FastQCFastQC Report
Wed 25 May 2016
SRR1780684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79673
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7650.960174714144064No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT3680.46188796706537977Illumina PCR Primer Index 9 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1740.2183926800798263No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC990.12425790418334944No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG150.002217289370.034
ATCAGTA150.002217289370.021
GATCAGT150.002217289370.020
GCGGGTA150.002217289370.033
TGGATTC150.002217289370.026
ACATCCA150.002217289370.036
ATTGGCG452.6609996E-854.44444721
ATAAAGA452.6609996E-854.4444471
ATTTAAT200.00692842652.49999634
TCGTTTT200.00692842652.49999654
TGCCCGT200.00692842652.49999659
CTCAAGC200.00692842652.49999612
GATCGGA200.00692842652.49999639
GTCGAAA200.00692842652.49999643
CGAAATC200.00692842652.49999645
GCGAATA200.00692842652.49999657
GAGTCGA200.00692842652.49999641
GGCACGG200.00692842652.49999612
AGGACGC200.00692842652.49999612
GGATCGG200.00692842652.49999638
Produced by FastQC (version 0.11.5)