FastQCFastQC Report
Wed 25 May 2016
SRR1780679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46544
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2559954.99957029907184No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT46069.896012375386732Illumina PCR Primer Index 11 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG37017.951615675489859No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC32286.935372980405638No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC6621.422310072189756No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT4540.975421106909591Illumina PCR Primer Index 11 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3970.8529563423856995No Hit
ACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCT2360.5070470952217256Illumina PCR Primer Index 11 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2110.45333447920247505No Hit
ATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCTT2060.44259195599862494Illumina PCR Primer Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCT1860.39962186318322446Illumina PCR Primer Index 11 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1020.21914747335854248No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG960.2062564455139223No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATATCGTATGCCGT960.2062564455139223No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTA910.19551392231007217No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC720.15469233413544173No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG670.14394981093159162No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG630.13535579236851153No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG580.1246132691646614No Hit
TCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCTTG540.1160192506015813Illumina PCR Primer Index 11 (96% over 29bp)
CTTATTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG530.11387074596081127No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG530.11387074596081127No Hit
CTTATCACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC510.10957373667927124No Hit
CTTAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC490.10527672739773118No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG470.10097971811619112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT408.019924E-944.01
CCGTCTT5300.038.6037744
CTTTACA651.0913936E-1137.230771
TTTACAC651.0913936E-1137.230772
TTTATAC553.276E-936.03
CTTTATA505.7389116E-835.22
CTGCTTG250.002329678335.244
ATGCCGT33550.034.68852644
GCCGTCT5950.034.38655543
CTCTTAT5250.033.9428561
CTTATAC35250.032.7035451
TTATACA35650.032.3366052
TTACACA755.820766E-1132.2666663
CTATACA551.326116E-732.01
TATACAC36650.031.5743543
TATGCCG37700.030.8700343
TATCGTA300.005689115329.33333238
CTCCATA300.005689115329.33333233
TCTGCTT300.005689115329.33333243
TCCATAT300.005689115329.33333234
Produced by FastQC (version 0.11.5)