FastQCFastQC Report
Wed 25 May 2016
SRR1780683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424048
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT56681.336641134965853No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT38520.9083877296909784RNA PCR Primer, Index 14 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC12410.2926555484284798No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC4710.11107233143417726No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG4650.1096573972757801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT302.517596E-644.01604514
CGTGCGA357.2611456E-637.7280410
GTAGCGA250.00234687335.21283711
ATAGCGA250.002352312735.1962283
CCGGTGA505.93609E-835.19622836
CCGTCTT6150.034.69546544
GTACAAG702.7284841E-1134.5881161
GCCGTCT6600.032.3298743
CAAGGAA756.184564E-1132.263214
AGTTAGA353.2163883E-431.42520136
TGCCGTC7250.029.43132842
CGGCCTC300.005730639729.3440311
GATGTAT454.0630897E-529.33364724
CCGTGCG454.0630897E-529.3336479
CCAACGT454.0630897E-529.33364722
TTTTCGA300.005740560629.33364721
GAATGCG454.0663606E-529.33018941
GGTCACA454.0663606E-529.33018942
CCCGTGC454.0663606E-529.3301898
ATGCCTT300.00574387129.3301895
Produced by FastQC (version 0.11.5)