##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780680_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5669 Sequences flagged as poor quality 0 Sequence length 76 %GC 40 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.639618980419826 34.0 34.0 34.0 31.0 34.0 2 33.19139177985535 34.0 34.0 34.0 32.0 34.0 3 33.3861351208326 34.0 34.0 34.0 33.0 34.0 4 33.50379255600635 34.0 34.0 34.0 34.0 34.0 5 33.54895043217498 34.0 34.0 34.0 34.0 34.0 6 37.37678602928206 38.0 38.0 38.0 37.0 38.0 7 36.895043217498674 38.0 37.0 38.0 35.0 38.0 8 36.95837008290704 38.0 37.0 38.0 35.0 38.0 9 36.73981301816899 38.0 37.0 38.0 34.0 38.0 10-11 36.4685129652496 38.0 37.0 38.0 34.0 38.0 12-13 36.11906861880402 38.0 37.0 38.0 33.5 38.0 14-15 36.24810372199683 38.0 37.0 38.0 33.0 38.0 16-17 36.088551772799434 38.0 36.0 38.0 32.5 38.0 18-19 36.02319633092256 38.0 36.5 38.0 32.5 38.0 20-21 35.83065796436762 38.0 36.0 38.0 31.5 38.0 22-23 36.18724642794143 38.0 36.5 38.0 32.5 38.0 24-25 34.797495149056274 37.0 34.0 38.0 28.0 38.0 26-27 34.9381725171988 37.0 34.0 38.0 30.0 38.0 28-29 35.00414535191392 37.0 35.5 38.0 29.0 38.0 30-31 34.683542070911976 37.0 34.5 38.0 27.0 38.0 32-33 34.9761862762392 37.0 35.5 38.0 27.0 38.0 34-35 34.47636267419298 37.0 34.0 38.0 27.0 38.0 36-37 34.76283295113777 37.0 34.5 38.0 27.0 38.0 38-39 30.446286823072853 34.0 27.0 37.0 11.0 38.0 40-41 32.93737872640678 36.5 32.5 37.5 18.5 38.0 42-43 34.90589169165638 37.0 34.5 38.0 29.0 38.0 44-45 35.444081848650555 37.0 36.0 38.0 31.0 38.0 46-47 35.60169342035633 38.0 36.0 38.0 31.0 38.0 48-49 35.16510848474158 37.5 35.0 38.0 29.0 38.0 50-51 34.992062092079735 37.0 34.5 38.0 29.0 38.0 52-53 34.54065972834715 37.0 34.0 38.0 26.5 38.0 54-55 35.07602751808079 37.0 35.5 38.0 29.0 38.0 56-57 35.0792026812489 37.5 35.5 38.0 27.0 38.0 58-59 34.60336920091727 37.0 34.5 38.0 26.5 38.0 60-61 34.44637502204974 37.0 34.5 38.0 25.5 38.0 62-63 35.18292467807373 37.0 35.5 38.0 29.0 38.0 64-65 35.24678073734344 37.5 36.0 38.0 29.0 38.0 66-67 33.240077615099665 37.0 32.0 38.0 22.0 38.0 68-69 30.37590403951314 34.5 24.0 37.5 11.0 38.0 70-71 26.668724642794142 29.0 17.0 35.5 10.5 37.5 72-73 21.485006173928383 22.0 11.0 29.0 10.5 36.5 74-75 20.724642794143588 21.0 11.0 27.5 11.0 36.0 76 21.142353148703474 21.0 11.0 28.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.25552628542586575 1101 2 0.7012977520686903 1101 3 0.03923753563438481 1101 4 0.3707133805940188 1101 5 0.42166414992408363 1101 6 -1.8907900656953558 1101 7 0.41158647198039233 1101 8 0.7758118956342841 1101 9 1.03611325058084 1101 10-11 1.1926897102476488 1101 12-13 0.6243985736530888 1101 14-15 0.5289356960119349 1101 16-17 -1.5347302970077834 1101 18-19 -1.8405314678351203 1101 20-21 0.7874975970248528 1101 22-23 0.9312985236049158 1101 24-25 -0.8926073290766965 1101 26-27 -1.356510905652371 1101 28-29 1.0498030214336183 1101 30-31 1.6876319728715856 1101 32-33 0.4110642080476623 1101 34-35 1.0052586165237898 1101 36-37 0.8810772288901845 1101 38-39 1.6152171807968614 1101 40-41 2.8279030512129992 1101 42-43 0.6896828398142247 1101 44-45 -0.08640384106460886 1101 46-47 -0.43543617861688233 1101 48-49 -0.38407429502223067 1101 50-51 -4.519004858027387 1101 52-53 -3.172211861122868 1101 54-55 -3.0122496715869858 1101 56-57 -5.598420093351962 1101 58-59 -2.090062970372472 1101 60-61 -2.1315560867739336 1101 62-63 -2.5187763416697635 1101 64-65 -2.7320729428538684 1101 66-67 -1.8515051049040423 1101 68-69 -2.852283318699122 1101 70-71 -1.0699164080605534 1101 72-73 0.05741097465983458 1101 74-75 0.9510396539192172 1101 76 -3.1551342461966563 1102 1 -1.6215977139972928 1102 2 0.11201203778297497 1102 3 0.15530896420581541 1102 4 0.4867848091654494 1102 5 -0.0069072786473469705 1102 6 0.4574242200189289 1102 7 0.16158647198039233 1102 8 0.8115261813485688 1102 9 1.0896846791522705 1102 10-11 0.9159039959619335 1102 12-13 0.9011842879388041 1102 14-15 1.1137571245833655 1102 16-17 0.7956268458493554 1102 18-19 0.29339710359344906 1102 20-21 1.8589261684534222 1102 22-23 1.2214770950334852 1102 24-25 -5.343500186219551 1102 26-27 -1.731510905652371 1102 28-29 0.8221244500050489 1102 30-31 1.2412034014430162 1102 32-33 -0.0978643633809071 1102 34-35 1.8088300450952204 1102 36-37 -4.636779913966958 1102 38-39 -0.996389962060281 1102 40-41 3.3546887654987145 1102 42-43 1.4932542683856553 1102 44-45 1.900203301792537 1102 46-47 1.8190281070974024 1102 48-49 -4.2680028664508 1102 50-51 1.3068879991154745 1102 52-53 2.1179667103057014 1102 54-55 1.786857471270153 1102 56-57 1.593544192362323 1102 58-59 1.3161870296275282 1102 60-61 -7.301198943916788 1102 62-63 -2.5321691988126247 1102 64-65 1.1741770571461316 1102 66-67 3.5145663236673883 1102 68-69 -2.352283318699122 1102 70-71 -0.9449164080605534 1102 72-73 -3.996160453911596 1102 74-75 -4.856996060366498 1102 76 -1.7265628176252292 1103 1 1.3069737145741342 1103 2 0.7707421965131331 1103 3 -0.4954846865878366 1103 4 0.44710226948290455 1103 5 -1.1894469611870306 1103 6 0.3383766009713085 1103 7 0.9393642497581709 1103 8 0.6369230067453984 1103 9 0.6611132505808399 1103 10-11 0.07463415469209167 1103 12-13 -1.3408792041246897 1103 14-15 0.740741251567492 1103 16-17 -0.4305636303411191 1103 18-19 0.6386351988315511 1103 20-21 -0.9625024029751472 1103 22-23 0.955604079160473 1103 24-25 -2.9863573290766965 1103 26-27 -0.8981775723190424 1103 28-29 -2.523113645233053 1103 30-31 -1.2429235826839715 1103 32-33 1.0846753191587766 1103 34-35 -2.758630272365096 1103 36-37 -1.1050338822209298 1103 38-39 -0.8813105969809172 1103 40-41 0.5848474956574421 1103 42-43 0.2591272842586676 1103 44-45 0.9795683811576126 1103 46-47 0.9777582658275605 1103 48-49 -3.1965742950222307 1103 50-51 -0.27247708024961526 1103 52-53 0.5187603610993534 1103 54-55 -0.3837774493647643 1103 56-57 0.2522743510924812 1103 58-59 0.17729814073864247 1103 60-61 -5.832944975662819 1103 62-63 -2.341693008336428 1103 64-65 -1.214711831742754 1103 66-67 -2.747338438237378 1103 68-69 -2.9911722075880114 1103 70-71 -4.556027519171664 1103 72-73 -4.206477914229055 1103 74-75 -0.6149325683030042 1103 76 -0.6551342461966563 1104 1 -1.2555262854258658 1104 2 0.20129775206869027 1104 3 0.2267375356343848 1104 4 -0.3792866194059812 1104 5 0.35916414992408363 1104 6 0.3592099343046442 1104 7 0.7240864719803923 1104 8 0.9633118956342841 1104 9 0.9111132505808399 1104 10-11 -0.3073102897523512 1104 12-13 -0.6568514263469112 1104 14-15 0.6539356960119349 1104 16-17 -0.7222302970077834 1104 18-19 -0.15303146783512034 1104 20-21 -0.4937524029751472 1104 22-23 -1.3499514763950842 1104 24-25 0.07614267092330351 1104 26-27 0.23723909434762902 1104 28-29 1.3623030214336183 1104 30-31 1.3438819728715856 1104 32-33 1.7548142080476623 1104 34-35 -0.4634913834762102 1104 36-37 0.8810772288901845 1104 38-39 1.4277171807968614 1104 40-41 3.4216530512129992 1104 42-43 0.5021828398142247 1104 44-45 1.7573461589353911 1104 46-47 1.2520638213831177 1104 48-49 0.45967570497776933 1104 50-51 -0.14400485802738672 1104 52-53 0.890288138877132 1104 54-55 0.7065003284130142 1104 56-57 1.2453299066480383 1104 58-59 -3.277562970372472 1104 60-61 -2.1003060867739336 1104 62-63 0.6062236583302365 1104 64-65 -0.41957294285386837 1104 66-67 -1.8515051049040423 1104 68-69 1.085216681300878 1104 70-71 -1.9449164080605534 1104 72-73 1.0574109746598346 1104 74-75 0.8572896539192172 1104 76 -2.7176342461966563 1105 1 0.9736403812407985 1105 2 0.30248822825916477 1105 3 0.7267375356343848 1105 4 -0.5370247146440761 1105 5 -1.4830977548378215 1105 6 0.3383766009713085 1105 7 0.8282531386470566 1105 8 -2.021807151984767 1105 9 0.20873229819989092 1105 10-11 0.011142091200028403 1105 12-13 -0.15833952158500608 1105 14-15 0.7208999817262196 1105 16-17 -0.4067541065315936 1105 18-19 -0.7185076583113101 1105 20-21 0.4065452160724732 1105 22-23 0.11433423789063113 1105 24-25 0.2160236233042525 1105 26-27 1.81610814196668 1105 28-29 0.5959339738145673 1105 30-31 1.526917687157301 1105 32-33 2.164040398523852 1105 34-35 -0.4411699549047796 1105 36-37 1.2084581812711335 1105 38-39 0.06313384746352568 1105 40-41 1.676117336927284 1105 42-43 1.4932542683856553 1105 44-45 0.9121080636972962 1105 46-47 1.7118852499545483 1105 48-49 -2.053717152165092 1105 50-51 1.1164118086392847 1105 52-53 1.5227286150676065 1105 54-55 1.5130479474606346 1105 56-57 1.1768775256956587 1105 58-59 0.6138060772465792 1105 60-61 2.139277246559395 1105 62-63 -0.1274072940507125 1105 64-65 1.2337008666699418 1105 66-67 0.0264710855721475 1105 68-69 -1.1499023663181696 1105 70-71 -1.4806306937748381 1105 72-73 -2.686636644387786 1105 74-75 -0.5950912984617354 1105 76 -3.7027532938157037 1106 1 -2.559692952092533 1106 2 -0.04036891459797687 1106 3 0.1267375356343834 1106 4 0.09154671392735025 1106 5 0.1549974832574179 1106 6 0.47170993430464137 1106 7 -0.7050801946862748 1106 8 0.7591452289676184 1106 9 -0.9055534160858301 1106 10-11 0.09685637691431737 1106 12-13 1.8368985736530874 1106 14-15 -1.9314809706547251 1106 16-17 0.43610303632554803 1106 18-19 1.205301865498214 1106 20-21 1.520830930358187 1106 22-23 1.2333818569382515 1106 24-25 -1.6696906624100336 1106 26-27 1.5018224276809633 1106 28-29 0.8435530214336211 1106 30-31 0.8126319728715856 1106 32-33 -0.39310245861900484 1106 34-35 -0.30307471680954734 1106 36-37 1.8894105622235173 1106 38-39 -3.4035328192031393 1106 40-41 1.8904030512129992 1106 42-43 0.5313495064808933 1106 44-45 0.8406794922687197 1106 46-47 1.016647154716452 1106 48-49 1.136759038311098 1106 50-51 -1.783588191360721 1106 52-53 1.3798714722104677 1106 54-55 -0.83933300492032 1106 56-57 0.6578299066480398 1106 58-59 0.9661870296275268 1106 60-61 2.5059439132260692 1106 62-63 1.924973658330238 1106 64-65 -0.5424896095205369 1106 66-67 0.569328228429292 1106 68-69 3.397716681300878 1106 70-71 -0.19491640806055344 1106 72-73 -3.634255692006832 1106 74-75 -2.8093770127474507 1106 76 -2.6551342461966563 1107 1 1.3069737145741342 1107 2 -1.2263338268786796 1107 3 0.04252700931859721 1107 4 -0.02073398782703606 1107 5 0.42166414992408363 1107 6 0.5664467764099044 1107 7 1.0563233140856525 1107 8 0.8679171587921815 1107 9 -0.049413065208632645 1107 10-11 0.7091370786687037 1107 12-13 0.9895301526004587 1107 14-15 0.882554117064565 1107 16-17 1.3238223345711617 1107 18-19 1.3667053742701398 1107 20-21 1.629602860182743 1107 22-23 -0.23153042376350896 1107 24-25 1.526800565660146 1107 26-27 -0.8630898530207958 1107 28-29 1.3803951266967829 1107 30-31 1.3126319728715856 1107 32-33 2.15777473436345 1107 34-35 0.4197323007343172 1107 36-37 2.2034456499428146 1107 38-39 2.7385724439547587 1107 40-41 2.2851398933182594 1107 42-43 1.6173144187615947 1107 44-45 1.1652408957774938 1107 46-47 1.6517348740146929 1107 48-49 1.0665835997146118 1107 50-51 1.660271457762093 1107 52-53 1.9149591915087072 1107 54-55 2.551895065255124 1107 56-57 2.320987801384881 1107 58-59 3.197765976995953 1107 60-61 2.963838650068176 1107 62-63 2.140763132014449 1107 64-65 2.687334951882974 1107 66-67 2.0728370003591152 1107 68-69 3.5029798391956177 1107 70-71 -0.7212321975342384 1107 72-73 0.5639899220282523 1107 74-75 0.725710706550796 1107 76 1.6080236485401862 1108 1 0.25434213562676433 1108 2 0.08945564680553275 1108 3 -0.6416835169971975 1108 4 0.29505548585717634 1108 5 -0.20991479744433406 1108 6 0.2506573027256991 1108 7 0.0563233140856525 1108 8 -0.7110302096288734 1108 9 -0.2073078020507424 1108 10-11 0.02492655235290897 1108 12-13 1.252688047337294 1108 14-15 0.9878172749593048 1108 16-17 0.9817170714132715 1108 18-19 0.3140737953227699 1108 20-21 1.2874975970248528 1108 22-23 0.005311681499655663 1108 24-25 1.3162742498706734 1108 26-27 0.24217330487394406 1108 28-29 -0.2511838206716419 1108 30-31 0.7336846044505307 1108 32-33 0.31566947120555966 1108 34-35 0.2618375638922146 1108 36-37 2.1771298604691296 1108 38-39 1.8438356018494915 1108 40-41 2.2851398933182594 1108 42-43 0.9594196819194849 1108 44-45 1.5073461589353911 1108 46-47 1.0201559266462752 1108 48-49 1.5928993891882897 1108 50-51 0.3181661946041885 1108 52-53 0.07285392835081694 1108 54-55 -0.5270523031659309 1108 56-57 1.3999351698059286 1108 58-59 -0.03907612826721163 1108 60-61 2.3585754921734363 1108 62-63 1.8512894478039215 1108 64-65 1.3715454781987617 1108 66-67 2.3096791056222727 1108 68-69 2.450348260248248 1108 70-71 1.8840309603605014 1108 72-73 -1.0939048148138504 1108 74-75 0.06781596970868975 1108 76 -1.4446079304071837 1109 1 -0.6930262854258658 1109 2 0.5762977520686903 1109 3 0.7267375356343848 1109 4 0.12071338059401882 1109 5 0.42166414992408363 1109 6 -1.1407900656953558 1109 7 0.03658647198039233 1109 8 -0.1616881043657159 1109 9 0.6611132505808399 1109 10-11 0.7864397102476488 1109 12-13 0.09314857365308882 1109 14-15 1.153935696011935 1109 16-17 0.8402697029922166 1109 18-19 0.7532185321648797 1109 20-21 -0.24375240297514722 1109 22-23 0.5562985236049158 1109 24-25 -3.7363573290766965 1109 26-27 -0.606510905652371 1109 28-29 -2.4814469785663817 1109 30-31 -0.8748680271284144 1109 32-33 1.6923142080476623 1109 34-35 0.3177586165237898 1109 36-37 2.3810772288901845 1109 38-39 2.0839671807968614 1109 40-41 -1.3595969487870008 1109 42-43 -0.43531716018577526 1109 44-45 1.4448461589353911 1109 46-47 0.5958138213831177 1109 48-49 -0.009074295022230672 1109 50-51 -0.3627548580273867 1109 52-53 -2.109711861122868 1109 54-55 0.36275032841301424 1109 56-57 -1.7546700933519617 1109 58-59 1.2849370296275282 1109 60-61 1.1809439132260664 1109 62-63 -0.8937763416697635 1109 64-65 1.5179270571461316 1109 66-67 -2.3515051049040423 1109 68-69 -3.039783318699122 1109 70-71 0.9300835919394466 1109 72-73 -2.6300890253401654 1109 74-75 -0.5802103460807828 1109 76 -2.0301342461966563 1110 1 -2.2812615795435143 1110 2 -0.9384081302842517 1110 3 0.13850224151673984 1110 4 0.2640957335351928 1110 5 0.42166414992408363 1110 6 0.2599452284222892 1110 7 0.45570411903921837 1110 8 0.849341307398987 1110 9 0.9846426623455429 1110 10-11 -0.07569264269352516 1110 12-13 1.3486632795354367 1110 14-15 1.3763621666001669 1110 16-17 0.9615932324039846 1110 18-19 0.47196853216487966 1110 20-21 2.052203479377795 1110 22-23 1.0176955824284448 1110 24-25 -1.2069455643708125 1110 26-27 2.092018506112339 1110 28-29 1.7317883155512703 1110 30-31 0.13616138463629568 1110 32-33 2.1500347962829522 1110 34-35 1.5126115577002608 1110 36-37 2.6972536994784164 1110 38-39 2.7199965925615643 1110 40-41 -0.22724400761052976 1110 42-43 0.5940946045201088 1110 44-45 1.5661696883471592 1110 46-47 1.2833138213831177 1110 48-49 2.5387198226248273 1110 50-51 1.1850392596196713 1110 52-53 1.723008727112422 1110 54-55 -0.37070555393992777 1110 56-57 1.2931240242950963 1110 58-59 0.21324585315693412 1110 60-61 -1.0764090279504046 1110 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0.26662205347790646 2116 72-73 -1.1060505638017055 2116 74-75 1.3572896539192172 2116 76 1.0371734461110371 2117 1 1.092688000288419 2117 2 0.8262977520686903 2117 3 0.7267375356343848 2117 4 -0.7275009051202659 2117 5 0.35023557849551423 2117 6 0.10028136287607481 2117 7 0.8758721862661076 2117 8 0.0972404670628535 2117 9 -0.7674581779905907 2117 10-11 -1.6198102897523512 2117 12-13 -1.8131014263469112 2117 14-15 -1.957671446845211 2117 16-17 -2.025801725579214 2117 18-19 -0.20660289640655094 2117 20-21 -0.10535954583228602 2117 22-23 -0.09995147639508417 2117 24-25 -4.914928757648127 2117 26-27 -2.5172251913666557 2117 28-29 -2.427875549994951 2117 30-31 -2.223082312842699 2117 32-33 -4.455007220523768 2117 34-35 0.13025861652378978 2117 36-37 -0.7082084853955308 2117 38-39 -3.4606756763459963 2117 40-41 -7.859596948787001 2117 42-43 -2.47103144590006 2117 44-45 -4.956939555350324 2117 46-47 -1.7166861786168823 2117 48-49 -1.3394314378793695 2117 50-51 -1.9073977151702408 2117 52-53 1.1536809960199932 2117 54-55 -1.963142528729847 2117 56-57 0.16497276379089953 2117 58-59 -2.183812970372472 2117 60-61 -3.5511989439167913 2117 62-63 -0.5678834845269023 2117 64-65 -1.8972515142824378 2117 66-67 -0.3782908191897505 2117 68-69 -0.0665690329848374 2117 70-71 -2.802059265203411 2117 72-73 -3.639017596768735 2117 74-75 -3.1784246317950675 2117 76 -1.8694199604823716 2118 1 -0.893026285425865 2118 2 0.6262977520686874 2118 3 -1.539929131032281 2118 4 -0.641786619405984 2118 5 0.35499748325741365 2118 6 0.20504326763797565 2118 7 0.8949198053137266 2118 8 0.6924785623009484 2118 9 -3.2388867494191587 2118 10-11 -1.1031436230856855 2118 12-13 -0.7964347596802455 2118 14-15 1.0018523626785978 2118 16-17 -4.9638969636744505 2118 18-19 -1.7613648011684546 2118 20-21 0.35416426369151566 2118 22-23 0.7333818569382444 2118 24-25 -0.13635732907670217 2118 26-27 -2.364844238985704 2118 28-29 -0.5231136452330531 2118 30-31 -1.95403469379508 2118 32-33 -2.126435791952332 2118 34-35 0.2969252831904612 2118 36-37 -4.843922771109817 2118 38-39 -3.70353281920314 2118 40-41 -2.6429302821203358 2118 42-43 -1.7686504935191039 2118 44-45 -2.6926538410646046 2118 46-47 -0.11668617861688801 2118 48-49 -0.2299076283555621 2118 50-51 -1.2502548580273896 2118 52-53 -3.6201285277895323 2118 54-55 0.4606669950796771 2118 56-57 0.32449657331470405 2118 58-59 -2.800479637039139 2118 60-61 -2.360722753440605 2118 62-63 -3.74169300833643 2118 64-65 -0.04248960952053693 2118 66-67 -1.1306717715707109 2118 68-69 -3.6689499853657885 2118 70-71 -3.094916408060552 2118 72-73 2.5990776413265024 2118 74-75 1.7906229872525508 2118 76 -0.5884675795299898 2119 1 0.034246441846860876 2119 2 -1.2646113388403997 2119 3 -1.2732624643656152 2119 4 -0.5326957103150747 2119 5 0.33075505901499014 2119 6 0.30807357066827734 2119 7 -0.7475044371105142 2119 8 -0.15600628618389578 2119 9 0.8883859778535665 2119 10-11 -0.41526483520689794 2119 12-13 1.073262210016729 2119 14-15 0.6624584232846615 2119 16-17 0.08458788481039647 2119 18-19 -0.3007587405623937 2119 20-21 -1.1670478575206005 2119 22-23 -0.5544969309405374 2119 24-25 -0.5545391472585166 2119 26-27 -0.049692723834183994 2119 28-29 1.4617348396154313 2119 30-31 2.3580865183261324 2119 32-33 1.837200571684022 2119 34-35 0.13025861652378978 2119 36-37 0.4833499561629111 2119 38-39 -2.558078273748592 2119 40-41 0.07222123303118622 2119 42-43 0.701046476177865 2119 44-45 0.8709825225717509 2119 46-47 1.237859275928571 2119 48-49 0.12160752315958945 2119 50-51 0.99519968742716 2119 52-53 0.9101745025134917 2119 54-55 0.5303639647766545 2119 56-57 -2.8058064569883214 2119 58-59 -0.2519947885542848 2119 60-61 1.2604893677715197 2119 62-63 0.6431554765120566 2119 64-65 -0.16673203376296186 2119 66-67 0.15417671327777782 2119 68-69 2.0340803176645146 2119 70-71 -1.3767345898787333 2119 72-73 0.9324109746598346 2119 74-75 0.5391078357373971 2119 76 3.253956662894254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 2.0 24 12.0 25 15.0 26 36.0 27 66.0 28 121.0 29 192.0 30 303.0 31 419.0 32 600.0 33 848.0 34 1167.0 35 1237.0 36 469.0 37 181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.25119068618804 16.087493385076733 10.919033339213264 68.74228258952196 2 1.7992591285941082 3.6690774387017107 82.76591991532898 11.7657435173752 3 2.363732580702064 17.040042335508907 70.71793967189981 9.878285411889221 4 1.7463397424589875 59.304992062092076 22.314341153642616 16.634327041806316 5 1.6934203563238666 27.65919915328982 66.66078673487388 3.986593755512436 6 2.8400070559181514 67.5780560945493 11.130710883753748 18.451225965778796 7 2.8929264420532723 28.258952196154524 9.490209913565002 59.3579114482272 8 2.5224907391074263 67.59569588992768 15.822896454401128 14.058916916563769 9 2.5401305344858 13.653201622861175 4.462868230728524 79.34379961192451 10-11 2.672428999823602 38.35773505027342 39.29264420532722 19.67719174457576 12-13 9.243252778267772 6.597283471511731 40.01587581584054 44.143587934379966 14-15 11.562885870523901 9.781266537308166 10.292820603281001 68.36302698888693 16-17 36.82307285235491 32.85411889222085 2.575410125242547 27.74739813018169 18-19 37.405186099841245 17.19880049391427 2.9899453166343273 42.40606808961016 20-21 13.000529193861352 6.121008996295643 2.8135473628505907 78.06491444699242 22-23 11.730463926618452 42.203210442758866 2.496031046039866 43.57029458458282 24-25 36.82307285235491 42.56482624801553 3.5544187687422824 17.05768213088728 26-27 37.572764155935786 46.798377138825195 9.55194919738931 6.076909507849709 28-29 6.191568177809137 53.20162286117481 5.785852884106545 34.82095607690951 30-31 9.155053801375903 42.106191568177806 41.15364261774563 7.585112012700653 32-33 32.077967895572414 48.36831892750044 16.26389133886047 3.289821838066678 34-35 10.372199682483684 16.722526018698183 69.81831010760276 3.086964191215382 36-37 2.628329511377668 63.44152407832069 23.505027341682837 10.425119068618805 38-39 8.837537484565178 16.325630622684777 35.47362850590933 39.36320338684071 40-41 5.362497795025577 10.442758863997177 45.0520373963662 39.142705944611045 42-43 40.35985182571882 5.318398306579644 43.270418063150466 11.051331804551069 44-45 8.237784441700477 34.01834538719351 40.82730640324572 16.916563767860293 46-47 40.712647733286296 6.3767860292820595 17.727994355265476 35.18257188216617 48-49 35.059093314517554 3.0340448050802613 13.115187863820779 48.79167401658141 50-51 9.948844593402717 3.2369024519315572 44.39054506967719 42.42370788498853 52-53 8.11430587405186 3.492679484917975 74.67807373434468 13.714940906685483 54-55 4.304110072323161 3.827835597107074 50.57329334979714 41.29476098077262 56-57 39.66307990827306 3.730816722526019 20.25930499206209 36.346798377138825 58-59 7.717410478038454 17.00476274475216 63.759040395131414 11.518786382077968 60-61 33.542070911977426 48.41241841594638 14.05009701887458 3.995413653201623 62-63 5.49479626036338 87.59040395131417 3.0164050097018875 3.898394778620568 64-65 1.5523019932968778 90.86258599400246 2.6371494090668546 4.947962603633798 66-67 2.169694831539954 89.39848297759745 2.848826953607338 5.582995237255248 68-69 2.443111659904745 84.66219791850415 3.4485799964720414 9.446110425119068 70-71 3.1046039865937556 76.31857470453343 6.297406950079379 14.279414358793439 72-73 4.260010583877227 61.23654965602399 10.416299170929618 24.087140589169167 74-75 5.097900864349973 58.84635738225437 10.619156817780913 25.436584935614746 76 3.6514376433233373 61.210089962956424 10.336920091726936 24.801552301993297 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 3.0 25 4.0 26 3.0 27 2.0 28 2.0 29 2.0 30 2.0 31 10.0 32 15.5 33 13.0 34 23.0 35 92.0 36 470.5 37 790.0 38 1123.5 39 1341.0 40 1021.5 41 628.0 42 438.0 43 317.0 44 159.0 45 112.0 46 102.0 47 74.0 48 36.5 49 27.5 50 28.0 51 25.0 52 19.0 53 14.0 54 12.0 55 17.5 56 24.0 57 19.5 58 14.0 59 15.5 60 17.0 61 15.0 62 13.0 63 10.0 64 6.5 65 5.5 66 6.5 67 8.0 68 4.5 69 1.5 70 1.5 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 5669.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.444522843535015 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04918032786885 15.752337272887635 2 11.04400345125108 4.515787616863644 3 3.710094909404659 2.275533603810196 4 1.8981880931837791 1.5523019932968778 5 1.1216566005176878 1.1465866995942848 6 0.5176876617773942 0.63503263362145 7 0.6039689387402933 0.8643499735403068 8 0.6039689387402933 0.9878285411889223 9 0.3451251078515962 0.63503263362145 >10 2.5021570319240722 9.596048685835244 >50 0.08628127696289906 0.9878285411889223 >100 0.3451251078515962 12.524254718645263 >500 0.08628127696289906 9.419650732051508 >1k 0.08628127696289906 39.107426353854294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 2217 39.107426353854294 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 534 9.419650732051508 RNA PCR Primer, Index 15 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 273 4.815664138295996 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 230 4.05715293702593 No Hit CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 105 1.8521785147292291 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 102 1.7992591285941082 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 56 0.9878285411889223 No Hit CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 50 0.8819897689186805 No Hit TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT 49 0.8643499735403068 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT 46 0.8114305874051861 No Hit CTTATACACATCTCCGAGCCCACGAGCCATAAGTTAATCTCGTATGCCGT 25 0.44099488445934026 No Hit CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT 22 0.38807549832421945 No Hit CTTATACACATCTCCGAGCCCACGCGACATAAGTTAATCTCGTATGCCGT 22 0.38807549832421945 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCG 20 0.35279590756747226 No Hit ACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCT 19 0.3351561121890986 RNA PCR Primer, Index 15 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT 19 0.3351561121890986 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCC 19 0.3351561121890986 No Hit CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT 18 0.317516316810725 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGTCTT 17 0.2998765214323514 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 17 0.2998765214323514 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCG 17 0.2998765214323514 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTAGTATGCCGT 17 0.2998765214323514 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCC 16 0.28223672605397776 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT 15 0.2645969306756042 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT 14 0.24695713529723057 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGTCTT 13 0.22931733991885692 No Hit TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 13 0.22931733991885692 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTCATCTCGTATGCCGT 12 0.21167754454048332 No Hit CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 11 0.19403774916210972 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT 11 0.19403774916210972 No Hit CTTATACACATCTACGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 11 0.19403774916210972 No Hit ATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTGCTT 11 0.19403774916210972 Illumina PCR Primer Index 8 (96% over 26bp) CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 10 0.17639795378373613 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG 10 0.17639795378373613 No Hit CTTATACACATCTCCGAGCCCAAGAGACATAAGTTAATCTCGTATGCCGT 10 0.17639795378373613 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTACTCTCGTATGCCGT 10 0.17639795378373613 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCC 9 0.1587581584053625 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG 9 0.1587581584053625 No Hit CTTATAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 9 0.1587581584053625 No Hit CTTATACACATCTCCGAGCACACGAGACATAAGTTAATCTCGTATGCCGT 9 0.1587581584053625 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGTCTT 8 0.14111836302698888 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGC 8 0.14111836302698888 No Hit AATGTGGTTAGTTACCACACCCTCTCTGCCTGACTTTTCATTCCTGCCTC 8 0.14111836302698888 No Hit ATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGTCTT 8 0.14111836302698888 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTCTGCCGT 8 0.14111836302698888 No Hit TCTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 8 0.14111836302698888 No Hit CTTATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT 8 0.14111836302698888 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCACGTATGCCGT 7 0.12347856764861528 No Hit ATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGTCTT 7 0.12347856764861528 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCCCGTATGCCGT 7 0.12347856764861528 No Hit CTTATACACATCTCCGAGCCAACGAGACATAAGTTAATCTCGTATGCCGT 7 0.12347856764861528 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAAATCGTATGCCGT 7 0.12347856764861528 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTCTGCCGTCTT 7 0.12347856764861528 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 7 0.12347856764861528 No Hit CATTATAATAAGGGCTTTTGTGTCAGAAACATATAAAATCAAAACCCTTA 6 0.10583877227024166 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCG 6 0.10583877227024166 No Hit CTTATACACATATCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 6 0.10583877227024166 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATTTCGTATGCCGT 6 0.10583877227024166 No Hit GGGCTGAATGTCCACGTTCTGCCTGTGGACAGGGCCTCGGGTGAGAGCTG 6 0.10583877227024166 No Hit CTTATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 6 0.10583877227024166 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.14111836302698888 0.0 2 0.0 0.0 0.0 0.14111836302698888 0.0 3 0.0 0.0 0.0 0.14111836302698888 0.0 4 0.0 0.0 0.0 0.14111836302698888 0.0 5 0.0 0.0 0.0 0.1587581584053625 0.0 6 0.0 0.0 0.0 0.1587581584053625 0.0 7 0.0 0.0 0.0 0.1587581584053625 0.0 8 0.0 0.0 0.0 0.1587581584053625 0.0 9 0.0 0.0 0.0 0.1587581584053625 0.0 10 0.0 0.0 0.0 0.1587581584053625 0.0 11 0.0 0.0 0.0 0.1587581584053625 0.0 12 0.0 0.0 0.0 0.22931733991885694 0.0 13 0.0 0.0 0.0 0.28223672605397776 0.0 14 0.0 0.0 0.0 0.317516316810725 0.0 15 0.0 0.0 0.0 0.40571529370259307 0.0 16 0.0 0.0 0.0 0.42335508908096664 0.0 17 0.0 0.0 0.0 0.4586346798377139 0.0 18 0.0 0.0 0.0 0.4762744752160875 0.0 19 0.0 0.0 0.0 0.7408714058916916 0.0 20 0.0 0.0 0.0 0.8290703827835597 0.0 21 0.0 0.0 0.0 0.8467101781619333 0.0 22 0.0 0.0 0.0 0.8467101781619333 0.0 23 0.0 0.0 0.0 0.8819897689186805 0.0 24 0.0 0.0 0.0 0.8819897689186805 0.0 25 0.0 0.0 0.0 0.8996295642970542 0.0 26 0.0 0.0 0.0 0.8996295642970542 0.0 27 0.0 0.0 0.0 0.9172693596754278 0.0 28 0.0 0.0 0.0 0.9172693596754278 0.0 29 0.0 0.0 0.0 0.9172693596754278 0.0 30 0.0 0.0 0.0 0.952548950432175 0.0 31 0.0 0.0 0.0 1.0760275180807903 0.0 32 0.0 0.0 0.0 1.128946904215911 0.0 33 0.0 0.0 0.0 1.217145881107779 0.0 34 0.0 0.0 0.0 1.2524254718645265 0.0 35 0.0 0.0 0.0 1.3229846533780207 0.0 36 0.0 0.0 0.0 1.358264244134768 0.0 37 0.0 0.0 0.0 1.6228611748103723 0.0 38 0.0 0.0 0.0 1.7463397424589875 0.0 39 0.0 0.0 0.0 2.046216263891339 0.0 40 0.0 0.0 0.0 2.187334626918328 0.0 41 0.0 0.0 0.0 2.434291762215558 0.0 42 0.0 0.0 0.0 2.451931557593932 0.0 43 0.0 0.0 0.0 2.504850943729053 0.0 44 0.0 0.0 0.0 2.7518080790262833 0.0 45 0.0 0.0 0.0 3.1046039865937556 0.0 46 0.0 0.0 0.0 3.2810019403774917 0.0 47 0.0 0.0 0.0 3.439760098782854 0.0 48 0.0 0.0 0.0 3.598518257188217 0.0 49 0.0 0.0 0.0 3.7749162109719525 0.0 50 0.0 0.0 0.0 3.9160345739989415 0.0 51 0.0 0.0 0.0 4.00423355089081 0.0 52 0.0 0.0 0.0 4.233550890809666 0.0 53 0.0 0.0 0.0 4.321749867701534 0.0 54 0.0 0.0 0.0 4.44522843535015 0.0 55 0.0 0.0 0.0 4.621626389133886 0.0 56 0.0 0.0 0.0 4.6745457752690065 0.0 57 0.0 0.0 0.0 4.69218557064738 0.0 58 0.0 0.0 0.0 4.745104956782502 0.0 59 0.0 0.0 0.0 4.921502910566238 0.0 60 0.0 0.0 0.0 5.009701887458106 0.0 61 0.0 0.0 0.0 5.062621273593226 0.0 62 0.0 0.0 0.0 5.168460045863468 0.0 63 0.0 0.0 0.0 5.27429881813371 0.0 64 0.0 0.0 0.0 5.3272182042688305 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACAA 15 0.0021117597 70.00001 70 CTTTACA 25 2.4919646E-6 70.0 1 TTTACAC 25 2.4919646E-6 70.0 2 TTACACA 35 1.833356E-5 50.0 3 AACAACA 30 5.394139E-4 46.666668 69 CTTATAC 365 0.0 46.027397 1 TATACAC 380 0.0 44.21053 3 TTATACA 380 0.0 44.21053 2 CTCTTAT 75 2.5975169E-8 37.333332 1 ACTCGTA 40 0.0022220216 35.0 38 AAACTCG 40 0.0022220216 35.0 36 TAAACTC 40 0.0022220216 35.0 35 AAACAAC 50 1.5051136E-4 35.0 68 AACTCGT 40 0.0022220216 35.0 37 TTAAACT 40 0.0022220216 35.0 34 GTTAAAC 40 0.0022220216 35.0 33 ATCTCGT 410 0.0 34.146343 37 AATCTCG 400 0.0 34.125 36 TAATCTC 400 0.0 34.125 35 GAAAAAA 475 0.0 33.894737 60 >>END_MODULE