FastQCFastQC Report
Wed 25 May 2016
SRR1780679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46544
Sequences flagged as poor quality0
Sequence length50
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATC710.1525438294946717No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC670.14394981093159162No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTGACGCTGCCGACG610.13105878308697147No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG600.12891027844620145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAG207.755239E-444.0269112
CTTCTAG207.796525E-443.9795729
ACGAACT207.796525E-443.9795724
TTACACA404.0266787E-738.4821243
CTTTACA301.285497E-436.6496431
TTCTAGG250.002334992935.18365530
CGAACTT250.002334992935.18365525
ACTTCTA250.002334992935.18365528
TTTACAC401.7885854E-532.9846762
CTATACA353.1795335E-431.4139791
GACGAAC353.1795335E-431.41397923
GGTGGTC502.306002E-630.78569844
GAAGCTG300.005672076329.35127415
TGAAGCT300.005672076329.35127414
TGACGCC300.005672076329.35127413
CTGAAGC300.005672076329.35127413
GGCTCTT300.005672076329.35127439
GAACTTC300.00570204129.31971526
AACTTCT300.00570204129.31971527
CGGTGGT602.855395E-729.31971543
Produced by FastQC (version 0.11.5)