FastQCFastQC Report
Wed 25 May 2016
SRR1780681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207267
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT20911.0088436654170707No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT8630.41637115411522335TruSeq Adapter, Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC3620.17465394877139148No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC3280.15824998673208954No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG3080.148600597297206No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG3050.14715318888197348No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT2190.10566081431197441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC501.3387762E-939.5945740
CCGTCTT1350.035.8469444
TGCGCCA250.002344332635.21216212
TATTGCG250.002349892235.1951759
CGCCTAT250.002349892235.19517534
ATTGGCG654.620233E-1033.84967821
AGCGGAT607.952622E-932.99547635
AGTCATC353.211322E-431.4242637
ATACTTT353.211322E-431.42426339
GAAGGTA701.02591E-931.42426329
GCCGTCT1350.030.9587243
ATGATCT300.00573125629.33638823
TGAAGGT752.1482265E-929.32931328
GTCCGCG300.005738021329.3293137
ATCGCCT300.005738021329.3293132
GCGCCAT804.256435E-927.50950216
TGCCGGC407.017979E-427.4962342
ATGCCGT4150.027.0324444
GCGGATG756.9630914E-826.39638136
TATGCCG5250.025.9773943