FastQCFastQC Report
Wed 25 May 2016
SRR1780683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424048
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT5120.12074104818322455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC404.073081E-738.55187224
CATAGCG250.0023396635.23491735
TGCATAG250.00237517935.126892
CGCGAGC451.0204694E-634.28455714
TACTGAT451.0318636E-634.23592423
CTACCCG451.0318636E-634.23592410
ACCGTTA353.184643E-431.47836713
TACCGTT353.191282E-431.46719412
TCGGTGG353.2380747E-431.38920442
GGACCGG353.2537975E-431.3632933
GCGAGCC502.3191515E-630.86340915
TACCCGC502.3493176E-630.8123311
ACGAGAG300.005703188529.37285624
ACGAGAC300.005703188529.37285624
CGAAACT454.0686587E-529.32774225
GTGCATA300.005772795529.300051
GATGTAG454.094973E-529.3000491
GAGCTCG300.005776126429.2965941
TCACTCG300.005782793729.28967743
GCGTATC300.005782793729.28967744
Produced by FastQC (version 0.11.5)