FastQCFastQC Report
Wed 25 May 2016
SRR1780682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56522
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT150.002214830169.9929259
GTGCACG150.002214830169.9929255
CATGCAT207.867593E-569.9929226
CACGCGA150.002214830169.9929258
GCACGCG150.002214830169.9929257
CTCCATG150.002214830169.9929223
CGCGATA150.002214830169.9929260
TATTCAG150.002214830169.9929248
GGTATTT150.002214830169.9929235
CGGGAGC150.002214830169.9929215
TTTGACG150.002214830169.9929210
TGCACGC150.002214830169.9929256
CCTTATC200.006728813552.8689841
GACAGTT200.006920811752.4946926
GGAGCTC200.006920811752.4946917
TTCAGCT200.006920811752.4946950
CACGGGA200.006920811752.4946913
TTTCGTC200.006920811752.4946940
AGCTTCC200.006920811752.4946953
ATAGCGA200.006920811752.4946926
Produced by FastQC (version 0.11.5)