##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780682_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56522 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.820264675701498 34.0 31.0 34.0 12.0 34.0 2 31.200753688829128 34.0 32.0 34.0 23.0 34.0 3 31.488199285234067 34.0 33.0 34.0 23.0 34.0 4 31.734899685078375 34.0 34.0 34.0 23.0 34.0 5 31.796610169491526 34.0 34.0 34.0 23.0 34.0 6 34.57703195216022 38.0 36.0 38.0 25.0 38.0 7 33.961041718268994 38.0 34.0 38.0 23.0 38.0 8 32.881798237854284 37.0 33.0 38.0 12.0 38.0 9 33.59132727079721 37.0 34.0 38.0 21.0 38.0 10-11 33.951762145713175 37.0 34.0 38.0 22.5 38.0 12-13 34.3623810197799 38.0 35.0 38.0 24.5 38.0 14-15 33.91057464350165 37.5 34.0 38.0 22.0 38.0 16-17 33.76146456247125 37.0 34.0 38.0 21.5 38.0 18-19 33.75146845476098 37.0 34.0 38.0 22.0 38.0 20-21 33.81573546583631 37.0 34.0 38.0 21.5 38.0 22-23 33.7910282721772 37.0 34.0 38.0 22.0 38.0 24-25 34.228079331941544 38.0 34.5 38.0 23.5 38.0 26-27 34.26958529422172 38.0 34.5 38.0 23.5 38.0 28-29 34.38635398605852 38.0 35.0 38.0 24.0 38.0 30-31 34.253405753511906 38.0 35.0 38.0 23.5 38.0 32-33 34.005528820636215 38.0 34.0 38.0 22.0 38.0 34-35 33.86178833020771 37.0 34.0 38.0 22.5 38.0 36-37 33.843167262304945 37.0 34.0 38.0 22.0 38.0 38-39 34.38054208980574 38.0 35.0 38.0 24.0 38.0 40-41 33.88111708715191 37.5 34.0 38.0 22.0 38.0 42-43 34.202558295884785 38.0 34.5 38.0 23.5 38.0 44-45 33.24694809100881 37.0 34.0 38.0 21.0 38.0 46-47 33.968463607091046 37.0 34.0 38.0 23.0 38.0 48-49 33.72401011995329 37.0 34.0 38.0 22.5 38.0 50-51 33.65822157743887 37.0 34.0 38.0 22.0 38.0 52-53 33.703133293230955 37.0 34.0 38.0 22.5 38.0 54-55 33.45972364742932 37.0 34.0 38.0 21.0 38.0 56-57 33.53083754998053 37.0 34.0 38.0 22.0 38.0 58-59 33.39126357878348 37.0 33.0 38.0 22.0 38.0 60-61 33.41111425639574 37.0 33.5 38.0 21.5 38.0 62-63 33.493949258695736 37.0 33.5 38.0 21.5 38.0 64-65 33.50349421464209 37.0 33.5 38.0 21.5 38.0 66-67 33.44656063125862 37.0 33.5 38.0 21.5 38.0 68-69 32.9924542655957 37.0 32.0 38.0 16.5 38.0 70-71 32.884956300201694 37.0 32.0 38.0 19.5 38.0 72-73 32.97433742613495 37.0 32.0 38.0 19.5 38.0 74-75 32.6038445207176 37.0 31.0 38.0 19.5 38.0 76 31.745391175117653 36.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.855901602222776 1101 2 -1.1975216939996365 1101 3 -0.32927628124998876 1101 4 -2.954479126322507 1101 5 -1.2886453203068662 1101 6 -2.6910447030685987 1101 7 -1.6436678468185448 1101 8 -1.3143313898997064 1101 9 -1.0504500708669013 1101 10-11 -1.3343376407600758 1101 12-13 -1.0562836038418624 1101 14-15 -0.5346121446175189 1101 16-17 -2.450532447370911 1101 18-19 -0.5503616281351 1101 20-21 -0.7242371413243731 1101 22-23 -0.34969743098390893 1101 24-25 -1.847934011262609 1101 26-27 -1.5342351555425253 1101 28-29 -0.6961000054685584 1101 30-31 -1.9189544339390707 1101 32-33 -0.07347235629754323 1101 34-35 -1.0728456251455896 1101 36-37 -0.14986142979793726 1101 38-39 -1.375370547731876 1101 40-41 0.03866038393954341 1101 42-43 -0.800034981757328 1101 44-45 -0.21075960935491622 1101 46-47 0.19206059128215713 1101 48-49 -1.2808018128475638 1101 50-51 -1.0198940933186975 1101 52-53 -0.013218672004477128 1101 54-55 -1.6983836076406646 1101 56-57 -0.312048289336083 1101 58-59 -1.1721018705877313 1101 60-61 -0.5736797100110067 1101 62-63 -2.249299078404839 1101 64-65 -0.35841495899867226 1101 66-67 -1.8042567186721712 1101 68-69 0.021441370221467082 1101 70-71 -0.24236122470008326 1101 72-73 -0.17185161665221216 1101 74-75 -2.1212012384789674 1101 76 -3.34477402233793 1102 1 0.45913218156100655 1102 2 -0.621085883188826 1102 3 -0.026066821790529104 1102 4 -0.31828824794412824 1102 5 -0.19658450949605566 1102 6 0.9204417834178855 1102 7 1.33796222074902 1102 8 2.057712529019213 1102 9 1.1353607399439127 1102 10-11 0.12353820383452074 1102 12-13 -3.3992945835715886 1102 14-15 -0.8922979554283259 1102 16-17 -2.505114373046588 1102 18-19 -0.014783080837801776 1102 20-21 0.5883684329999497 1102 22-23 -0.48145418774066684 1102 24-25 0.33280923198063306 1102 26-27 -5.012697993380364 1102 28-29 0.08177161615306261 1102 30-31 0.34286989038525206 1102 32-33 -6.962408167108357 1102 34-35 -0.09448836163207375 1102 36-37 -0.027395213581719702 1102 38-39 0.9476868847005591 1102 40-41 0.9139771069125189 1102 42-43 0.3255984641886158 1102 44-45 -5.822773967463025 1102 46-47 -0.07240309115027799 1102 48-49 -0.3101514750097252 1102 50-51 -6.776017404129508 1102 52-53 -2.0011831990315017 1102 54-55 0.3920935883052863 1102 56-57 -1.780798289336083 1102 58-59 0.9690510010338897 1102 60-61 0.6706192764754775 1102 62-63 -5.178986578404839 1102 64-65 -1.1065146211608337 1102 66-67 -0.300983914618115 1102 68-69 -6.976869440589347 1102 70-71 -0.20709940037576047 1102 72-73 -0.013067832868429718 1102 74-75 -6.582561035776266 1102 76 -2.7573584817973895 1103 1 0.5814090038378303 1103 2 -7.851218145304983E-5 1103 3 0.17507977935607144 1103 4 1.1154545857987053 1103 5 1.0304834675719228 1103 6 -1.002408339432236 1103 7 0.9294855622723617 1103 8 1.6660663373730173 1103 9 0.1003075048906723 1103 10-11 1.4047011849974993 1103 12-13 -0.45895879323580147 1103 14-15 -0.14436593249630647 1103 16-17 -0.46580233373455115 1103 18-19 -0.4840500751047969 1103 20-21 0.08449865413017221 1103 22-23 -0.12166712795360013 1103 24-25 0.9819902311616318 1103 26-27 -0.5998601555425225 1103 28-29 1.0154530248344713 1103 30-31 0.8170304145457763 1103 32-33 -3.7872034169036013 1103 34-35 -0.07012308726680061 1103 36-37 0.21623705505054858 1103 38-39 0.7766180886317642 1103 40-41 1.215246558181967 1103 42-43 0.9879858515760063 1103 44-45 -4.426787071476127 1103 46-47 0.16684752310033701 1103 48-49 0.9247379598797139 1103 50-51 0.23403583092373026 1103 52-53 0.44251049466218717 1103 54-55 1.2256930969047914 1103 56-57 1.0972320136942173 1103 58-59 1.0466718036546965 1103 60-61 1.4629206687768743 1103 62-63 0.8588922094739502 1103 64-65 1.1404960258498136 1103 66-67 0.5419317282975271 1103 68-69 -3.3463616600815627 1103 70-71 1.0852429419665768 1103 72-73 0.9387544439538473 1103 74-75 -1.974657677872905 1103 76 0.09907067463176844 1104 1 -2.531657971649526 1104 2 0.5888039429430378 1104 3 0.2405883684315384 1104 4 0.061295157116983745 1104 5 0.006935889884218227 1104 6 8.342778231238412E-4 1104 7 1.4789933792961065 1104 8 1.5400674954506144 1104 9 0.3436582093878755 1104 10-11 -0.9073621232441482 1104 12-13 -0.3781635799565066 1104 14-15 0.6486083967837573 1104 16-17 -0.29122213686135723 1104 18-19 1.2555947811005694 1104 20-21 1.3492351914781793 1104 22-23 0.4886783651944313 1104 24-25 0.18988446007496407 1104 26-27 0.7033245578332696 1104 28-29 0.1806913958053329 1104 30-31 -1.5308174912639103 1104 32-33 -0.8291451269981849 1104 34-35 0.7056923923703309 1104 36-37 0.48807646829123996 1104 38-39 0.7803818408031589 1104 40-41 1.372532796359927 1104 42-43 1.0412767220643318 1104 44-45 -3.1987422924759343 1104 46-47 0.9372586406452115 1104 48-49 1.2978705597639006 1104 50-51 1.718710604133534 1104 52-53 0.056098605065585616 1104 54-55 0.6889820372637985 1104 56-57 1.0305074431479895 1104 58-59 0.7128752392848767 1104 60-61 0.7032410702437701 1104 62-63 0.8735611922957958 1104 64-65 0.3964118722115231 1104 66-67 1.2755352797354718 1104 68-69 -2.578479011944136 1104 70-71 -0.7909778648274717 1104 72-73 1.0303776827108422 1104 74-75 -0.26774781491209154 1104 76 -0.2437588949493943 1105 1 -0.5882879658591378 1105 2 0.34537603327309085 1105 3 -0.1582535539772607 1105 4 0.24878791913203813 1105 5 0.3335137706022273 1105 6 -0.032711369735267226 1105 7 1.0688795016663022 1105 8 0.4781875494942298 1105 9 1.009398413981586 1105 10-11 1.0501557304520475 1105 12-13 -0.8559284902054998 1105 14-15 -0.06254775067812801 1105 16-17 0.8978340299018157 1105 18-19 0.12201053095581216 1105 20-21 -0.39731952768801193 1105 22-23 0.448029841743363 1105 24-25 0.2728993220707281 1105 26-27 0.7243822686999053 1105 28-29 -0.4209106115291661 1105 30-31 0.6624849600003202 1105 32-33 1.2400693103691225 1105 34-35 0.7753314581877504 1105 36-37 1.4101764489899438 1105 38-39 1.1947999068135786 1105 40-41 1.2788829218183295 1105 42-43 1.0273797909699454 1105 44-45 2.2550311103420526 1105 46-47 1.9426050988579107 1105 48-49 0.46413189927364584 1105 50-51 0.05221764910554327 1105 52-53 -0.6999137477620536 1105 54-55 -0.9197614485497567 1105 56-57 0.28511080157300484 1105 58-59 -0.029085772102881435 1105 60-61 0.5659509718071689 1105 62-63 1.2588922094739488 1105 64-65 0.5738293591831436 1105 66-67 1.529810516176319 1105 68-69 1.8687898550699487 1105 70-71 2.097364154087792 1105 72-73 0.9690574742568785 1105 74-75 -1.2261728293880552 1105 76 -1.125171749610658 1106 1 0.041120209857762546 1106 2 0.3013247825104308 1106 3 -0.9217678919882424 1106 4 0.1959508233419207 1106 5 0.25602749848507855 1106 6 0.21247878686428834 1106 7 1.7452268679465561 1106 8 1.2663103886237863 1106 9 1.5997177143679977 1106 10-11 0.38800401192448675 1106 12-13 0.4092942065608227 1106 14-15 0.7314323184697287 1106 16-17 0.6705613026290891 1106 18-19 0.9303692862944288 1106 20-21 -0.022029716827724144 1106 22-23 0.6645643139825381 1106 24-25 1.3999686733011458 1106 26-27 0.30752239479304677 1106 28-29 -0.43833993835446705 1106 30-31 -0.2079645010531621 1106 32-33 1.3425301604809832 1106 34-35 1.494347118142997 1106 36-37 1.3226637379872983 1106 38-39 0.6603886804560375 1106 40-41 0.3554336641408895 1106 42-43 1.1539813773030758 1106 44-45 2.0303005835980983 1106 46-47 1.1360360526915514 1106 48-49 1.254695250910821 1106 50-51 1.0305987758088193 1106 52-53 0.5946357743042441 1106 54-55 1.0073892530975854 1106 56-57 1.5270876167041862 1106 58-59 -0.056094110520611196 1106 60-61 1.3250723873044308 1106 62-63 1.4837650994475098 1106 64-65 1.7737683463704528 1106 66-67 1.4905996152204466 1106 68-69 1.983270229281871 1106 70-71 0.9098514430851523 1106 72-73 0.9002960343545041 1106 74-75 -0.5788878995527966 1106 76 0.7927050045077095 1107 1 -0.37616675373792674 1107 2 -0.23644214854509116 1107 3 0.0841706884469815 1107 4 -0.3027272323831127 1107 5 0.7819986190870729 1107 6 0.09456135753746508 1107 7 -0.009908377121575995 1107 8 0.029702701009384214 1107 9 -1.287571282988111 1107 10-11 -0.7104503301540106 1107 12-13 -0.5498678841448879 1107 14-15 -1.1716386597690374 1107 16-17 0.4220764541442392 1107 18-19 0.8523135612588391 1107 20-21 0.3057107753422983 1107 22-23 -1.3246974309839032 1107 24-25 -0.5786158294444306 1107 26-27 0.1577156020332282 1107 28-29 -0.7875772781958332 1107 30-31 -0.7496362521208866 1107 32-33 0.46128143158124146 1107 34-35 0.49654357939986227 1107 36-37 -0.5837629449494557 1107 38-39 0.15237566438933925 1107 40-41 0.3213071642425689 1107 42-43 -0.05746869387854048 1107 44-45 -0.5055749502640055 1107 46-47 -0.4573949011420808 1107 48-49 -0.8692014340596828 1107 50-51 0.6097934066813053 1107 52-53 0.41826807041976366 1107 54-55 0.004480975692672473 1107 56-57 0.7851108015730048 1107 58-59 0.8133384703213622 1107 60-61 -0.8279884221322149 1107 62-63 -1.0865623359805952 1107 64-65 0.0859505713043589 1107 66-67 0.5995074858732821 1107 68-69 0.9081837944638949 1107 70-71 -0.4420297853061399 1107 72-73 -0.924881919682516 1107 74-75 -0.541324344539575 1107 76 -1.3736565980955042 1108 1 -0.5121516022227759 1108 2 -0.3506466939996358 1108 3 -1.060526281249988 1108 4 -0.7091666263225065 1108 5 0.19885467969313453 1108 6 0.4464552969314042 1108 7 1.4203946531814537 1108 8 1.207543610100295 1108 9 -0.7442000708669028 1108 10-11 0.4445686092399228 1108 12-13 0.5741851461581362 1108 14-15 1.009137855382484 1108 16-17 -1.0575636973709095 1108 18-19 -0.07458037813510288 1108 20-21 0.7390441086756212 1108 22-23 -0.6059474309839032 1108 24-25 -0.6385590112626076 1108 26-27 -0.2779851555425239 1108 28-29 0.42264999453144014 1108 30-31 0.5029205660609293 1108 32-33 0.32652764370246246 1108 34-35 -0.23612687514558672 1108 36-37 -0.002986429797935841 1108 38-39 1.0090044522681225 1108 40-41 0.053504133939540566 1108 42-43 0.5077775182426691 1108 44-45 -0.16466585935491906 1108 46-47 0.5162793412821571 1108 48-49 0.02076068715243906 1108 50-51 0.20666840668130249 1108 52-53 0.11334382799552145 1108 54-55 0.16489764235933535 1108 56-57 0.49107671066391845 1108 58-59 -0.536945620587737 1108 60-61 0.5622577899889905 1108 62-63 0.6022634215951612 1108 64-65 0.4400225410013263 1108 66-67 0.3418370313278345 1108 68-69 1.1401913702214657 1108 70-71 1.5748262752999196 1108 72-73 0.5562733833477864 1108 74-75 0.986611261521027 1108 76 1.5396009776620687 1109 1 -1.4849080124791847 1109 2 -0.7011274632304065 1109 3 -0.2675775633012698 1109 4 -0.2864102160660984 1109 5 -0.404831217742764 1109 6 0.19677580975191233 1109 7 -2.319348936562136 1109 8 0.27981925112593586 1109 9 -0.16262955804639034 1109 10-11 0.5353538656501797 1109 12-13 -0.6214879307649426 1109 14-15 -0.6455897087200881 1109 16-17 -1.467259210191422 1109 18-19 -0.8601573012120198 1109 20-21 0.4659671855987071 1109 22-23 0.6503025690160911 1109 24-25 -0.02581862664722223 1109 26-27 0.0014218957395257803 1109 28-29 -0.684080774699332 1109 30-31 -0.048762126246764126 1109 32-33 0.36122315652296777 1109 34-35 -0.8547967469404654 1109 36-37 -0.054508865695375164 1109 38-39 -0.12897631696264966 1109 40-41 -0.5461753532399456 1109 42-43 -0.39598850739835 1109 44-45 -0.4518453465344052 1109 46-47 -1.1239450176922006 1109 48-49 0.10842094356269882 1109 50-51 0.9386395605274558 1109 52-53 0.8367412638929608 1109 54-55 0.12563482184651775 1109 56-57 0.3666376080998148 1109 58-59 -0.5147501077672203 1109 60-61 0.4501584310146356 1109 62-63 -0.8841147835330432 1109 64-65 -0.8129422025884168 1109 66-67 0.1385517749175733 1109 68-69 0.3467618830419781 1109 70-71 -0.7493724426487987 1109 72-73 -0.6141593089599056 1109 74-75 1.3878131845979524 1109 76 -0.07995030438921091 1110 1 0.3828955675885446 1110 2 -0.2133432348801385 1110 3 -0.25911118691036705 1110 4 -0.24383643764326024 1110 5 0.02247889352961252 1110 6 0.13206850447856766 1110 7 0.6053003135588142 1110 8 1.6104917233078382 1110 9 -1.5811104482253953 1110 10-11 -1.0591067052254814 1110 12-13 -0.3095019607601017 1110 14-15 -0.4722104150577664 1110 16-17 -0.6218915275595904 1110 18-19 -0.02984767373258279 1110 20-21 -1.5339118661671378 1110 22-23 -0.394508751738627 1110 24-25 -0.11640885402989909 1110 26-27 -1.4130677027123397 1110 28-29 -0.44000723817296006 1110 30-31 -0.1339701572095109 1110 32-33 0.5425850336395683 1110 34-35 0.2583503261122715 1110 36-37 -1.0667246373451036 1110 38-39 -0.1836449188010576 1110 40-41 -0.5448449226642325 1110 42-43 -0.009498425153559253 1110 44-45 0.6972838261796781 1110 46-47 -0.48352411783734084 1110 48-49 0.5815704355801117 1110 50-51 -0.05876005243819549 1110 52-53 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2.1889055692165087 2116 46-47 0.474315055567871 2116 48-49 0.9020106871524334 2116 50-51 0.6847934066813082 2116 52-53 1.3401295422812396 2116 54-55 -0.33242378621208957 2116 56-57 0.5513445678067725 2116 58-59 -0.466856334873448 2116 60-61 -1.00158149572529 2116 62-63 1.1960134215951612 2116 64-65 1.730647541001325 2116 66-67 0.848533459899258 2116 68-69 0.20894137022146708 2116 70-71 -2.7957094389857957 2116 72-73 0.4852912404906462 2116 74-75 -1.3566923099075368 2116 76 -0.2264704509093569 2117 1 0.11151861054318246 2117 2 0.6478905400429156 2117 3 0.9825588251329904 2117 4 0.7288652885711109 2117 5 -0.7857641146331069 2117 6 -0.5660447030685987 2117 7 -2.8023003822795367 2117 8 -2.1236177374174368 2117 9 0.8210038298423186 2117 10-11 0.3339081482470192 2117 12-13 0.9598677702716145 2117 14-15 0.22791001850304582 2117 16-17 1.0287173309978854 2117 18-19 1.007955082857812 2117 20-21 -0.1978353239484889 2117 22-23 0.5554444129877254 2117 24-25 0.4211262724253402 2117 26-27 0.8561682132517987 2117 28-29 0.6454336824747102 2117 30-31 1.1976235802453274 2117 32-33 1.445033849376216 2117 34-35 0.47887755747852623 2117 36-37 -0.9182568198688585 2117 38-39 0.679261544466712 2117 40-41 -0.6494213979753525 2117 42-43 -0.4120141484239994 2117 44-45 1.139944069723093 2117 46-47 0.2048874973105228 2117 48-49 -0.09125172419508232 2117 50-51 1.1863891513621567 2117 52-53 1.0666239698394904 2117 54-55 -1.3508381732435026 2117 56-57 -1.1079259489105482 2117 58-59 -1.000974875906877 2117 60-61 1.4926434282868684 2117 62-63 0.255410584715726 2117 64-65 0.7364986048311195 2117 66-67 -0.49688194030337485 2117 68-69 -0.23875366523952835 2117 70-71 1.2824060625339584 2117 72-73 1.2838221422130331 2117 74-75 0.5711192402444354 2117 76 -0.017757178366299087 2118 1 1.0704532928821209 2118 2 0.6782431661402235 2118 3 0.6543338586101513 2118 4 0.541095611439733 2118 5 -0.07907364198518962 2118 6 1.2241650871411949 2118 7 1.384031016817822 2118 8 -2.8542133829067 2118 9 0.06580867039183858 2118 10-11 0.5918806721769911 2118 12-13 1.454561020284018 2118 14-15 -0.3445990327294126 2118 16-17 1.0901417222095091 2118 18-19 -0.5926747837295068 2118 20-21 -1.6336831640516465 2118 22-23 -0.08483729112376892 2118 24-25 0.9864191355905376 2118 26-27 1.214125858443488 2118 28-29 0.20403111341256164 2118 30-31 0.03777633529170288 2118 32-33 0.5922837625835768 2118 34-35 1.1035365863928703 2118 36-37 0.22229766111115623 2118 38-39 0.021373333387003868 2118 40-41 -0.4628420199066099 2118 42-43 0.35954762313777877 2118 44-45 0.981138336449284 2118 46-47 0.5234908797436972 2118 48-49 -0.10603127088952391 2118 50-51 0.014688511576409269 2118 52-53 -0.04233799018629725 2118 54-55 0.8263923976040957 2118 56-57 1.9053905218527305 2118 58-59 1.8063454633283555 2118 60-61 -1.4172658114096066 2118 62-63 1.0815029320846676 2118 64-65 0.7199832053369946 2118 66-67 -1.6872058008400046 2118 68-69 1.5210043072844002 2118 70-71 -0.04715799043434998 2118 72-73 -1.7889844837850788 2118 74-75 1.1465544433392196 2118 76 -0.5811158055547132 2119 1 -0.0637820370053852 2119 2 0.1224511320873205 2119 3 0.46374908106885115 2119 4 -0.18217387269931962 2119 5 0.5976771434612509 2119 6 0.839752398380675 2119 7 -1.7694604192823107 2119 8 0.551837088361161 2119 9 -0.8638558679683541 2119 10-11 -0.2717810284412323 2119 12-13 0.15289891427407554 2119 14-15 -0.5724291736030267 2119 16-17 -0.47798942200859074 2119 18-19 -1.1870803781351 2119 20-21 -1.6073327029185762 2119 22-23 0.8006648878566693 2119 24-25 -0.32710067792928044 2119 26-27 -0.491889141049775 2119 28-29 0.33152680612564467 2119 30-31 0.30115426171310133 2119 32-33 -0.11717706644246562 2119 34-35 0.087650298767457 2119 36-37 -0.3287563573341714 2119 38-39 -0.13510786657245433 2119 40-41 -0.7039234022923466 2119 42-43 -1.0605648730616721 2119 44-45 -1.8161151347172364 2119 46-47 -0.402651818138132 2119 48-49 -0.40523568965915757 2119 50-51 0.9931267400146311 2119 52-53 -0.5683590705551964 2119 54-55 -0.4614610532928367 2119 56-57 0.594201710663917 2119 58-59 1.6209801040499556 2119 60-61 1.0645675725976886 2119 62-63 -0.0775373030425186 2119 64-65 0.3237634830303122 2119 66-67 0.5543823211829064 2119 68-69 -0.6153339920973764 2119 70-71 -1.4227280725261693 2119 72-73 -1.4617066891159816 2119 74-75 -0.44830721673983476 2119 76 -0.029783080308945387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 11.0 13 71.0 14 223.0 15 377.0 16 480.0 17 520.0 18 533.0 19 493.0 20 468.0 21 417.0 22 448.0 23 464.0 24 527.0 25 566.0 26 745.0 27 926.0 28 1052.0 29 1276.0 30 1592.0 31 2007.0 32 2666.0 33 3493.0 34 4765.0 35 6930.0 36 11538.0 37 13933.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.162258501461466 20.9970057745776 10.217794253938832 31.6229414700221 2 19.365910618874064 20.071830437705675 34.230918934220306 26.331340009199955 3 17.51176533031386 25.131807083967306 28.526945260252646 28.829482325466188 4 15.06493046955168 17.58430345706097 35.911680407628886 31.439085665758466 5 13.711475177806873 34.89791585577297 34.80237783517922 16.58823113124093 6 30.036092141113198 35.62683556845122 17.472842433034923 16.86422985740066 7 27.04787516365309 30.913626552492836 21.786207140582427 20.25229114327165 8 24.139273203354445 32.88807897809703 20.54775131807084 22.42489650047769 9 23.491737730441244 16.062772017975302 22.09228265100315 38.353207600580305 10-11 24.715155160822334 25.25122960970949 26.795760942641806 23.23785428682637 12-13 23.565160468490145 23.157354658363115 26.382647464704007 26.89483740844273 14-15 21.185556066664308 24.877039029050636 24.55680973780121 29.380595166483847 16-17 22.48681929160327 27.35395067407381 25.87045752096529 24.28877251335763 18-19 22.061321255440358 26.371147517780685 26.33664767701072 25.23088354976823 20-21 21.637592441881036 26.979760093414956 26.151763914935778 25.23088354976823 22-23 22.96097094936485 26.372916740384277 26.39237818902374 24.273734121227132 24-25 22.447896394324335 27.473373199816002 25.701496762322634 24.37723364353703 26-27 21.19263295707866 27.58748805774743 26.54453133293231 24.675347652241605 28-29 22.22585895757404 26.481723930504934 27.640564735855065 23.651852376065957 30-31 21.928629560171263 26.665723081278088 27.4441810268568 23.961466331693853 32-33 20.726442801033226 28.00502459219419 26.73649198542161 24.53204062135098 34-35 21.93924489579279 26.812568557375887 27.218605144899332 24.029581401931992 36-37 22.286012526096034 26.50030076784261 26.871837514596088 24.341849191465272 38-39 21.957821733130462 26.862106790276354 27.804217826686955 23.37585364990623 40-41 21.942783340999963 25.67495842326882 27.633487845440712 24.748770390290506 42-43 21.3341707653657 25.652843140723963 28.038639821662358 24.974346272247974 44-45 20.477867025229116 25.07519196065249 28.753405753511906 25.693535260606488 46-47 20.60967410919642 25.182229928169562 29.543363646013944 24.66473231662008 48-49 20.07625349421464 25.59888185131453 29.657478503945367 24.66738615052546 50-51 19.503909981953928 25.94034181380701 29.221365132160926 25.334383072078133 52-53 18.85902834294611 26.053572060436643 29.490286967906304 25.597112628710946 54-55 18.55295283252539 26.543646721630516 29.01701992144652 25.88638052439758 56-57 18.650260075722727 26.42333958458653 28.88963589398818 26.03676444570256 58-59 19.15802696295248 25.973957043275185 28.55702204451364 26.310993949258695 60-61 18.771451824068507 26.071264286472523 28.337638441668734 26.81964544779024 62-63 19.45967941686423 25.46530554474364 28.089062665864617 26.98595237252751 64-65 20.150560843565337 25.70945826403878 27.3707582888079 26.769222603587984 66-67 20.0824457733272 25.582958847882242 26.580800396305865 27.7537949824847 68-69 19.80556243586568 25.64665086161141 26.45607020275291 28.09171649977 70-71 20.65390467428612 26.206609815647003 25.416651923144972 27.722833586921908 72-73 20.83171154594671 26.319840062276633 24.887654364672162 27.96079402710449 74-75 20.78040409044266 27.360142953186372 24.90003892289728 26.959414033473696 76 21.193517568380454 27.125720958210962 24.59396341247656 27.086798060932026 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.0 14 2.5 15 2.5 16 3.5 17 5.0 18 5.0 19 8.5 20 10.0 21 8.0 22 11.5 23 28.5 24 53.0 25 64.0 26 91.5 27 161.5 28 241.5 29 279.0 30 324.5 31 430.5 32 650.5 33 810.0 34 874.5 35 1107.0 36 1427.0 37 1579.0 38 1764.0 39 2179.0 40 2603.5 41 3009.0 42 3220.0 43 3438.5 44 3750.0 45 3958.5 46 4074.0 47 4086.0 48 4005.0 49 3676.5 50 3441.0 51 3264.5 52 2893.0 53 2440.0 54 2182.0 55 2078.0 56 1782.5 57 1481.5 58 1372.0 59 1206.0 60 912.0 61 724.0 62 664.0 63 561.0 64 389.5 65 283.0 66 206.0 67 167.0 68 139.0 69 85.5 70 47.5 71 35.0 72 30.5 73 18.5 74 12.0 75 13.0 76 12.0 77 9.0 78 5.0 79 3.0 80 3.0 81 3.5 82 5.0 83 6.0 84 3.5 85 1.0 86 0.5 87 0.0 88 1.0 89 1.5 90 1.5 91 1.5 92 1.0 93 1.0 94 0.5 95 1.0 96 2.0 97 1.5 98 1.5 99 4.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7324581578854251 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 56522.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85577297335551 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28616715471617 50.23884505148438 2 12.42511350509094 16.365309083188848 3 5.225263949708514 10.323413891935882 4 2.5548719877495096 6.73012278404869 5 1.2734062273325633 4.1930575705035205 6 0.6931198452569648 2.7387565903541984 7 0.4808854740348709 2.2168359222957434 8 0.3358138785159713 1.7692226035879837 9 0.22298041533460494 1.3216092848802234 >10 0.502377562259893 4.102827217720534 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012384558225115885 0.0 2 0.0 0.0 0.0 0.012384558225115885 0.0 3 0.0 0.0 0.0 0.012384558225115885 0.0 4 0.0 0.0 0.0 0.012384558225115885 0.0 5 0.0 0.0 0.0 0.012384558225115885 0.0 6 0.0 0.0 0.0 0.017692226035879836 0.0 7 0.0 0.0 0.0 0.019461448639467818 0.0 8 0.0 0.0 0.0 0.02476911645023177 0.0 9 0.0 0.0 0.0 0.033615229468171685 0.0 10 0.0 0.0 0.0 0.0424613424861116 0.0 11 0.0 0.0 0.0 0.058384345918403455 0.0 12 0.0 0.0 0.0 0.06723045893634337 0.0 13 0.0 0.0 0.0 0.08138423976504724 0.0 14 0.0 0.0 0.0 0.08138423976504724 0.0 15 0.0 0.0 0.0 0.08669190757581119 0.0 16 0.0 0.0 0.0 0.09199957538657513 0.0 17 0.0 0.0 0.0 0.10261491100810304 0.0 18 0.0 0.0 0.0 0.11323024662963094 0.0 19 0.0 0.0 0.0 0.12561480485474683 0.0 20 0.0 0.0 0.0 0.13092247266551077 0.0 21 0.0 0.0 0.0 0.1486146987013906 0.0 22 0.0 0.0 0.0 0.16453770213368246 0.0 23 0.0 0.0 0.0 0.17338381515162238 0.0 24 0.0 0.0 0.0 0.18576837337673827 0.0 25 0.0 0.0 0.0 0.19461448639467818 0.0 26 0.0 0.0 0.0 0.19992215420544213 0.0 27 0.0 0.0 0.0 0.22822971586284987 0.0 28 0.0 0.0 0.0 0.2883832843848413 0.0 29 0.0 0.0 0.0 0.32376773645660095 0.0 30 0.0 0.0 0.0 0.3733059693570645 0.0 31 0.0 0.0 0.0 0.4387672056898199 0.0 32 0.0 0.0 0.0 0.49538232900463536 0.0 33 0.0 0.0 0.0 0.5873819043912105 0.0 34 0.0 0.0 0.0 0.6917660380029015 0.0 35 0.0 0.0 0.0 0.8456884045150561 0.0 36 0.0 0.0 0.0 1.1358409115034853 0.0 37 0.0 0.0 0.0 1.604684901454301 0.0 38 0.0 0.0 0.0 2.0965287852517602 0.0 39 0.0 0.0 0.0 2.793602491065426 0.0 40 0.0 0.0 0.0 3.727752025759881 0.0 41 0.0 0.0 0.0 4.607055659743109 0.0 42 0.0 0.0 0.0 5.734050458228654 0.0 43 0.0 0.0 0.0 6.8203531368316765 0.0 44 0.0 0.0 0.0 7.924348041470578 0.0 45 0.0 0.0 0.0 9.084958069424294 0.0 46 0.0 0.0 0.0 10.236721984360072 0.0 47 0.0 0.0 0.0 11.264640317044691 0.0 48 0.0 0.0 0.0 12.232405081207318 0.0 49 0.0 0.0 0.0 13.113477937794134 0.0 50 0.0 0.0 0.0 14.056473585506529 0.0 51 0.0 0.0 0.0 15.100314921623438 0.0 52 0.0 0.0 0.0 16.03092601111072 0.0 53 0.0 0.0 0.0 16.991613884858992 0.0 54 0.0 0.0 0.0 17.980609320264676 0.0 55 0.0 0.0 0.0 18.921835745373482 0.0 56 0.0 0.0 0.0 19.947984855454514 0.0 57 0.0 0.0 0.0 20.765365698312163 0.0 58 0.0 0.0 0.0 21.44297795548636 0.0 59 0.0 0.0 0.0 22.212589788047133 0.0 60 0.0 0.0 0.0 22.982201620607906 0.0 61 0.0 0.0 0.0 23.72350589151127 0.0 62 0.0 0.0 0.0 24.53734828916174 0.0 63 0.0 0.0 0.0 25.321113902551218 0.0 64 0.0 0.0 0.0 26.142033190616043 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGAT 15 0.0022148301 69.99292 59 GTGCACG 15 0.0022148301 69.99292 55 CATGCAT 20 7.867593E-5 69.99292 26 CACGCGA 15 0.0022148301 69.99292 58 GCACGCG 15 0.0022148301 69.99292 57 CTCCATG 15 0.0022148301 69.99292 23 CGCGATA 15 0.0022148301 69.99292 60 TATTCAG 15 0.0022148301 69.99292 48 GGTATTT 15 0.0022148301 69.99292 35 CGGGAGC 15 0.0022148301 69.99292 15 TTTGACG 15 0.0022148301 69.99292 10 TGCACGC 15 0.0022148301 69.99292 56 CCTTATC 20 0.0067288135 52.868984 1 GACAGTT 20 0.0069208117 52.49469 26 GGAGCTC 20 0.0069208117 52.49469 17 TTCAGCT 20 0.0069208117 52.49469 50 CACGGGA 20 0.0069208117 52.49469 13 TTTCGTC 20 0.0069208117 52.49469 40 AGCTTCC 20 0.0069208117 52.49469 53 ATAGCGA 20 0.0069208117 52.49469 26 >>END_MODULE