##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780684_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 79673 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.8062831825085 34.0 34.0 34.0 31.0 34.0 2 33.30431890351813 34.0 34.0 34.0 33.0 34.0 3 33.455813136194195 34.0 34.0 34.0 34.0 34.0 4 33.51889598734829 34.0 34.0 34.0 34.0 34.0 5 33.5351624766232 34.0 34.0 34.0 34.0 34.0 6 37.253147239340805 38.0 38.0 38.0 36.0 38.0 7 36.92958718762944 38.0 37.0 38.0 35.0 38.0 8 36.92864583987047 38.0 37.0 38.0 35.0 38.0 9 36.71041632673553 38.0 37.0 38.0 34.0 38.0 10-11 36.411764336726364 38.0 37.0 38.0 34.0 38.0 12-13 36.32211665181429 38.0 37.0 38.0 34.0 38.0 14-15 36.59071454570557 38.0 37.0 38.0 34.0 38.0 16-17 36.708922721624646 38.0 37.0 38.0 34.0 38.0 18-19 36.736529313569214 38.0 37.0 38.0 34.0 38.0 20-21 36.47689932599501 38.0 37.0 38.0 34.0 38.0 22-23 36.53242001681875 38.0 37.0 38.0 34.0 38.0 24-25 35.89732406210385 38.0 36.5 38.0 32.5 38.0 26-27 36.11466870834536 38.0 37.0 38.0 33.0 38.0 28-29 36.00380932059794 38.0 37.0 38.0 33.0 38.0 30-31 35.72218944937432 38.0 36.5 38.0 31.5 38.0 32-33 36.221097485973914 38.0 37.0 38.0 34.0 38.0 34-35 36.26640141578703 38.0 37.0 38.0 34.0 38.0 36-37 36.379143499052375 38.0 37.0 38.0 34.0 38.0 38-39 36.04127496140474 38.0 37.0 38.0 32.5 38.0 40-41 35.57864019178392 38.0 36.0 38.0 31.0 38.0 42-43 35.97367991665935 38.0 36.5 38.0 32.5 38.0 44-45 36.14478556098051 38.0 37.0 38.0 34.0 38.0 46-47 36.441197143323336 38.0 37.0 38.0 34.0 38.0 48-49 36.24382162087532 38.0 37.0 38.0 34.0 38.0 50-51 36.38775996887277 38.0 37.0 38.0 34.0 38.0 52-53 36.311316255193105 38.0 37.0 38.0 34.0 38.0 54-55 36.481932398679604 38.0 37.0 38.0 34.0 38.0 56-57 36.5247637217125 38.0 37.0 38.0 34.0 38.0 58-59 36.58531748522084 38.0 37.0 38.0 34.0 38.0 60-61 36.25360536191683 38.0 37.0 38.0 33.5 38.0 62-63 36.515023910233076 38.0 37.0 38.0 34.0 38.0 64-65 36.53996962584564 38.0 37.0 38.0 34.0 38.0 66-67 35.96237746790004 38.0 37.0 38.0 33.0 38.0 68-69 35.43690459754245 38.0 36.0 38.0 30.0 38.0 70-71 35.45686744568424 38.0 36.0 38.0 31.0 38.0 72-73 35.22129830682916 38.0 36.0 38.0 29.0 38.0 74-75 35.81941812157193 38.0 37.0 38.0 32.5 38.0 76 35.26374053945502 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3724543408954446 1101 2 0.4162559411800686 1101 3 0.2920633887220845 1101 4 0.35208979936395224 1101 5 0.3283892489408373 1101 6 -1.2133589776685838 1101 7 0.24746296001183765 1101 8 0.5867839392831868 1101 9 0.7429846183856981 1101 10-11 0.38309951155212474 1101 12-13 0.6354846625338837 1101 14-15 0.41023712372194154 1101 16-17 0.1401696156934733 1101 18-19 -0.04303205278220901 1101 20-21 0.16550870078464186 1101 22-23 0.6005777893296909 1101 24-25 -1.503946511166589 1101 26-27 -0.31470288882191966 1101 28-29 0.6241038825047056 1101 30-31 0.9664830044764372 1101 32-33 0.27633513143147326 1101 34-35 0.3977524718949397 1101 36-37 0.19663230745397442 1101 38-39 0.18710657829152666 1101 40-41 1.3175191975301743 1101 42-43 0.39733582258374156 1101 44-45 0.43147582462483314 1101 46-47 0.7936441397717928 1101 48-49 -1.2866386393620957 1101 50-51 -1.6107223766110081 1101 52-53 -1.5207945318103882 1101 54-55 -0.8663970474084834 1101 56-57 -1.163686042347777 1101 58-59 -1.2772075425409852 1101 60-61 -1.3578216908450713 1101 62-63 -0.5471478138124937 1101 64-65 -0.8778545288781743 1101 66-67 -1.0434720525484025 1101 68-69 -1.51689838589445 1101 70-71 -1.1309942493370215 1101 72-73 -1.640482853950374 1101 74-75 -1.5052038286152296 1101 76 -0.9273359033230122 1102 1 -0.35275883206846714 1102 2 0.0931082329457169 1102 3 0.3879349598255786 1102 4 0.35046443365666846 1102 5 -0.031341690520541476 1102 6 0.20818586992212573 1102 7 0.2957988355838381 1102 8 0.36951005265869696 1102 9 0.2523992366748118 1102 10-11 0.43589889141259874 1102 12-13 0.5891367342500189 1102 14-15 -0.10356598197684264 1102 16-17 0.21267342905148467 1102 18-19 -0.3773572759574222 1102 20-21 0.26864440631837994 1102 22-23 0.4965918925029058 1102 24-25 -5.158643818060636 1102 26-27 -1.6000795985816225 1102 28-29 0.030645354335867125 1102 30-31 1.483699378160516 1102 32-33 0.5148763031951162 1102 34-35 0.7650976245542864 1102 36-37 -2.3489029379762485 1102 38-39 -0.40263861437681925 1102 40-41 0.775159666635723 1102 42-43 0.9231166232638941 1102 44-45 0.8338413568695842 1102 46-47 0.8533200668553818 1102 48-49 -2.9887466136563106 1102 50-51 -0.4583505929596896 1102 52-53 -0.5250204826493245 1102 54-55 0.19597948731183124 1102 56-57 0.5796562096589213 1102 58-59 -0.5427422878586867 1102 60-61 -5.831528274826464 1102 62-63 -2.6133377065383527 1102 64-65 0.13070989811789957 1102 66-67 0.9021407103232377 1102 68-69 -0.5768243692407111 1102 70-71 -1.5595406722821892 1102 72-73 -6.268724208255094 1102 74-75 -2.802833295245229 1102 76 -0.46298142356506133 1103 1 0.7631586556162588 1103 2 0.4577941935368486 1103 3 -0.00892464608574528 1103 4 0.3610863548389389 1103 5 0.031004368592753906 1103 6 0.1753964610551293 1103 7 0.7503862740074823 1103 8 0.5199102989641133 1103 9 -0.014380319975430211 1103 10-11 0.2111451968313176 1103 12-13 -1.0680369110701804 1103 14-15 -0.44516080956304904 1103 16-17 -0.07865969902733383 1103 18-19 0.4350657535992326 1103 20-21 0.020260785628728684 1103 22-23 0.4097692324043791 1103 24-25 -3.0265625372724543 1103 26-27 -0.7787279975963983 1103 28-29 -0.6353798919695492 1103 30-31 0.6025417426925372 1103 32-33 0.8819483475300913 1103 34-35 0.06659085115526864 1103 36-37 -1.1229946867447254 1103 38-39 -0.3619212498447979 1103 40-41 -0.6811211215416151 1103 42-43 -0.1984197067853657 1103 44-45 0.9518370465247585 1103 46-47 0.589465387052428 1103 48-49 -2.824029864887841 1103 50-51 -0.5596436964079601 1103 52-53 0.04679294099599218 1103 54-55 -0.45241952746648906 1103 56-57 0.3119530076884871 1103 58-59 -0.24401999721828815 1103 60-61 -4.178049210787059 1103 62-63 -1.408596327228011 1103 64-65 0.11131334639376433 1103 66-67 -0.539823575391047 1103 68-69 0.07911774898589385 1103 70-71 -0.050535130410260365 1103 72-73 -4.9569939126885885 1103 74-75 -2.3272329257870936 1103 76 -4.493461719131567 1104 1 -0.11096517615943924 1104 2 0.35069305869038914 1104 3 0.4235686783468253 1104 4 0.0011481038376004449 1104 5 -0.5231928944246036 1104 6 0.0753881165958461 1104 7 0.2364093047150959 1104 8 0.37275575957826845 1104 9 0.858241509130039 1104 10-11 -0.301705270458406 1104 12-13 0.26753551883636106 1104 14-15 0.5647273746826187 1104 16-17 0.6352713757390234 1104 18-19 0.5903352796339405 1104 20-21 -0.38167095669437145 1104 22-23 0.30279326444710364 1104 24-25 0.304962829883749 1104 26-27 1.0775595524703618 1104 28-29 1.218320875720707 1104 30-31 0.42513853842017113 1104 32-33 -0.07514027062745043 1104 34-35 0.18919181911255123 1104 36-37 0.8849951330149608 1104 38-39 1.1499487434796265 1104 40-41 1.544012389806845 1104 42-43 0.4346952798634973 1104 44-45 0.43191464999605245 1104 46-47 0.7612778036078325 1104 48-49 -0.12150983818556682 1104 50-51 0.6091647148069939 1104 52-53 0.4732991158958626 1104 54-55 0.2907380159246955 1104 56-57 0.12858351503160748 1104 58-59 -0.6271767061635529 1104 60-61 0.3682208159152154 1104 62-63 0.2967608156291348 1104 64-65 0.7522512636167278 1104 66-67 -0.08450815483029572 1104 68-69 1.0903410467162047 1104 70-71 1.0598829269996202 1104 72-73 0.9762921353755019 1104 74-75 -0.11004751190191797 1104 76 -1.127890958116879 1105 1 0.5550588798315061 1105 2 0.25513964517041643 1105 3 0.21284825590015544 1105 4 -0.2895983024320401 1105 5 -0.2759903463335718 1105 6 0.1774584405554478 1105 7 0.6145158383892948 1105 8 -0.7605789707347981 1105 9 0.7106156761808791 1105 10-11 0.5300913851721205 1105 12-13 -0.4883003639849761 1105 14-15 0.22798541080851464 1105 16-17 0.2355919825870174 1105 18-19 0.6979481206716187 1105 20-21 0.3384162148407768 1105 22-23 0.21654373592776466 1105 24-25 0.5218439329389426 1105 26-27 0.43999197357592834 1105 28-29 0.90865237901059 1105 30-31 1.128566726677164 1105 32-33 0.8017613680297728 1105 34-35 -0.46783219496900585 1105 36-37 0.3936913318331179 1105 38-39 0.10289497372983192 1105 40-41 0.051021735601239016 1105 42-43 0.26659670897376486 1105 44-45 0.5710014443466633 1105 46-47 0.724114650345193 1105 48-49 -0.10481942286349266 1105 50-51 0.7623854515741044 1105 52-53 0.33996438558279607 1105 54-55 0.3751749632445893 1105 56-57 0.4322484913528015 1105 58-59 0.40777572667664685 1105 60-61 0.8064358949144221 1105 62-63 -0.35010017091794765 1105 64-65 0.43694595369677103 1105 66-67 0.3765161107076054 1105 68-69 0.07705576948557535 1105 70-71 1.0410027459510403 1105 72-73 0.7908640309372927 1105 74-75 -0.3788124421227792 1105 76 -0.17183295551849653 1106 1 -0.5600599434175564 1106 2 0.3792270782849485 1106 3 0.3714670033276377 1106 4 0.16907893592934187 1106 5 0.10918328508689257 1106 6 0.22765232716968598 1106 7 -1.3273181773382703 1106 8 0.40235077515459494 1106 9 -0.5822072695958624 1106 10-11 0.57165244320813 1106 12-13 1.0589256493162935 1106 14-15 -0.3224684355119791 1106 16-17 0.13307037343643202 1106 18-19 0.45722543268826144 1106 20-21 0.4532425834203053 1106 22-23 0.5256249949992764 1106 24-25 -0.5820749596327062 1106 26-27 0.4394720541634385 1106 28-29 0.6203029065121584 1106 30-31 0.46805243972497124 1106 32-33 0.4520505732029747 1106 34-35 0.7332683011207735 1106 36-37 0.9724482911710837 1106 38-39 0.26183867079011947 1106 40-41 0.741842991639885 1106 42-43 0.557025859122156 1106 44-45 1.0810748829636836 1106 46-47 0.7800709771145407 1106 48-49 1.0094328680314177 1106 50-51 -0.15475239206013214 1106 52-53 0.44009219910503816 1106 54-55 -0.17024216376738366 1106 56-57 0.05852391866156381 1106 58-59 0.7219586949832291 1106 60-61 0.9001297057070801 1106 62-63 0.6143936658706792 1106 64-65 -0.8984778889409526 1106 66-67 0.2855085502128176 1106 68-69 1.6548812065428322 1106 70-71 0.20560056662217363 1106 72-73 0.3189938374839443 1106 74-75 -0.4336964351038688 1106 76 0.8026476666517297 1107 1 0.8360753222829231 1107 2 -0.11913040963775501 1107 3 0.23794043327933423 1107 4 0.04598714848972918 1107 5 0.22860357494196393 1107 6 0.23113058803925668 1107 7 0.662826750197965 1107 8 -0.03320478040096475 1107 9 -0.5640430183881335 1107 10-11 0.568367419053537 1107 12-13 -0.13373135551462667 1107 14-15 0.02989871424648527 1107 16-17 0.6809930787504399 1107 18-19 0.6500756742341451 1107 20-21 0.7671556268985711 1107 22-23 0.07238828002343212 1107 24-25 0.3254612722513528 1107 26-27 -0.12661490235830541 1107 28-29 0.627645901681241 1107 30-31 0.6789703141211021 1107 32-33 0.8947558872126322 1107 34-35 0.18515235909178784 1107 36-37 1.0982453926203561 1107 38-39 0.5818386707901197 1107 40-41 0.5665772911567899 1107 42-43 0.6429195789289182 1107 44-45 0.6826207766834855 1107 46-47 0.8560130061000493 1107 48-49 0.6164860081280352 1107 50-51 0.8389674147031485 1107 52-53 0.5673385759166294 1107 54-55 0.5117868217398609 1107 56-57 0.8949490394345148 1107 58-59 0.6188669075436124 1107 60-61 1.0528833288954829 1107 62-63 0.6512052600735814 1107 64-65 0.7241308067112229 1107 66-67 0.2630930912756142 1107 68-69 0.7411613997795499 1107 70-71 0.4742962187960842 1107 72-73 0.8129068809622026 1107 74-75 -0.5516674495966214 1107 76 0.9255462173763718 1108 1 -0.8531138669062628 1108 2 0.18579451528717073 1108 3 -0.08139890606000932 1108 4 0.33313429563687436 1108 5 0.2430780494164395 1108 6 -0.2889294720208042 1108 7 -0.01807415070293672 1108 8 -0.660712287908467 1108 9 -0.4272262015713153 1108 10-11 0.21677582746195156 1108 12-13 0.812875251091981 1108 14-15 0.6121509664987315 1108 16-17 0.05267476043212582 1108 18-19 0.27626186042033396 1108 20-21 0.720248719991659 1108 22-23 -0.2509450533099056 1108 24-25 1.025641452431529 1108 26-27 -0.31904733479073144 1108 28-29 -1.0041258700905331 1108 30-31 0.09176310691390199 1108 32-33 0.43481594727268913 1108 34-35 0.3735607675001944 1108 36-37 0.7911583055332656 1108 38-39 0.7002771092285514 1108 40-41 0.6703911049706051 1108 42-43 -0.6741074480981055 1108 44-45 0.5088069628696772 1108 46-47 0.7834003934874332 1108 48-49 1.0127622844043103 1108 50-51 0.7111896369253756 1108 52-53 0.02766890624696572 1108 54-55 -0.24247744252440384 1108 56-57 0.20903312351860137 1108 58-59 0.13751555619226963 1108 60-61 0.8651055511177006 1108 62-63 0.7246887435570599 1108 64-65 0.49524191782233373 1108 66-67 0.7605705687531028 1108 68-69 1.2213716099897596 1108 70-71 -0.4274455229456535 1108 72-73 0.13554952360485117 1108 74-75 -0.4668926748218496 1108 76 1.0355762474064036 1109 1 -1.3240411260431308 1109 2 0.07333926527733325 1109 3 0.3479452852929157 1109 4 0.04891776469545306 1109 5 0.014843264413094914 1109 6 -0.20145553520188741 1109 7 -0.09461138662882007 1109 8 0.2611571798125283 1109 9 0.5516872096565066 1109 10-11 0.3154028387527106 1109 12-13 0.4280056653490334 1109 14-15 0.8820966764007778 1109 16-17 0.7187611525010524 1109 18-19 0.748979119163792 1109 20-21 0.890377363919427 1109 22-23 0.3877012348997013 1109 24-25 0.22901294619603618 1109 26-27 0.30698043970865996 1109 28-29 -0.5724802506719868 1109 30-31 0.2181842879202307 1109 32-33 0.8640814573242253 1109 34-35 0.14467686176038796 1109 36-37 0.9529664018391841 1109 38-39 1.056113294759065 1109 40-41 0.7846461897497079 1109 42-43 1.0177667405009743 1109 44-45 0.694770218701926 1109 46-47 0.5539460483125609 1109 48-49 0.7003385069150312 1109 50-51 0.278511325426102 1109 52-53 0.2132289204095983 1109 54-55 -0.3531525280903196 1109 56-57 0.05156233395173615 1109 58-59 0.2090270239919363 1109 60-61 0.7926892483520547 1109 62-63 -0.003457524982216853 1109 64-65 0.7397834025384853 1109 66-67 0.5640246778840634 1109 68-69 0.10225795484990385 1109 70-71 0.8247280480052055 1109 72-73 0.3318200299190224 1109 74-75 0.026527601349521035 1109 76 1.0453327287786038 1110 1 -1.489883379781972 1110 2 -0.6555512552621394 1110 3 -0.20764462080129675 1110 4 -0.3149961356793938 1110 5 0.30940986796064607 1110 6 -0.0631860294632034 1110 7 0.5671925319088729 1110 8 0.5822917781044481 1110 9 0.4796541300877806 1110 10-11 -0.6859786969739972 1110 12-13 0.41619981459353994 1110 14-15 0.6025240829780429 1110 16-17 0.6330383098222256 1110 18-19 -0.21646316549053068 1110 20-21 0.6401841421394749 1110 22-23 -0.2929804515399894 1110 24-25 -0.5911168988400917 1110 26-27 1.0130901123909624 1110 28-29 0.43259182105194327 1110 30-31 -0.510764199153229 1110 32-33 0.12205185574753585 1110 34-35 0.5349655350996443 1110 36-37 1.251155913760968 1110 38-39 0.6831759372601311 1110 40-41 -1.1082702997969918 1110 42-43 -0.047522898947185865 1110 44-45 0.7631320844514349 1110 46-47 0.7020602037401673 1110 48-49 1.2433689973119115 1110 50-51 -0.05134723623096704 1110 52-53 0.18062274504151077 1110 54-55 -0.2626281241795141 1110 56-57 0.09889200895270278 1110 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38-39 -3.4584391069876546 2116 40-41 -1.0464782643987576 2116 42-43 -1.1845804210710824 2116 44-45 -0.5093236677609596 2116 46-47 -0.39982032723328587 2116 48-49 0.42954156368359264 2116 50-51 -0.37714369640796264 2116 52-53 0.24956079813885168 2116 54-55 -0.5334909560379231 2116 56-57 -0.1664398494543775 2116 58-59 -2.464466425789716 2116 60-61 -1.2432277822156337 2116 62-63 0.3942608156291314 2116 64-65 -1.4522580821776714 2116 66-67 -0.5160735753910473 2116 68-69 -1.0985608224426784 2116 70-71 0.6690184410183093 2116 72-73 0.27318465873997866 2116 74-75 0.2774992170700443 2116 76 -0.41167600484585165 2117 1 0.8650608295293054 2117 2 0.5241546145168314 2117 3 0.4052834284484135 2117 4 0.038644153320646524 2117 5 0.2637726570675696 2117 6 0.41122720639674526 2117 7 0.47461418981148995 2117 8 -0.0034463262946857753 2117 9 -0.31167586862967767 2117 10-11 -0.5974296823957346 2117 12-13 -1.0135381187996444 2117 14-15 0.05434315869092643 2117 16-17 -1.113306438157771 2117 18-19 -1.1200692533020842 2117 20-21 0.06193823559421929 2117 22-23 0.21886170997511556 2117 24-25 -0.8912537035940602 2117 26-27 -1.9953105545322174 2117 28-29 -1.2613396055651336 2117 30-31 -0.19136785013010638 2117 32-33 -2.115582277038577 2117 34-35 0.24051467793236014 2117 36-37 -0.9719961532733592 2117 38-39 -0.04250915529684107 2117 40-41 -0.014195655703112209 2117 42-43 0.12224325042650719 2117 44-45 -0.20394927162569587 2117 46-47 -0.971136752354063 2117 48-49 -1.1282482913888785 2117 50-51 -0.46876205389588677 2117 52-53 0.28550282712436115 2117 54-55 -0.4528508594195557 2117 56-57 0.08750942590794608 2117 58-59 -0.5268335755481743 2117 60-61 -0.5515611155489566 2117 62-63 -0.2769106819554068 2117 64-65 -1.048236343047229 2117 66-67 -0.547824783120511 2117 68-69 0.03652371862012416 2117 70-71 -0.22676658313627485 2117 72-73 0.9977377988365959 2117 74-75 0.9890088789057927 2117 76 0.7640003236565676 2118 1 0.004360111926935417 2118 2 0.339078867600648 2118 3 -1.007971109331244 2118 4 -0.13817682130530784 2118 5 0.35004909813505947 2118 6 0.419458527629331 2118 7 0.7096875916219076 2118 8 0.46222132531640625 2118 9 -1.4700408608908262 2118 10-11 -0.855602375984212 2118 12-13 -0.3250150664099891 2118 14-15 0.6349796203953417 2118 16-17 -2.433483728153547 2118 18-19 -0.5930419093688712 2118 20-21 -0.18325429758903056 2118 22-23 0.6325500923211678 2118 24-25 0.7491829550992435 2118 26-27 -1.6673592389278795 2118 28-29 0.06241397072115262 2118 30-31 -1.0893792004419964 2118 32-33 0.042770989693757144 2118 34-35 -0.13813782429548382 2118 36-37 -2.848831198533908 2118 38-39 0.12241040757544397 2118 40-41 -0.28392972070943756 2118 42-43 0.4669972488318308 2118 44-45 -2.146095512421148 2118 46-47 0.28598064364049947 2118 48-49 0.6658279714505753 2118 50-51 -0.7046679682526218 2118 52-53 -0.99548774555047 2118 54-55 -0.2074472667175371 2118 56-57 0.5782931602543613 2118 58-59 -0.43427225103243927 2118 60-61 -0.24978118027387808 2118 62-63 -0.4508119999048503 2118 64-65 0.24281473335631887 2118 66-67 0.15768856053128388 2118 68-69 -0.6964977156465579 2118 70-71 -0.07998641335062473 2118 72-73 0.945441940293378 2118 74-75 0.04878562483703064 2118 76 -0.01764687863225589 2119 1 0.43365736027946866 2119 2 -0.6300342668628574 2119 3 0.06421562038353557 2119 4 -0.5414164208827472 2119 5 -0.34337109402752475 2119 6 -0.5020415478271829 2119 7 -0.9142371530835618 2119 8 0.01202262316841285 2119 9 -0.465689535371439 2119 10-11 -0.5822485855521009 2119 12-13 -0.08762887998209834 2119 14-15 -0.48422909231656064 2119 16-17 -0.5997898803744803 2119 18-19 -0.06075909836228277 2119 20-21 -1.2936849027502575 2119 22-23 0.19076479124967705 2119 24-25 0.7150640356365017 2119 26-27 0.3094127314931683 2119 28-29 0.07910243593570243 2119 30-31 0.7937889669708511 2119 32-33 0.5615722372414211 2119 34-35 0.19629801251723222 2119 36-37 -0.04345869488683718 2119 38-39 -0.9252655318581304 2119 40-41 -0.8816207514453964 2119 42-43 0.06665014887710186 2119 44-45 0.25641467420795294 2119 46-47 0.10153977639365763 2119 48-49 -0.049927348233502755 2119 50-51 0.7858873916749403 2119 52-53 -0.05841847647254639 2119 54-55 -0.0034002824627918926 2119 56-57 -1.3196393313196637 2119 58-59 -0.15405191801770002 2119 60-61 0.14187584472737313 2119 62-63 -0.06204747452630244 2119 64-65 -0.06434357440564753 2119 66-67 0.4661673572514431 2119 68-69 0.45085627600292355 2119 70-71 0.10128528039654583 2119 72-73 0.8587675602943818 2119 74-75 -0.40531166375897243 2119 76 0.1719250314235765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 3.0 23 14.0 24 30.0 25 54.0 26 109.0 27 186.0 28 342.0 29 566.0 30 984.0 31 1596.0 32 2589.0 33 4238.0 34 6396.0 35 10411.0 36 18508.0 37 33644.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.000903693848606 24.26920035645702 12.025403838188597 27.70449211150578 2 17.594417180224166 19.639024512695645 38.47476560440802 24.291792702672172 3 16.97563791999799 25.851919721863116 30.243620800020082 26.92882155811881 4 12.100711658905777 19.6704027713278 40.21688652366549 28.01199904610094 5 11.729193076701016 38.1270944987637 37.57985766821885 12.563854756316442 6 26.74682765805229 40.45285102857932 18.303565825310958 14.496755488057433 7 26.33012438341722 30.007656295106244 24.182596362632257 19.479622958844274 8 22.726645162100084 36.73138955480527 20.547738882682967 19.994226400411684 9 23.47595797823604 14.98374606202854 23.18978825951075 38.35050770022467 10-11 24.47315903756605 26.339537861006868 27.867031491220363 21.32027161020672 12-13 23.9259222070212 23.41320146097172 27.193654060974232 25.467222271032846 14-15 21.741995406222937 24.99592082637782 23.831159865952078 29.430923901447166 16-17 22.61117317033374 28.469494056957817 25.4942075734565 23.42512519925194 18-19 22.278563628832853 26.93195938398203 27.093871198523967 23.695605788661155 20-21 21.364201172291743 27.632322116651814 26.12742083265347 24.876055878402973 22-23 22.045109384609592 27.498023169706176 27.26896188169141 23.18790556399282 24-25 22.428551705094574 28.245453290324203 26.665871750781317 22.660123253799906 26-27 21.78969035934382 28.023922784381156 27.287788836870707 22.898598019404314 28-29 22.20827632949682 27.220639363397893 28.21658529238261 22.354499014722677 30-31 21.502893075445886 27.255155447893266 28.490203707655038 22.75174776900581 32-33 21.036612152172 29.351223124521482 26.97839920675762 22.63376551654889 34-35 21.619620197557516 27.93857392090169 27.985641308849925 22.456164572690874 36-37 22.83835176283057 27.544462992481773 26.75122061426079 22.86596463042687 38-39 21.441391688526853 27.604709249055514 27.78482045360411 23.169078608813525 40-41 21.163380317045924 26.406687334479685 28.73495412498588 23.69497822348851 42-43 21.67421836757747 26.37970203205603 28.94142306678549 23.00465653358101 44-45 20.439170107815695 26.72925583321828 29.365657123492273 23.46591693547375 46-47 21.582593852371566 25.673691212832452 28.785786903969978 23.957928030826 48-49 20.884741380392356 25.86509859048862 29.260853739660863 23.98930628945816 50-51 19.854906932084898 26.09415987850338 29.58028441253624 24.47064877687548 52-53 19.472092176772556 26.599977407653785 30.664089465691013 23.263840949882646 54-55 18.651236930955278 26.737414180462643 29.902225346102192 24.709123542479887 56-57 19.937117969701156 26.38472255343717 28.741229776712313 24.93692970014936 58-59 19.06354724938185 27.126504587501408 29.235123567582495 24.57482459553425 60-61 19.816625456553663 27.515594994540184 28.064086955430323 24.60369259347583 62-63 18.75415761926876 28.293775808617728 27.5419527317912 25.410113840322317 64-65 19.299511754295683 29.042461059581036 26.188922219572504 25.469104966550777 66-67 19.36164070638736 28.56676665871751 26.298118559612416 25.77347407528272 68-69 19.145758286998106 28.789552295005837 26.073450227806156 25.9912391901899 70-71 20.00552257351926 28.20215129341182 25.65360912730787 26.13871700576105 72-73 19.992343704893752 27.651776637003756 25.417017057221393 26.9388626008811 74-75 19.959710315916308 27.823729494307987 26.010693710541837 26.20586647923387 76 20.210108819800936 28.252984072395922 25.848154330827256 25.68875277697589 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 2.0 17 3.0 18 2.0 19 3.0 20 8.5 21 12.0 22 15.5 23 30.5 24 50.5 25 59.0 26 123.5 27 194.0 28 312.5 29 425.0 30 496.0 31 663.0 32 992.5 33 1226.0 34 1332.5 35 1685.0 36 2197.5 37 2464.0 38 2823.5 39 3817.5 40 4952.5 41 5688.5 42 5924.0 43 5876.5 44 6060.0 45 6401.0 46 6511.0 47 6433.5 48 5942.5 49 5159.5 50 4790.0 51 4475.0 52 3653.0 53 2775.0 54 2404.0 55 2304.5 56 1782.5 57 1210.5 58 1061.0 59 800.5 60 475.0 61 332.5 62 255.0 63 203.5 64 126.5 65 105.5 66 78.0 67 46.0 68 39.0 69 24.0 70 11.5 71 7.0 72 5.0 73 3.0 74 2.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 79673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.11386542492438 #Duplication Level Percentage of deduplicated Percentage of total 1 57.89802940256491 20.909216422125436 2 14.457998818336634 10.4426844727825 3 7.934521947659263 8.596387734866266 4 4.997740937684634 7.219509746087131 5 3.28085357800716 5.924215229751609 6 2.4258853786535988 5.256485886059267 7 1.685608035310882 4.261167522247185 8 1.376290272130122 3.976252933867182 9 1.0217912626420602 3.3210748936277033 >10 4.876099120703437 27.701981850815205 >50 0.03475480485177076 0.750567946481242 >100 0.006950960970354152 0.6802806471452061 >500 0.003475480485177076 0.960174714144064 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 765 0.960174714144064 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 368 0.46188796706537977 Illumina PCR Primer Index 9 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 174 0.2183926800798263 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 99 0.12425790418334944 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002510260690572716 0.0 2 0.0 0.0 0.0 0.002510260690572716 0.0 3 0.0 0.0 0.0 0.003765391035859074 0.0 4 0.0 0.0 0.0 0.00627565172643179 0.0 5 0.0 0.0 0.0 0.00627565172643179 0.0 6 0.0 0.0 0.0 0.00627565172643179 0.0 7 0.0 0.0 0.0 0.00627565172643179 0.0 8 0.0 0.0 0.0 0.008785912417004505 0.0 9 0.0 0.0 0.0 0.013806433798149938 0.0 10 0.0 0.0 0.0 0.021337215869868087 0.0 11 0.0 0.0 0.0 0.02510260690572716 0.0 12 0.0 0.0 0.0 0.02510260690572716 0.0 13 0.0 0.0 0.0 0.026357737251013518 0.0 14 0.0 0.0 0.0 0.028867997941586232 0.0 15 0.0 0.0 0.0 0.03012312828687259 0.0 16 0.0 0.0 0.0 0.032633388977445305 0.0 17 0.0 0.0 0.0 0.04016417104916346 0.0 18 0.0 0.0 0.0 0.051460344156740674 0.0 19 0.0 0.0 0.0 0.05648086553788611 0.0 20 0.0 0.0 0.0 0.058991126228458826 0.0 21 0.0 0.0 0.0 0.061501386919031543 0.0 22 0.0 0.0 0.0 0.07405269037189512 0.0 23 0.0 0.0 0.0 0.08032834209832691 0.0 24 0.0 0.0 0.0 0.08283860278889962 0.0 25 0.0 0.0 0.0 0.08409373313418599 0.0 26 0.0 0.0 0.0 0.08911425451533142 0.0 27 0.0 0.0 0.0 0.09790016693233593 0.0 28 0.0 0.0 0.0 0.12802329521920852 0.0 29 0.0 0.0 0.0 0.1493605110890766 0.0 30 0.0 0.0 0.0 0.17571824834009012 0.0 31 0.0 0.0 0.0 0.2121170283533945 0.0 32 0.0 0.0 0.0 0.23847476560440803 0.0 33 0.0 0.0 0.0 0.2886799794158623 0.0 34 0.0 0.0 0.0 0.3564570180613257 0.0 35 0.0 0.0 0.0 0.4455712725766571 0.0 36 0.0 0.0 0.0 0.5961869140110201 0.0 37 0.0 0.0 0.0 0.8836117630815961 0.0 38 0.0 0.0 0.0 1.241323911488208 0.0 39 0.0 0.0 0.0 1.6944259661365833 0.0 40 0.0 0.0 0.0 2.328266790506194 0.0 41 0.0 0.0 0.0 3.018588480413691 0.0 42 0.0 0.0 0.0 3.798024424836519 0.0 43 0.0 0.0 0.0 4.534785937519612 0.0 44 0.0 0.0 0.0 5.358151444027462 0.0 45 0.0 0.0 0.0 6.097423217401127 0.0 46 0.0 0.0 0.0 6.9132579418372595 0.0 47 0.0 0.0 0.0 7.6249168476146245 0.0 48 0.0 0.0 0.0 8.374229663750581 0.0 49 0.0 0.0 0.0 9.168727172316846 0.0 50 0.0 0.0 0.0 9.827670603592184 0.0 51 0.0 0.0 0.0 10.630954024575452 0.0 52 0.0 0.0 0.0 11.318765453792377 0.0 53 0.0 0.0 0.0 12.076864182345336 0.0 54 0.0 0.0 0.0 12.829942389517152 0.0 55 0.0 0.0 0.0 13.424874173182886 0.0 56 0.0 0.0 0.0 13.997213610633464 0.0 57 0.0 0.0 0.0 14.632309565348361 0.0 58 0.0 0.0 0.0 15.163229701404491 0.0 59 0.0 0.0 0.0 15.785774352666525 0.0 60 0.0 0.0 0.0 16.34305222597367 0.0 61 0.0 0.0 0.0 16.991954614486716 0.0 62 0.0 0.0 0.0 17.60696848367703 0.0 63 0.0 0.0 0.0 18.166756617674746 0.0 64 0.0 0.0 0.0 18.820679527568938 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTAG 15 0.0022172893 70.0 34 ATCAGTA 15 0.0022172893 70.0 21 GATCAGT 15 0.0022172893 70.0 20 GCGGGTA 15 0.0022172893 70.0 33 TGGATTC 15 0.0022172893 70.0 26 ACATCCA 15 0.0022172893 70.0 36 ATTGGCG 45 2.6609996E-8 54.444447 21 ATAAAGA 45 2.6609996E-8 54.444447 1 ATTTAAT 20 0.006928426 52.499996 34 TCGTTTT 20 0.006928426 52.499996 54 TGCCCGT 20 0.006928426 52.499996 59 CTCAAGC 20 0.006928426 52.499996 12 GATCGGA 20 0.006928426 52.499996 39 GTCGAAA 20 0.006928426 52.499996 43 CGAAATC 20 0.006928426 52.499996 45 GCGAATA 20 0.006928426 52.499996 57 GAGTCGA 20 0.006928426 52.499996 41 GGCACGG 20 0.006928426 52.499996 12 AGGACGC 20 0.006928426 52.499996 12 GGATCGG 20 0.006928426 52.499996 38 >>END_MODULE