FastQCFastQC Report
Wed 25 May 2016
SRR1780682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56522
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT7681.3587629595555712No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3730.6599200311383179RNA PCR Primer, Index 42 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC950.16807614734085843No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG860.15215314390856657No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC800.14153780828703866No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT570.10084568840451505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATAG207.863653E-570.0000135
ATAGATG207.863653E-570.0000118
GACCCTC207.863653E-570.000017
GGTCTAC207.863653E-570.0000137
CAGCGCA150.002213941470.026
ATGGCAC150.002213941470.016
GTCTACA150.002213941470.038
AGCGCAA150.002213941470.027
CGTGTTA150.002213941470.026
GTTCAAA150.002213941470.010
CGCAAGT150.002213941470.029
TCTCGTG252.3715638E-455.99999623
GTGTTAC200.00691804452.50000427
CGAGTGA200.00691804452.50000464
GGCGTCA200.00691804452.50000438
ATAGACG200.00691804452.5000043
TGTGCTT200.00691804452.50000416
TCCTATG200.00691804452.50000435
GCGCAAG200.00691804452.50000428
TCGTGTT200.00691804452.50000425
Produced by FastQC (version 0.11.5)