FastQCFastQC Report
Wed 25 May 2016
SRR1780678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23678
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT320.13514654953965705No Hit
GGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG290.12247656052031422No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA150.002199828669.985246
TTACCAT150.002199828669.985254
ACGCATC150.002199828669.985213
ATTGGCC150.002199828669.985268
TACGCAT150.002199828669.985212
ACGATCC150.002199828669.985242
AAAGTCC150.002199828669.985229
GCATCCT150.002199828669.985215
TCCCTAC150.002199828669.98528
CGATCCC150.002199828669.985243
TTATAAT252.3457606E-455.9881619
GGTAGGT252.3457606E-455.9881649
TGGGTAG252.3457606E-455.9881647
GTTATAA252.3457606E-455.9881618
ACTATGT252.3457606E-455.9881629
GATGGGT200.0068743152.488945
CTTTTAA200.0068743152.488937
TGTTGGG200.0068743152.488961
TCCCATC200.0068743152.48893
CCTACGT200.0068743152.488934
Produced by FastQC (version 0.11.5)