0.0 0.0 0.0 7.281011133006163 0.0 48 0.0 0.0 0.0 7.9876495174023825 0.0 49 0.0 0.0 0.0 8.741982854919483 0.0 50 0.0 0.0 0.0 9.38084420067024 0.0 51 0.0 0.0 0.0 10.143963450604346 0.0 52 0.0 0.0 0.0 10.80039662118911 0.0 53 0.0 0.0 0.0 11.515820918002335 0.0 54 0.0 0.0 0.0 12.20990799894569 0.0 55 0.0 0.0 0.0 12.78601282743213 0.0 56 0.0 0.0 0.0 13.335759918667554 0.0 57 0.0 0.0 0.0 13.938222484405005 0.0 58 0.0 0.0 0.0 14.46412209907999 0.0 59 0.0 0.0 0.0 15.062819273781582 0.0 60 0.0 0.0 0.0 15.58746375811128 0.0 61 0.0 0.0 0.0 16.199967366611023 0.0 62 0.0 0.0 0.0 16.796154280622044 0.0 63 0.0 0.0 0.0 17.333350068404602 0.0 64 0.0 0.0 0.0 17.964680632083642 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGTGG 15 0.0022179203 69.99498 8 TCGTGAC 15 0.0022179203 69.99498 41 GTCGCCC 20 0.006930391 52.49623 37 ATCTACG 20 0.006930391 52.49623 52 CACGGTT 20 0.006930391 52.49623 49 CTATAAC 20 0.006930391 52.49623 4 GCAAAAA 20 0.006930391 52.49623 13 TTCCGAC 20 0.006930391 52.49623 42 CTGACTT 20 0.006930391 52.49623 21 TTTCCGA 20 0.006930391 52.49623 41 ACTAAAT 20 0.006930391 52.49623 67 CTAATTC 20 0.006930391 52.49623 30 CTTACTC 20 0.006930391 52.49623 3 CCATAGG 30 5.704872E-4 46.898846 1 GGGGCAC 30 5.8483955E-4 46.66332 37 CCGATGA 35 0.0012491874 39.99713 9 CACTGGT 35 0.0012491874 39.99713 59 CTGGTTA 35 0.0012491874 39.99713 61 GCGGGGA 35 0.0012491874 39.99713 18 GGGCACC 35 0.0012491874 39.99713 38 >>END_MODULE