FastQCFastQC Report
Wed 25 May 2016
SRR1780680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5669
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT221739.107426353854294No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT5349.419650732051508RNA PCR Primer, Index 15 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2734.815664138295996No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2304.05715293702593No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1051.8521785147292291No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1021.7992591285941082No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT560.9878285411889223No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC500.8819897689186805No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT490.8643499735403068No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT460.8114305874051861No Hit
CTTATACACATCTCCGAGCCCACGAGCCATAAGTTAATCTCGTATGCCGT250.44099488445934026No Hit
CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT220.38807549832421945No Hit
CTTATACACATCTCCGAGCCCACGCGACATAAGTTAATCTCGTATGCCGT220.38807549832421945No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCG200.35279590756747226No Hit
ACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCT190.3351561121890986RNA PCR Primer, Index 15 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT190.3351561121890986No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCC190.3351561121890986No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT180.317516316810725No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGTCTT170.2998765214323514No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC170.2998765214323514No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCG170.2998765214323514No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTAGTATGCCGT170.2998765214323514No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCC160.28223672605397776No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT150.2645969306756042No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT140.24695713529723057No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGTCTT130.22931733991885692No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC130.22931733991885692No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTCATCTCGTATGCCGT120.21167754454048332No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG110.19403774916210972No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT110.19403774916210972No Hit
CTTATACACATCTACGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT110.19403774916210972No Hit
ATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTGCTT110.19403774916210972Illumina PCR Primer Index 8 (96% over 26bp)
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG100.17639795378373613No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG100.17639795378373613No Hit
CTTATACACATCTCCGAGCCCAAGAGACATAAGTTAATCTCGTATGCCGT100.17639795378373613No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTACTCTCGTATGCCGT100.17639795378373613No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCC90.1587581584053625No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG90.1587581584053625No Hit
CTTATAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG90.1587581584053625No Hit
CTTATACACATCTCCGAGCACACGAGACATAAGTTAATCTCGTATGCCGT90.1587581584053625No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGTCTT80.14111836302698888No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGC80.14111836302698888No Hit
AATGTGGTTAGTTACCACACCCTCTCTGCCTGACTTTTCATTCCTGCCTC80.14111836302698888No Hit
ATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGTCTT80.14111836302698888No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTCTGCCGT80.14111836302698888No Hit
TCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT80.14111836302698888No Hit
CTTATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT80.14111836302698888No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCACGTATGCCGT70.12347856764861528No Hit
ATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGTCTT70.12347856764861528RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCCCGTATGCCGT70.12347856764861528No Hit
CTTATACACATCTCCGAGCCAACGAGACATAAGTTAATCTCGTATGCCGT70.12347856764861528No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAAATCGTATGCCGT70.12347856764861528No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTCTGCCGTCTT70.12347856764861528No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC70.12347856764861528No Hit
CATTATAATAAGGGCTTTTGTGTCAGAAACATATAAAATCAAAACCCTTA60.10583877227024166No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCG60.10583877227024166No Hit
CTTATACACATATCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT60.10583877227024166No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATTTCGTATGCCGT60.10583877227024166No Hit
GGGCTGAATGTCCACGTTCTGCCTGTGGACAGGGCCTCGGGTGAGAGCTG60.10583877227024166No Hit
CTTATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG60.10583877227024166No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACAA150.002111759770.0000170
CTTTACA252.4919646E-670.01
TTTACAC252.4919646E-670.02
TTACACA351.833356E-550.03
AACAACA305.394139E-446.66666869
CTTATAC3650.046.0273971
TATACAC3800.044.210533
TTATACA3800.044.210532
CTCTTAT752.5975169E-837.3333321
ACTCGTA400.002222021635.038
AAACTCG400.002222021635.036
TAAACTC400.002222021635.035
AAACAAC501.5051136E-435.068
AACTCGT400.002222021635.037
TTAAACT400.002222021635.034
GTTAAAC400.002222021635.033
ATCTCGT4100.034.14634337
AATCTCG4000.034.12536
TAATCTC4000.034.12535
GAAAAAA4750.033.89473760
Produced by FastQC (version 0.11.5)