##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5669 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.19774210619157 21.0 12.0 27.0 12.0 34.0 2 20.478920444522842 21.0 11.0 31.0 11.0 34.0 3 20.847592167930852 21.0 11.0 32.0 11.0 34.0 4 21.574351737519844 23.0 11.0 32.0 11.0 34.0 5 20.559710707355794 21.0 11.0 31.0 11.0 34.0 6 21.38295995766449 21.0 11.0 33.0 11.0 37.0 7 22.77615099664844 23.0 11.0 34.0 11.0 37.0 8 21.908802257893807 21.0 11.0 33.0 11.0 37.0 9 22.662197918504145 21.0 12.0 34.0 12.0 37.0 10-11 21.653642617745632 21.0 11.0 33.5 11.0 37.0 12-13 21.717234080084673 21.0 11.0 33.0 11.0 37.0 14-15 21.6345034397601 21.0 11.0 32.0 11.0 37.0 16-17 21.137237608043748 21.0 11.0 31.5 11.0 36.5 18-19 21.41224201799259 21.0 11.0 31.0 11.0 37.0 20-21 21.58634679837714 21.5 11.0 32.0 11.0 37.0 22-23 21.0845828188393 21.0 11.0 31.0 11.0 36.5 24-25 21.22852354912683 21.0 11.0 31.0 11.0 37.0 26-27 20.769712471335332 21.0 11.0 31.0 11.0 36.5 28-29 20.36778973363909 21.0 11.0 31.0 11.0 36.5 30-31 20.366907743870172 21.0 11.0 29.5 11.0 36.0 32-33 20.545687070029988 21.0 11.0 31.0 11.0 36.0 34-35 20.899717763273948 21.0 11.0 31.0 11.0 36.0 36-37 20.76106897159993 21.0 11.0 29.0 11.0 36.0 38-39 20.079820074087138 16.0 11.0 27.0 11.0 36.0 40-41 20.260275180807902 21.0 11.0 27.0 11.0 36.0 42-43 20.561121890986065 21.0 11.0 29.0 11.0 36.0 44-45 20.347680366907746 21.0 11.0 27.0 11.0 36.0 46-47 20.435173751984475 21.0 11.0 29.0 11.0 36.0 48-49 20.641030164050097 21.0 11.0 29.0 11.0 36.0 50-51 20.81689892397248 21.0 11.0 31.0 11.0 36.0 52-53 20.785323690245193 21.0 11.0 31.0 11.0 36.0 54-55 20.81487034750397 21.0 11.0 31.0 11.0 36.0 56-57 20.2342564826248 20.0 11.0 27.0 11.0 36.0 58-59 20.289116246251545 17.5 11.0 29.0 11.0 36.0 60-61 20.417092961721643 20.5 11.0 27.5 11.0 36.0 62-63 20.153466219791852 19.5 11.0 27.5 11.0 36.0 64-65 20.34785676486153 20.0 11.0 29.0 11.0 36.0 66-67 20.796613159287354 20.5 11.0 30.5 11.0 36.0 68-69 20.21441171282413 19.0 11.0 28.0 11.0 36.0 70-71 20.844064208855176 21.0 11.0 31.0 11.0 36.0 72-73 20.263714940906684 19.5 11.0 28.0 11.0 36.0 74-75 19.94417004762745 19.0 11.0 27.5 11.0 34.0 76 18.81107779149762 11.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 3.3624444788052763 1101 2 1.0478749438420252 1101 3 0.5050669911534307 1101 4 0.17520095256978152 1101 5 0.28352634840943125 1101 6 1.1446752044417856 1101 7 4.084444265021098 1101 8 -0.5080884378688673 1101 9 -0.31487639919690835 1101 10-11 -0.9214769535024736 1101 12-13 -0.9623920217232325 1101 14-15 -0.346768127519109 1101 16-17 0.1451156549102386 1101 18-19 -1.7527184788239687 1101 20-21 0.3809517996804104 1101 22-23 -0.39693834564445396 1101 24-25 -0.2623362735362669 1101 26-27 1.1039935187813796 1101 28-29 2.3558518134410242 1101 30-31 1.029421184092648 1101 32-33 1.1935312666349844 1101 34-35 0.8237153480289372 1101 36-37 0.5198952449339451 1101 38-39 0.4638262282907526 1101 40-41 -4.033223538044712 1101 42-43 -0.7307928367755068 1101 44-45 -1.3068295933218756 1101 46-47 -1.301701495811507 1101 48-49 -1.8497874495735864 1101 50-51 3.4109102064719643 1101 52-53 -2.1437891106051445 1101 54-55 -0.30160638086180924 1101 56-57 -1.1065073608768614 1101 58-59 0.384930300008417 1101 60-61 -0.9517621169838257 1101 62-63 0.6915811050608234 1101 64-65 -3.5151845346662647 1101 66-67 -0.7542209234083259 1101 68-69 -0.8413881223718072 1101 70-71 -1.82255234309984 1101 72-73 -2.1370053211639117 1101 74-75 1.3437830470409544 1101 76 -1.9033546276926643 1102 1 -2.0125555211947237 1102 2 4.030017800984883 1102 3 -3.3877901517037117 1102 4 -0.4497990474302185 1102 5 2.5245977769808583 1102 6 -2.9803247955582144 1102 7 -3.424484306407475 1102 8 -4.159874152154583 1102 9 0.02440931508880695 1102 10-11 -0.586655524931043 1102 12-13 0.18492940684819814 1102 14-15 2.144303301052318 1102 16-17 0.05136565491023859 1102 18-19 1.925852949747462 1102 20-21 0.5684517996804104 1102 22-23 4.415561654355546 1102 24-25 -1.8203719878219822 1102 26-27 -1.806720766932905 1102 28-29 -1.6441481865589758 1102 30-31 -0.9750431016216368 1102 32-33 -1.5073615905078732 1102 34-35 -3.3102132233996358 1102 36-37 -2.4309976122089125 1102 38-39 1.879004799719322 1102 40-41 -3.9305449666161394 1102 42-43 -1.5120428367755068 1102 44-45 -5.052365307607591 1102 46-47 -1.7883086386686529 1102 48-49 3.864498264712129 1102 50-51 -0.6515897935280357 1102 52-53 -1.8759319677479986 1102 54-55 -1.2792849522903822 1102 56-57 -3.017221646591146 1102 58-59 1.6215374428655593 1102 60-61 -1.3981906884123951 1102 62-63 0.361223962203681 1102 64-65 -0.5642916775234106 1102 66-67 -5.115828066265468 1102 68-69 -2.5556738366575225 1102 70-71 -3.0234452002426977 1102 72-73 -2.467362464021054 1102 74-75 -0.45085981010190324 1102 76 -3.331926056264093 1103 1 -1.1792221878613915 1103 2 -5.049347278380196 1103 3 4.255066991153431 1103 4 1.4946453970142244 1103 5 2.0960263484094313 1103 6 1.408564093330675 1103 7 3.98027759835443 1103 8 1.3321893399089113 1103 9 0.7545680452475345 1103 10-11 0.4014397131641907 1103 12-13 0.2008024227212104 1103 14-15 0.5490652058142267 1103 16-17 3.27358787713246 1103 18-19 1.2750592989538099 1103 20-21 0.2906740219026318 1103 22-23 -0.8622161234222325 1103 24-25 -0.30053071798070974 1103 26-27 0.8401046298924904 1103 28-29 1.9947407023299135 1103 30-31 1.3384489618704265 1103 32-33 -0.011329844476129836 1103 34-35 1.4834375702511586 1103 36-37 0.0650341338228344 1103 38-39 2.1686873394018633 1103 40-41 4.025804239733066 1103 42-43 0.5712904965578289 1103 44-45 -0.22696848221076493 1103 46-47 0.9934373930773823 1103 48-49 3.316879217093078 1103 50-51 0.6539657620275214 1103 52-53 -0.15767799949403383 1103 54-55 -0.9023008253062521 1103 56-57 -0.03706291643241855 1103 58-59 1.8953469666750813 1103 60-61 0.2287934385717314 1103 62-63 2.0040811050608234 1103 64-65 3.189676576444846 1103 66-67 2.138140187702785 1103 68-69 -1.5913881223718072 1103 70-71 -0.7774134542109508 1103 72-73 0.45327245661386684 1103 74-75 -0.8437169529590456 1103 76 1.5966453723073357 1104 1 0.4249444788052763 1104 2 -4.577125056157975 1104 3 -0.6824330088465693 1104 4 1.1752009525697815 1104 5 -2.9664736515905687 1104 6 -1.0428247955582144 1104 7 -1.915555734978902 1104 8 0.9294115621311327 1104 9 -0.5648763991969084 1104 10-11 0.5785230464975264 1104 12-13 2.1313579782767675 1104 14-15 1.278231872480891 1104 16-17 -0.1673843450897614 1104 18-19 -2.6277184788239687 1104 20-21 1.0372017996804104 1104 22-23 -0.02193834564445396 1104 24-25 3.175163726463733 1104 26-27 0.13524351878137963 1104 28-29 0.8246018134410242 1104 30-31 2.810671184092648 1104 32-33 1.1935312666349844 1104 34-35 0.1674653480289372 1104 36-37 -2.167604755066055 1104 38-39 -1.0361737717092474 1104 40-41 0.40427646195528766 1104 42-43 1.7067071632244932 1104 44-45 -1.5255795933218756 1104 46-47 1.635798504188493 1104 48-49 -1.8810374495735864 1104 50-51 -2.4953397935280357 1104 52-53 -0.05003911060514454 1104 54-55 0.7608936191381908 1104 56-57 -0.04400736087686141 1104 58-59 -0.552569699991583 1104 60-61 -2.0455121169838257 1104 62-63 1.7540811050608234 1104 64-65 -0.04643453466626468 1104 66-67 -0.06672092340832592 1104 68-69 -2.372638122371807 1104 70-71 0.48994765690015996 1104 72-73 0.36299467883608827 1104 74-75 0.7500330470409544 1104 76 -1.4658546276926643 1105 1 0.32077781213860845 1105 2 -0.8033155323484493 1105 3 -1.0782663421799015 1105 4 -0.02122761885879143 1105 5 -1.8325450801619958 1105 6 -1.432705747939167 1105 7 -2.138770020693187 1105 8 0.6258401335597057 1105 9 1.3339331246126171 1105 10-11 1.4252492369737162 1105 12-13 1.9349294068481981 1105 14-15 0.14430330105231803 1105 16-17 -0.5914914879469038 1105 18-19 1.5687100926046007 1105 20-21 2.925594656823268 1105 22-23 1.3798473686412613 1105 24-25 -0.5584672259172194 1105 26-27 2.217088756876617 1105 28-29 2.8439470515362615 1105 30-31 0.14400451742598364 1105 32-33 -0.23355206669835127 1105 34-35 1.2612153480289372 1105 36-37 1.8070976258863247 1105 38-39 -0.4662332955187729 1105 40-41 0.31945503338385706 1105 42-43 0.9046238298911611 1105 44-45 1.947634692392409 1105 46-47 1.1164532660932558 1105 48-49 -0.5045493543354915 1105 50-51 2.0626959207576796 1105 52-53 1.183591841775808 1105 54-55 0.8992864762810484 1105 56-57 0.9708735915040911 1105 58-59 0.12153744286555934 1105 60-61 0.35180931158760487 1105 62-63 -0.2697284187487021 1105 64-65 1.9952321320003996 1105 66-67 0.6460766956392945 1105 68-69 1.2538499728662877 1105 70-71 -0.9639213907188875 1105 72-73 -0.9078386544972474 1105 74-75 0.4062830470409544 1105 76 0.9299787056406679 1106 1 1.5207778121386113 1106 2 -5.193791722824642 1106 3 -3.411599675513237 1106 4 -4.649799047430218 1106 5 -0.9373069849239037 1106 6 1.9863418711084506 1106 7 -3.719722401645569 1106 8 3.4544115621311313 1106 9 0.8101236008030916 1106 10-11 -1.1652269535024722 1106 12-13 -0.0269753550565639 1106 14-15 1.4490652058142253 1106 16-17 -0.1486343450897607 1106 18-19 -0.06938514549063513 1106 20-21 0.1351184663470768 1106 22-23 -0.8677716789777854 1106 24-25 -3.2060862735362683 1106 26-27 0.045660185448046064 1106 28-29 0.7725184801076921 1106 30-31 0.31067118409264793 1106 32-33 -0.30021873336501415 1106 34-35 -3.3721179853043957 1106 36-37 -2.3738547550660556 1106 38-39 -2.409090438375916 1106 40-41 -2.651973538044711 1106 42-43 -0.5287095034421725 1106 44-45 1.628587073344793 1106 46-47 -2.6121181624781755 1106 48-49 0.03354588375974643 1106 50-51 -1.7182564601947021 1106 52-53 -1.6354557772718117 1106 54-55 -2.7578563808618064 1106 56-57 -0.6815073608768607 1106 58-59 0.3120136333417527 1106 60-61 0.5565712163495071 1106 62-63 0.7207477717274919 1106 64-65 0.4952321320003996 1106 66-67 -1.972970923408326 1106 68-69 1.4252785442948586 1106 70-71 -3.9496356764331715 1106 72-73 -1.1745053211639132 1106 74-75 -0.7603836196257099 1106 76 -1.6033546276926636 1107 1 3.040076057752646 1107 2 3.2518223122630765 1107 3 -1.4817751141097268 1107 4 -1.9761148369039034 1107 5 0.17497371683048257 1107 6 0.5723067833891555 1107 7 1.146944265021098 1107 8 -1.366641069447816 1107 9 -0.610929030775857 1107 10-11 -0.37049011139721344 1107 12-13 -1.8620630743548112 1107 14-15 -1.6263733906770064 1107 16-17 -1.2381080293002888 1107 18-19 0.6288604685444525 1107 20-21 -0.40523241084590467 1107 22-23 -1.4133857140655088 1107 24-25 -2.053454694588897 1107 26-27 -0.4946906917449354 1107 28-29 2.3953254976515517 1107 30-31 2.0475132893558055 1107 32-33 -0.03179768073343325 1107 34-35 0.18226797960788232 1107 36-37 -0.7317494919081611 1107 38-39 -0.8897921927618775 1107 40-41 -0.04144722225523978 1107 42-43 0.6590097948034419 1107 44-45 -0.8117638038481907 1107 46-47 0.907180083135863 1107 48-49 -0.09978744957358643 1107 50-51 1.0063049433140705 1107 52-53 0.8101582578159068 1107 54-55 -2.984172170335494 1107 56-57 -3.4025599924558065 1107 58-59 -1.3897407526231618 1107 60-61 0.5285010409109105 1107 62-63 -1.535392579149704 1107 64-65 -0.43459242940310716 1107 66-67 -1.1308656602504286 1107 68-69 -1.6308618065823346 1107 70-71 -0.942618132573525 1107 72-73 -1.2587158474796993 1107 74-75 -1.409506426643258 1107 76 1.1229611617810207 1108 1 -1.4336081527736724 1108 2 -4.011335582473766 1108 3 -0.2712487983202543 1108 4 -0.8182201000617972 1108 5 -6.193447335801094 1108 6 -3.848745848189793 1108 7 -3.800424156031532 1108 8 -0.7876937010267611 1108 9 3.8101236008030916 1108 10-11 -3.054700637713001 1108 12-13 -3.809431495407445 1108 14-15 -1.9947944433085816 1108 16-17 -3.738108029300289 1108 18-19 -4.292192163034496 1108 20-21 -2.4841797792669595 1108 22-23 -1.4133857140655088 1108 24-25 -0.2376652209046881 1108 26-27 -3.363111744376514 1108 28-29 -0.6309902918221333 1108 30-31 -2.8209077632757733 1108 32-33 -4.268639785996594 1108 34-35 -4.52825833618159 1108 36-37 -4.415960018223952 1108 38-39 -3.363476403288196 1108 40-41 -2.2519735380447123 1108 42-43 -5.104148099933401 1108 44-45 -1.4696585406902933 1108 46-47 -2.724398864232562 1108 48-49 -0.836629554836744 1108 50-51 -3.6515897935280357 1108 52-53 -1.6108943737630383 1108 54-55 -0.5368037492828641 1108 56-57 -2.639402097718964 1108 58-59 -2.468688121044213 1108 60-61 -3.9714989590890895 1108 62-63 -1.7985504738865465 1108 64-65 -1.987224008350477 1108 66-67 -1.3413919760399047 1108 68-69 -3.236124964477071 1108 70-71 -3.36367076415247 1108 72-73 -0.9692421632691754 1108 74-75 -3.4358222161169394 1108 76 0.12296116178102068 1109 1 1.9874444788052763 1109 2 -0.9521250561579748 1109 3 2.4425669911534307 1109 4 -1.3872990474302185 1109 5 0.40852634840943125 1109 6 0.3321752044417856 1109 7 -3.103055734978902 1109 8 0.36691156213113274 1109 9 -2.3773763991969084 1109 10-11 0.3597730464975264 1109 12-13 1.6001079782767675 1109 14-15 -3.909268127519109 1109 16-17 -1.3861343450897614 1109 18-19 -1.5027184788239687 1109 20-21 -0.2752982003195896 1109 22-23 -0.02193834564445396 1109 24-25 0.5501637264637331 1109 26-27 -0.6772564812186204 1109 28-29 -0.8941481865589758 1109 30-31 -0.033078815907352066 1109 32-33 -0.8064687333650156 1109 34-35 -2.238784651971063 1109 36-37 -0.3551047550660549 1109 38-39 2.0888262282907526 1109 40-41 -3.2207235380447123 1109 42-43 -0.7932928367755068 1109 44-45 1.3181704066781244 1109 46-47 -0.645451495811507 1109 48-49 -1.6622874495735864 1109 50-51 -0.1828397935280357 1109 52-53 0.38746088939485546 1109 54-55 -0.5828563808618092 1109 56-57 -1.7627573608768614 1109 58-59 0.603680300008417 1109 60-61 -1.4205121169838257 1109 62-63 -0.4021688949391766 1109 64-65 -1.8276845346662647 1109 66-67 0.15202907659167408 1109 68-69 1.1586118776281928 1109 70-71 2.02119765690016 1109 72-73 2.4567446788360883 1109 74-75 0.2812830470409544 1109 76 -1.0283546276926643 1110 1 -1.8360849329594302 1110 2 -1.2094779973344458 1110 3 0.13741993232990168 1110 4 0.7854950702168395 1110 5 -0.8157383574729202 1110 6 -5.42150126614645 1110 7 -3.617761617331844 1110 8 -1.9455884378688673 1110 9 -3.3075234580204373 1110 10-11 -1.4122857770318866 1110 12-13 0.38871091945323855 1110 14-15 -3.343091656930877 1110 16-17 -3.3309872862662324 1110 18-19 -2.6497773023533817 1110 20-21 -1.9315482003195896 1110 22-23 -1.0403206985856315 1110 24-25 -1.1649098029480314 1110 26-27 -2.3445358929833233 1110 28-29 -3.3059128924413272 1110 30-31 -0.18932881590735207 1110 32-33 -3.0178657921885446 1110 34-35 -3.474078769618121 1110 36-37 -2.8973841668307614 1110 38-39 -1.648306124650425 1110 40-41 -1.3402088321623573 1110 42-43 -2.40910166030492 1110 44-45 -2.014550181557169 1110 46-47 -1.851333848752681 1110 48-49 -1.393905096632409 1110 50-51 0.9660572652954933 1110 52-53 -0.6217302870757315 1110 54-55 -0.22256226321475125 1110 56-57 -0.24621324322980342 1110 58-59 -1.109554994109228 1110 60-61 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-0.012555521194723696 2117 2 -0.4699821990151172 2117 3 -3.1735044374179964 2117 4 1.3359152382840662 2117 5 1.881740634123716 2117 6 -2.1946105098439297 2117 7 1.2183728364496709 2117 8 2.8401258478454174 2117 9 1.8815521722316646 2117 10-11 0.7704873322118111 2117 12-13 -0.24364202172323246 2117 14-15 -3.2485538418048243 2117 16-17 -1.5200629165183308 2117 18-19 1.3187100926046007 2117 20-21 0.5684517996804104 2117 22-23 -1.834438345644454 2117 24-25 -1.1775148449648363 2117 26-27 -1.699577909790051 2117 28-29 -1.608433900844691 2117 30-31 0.38209975552121733 2117 32-33 2.492638409492127 2117 34-35 1.7612153480289372 2117 36-37 1.4618595306482298 2117 38-39 0.2718619425764679 2117 40-41 -2.3948306809018547 2117 42-43 -0.6906142653469374 2117 44-45 3.0190632638209784 2117 46-47 1.854548504188493 2117 48-49 -0.9212160210021558 2117 50-51 -1.0801612220994663 2117 52-53 0.26692517510914016 2117 54-55 -0.02928495229037864 2117 56-57 1.1970640676945692 2117 58-59 -1.021319699991583 2117 60-61 -2.8267621169838257 2117 62-63 -1.817347466367746 2117 64-65 -0.31429167752340703 2117 66-67 0.09845764802024348 2117 68-69 0.3014690204853352 2117 70-71 -0.9877309145284094 2117 72-73 -2.217362464021054 2117 74-75 -2.3794312386733303 2117 76 -2.760497484835522 2118 1 -0.8125555211947244 2118 2 -2.193791722824642 2118 3 2.6550669911534293 2118 4 0.7502009525697808 2118 5 -1.8039736515905673 2118 6 -0.013658128891549381 2118 7 -1.386389068312237 2118 8 -3.6789217712022015 2118 9 -1.0565430658635755 2118 10-11 -1.6985602868358072 2118 12-13 1.7063579782767704 2118 14-15 -0.8176014608524405 2118 16-17 -2.0819676784230943 2118 18-19 -2.236051812157303 2118 20-21 -2.2315482003195903 2118 22-23 -2.001105012311122 2118 24-25 0.22724705979706883 2118 26-27 2.145660185448044 2118 28-29 0.6391851467743557 2118 30-31 -3.0893288159073506 2118 32-33 -1.5668854000316834 2118 34-35 -0.6387846519710614 2118 36-37 0.5261452449339465 2118 38-39 -1.1424237717092467 2118 40-41 -1.3853068713780452 2118 42-43 -3.7620428367755068 2118 44-45 2.061920406678123 2118 46-47 -0.645451495811507 2118 48-49 -3.199787449573588 2118 50-51 -2.0849231268613693 2118 52-53 -0.002122443938478824 2118 54-55 2.075476952471522 2118 56-57 0.15182597245647145 2118 58-59 -2.4213196999915816 2118 60-61 1.5899045496828386 2118 62-63 -0.6459188949391752 2118 64-65 -0.904767867999599 2118 66-67 -2.5396375900749923 2118 68-69 -0.7747214557051407 2118 70-71 0.017030990233493526 2118 72-73 -2.0745053211639117 2118 74-75 -1.9603836196257127 2118 76 -0.9366879610259993 2119 1 3.805626296987093 2119 2 1.3092385802056619 2119 3 3.3459760820625206 2119 4 3.732019134387965 2119 5 0.9596627120457946 2119 6 4.156038840805422 2119 7 3.9651260832029145 2119 8 4.327138834858406 2119 9 4.901032691712182 2119 10-11 2.5075003192247998 2119 12-13 0.09726706918585748 2119 14-15 0.39755005429907087 2119 16-17 1.824092927637512 2119 18-19 5.815463339357851 2119 20-21 3.7502699814985903 2119 22-23 2.5746525634464525 2119 24-25 2.393913726463733 2119 26-27 4.433538973326833 2119 28-29 3.0149427225319307 2119 30-31 4.037943911365375 2119 32-33 6.508872175725891 2119 34-35 -2.011511924698336 2119 36-37 1.5625088812975818 2119 38-39 -0.21515104443652078 2119 40-41 0.11166282559165097 2119 42-43 -0.8074973822300535 2119 44-45 -2.3835341387764224 2119 46-47 0.08182123146121967 2119 48-49 2.2183943686082337 2119 50-51 3.3484102064719643 2119 52-53 2.322119980303949 2119 54-55 0.31487089186546413 2119 56-57 1.291219911850412 2119 58-59 3.5241348454629637 2119 60-61 2.2868742466525376 2119 62-63 1.3449901959699169 2119 64-65 4.464929101697372 2119 66-67 5.390665440228037 2119 68-69 1.8858846049009195 2119 70-71 3.538243111445613 2119 72-73 1.1982219515633616 2119 74-75 3.2244648652227745 2119 76 -0.6760819004199377 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 10.0 13 96.0 14 260.0 15 371.0 16 493.0 17 599.0 18 677.0 19 665.0 20 567.0 21 444.0 22 322.0 23 181.0 24 168.0 25 107.0 26 81.0 27 60.0 28 58.0 29 29.0 30 39.0 31 29.0 32 35.0 33 49.0 34 49.0 35 73.0 36 93.0 37 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.675128705840583 28.084502041540922 25.492632700159774 36.747736552458726 2 3.757276415593579 42.45898747574528 28.8763450343976 24.907391074263536 3 4.8509437290527435 47.36285059093315 21.820426883048157 25.965778796965957 4 4.127712118539425 30.34044805080261 22.17322279061563 43.35861704004233 5 4.233550890809667 35.526547892044455 31.76927147645087 28.47062974069501 6 6.950079379079202 33.78020814958547 26.001058387722704 33.26865408361263 7 5.309578408890457 47.64508731698712 13.476803669077439 33.568530605044984 8 5.203739636620215 39.56606103369201 23.866643146939495 31.36355618274828 9 7.920268124889751 33.14517551596401 16.387369906509083 42.54718645263715 10-11 7.4087140589169165 33.542070911977426 25.74528135473628 33.30393367436938 12-13 7.249955900511554 29.828893984829776 24.933850767331098 37.98729934732757 14-15 10.416299170929618 33.39213265126125 18.901040747927325 37.29052742988181 16-17 13.64438172517199 31.89275004409949 20.012347856764862 34.45052037396366 18-19 10.469218557064739 30.843182219086255 18.52178514729229 40.165814076556714 20-21 10.336920091726936 30.737343446816016 18.76874228258952 40.15699417886753 22-23 15.187863820779679 30.375727641559358 17.798553536778975 36.63785500088199 24-25 11.84512259657788 34.82095607690951 16.528488269536073 36.80543305697654 26-27 10.6279767154701 35.08555300758511 20.162286117481038 34.12418415946375 28-29 12.86823072852355 31.46939495501852 23.319809490209913 32.342564826248015 30-31 12.929970012347859 31.06367966131593 23.751984476980066 32.25436584935615 32-33 9.957664491091903 35.18257188216617 22.83471511730464 32.02504850943729 34-35 9.798906332686542 36.31151878638208 20.42688304815664 33.46269183277474 36-37 10.848474157699771 31.663432704180632 22.464279414358792 35.0238137237608 38-39 11.836302698888693 26.080437466925382 27.394602222614218 34.688657611571706 40-41 10.18698183101076 28.69112718292468 24.96031046039866 36.161580525665904 42-43 6.535544187687423 25.207267595695892 28.00317516316811 40.25401305344858 44-45 7.320515082025049 26.644910919033336 30.058211324748633 35.97636267419298 46-47 8.202504850943729 30.2787087669783 27.65919915328982 33.85958722878814 48-49 7.576292115011466 33.45387193508556 22.993473275710002 35.97636267419298 50-51 7.523372728876344 32.99523725524784 19.90650908449462 39.574880931381195 52-53 6.226847768565884 33.24219439054507 20.48862233198095 40.042335508908096 54-55 7.055918151349444 30.790262832951136 20.85023813723761 41.30358087846181 56-57 10.530957840889045 31.81337096489681 19.809490209913566 37.84618098430058 58-59 9.481390015875816 31.160698535896987 18.433586170400424 40.92432527782678 60-61 7.391074263538543 29.14976186276239 20.603281001940378 42.85588287175869 62-63 8.828717586875992 29.96119245016758 19.950608572940553 41.25948139001588 64-65 9.3579114482272 30.66678426530252 16.599047451049568 43.376256835420705 66-67 7.514552831187158 36.67313459163874 13.732580702063856 42.07973187511025 68-69 9.260892573646146 36.57611571705768 14.588110777914975 39.574880931381195 70-71 7.294055388957488 40.14817428117834 13.221026636091022 39.33674369377315 72-73 9.57840889045687 37.572764155935786 14.200035279590756 38.64879167401658 74-75 8.978655847592169 37.62568354207092 15.73469747750926 37.66096313282766 76 10.319280296348563 35.93226318574704 16.581407655671192 37.1670488622332 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.5 12 3.0 13 1.5 14 0.5 15 1.5 16 3.0 17 4.0 18 2.0 19 2.5 20 5.0 21 5.0 22 7.0 23 12.0 24 18.0 25 21.0 26 27.5 27 37.0 28 44.0 29 48.0 30 63.5 31 94.0 32 104.0 33 99.0 34 113.5 35 144.5 36 178.5 37 196.0 38 201.0 39 226.0 40 274.5 41 285.5 42 268.0 43 310.5 44 347.5 45 362.0 46 382.0 47 374.0 48 353.0 49 313.0 50 286.0 51 269.5 52 239.0 53 208.0 54 191.0 55 183.5 56 167.5 57 137.0 58 115.0 59 116.5 60 99.5 61 73.0 62 65.0 63 61.5 64 51.0 65 35.0 66 25.0 67 24.0 68 23.5 69 14.5 70 6.0 71 6.0 72 8.0 73 7.5 74 6.0 75 7.0 76 5.0 77 1.5 78 1.5 79 3.0 80 3.5 81 3.0 82 1.0 83 0.0 84 0.0 85 1.5 86 1.5 87 0.0 88 0.5 89 0.5 90 1.5 91 2.5 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.63503263362145 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 5669.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.28347151173047 #Duplication Level Percentage of deduplicated Percentage of total 1 98.36808703535812 95.69588992767683 2 0.942883046237534 1.8345387193508553 3 0.43517679057116954 1.2700652672429 4 0.14505893019038985 0.5644734521079555 5 0.03626473254759746 0.17639795378373613 6 0.03626473254759746 0.21167754454048332 7 0.03626473254759746 0.24695713529723057 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGTGAGGCAGGAATGAAAAGTCAGGCAGAGAGGGTGTGGTAACTAACCA 7 0.12347856764861528 No Hit CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG 7 0.12347856764861528 No Hit CTTATACACATCTGACGCCTGTCTCTTATACACATCTGACGCTGCCGACG 6 0.10583877227024166 No Hit TCATAGTACTAGTAAGTGGCAGATTAAGGGTTTTGATTTTATATGTTTCT 6 0.10583877227024166 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1763979537837361 0.0 2 0.0 0.0 0.0 0.1763979537837361 0.0 3 0.0 0.0 0.0 0.1763979537837361 0.0 4 0.0 0.0 0.0 0.1763979537837361 0.0 5 0.0 0.0 0.0 0.21167754454048332 0.0 6 0.0 0.0 0.0 0.21167754454048332 0.0 7 0.0 0.0 0.0 0.21167754454048332 0.0 8 0.0 0.0 0.0 0.21167754454048332 0.0 9 0.0 0.0 0.0 0.21167754454048332 0.0 10 0.0 0.0 0.0 0.21167754454048332 0.0 11 0.0 0.0 0.0 0.21167754454048332 0.0 12 0.0 0.0 0.0 0.26459693067560414 0.0 13 0.0 0.0 0.0 0.317516316810725 0.0 14 0.0 0.0 0.0 0.3527959075674722 0.0 15 0.0 0.0 0.0 0.44099488445934026 0.0 16 0.0 0.0 0.0 0.4586346798377139 0.0 17 0.0 0.0 0.0 0.4939142705944611 0.0 18 0.0 0.0 0.0 0.5115540659728347 0.0 19 0.0 0.0 0.0 0.7585112012700652 0.0 20 0.0 0.0 0.0 0.8290703827835597 0.0 21 0.0 0.0 0.0 0.8467101781619333 0.0 22 0.0 0.0 0.0 0.864349973540307 0.0 23 0.0 0.0 0.0 0.8996295642970542 0.0 24 0.0 0.0 0.0 0.8996295642970542 0.0 25 0.0 0.0 0.0 0.8996295642970542 0.0 26 0.0 0.0 0.0 0.8996295642970542 0.0 27 0.0 0.0 0.0 0.8996295642970542 0.0 28 0.0 0.0 0.0 0.9172693596754278 0.0 29 0.0 0.0 0.0 0.9172693596754278 0.0 30 0.0 0.0 0.0 0.952548950432175 0.0 31 0.0 0.0 0.0 1.0760275180807903 0.0 32 0.0 0.0 0.0 1.128946904215911 0.0 33 0.0 0.0 0.0 1.2524254718645265 0.0 34 0.0 0.0 0.0 1.3053448579996472 0.0 35 0.0 0.0 0.0 1.3935438348915152 0.0 36 0.0 0.0 0.0 1.4288234256482626 0.0 37 0.0 0.0 0.0 1.6581407655671194 0.0 38 0.0 0.0 0.0 1.763979537837361 0.0 39 0.0 0.0 0.0 2.0991356500264597 0.0 40 0.0 0.0 0.0 2.310813194566943 0.0 41 0.0 0.0 0.0 2.5577703298641734 0.0 42 0.0 0.0 0.0 2.593049920620921 0.0 43 0.0 0.0 0.0 2.6636091021344153 0.0 44 0.0 0.0 0.0 2.9634856235667666 0.0 45 0.0 0.0 0.0 3.351561121890986 0.0 46 0.0 0.0 0.0 3.527959075674722 0.0 47 0.0 0.0 0.0 3.686717234080085 0.0 48 0.0 0.0 0.0 3.8807549832421944 0.0 49 0.0 0.0 0.0 4.039513141647557 0.0 50 0.0 0.0 0.0 4.180631504674546 0.0 51 0.0 0.0 0.0 4.321749867701534 0.0 52 0.0 0.0 0.0 4.603986593755512 0.0 53 0.0 0.0 0.0 4.6745457752690065 0.0 54 0.0 0.0 0.0 4.798024342917622 0.0 55 0.0 0.0 0.0 4.992062092079732 0.0 56 0.0 0.0 0.0 5.062621273593226 0.0 57 0.0 0.0 0.0 5.0802610689716 0.0 58 0.0 0.0 0.0 5.133180455106721 0.0 59 0.0 0.0 0.0 5.291938613512083 0.0 60 0.0 0.0 0.0 5.380137590403951 0.0 61 0.0 0.0 0.0 5.468336567295819 0.0 62 0.0 0.0 0.0 5.574175339566061 0.0 63 0.0 0.0 0.0 5.732933497971423 0.0 64 0.0 0.0 0.0 5.785852884106545 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACATC 75 0.0016192041 23.330381 6 >>END_MODULE