FastQCFastQC Report
Wed 25 May 2016
SRR1780681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207267
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGG301.2941843E-436.6821810
AAATTCG250.00234935.19787630
CTATAAC250.002365759635.1469234
CGATATG250.002365759635.1469238
TGGTTCG353.1736275E-431.48753417
CGACGTT353.200771E-431.44186824
AACCGAC353.2053134E-431.43426934
GTTACTC353.2098612E-431.42667428
ACGTTAC353.2144142E-431.41908526
GAAAGCA300.005702053729.36703712
CGTCTAG300.005742630529.32447623
CGATGCA300.00576300429.30324244
TCGGTGG300.00576300429.30324242
TGTATAC300.00577661829.2891042
ATGACAC300.00577661829.2891045
TTTTAGA300.00577661829.2891042
CCGGAAT406.9358747E-427.5515913
CAAATTC407.0147996E-427.49833929
CTAATTC407.0147996E-427.49833930
TTCTCGG407.0147996E-427.4983391
Produced by FastQC (version 0.11.5)