##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780678_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23678 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.849733930230595 34.0 24.0 34.0 12.0 34.0 2 30.093039952698707 34.0 31.0 34.0 12.0 34.0 3 30.14828110482304 34.0 31.0 34.0 11.0 34.0 4 30.730340400371652 34.0 32.0 34.0 21.0 34.0 5 30.48251541515331 34.0 32.0 34.0 11.0 34.0 6 33.06913590674888 37.0 34.0 38.0 21.0 38.0 7 32.76661880226371 37.0 32.0 38.0 11.0 38.0 8 31.993158205929554 37.0 31.0 38.0 11.0 38.0 9 32.40641101444379 37.0 31.0 38.0 12.0 38.0 10-11 32.74427738829293 37.0 32.5 38.0 11.0 38.0 12-13 33.034208970352225 37.0 33.5 38.0 11.0 38.0 14-15 32.68945856913591 37.0 32.0 38.0 11.0 38.0 16-17 32.472463890531294 37.0 31.0 38.0 11.5 38.0 18-19 32.454007939859785 37.0 31.5 38.0 11.0 38.0 20-21 32.512923388799734 37.0 31.0 38.0 11.0 38.0 22-23 32.44452656474364 37.0 31.5 38.0 11.0 38.0 24-25 32.83799307373934 37.0 33.0 38.0 11.0 38.0 26-27 32.845193850832 37.0 33.5 38.0 11.0 38.0 28-29 32.904468282794156 37.0 34.0 38.0 11.0 38.0 30-31 32.80530450206943 37.0 34.0 38.0 11.0 38.0 32-33 32.630902103218176 37.0 32.5 38.0 11.0 38.0 34-35 32.552263704704785 37.0 31.5 38.0 11.0 38.0 36-37 32.52890869161247 37.0 31.5 38.0 11.0 38.0 38-39 32.91922882000169 37.0 34.0 38.0 11.0 38.0 40-41 32.4827054649886 37.0 31.5 38.0 11.0 38.0 42-43 32.803889686628935 37.0 33.5 38.0 11.0 38.0 44-45 31.889433229157866 37.0 29.0 38.0 11.0 38.0 46-47 32.55380522003547 37.0 31.5 38.0 11.0 38.0 48-49 32.35566770842132 37.0 31.0 38.0 11.0 38.0 50-51 32.22827096883183 37.0 31.0 38.0 11.0 38.0 52-53 32.344919334403244 37.0 31.0 38.0 11.0 38.0 54-55 32.08560689247403 37.0 31.0 38.0 11.0 38.0 56-57 32.08624039192499 37.0 31.0 38.0 11.0 38.0 58-59 31.990919841202803 37.0 30.0 38.0 11.0 38.0 60-61 31.966192245966724 37.0 31.0 38.0 11.0 38.0 62-63 32.07025508911226 37.0 31.0 38.0 11.0 38.0 64-65 32.0771602331278 37.0 31.0 38.0 11.0 38.0 66-67 32.096777599459415 37.0 31.0 38.0 11.0 38.0 68-69 31.667919587803024 37.0 29.0 38.0 11.0 38.0 70-71 31.49330602246811 37.0 27.0 38.0 11.0 38.0 72-73 31.573929385927865 37.0 28.0 38.0 11.0 38.0 74-75 31.26940619984796 36.5 27.0 38.0 11.0 38.0 76 30.63852521327815 36.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.0039230457429156 1101 2 -0.26824614416879555 1101 3 1.301597736734589 1101 4 0.28835351342645765 1101 5 0.8221054284051235 1101 6 -1.0551951532523134 1101 7 -1.637825526481496 1101 8 0.37196083484390385 1101 9 -0.6190216389659255 1101 10-11 0.26536250708386433 1101 12-13 0.23867087521854558 1101 14-15 1.9865119557165798 1101 16-17 -1.1871872759843356 1101 18-19 0.5498753850363514 1101 20-21 0.6172124401671084 1101 22-23 0.7320971669394751 1101 24-25 0.7737328509595969 1101 26-27 0.8877510156760948 1101 28-29 0.7843629151665183 1101 30-31 0.2004184061305878 1101 32-33 1.5233255620345574 1101 34-35 0.6267208331616132 1101 36-37 0.2955550277929788 1101 38-39 0.7488671054741971 1101 40-41 2.008639406979654 1101 42-43 1.347969143689717 1101 44-45 1.251994723033139 1101 46-47 1.8308494264767248 1101 48-49 -0.25506337326045525 1101 50-51 0.45976204382119334 1101 52-53 0.8381656266926711 1101 54-55 -0.7036322368757553 1101 56-57 0.8320651401726948 1101 58-59 0.301620874922925 1101 60-61 0.8306926758311377 1101 62-63 -0.5712312508768704 1101 64-65 0.818014454559794 1101 66-67 -0.044383322708576145 1101 68-69 1.2761379620391402 1101 70-71 1.0833023732384568 1101 72-73 1.5579811161045356 1101 74-75 -0.7276828245140727 1101 76 -0.2937102138469392 1102 1 0.933842588744028 1102 2 -0.6410451727055282 1102 3 0.4552103657570541 1102 4 0.3575702711678055 1102 5 0.4295735522666888 1102 6 1.0331472875491485 1102 7 1.2881003994444313 1102 8 2.3094228870600517 1102 9 0.633255228065039 1102 10-11 -0.6384019130739986 1102 12-13 -4.366216799341263 1102 14-15 -0.2026197989889411 1102 16-17 -1.58351393050771 1102 18-19 -0.9759291079812584 1102 20-21 -0.3585009781692605 1102 22-23 -0.11702244447521792 1102 24-25 0.4883655164119318 1102 26-27 -3.6219636169893548 1102 28-29 -1.136402112155892 1102 30-31 0.00627754395694069 1102 32-33 -6.97546010888226 1102 34-35 -1.915780684749734 1102 36-37 0.43732794825442767 1102 38-39 0.5832629767553144 1102 40-41 0.787024349299017 1102 42-43 0.39942633858954224 1102 44-45 -3.744362289717319 1102 46-47 0.19013904396306458 1102 48-49 -0.7784392081116991 1102 50-51 -6.380401890605036 1102 52-53 -1.6926479737930613 1102 54-55 0.6609700703494994 1102 56-57 -2.0870301846603354 1102 58-59 0.7223859022453354 1102 60-61 -0.20725510801828761 1102 62-63 -4.0826155860317 1102 64-65 -2.392975223642999 1102 66-67 -1.276623759867043 1102 68-69 -7.033212371901357 1102 70-71 -1.8860358174112086 1102 72-73 -1.002735338054535 1102 74-75 -6.367027086809156 1102 76 -4.887820717793506 1103 1 0.6442251024052297 1103 2 -0.3471511361172652 1103 3 0.8845284935783901 1103 4 0.9308655907211438 1103 5 0.1965015636708216 1103 6 -1.7677555397257407 1103 7 -0.07421844113527953 1103 8 0.6489334596426133 1103 9 1.5814614528215145 1103 10-11 1.0222063074059236 1103 12-13 0.041005818857833276 1103 14-15 -0.00946227938808164 1103 16-17 0.09823945509457133 1103 18-19 -0.32613105618748506 1103 20-21 0.4779209747886881 1103 22-23 1.1048829962470421 1103 24-25 0.33250901843141634 1103 26-27 -0.22174978947688118 1103 28-29 1.3785658137172376 1103 30-31 0.5800480357602211 1103 32-33 -3.731102779350305 1103 34-35 -0.6566930154696209 1103 36-37 -0.19880890135355145 1103 38-39 1.2218944806754877 1103 40-41 0.8186233039200701 1103 42-43 0.94337977170904 1103 44-45 -4.004043924309858 1103 46-47 -0.06407810975515105 1103 48-49 0.5629720534706237 1103 50-51 0.6372982757052483 1103 52-53 0.323270296579949 1103 54-55 0.6144031898553166 1103 56-57 0.2575884896090912 1103 58-59 0.43205565753162745 1103 60-61 0.5569406629486906 1103 62-63 0.9299764785917262 1103 64-65 0.7640692049623681 1103 66-67 -0.25452825024480674 1103 68-69 -2.5749087368336454 1103 70-71 0.6010157387779067 1103 72-73 1.533021373753492 1103 74-75 -1.2059436940792914 1103 76 0.8520224753640129 1104 1 -1.5415672473215452 1104 2 -0.21481566450880507 1104 3 0.7502923329701687 1104 4 0.09527518920059208 1104 5 1.1344915850535742 1104 6 0.22986859902455592 1104 7 1.6159692519034508 1104 8 1.4241769854207078 1104 9 0.5512880149502877 1104 10-11 1.0911062836473064 1104 12-13 0.28132418426529426 1104 14-15 1.5842654469126956 1104 16-17 1.0394368891644206 1104 18-19 0.4339069575925123 1104 20-21 1.6824826283881151 1104 22-23 1.1206824735575722 1104 24-25 0.9309884672316073 1104 26-27 1.4682003174368745 1104 28-29 0.8308110025982032 1104 30-31 0.6292283636290747 1104 32-33 0.05732677636364514 1104 34-35 1.6415963644305904 1104 36-37 0.33077057120524245 1104 38-39 0.41932855052581175 1104 40-41 1.7542374640531193 1104 42-43 0.7518853549829814 1104 44-45 -2.1951819618484656 1104 46-47 0.9196472406843768 1104 48-49 1.1477903000850276 1104 50-51 2.135991552017913 1104 52-53 -0.7664184655963382 1104 54-55 0.8003143326445823 1104 56-57 1.380182930093767 1104 58-59 1.1895990169994306 1104 60-61 1.087826859194827 1104 62-63 1.3600073647879753 1104 64-65 1.4348936288160203 1104 66-67 1.0512450925919694 1104 68-69 -2.525015650589882 1104 70-71 -0.17292106331284884 1104 72-73 0.9071007275192358 1104 74-75 -0.4244041359894837 1104 76 1.3744743641737038 1105 1 -1.0797332309281025 1105 2 -1.5437091071317575 1105 3 -0.82629578178393 1105 4 -0.8227575976846531 1105 5 -0.1587973493726551 1105 6 0.48473540230324375 1105 7 0.16310039944443133 1105 8 1.8158265755846443 1105 9 1.0146589165896316 1105 10-11 1.0359064885653453 1105 12-13 -2.0804842173740496 1105 14-15 -0.9116361924315655 1105 16-17 -0.03932847968803799 1105 18-19 0.08170408874005375 1105 20-21 -1.0818616339069678 1105 22-23 -0.5780880182457082 1105 24-25 0.08941572132996356 1105 26-27 -1.7951193546942719 1105 28-29 -1.960428751500153 1105 30-31 -0.8965144642397789 1105 32-33 -0.05537814166914501 1105 34-35 0.5922879627912465 1105 36-37 0.044397620385574044 1105 38-39 -0.8304153019332077 1105 40-41 -0.7373096670944221 1105 42-43 -0.9792878007547259 1105 44-45 0.9377586119220247 1105 46-47 -0.049358906856603824 1105 48-49 0.08029542303584236 1105 50-51 -0.5793004561788067 1105 52-53 -0.4537903918258479 1105 54-55 -1.1078567739127934 1105 56-57 -1.6537566190865647 1105 58-59 -0.1020249584104036 1105 60-61 -0.014504083428121817 1105 62-63 0.025990971345351 1105 64-65 -0.8900295269216869 1105 66-67 -0.28917498937524044 1105 68-69 0.07590648055765925 1105 70-71 0.08822135471993775 1105 72-73 -1.725583698710274 1105 74-75 -2.0245578245140727 1105 76 -2.475423176809901 1106 1 -1.8057748975947696 1106 2 0.4021242262015754 1106 3 1.0164125515494042 1106 4 0.9439090689820127 1106 5 1.6276609839606806 1106 6 1.6503604023032423 1106 7 2.4714337327777613 1106 8 1.69603490891798 1106 9 2.6365339165896344 1106 10-11 1.7385106552320053 1106 12-13 0.4710782826259532 1106 14-15 0.8216971409017688 1106 16-17 1.3091090203119649 1106 18-19 1.111912422073388 1106 20-21 0.8681383660930351 1106 22-23 1.3052453150876246 1106 24-25 1.435769887996635 1106 26-27 0.7840473119723939 1106 28-29 1.117696248499847 1106 30-31 1.1217147024268925 1106 32-33 1.2112885249975207 1106 34-35 1.173017129457918 1106 36-37 1.5964809537189097 1106 38-39 0.9247930314001209 1106 40-41 1.051231999572238 1106 42-43 1.0201913659119413 1106 44-45 1.7992169452553561 1106 46-47 1.2975160931433933 1106 48-49 1.941232923035848 1106 50-51 1.0847620438211933 1106 52-53 1.0529804415074864 1106 54-55 0.02599739275387236 1106 56-57 0.9228058809134367 1106 58-59 1.2766208749229335 1106 60-61 1.6547667499052068 1106 62-63 0.6259909713453524 1106 64-65 1.4948663064116445 1106 66-67 1.3806166772914281 1106 68-69 3.2779898138909935 1106 70-71 1.1231171880532749 1106 72-73 0.9764996346230603 1106 74-75 0.7723171754859273 1106 76 1.809993489856769 1107 1 -0.8176796594995324 1107 2 0.3402194642968155 1107 3 0.01165064678749772 1107 4 0.5629566880296331 1107 5 0.3443276506273456 1107 6 -1.0853538834110417 1107 7 0.08810039944442849 1107 8 -0.11586985298678343 1107 9 0.6793910594467718 1107 10-11 -0.3031560114346519 1107 12-13 0.46750685405451975 1107 14-15 0.2740780932827178 1107 16-17 -0.32660526540232127 1107 18-19 0.6678648030257648 1107 20-21 0.9002812232358934 1107 22-23 -1.1388023039599915 1107 24-25 -0.06303963581289196 1107 26-27 0.024523502448580814 1107 28-29 -0.3394466086430086 1107 30-31 -0.8842376785254942 1107 32-33 0.6410504297594244 1107 34-35 -0.47222096578018125 1107 36-37 0.7452904775284281 1107 38-39 1.5343168409239354 1107 40-41 0.5417081900484391 1107 42-43 0.4178104135309937 1107 44-45 -1.77340210236369 1107 46-47 0.06894466457196557 1107 48-49 0.7269472087501256 1107 50-51 0.6526191866783364 1107 52-53 1.655361393888441 1107 54-55 0.2664735832300593 1107 56-57 -0.14147983337227998 1107 58-59 1.0254303987324533 1107 60-61 -0.8428522977138364 1107 62-63 -1.2990090286546483 1107 64-65 0.5722472587926006 1107 66-67 1.110378582053329 1107 68-69 0.937513623414798 1107 70-71 -0.2566447167086352 1107 72-73 -0.28540512728169887 1107 74-75 -0.6848256816569283 1107 76 -0.9138160339527595 1108 1 0.15464176907189753 1108 2 0.5344158928682425 1108 3 -1.10754578178393 1108 4 -0.35400759768465306 1108 5 -0.3462973493726551 1108 6 0.7191104023032437 1108 7 1.1631003994444313 1108 8 -0.12167342441535567 1108 9 -1.0634660834103684 1108 10-11 0.4109064885653453 1108 12-13 1.0757657826259504 1108 14-15 0.9711763075684345 1108 16-17 -0.172140979688038 1108 18-19 0.38639158874005375 1108 20-21 1.1915758660930322 1108 22-23 -0.4062130182457082 1108 24-25 -0.9027717786700364 1108 26-27 -0.07636935469427186 1108 28-29 0.547383748499847 1108 30-31 0.4394230357602211 1108 32-33 0.483684358330855 1108 34-35 1.5376004627912465 1108 36-37 0.536585120385574 1108 38-39 0.7008346980667923 1108 40-41 0.004877832905577861 1108 42-43 0.4660246992452741 1108 44-45 -0.015366388077975301 1108 46-47 0.4428285931433962 1108 48-49 0.17404542303584236 1108 50-51 0.6394495438211933 1108 52-53 0.5618346081741521 1108 54-55 0.7437057260872066 1108 56-57 0.7290558809134353 1108 58-59 -0.8598374584104036 1108 60-61 0.9698709165718782 1108 62-63 0.518178471345351 1108 64-65 0.8834079730783131 1108 66-67 1.0467625106247596 1108 68-69 0.9430939805576593 1108 70-71 0.6194713547199378 1108 72-73 0.899416301289726 1108 74-75 1.1238796754859273 1108 76 0.7745768231900989 1109 1 0.5452667690718975 1109 2 -0.29370910713175746 1109 3 1.00182921821607 1109 4 0.20849240231534694 1109 5 0.9662026506273449 1109 6 -0.015264597696756255 1109 7 0.8506003994444313 1109 8 1.7845765755846443 1109 9 0.9677839165896316 1109 10-11 2.3796564885653453 1109 12-13 1.2945157826259504 1109 14-15 1.1352388075684345 1109 16-17 -0.172140979688038 1109 18-19 0.5035790887400537 1109 20-21 2.0822008660930322 1109 22-23 1.7344119817542918 1109 24-25 -0.05120927867003644 1109 26-27 0.18925564530572814 1109 28-29 0.961446248499847 1109 30-31 0.5097355357602211 1109 32-33 1.132121858330855 1109 34-35 0.6469754627912465 1109 36-37 1.013147620385574 1109 38-39 0.7164596980667923 1109 40-41 1.5673778329055779 1109 42-43 1.3800871992452741 1109 44-45 0.3518211119220247 1109 46-47 -0.8696714068566038 1109 48-49 -0.5603295769641576 1109 50-51 0.42851204382119334 1109 52-53 0.6477721081741521 1109 54-55 0.5249557260872066 1109 56-57 0.6196808809134353 1109 58-59 0.5386000415895964 1109 60-61 1.7589334165718782 1109 62-63 -0.317759028654649 1109 64-65 0.4849704730783131 1109 66-67 1.0389500106247596 1109 68-69 1.5759064805576593 1109 70-71 1.7210338547199378 1109 72-73 0.625978801289726 1109 74-75 2.2957546754859273 1109 76 1.712076823190099 1110 1 1.1317872914599576 1110 2 0.5896864152563026 1110 3 0.02188518836532438 1110 4 -0.15111580663987567 1110 5 -0.4556723493726551 1110 6 0.16453950678085505 1110 7 0.7060108472056257 1110 8 1.7097165009577822 1110 9 -1.891824292365591 1110 10-11 -1.3427082502406265 1110 12-13 0.21697380501400687 1110 14-15 -0.38551678944649126 1110 16-17 -0.23044321849400973 1110 18-19 0.13417610366542476 1110 20-21 -0.41954820107114443 1110 22-23 -0.176968615260634 1110 24-25 -0.40813558464018485 1110 26-27 -1.2288880114106888 1110 28-29 0.21471117387297767 1110 30-31 -0.08413106871739018 1110 32-33 0.9614129031069751 1110 34-35 0.5250071792091546 1110 36-37 0.26874463531094506 1110 38-39 1.2631014891115697 1110 40-41 0.94949070603991 1110 42-43 0.7729276843199031 1110 44-45 1.312525402966802 1110 46-47 0.9564713170239898 1110 48-49 1.2203374006477858 1110 50-51 1.5530456259107481 1110 52-53 0.8681545708607246 1110 54-55 -0.26305733361428807 1110 56-57 0.3899700600179159 1110 58-59 -3.188428876320849 1110 60-61 0.20576177478083224 1110 62-63 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0.3315518700789113 2116 54-55 -0.5819391151826352 2116 56-57 -0.3835433254357703 2116 58-59 -0.25393468063262503 2116 60-61 -0.19047134533288101 2116 62-63 0.5994036697580469 2116 64-65 0.3682790048243447 2116 66-67 -0.42533570366095574 2116 68-69 0.9295771154782919 2116 70-71 -1.6336288436927582 2116 72-73 1.2193567774802077 2116 74-75 -0.24355584038708855 2116 76 -0.8836573037940276 2117 1 -0.9149146825410064 2117 2 -0.053789752293049276 2117 3 0.654046960151554 2117 4 -0.2098543718782011 2117 5 -0.2298658977597512 2117 6 -0.0964137912451406 2117 7 -0.9699641166846007 2117 8 -3.177621005060516 2117 9 0.5333081101380159 2117 10-11 -0.04347859207981486 2117 12-13 0.783175056819502 2117 14-15 0.19320251724585802 2117 16-17 -0.011858721623525526 2117 18-19 0.560030701643278 2117 20-21 0.08346094673819238 2117 22-23 1.7485248849800996 2117 24-25 0.5553935439106041 2117 26-27 1.009316129176696 2117 28-29 1.3435027001127509 2117 30-31 0.007467390598932866 2117 32-33 1.343815406717951 2117 34-35 0.18376981762995825 2117 36-37 -2.119916895743458 2117 38-39 0.5430726012926002 2117 40-41 -0.31543466709442214 2117 42-43 0.2664279250517225 2117 44-45 1.197335224825249 2117 46-47 -0.2202258423404757 2117 48-49 0.03316840690681033 2117 50-51 -0.05636698843687071 2117 52-53 0.13416323720640833 2117 54-55 -0.2729273384289215 2117 56-57 -0.32235540940914476 2117 58-59 0.19409399320250031 2117 60-61 0.8053043843138141 2117 62-63 0.3445393584421268 2117 64-65 -0.17959605917975097 2117 66-67 -0.18685644098814436 2117 68-69 0.4962693837834671 2117 70-71 0.3228483708489698 2117 72-73 0.38026307548327765 2117 74-75 0.20780104645367103 2117 76 -0.869576402616353 2118 1 0.31089176907189753 2118 2 0.045713969791318476 2118 3 0.9215407566776079 2118 4 1.4541654792384229 2118 5 0.9443276506273435 2118 6 0.9388219407647824 2118 7 0.3957927071367422 2118 8 -4.764221501338433 2118 9 -0.8403891603334444 2118 10-11 -1.53777139605004 2118 12-13 0.426847513395181 2118 14-15 -1.37702080781618 2118 16-17 -0.4870448258418776 2118 18-19 -0.6694978343368732 2118 20-21 -3.249169326214659 2118 22-23 -0.5761649413226309 2118 24-25 -0.5482044709777298 2118 26-27 1.125072952998032 2118 28-29 -0.49768836688476625 2118 30-31 -0.8507211950090081 2118 32-33 0.25039108910008423 2118 34-35 0.5486581550989413 2118 36-37 -1.1349293026913507 2118 38-39 -0.30854030193320625 2118 40-41 -1.0038962055559608 2118 42-43 0.015063160783732599 2118 44-45 0.03319130422972094 2118 46-47 -0.2294069837796755 2118 48-49 -3.6279978461949263 2118 50-51 -0.7440841100249607 2118 52-53 -1.8534298149027713 2118 54-55 -0.8791308123743349 2118 56-57 1.5228058809134382 2118 58-59 0.6913644646665205 2118 60-61 -1.097797352658894 2118 62-63 0.7625294328838095 2118 64-65 1.2519175884629234 2118 66-67 -1.626434604759858 2118 68-69 1.4100410959422724 2118 70-71 -1.5890622991262155 2118 72-73 -2.1337567756333513 2118 74-75 -1.158452055283302 2118 76 -0.8951347152714391 2119 1 1.188084751528038 2119 2 1.1898435244471912 2119 3 0.5550090427774741 2119 4 1.2298739812627169 2119 5 0.8601171243115537 2119 6 2.7547463672155246 2119 7 -0.37856626722223297 2119 8 1.4109471896197334 2119 9 1.0154812850106865 2119 10-11 1.1328089008460438 2119 12-13 0.580288808941738 2119 14-15 -0.021285315238579017 2119 16-17 0.008231827329503005 2119 18-19 1.499193123827773 2119 20-21 -0.4480897040824061 2119 22-23 1.792526016842011 2119 24-25 0.8634014669439978 2119 26-27 1.2103631014460774 2119 28-29 1.3282225642893195 2119 30-31 1.0888199655847828 2119 32-33 0.9599727355238414 2119 34-35 0.6072276557737055 2119 36-37 1.116217795824177 2119 38-39 1.0388281191194224 2119 40-41 0.8117583153617147 2119 42-43 -0.2148963533863011 2119 44-45 -0.5433269143937629 2119 46-47 -0.6296768893127478 2119 48-49 0.9096539756674176 2119 50-51 1.464148008733467 2119 52-53 -0.728598505860937 2119 54-55 -0.5389148879478824 2119 56-57 0.7701743019660654 2119 58-59 1.1261822784317062 2119 60-61 1.2907316621859124 2119 62-63 0.9711664099418442 2119 64-65 0.8365329730783131 2119 66-67 1.402985098344061 2119 68-69 0.3385161296804675 2119 70-71 0.7139066617374787 2119 72-73 -1.7129740495874657 2119 74-75 0.553018929871893 2119 76 1.4187654196813249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 51.0 14 145.0 15 315.0 16 448.0 17 466.0 18 492.0 19 498.0 20 417.0 21 367.0 22 281.0 23 272.0 24 253.0 25 264.0 26 328.0 27 377.0 28 459.0 29 502.0 30 612.0 31 743.0 32 999.0 33 1333.0 34 1745.0 35 2651.0 36 4287.0 37 5369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.232951886672055 23.12928063980942 10.724465052962948 33.91330242055558 2 15.820592955486104 20.20440915617873 39.04890615761467 24.9260917307205 3 14.595827350282963 25.4582312695329 33.4698876594307 26.47605372075344 4 10.803277303826336 22.269617366331616 37.84525720077709 29.08184812906495 5 12.577075766534335 37.90438381620069 34.12450375876341 15.39403665850156 6 23.173409916378073 38.82084635526649 17.404341582903964 20.601402145451473 7 23.502829630880985 33.99780386856998 20.77455866204916 21.724807838499874 8 19.832756144944675 38.28448348678098 18.996536869668045 22.886223498606302 9 22.22316073992736 17.505701495058705 21.032181772109134 39.238955992904806 10-11 22.630712053382886 26.216318945856916 27.255258045443025 23.897710955317173 12-13 21.125095024917645 24.556550384323 26.260663907424615 28.05769068333474 14-15 19.20770335332376 25.808767632401384 24.457302137004813 30.52622687727004 16-17 21.24968325027452 27.924655798631644 25.306191401300786 25.519469549793055 18-19 21.287693217332546 26.29445054480953 25.0739082692795 27.343947968578426 20-21 20.13050088689923 27.018751583748628 25.650392769659604 27.20035475969254 22-23 22.237942393783257 26.754793479178986 25.840442604949743 25.16682152208801 24-25 21.805051102289045 28.796773376129742 24.30737393360926 25.090801587971956 26-27 19.203480023650645 28.826336683841543 26.4190387701664 25.551144522341414 28-29 20.683757074077203 27.67336768308134 27.728270968831826 23.91460427400963 30-31 20.994171805051103 27.411521243348258 27.4685361939353 24.125770757665343 32-33 19.48221978207619 29.288791283047555 26.678773545062928 24.550215389813328 34-35 20.46414393107526 28.040797364642284 26.843483402314384 24.65157530196807 36-37 20.916040206098486 27.59523608412873 26.913168341920766 24.575555367852015 38-39 21.21800827772616 27.588901089619057 27.428414562040714 23.764676070614073 40-41 20.65630543120196 26.511952022974917 27.901427485429515 24.930315060393614 42-43 20.240307458400203 26.33668384154067 28.184390573528166 25.23861812653096 44-45 18.814933693724132 26.799138440746685 28.729200101359915 25.656727764169275 46-47 19.972126024157447 26.921615001266996 28.015457386603597 25.090801587971956 48-49 19.56457471070192 27.624799391840526 28.08091899653687 24.729706900920686 50-51 19.712391249260914 27.677591012754455 27.10532984204747 25.504687895937156 52-53 18.814933693724132 27.51076949066644 27.580454430272827 26.0938423853366 54-55 18.46862066052876 27.217248078384998 28.074584002027198 26.239547259059044 56-57 19.433651490835373 27.36928794661711 27.067319874989444 26.129740687558073 58-59 19.114790100515247 27.428414562040714 27.177126446490412 26.279668890953626 60-61 19.581468029394376 26.621758594475885 26.95328997381536 26.843483402314384 62-63 19.361854886392432 26.716783512120955 26.93428499028634 26.987076611200273 64-65 19.469549793056846 27.12011149590337 26.41059211082017 26.999746600219616 66-67 19.332291578680632 27.757834276543626 25.88056423684433 27.029309907931413 68-69 19.173916715938848 27.493876171973987 25.698961060900416 27.633246051186756 70-71 19.97634935383056 28.881239969592027 24.725483571247572 26.41692710532984 72-73 19.885547765858604 27.694484331446912 25.16893318692457 27.25103471576991 74-75 19.439986485345045 28.570825238618124 25.0147816538559 26.97440662218093 76 20.652082101528848 26.573190303235073 26.543626995523272 26.231100599712814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 0.5 14 0.0 15 0.0 16 0.5 17 1.0 18 1.5 19 2.5 20 4.0 21 5.0 22 5.5 23 12.5 24 19.0 25 19.0 26 33.5 27 62.0 28 92.0 29 108.0 30 125.5 31 173.5 32 271.5 33 339.0 34 369.5 35 504.0 36 662.0 37 716.0 38 829.0 39 1065.0 40 1300.5 41 1444.0 42 1475.0 43 1562.0 44 1736.5 45 1859.0 46 1894.0 47 1837.5 48 1694.5 49 1531.0 50 1454.0 51 1367.0 52 1156.5 53 936.5 54 840.0 55 769.0 56 584.0 57 431.0 58 392.0 59 323.5 60 226.0 61 163.5 62 130.0 63 133.0 64 102.5 65 68.5 66 55.0 67 42.0 68 35.0 69 26.0 70 22.5 71 21.0 72 16.5 73 11.0 74 9.5 75 9.0 76 6.5 77 6.5 78 6.5 79 4.0 80 3.5 81 2.5 82 2.5 83 3.0 84 1.5 85 0.5 86 1.0 87 1.0 88 2.0 89 2.0 90 2.0 91 2.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7221893741025425 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 23678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.09274431962159 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29808348322273 53.315313793394715 2 10.829250139552192 14.747867218515076 3 5.07969980772809 10.376721006841795 4 2.437511629349377 6.639074246135654 5 1.3955219252000248 4.751245882253569 6 0.8869317124604602 3.623616859532055 7 0.36593686038578427 1.7442351549961992 8 0.30391366371022766 1.6555452318607988 9 0.14265335235378032 0.8742292423346567 >10 0.26049742603733794 2.2721513641354845 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT 32 0.13514654953965705 No Hit GGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG 29 0.12247656052031422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008446659346228567 0.0 2 0.0 0.0 0.0 0.008446659346228567 0.0 3 0.0 0.0 0.0 0.01266998901934285 0.0 4 0.0 0.0 0.0 0.021116648365571415 0.0 5 0.0 0.0 0.0 0.0253399780386857 0.0 6 0.0 0.0 0.0 0.0253399780386857 0.0 7 0.0 0.0 0.0 0.0253399780386857 0.0 8 0.0 0.0 0.0 0.03800996705802855 0.0 9 0.0 0.0 0.0 0.0506799560773714 0.0 10 0.0 0.0 0.0 0.0760199341160571 0.0 11 0.0 0.0 0.0 0.08024326378917138 0.0 12 0.0 0.0 0.0 0.08024326378917138 0.0 13 0.0 0.0 0.0 0.08024326378917138 0.0 14 0.0 0.0 0.0 0.09713658248162851 0.0 15 0.0 0.0 0.0 0.10558324182785708 0.0 16 0.0 0.0 0.0 0.11402990117408565 0.0 17 0.0 0.0 0.0 0.1562631979052285 0.0 18 0.0 0.0 0.0 0.18160317594391417 0.0 19 0.0 0.0 0.0 0.18160317594391417 0.0 20 0.0 0.0 0.0 0.19427316496325703 0.0 21 0.0 0.0 0.0 0.2027198243094856 0.0 22 0.0 0.0 0.0 0.20694315398259988 0.0 23 0.0 0.0 0.0 0.2322831320212856 0.0 24 0.0 0.0 0.0 0.24072979136751416 0.0 25 0.0 0.0 0.0 0.24917645071374273 0.0 26 0.0 0.0 0.0 0.253399780386857 0.0 27 0.0 0.0 0.0 0.26606976940619986 0.0 28 0.0 0.0 0.0 0.29563307711799985 0.0 29 0.0 0.0 0.0 0.3209730551566855 0.0 30 0.0 0.0 0.0 0.3758763409071712 0.0 31 0.0 0.0 0.0 0.41388630796519976 0.0 32 0.0 0.0 0.0 0.4645662640425712 0.0 33 0.0 0.0 0.0 0.49835290142748545 0.0 34 0.0 0.0 0.0 0.565926176197314 0.0 35 0.0 0.0 0.0 0.6588394290058281 0.0 36 0.0 0.0 0.0 0.7390826927949996 0.0 37 0.0 0.0 0.0 0.9080158797195709 0.0 38 0.0 0.0 0.0 1.178308978798885 0.0 39 0.0 0.0 0.0 1.5330686713404849 0.0 40 0.0 0.0 0.0 2.027198243094856 0.0 41 0.0 0.0 0.0 2.521327814849227 0.0 42 0.0 0.0 0.0 2.9732240898724553 0.0 43 0.0 0.0 0.0 3.488470309992398 0.0 44 0.0 0.0 0.0 3.9530365740349693 0.0 45 0.0 0.0 0.0 4.4218261677506545 0.0 46 0.0 0.0 0.0 4.894839091139454 0.0 47 0.0 0.0 0.0 5.380522003547597 0.0 48 0.0 0.0 0.0 5.921108201706225 0.0 49 0.0 0.0 0.0 6.554607652673368 0.0 50 0.0 0.0 0.0 6.985387279331024 0.0 51 0.0 0.0 0.0 7.437283554354253 0.0 52 0.0 0.0 0.0 8.049666356955823 0.0 53 0.0 0.0 0.0 8.514232620998396 0.0 54 0.0 0.0 0.0 8.945012247656052 0.0 55 0.0 0.0 0.0 9.388461863333053 0.0 56 0.0 0.0 0.0 9.755891544893995 0.0 57 0.0 0.0 0.0 10.148661204493623 0.0 58 0.0 0.0 0.0 10.663907424613566 0.0 59 0.0 0.0 0.0 11.158036996367937 0.0 60 0.0 0.0 0.0 11.597263282371822 0.0 61 0.0 0.0 0.0 12.091392854126193 0.0 62 0.0 0.0 0.0 12.437705887321565 0.0 63 0.0 0.0 0.0 12.746008953458906 0.0 64 0.0 0.0 0.0 13.096545316327392 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTA 15 0.0021998286 69.9852 46 TTACCAT 15 0.0021998286 69.9852 54 ACGCATC 15 0.0021998286 69.9852 13 ATTGGCC 15 0.0021998286 69.9852 68 TACGCAT 15 0.0021998286 69.9852 12 ACGATCC 15 0.0021998286 69.9852 42 AAAGTCC 15 0.0021998286 69.9852 29 GCATCCT 15 0.0021998286 69.9852 15 TCCCTAC 15 0.0021998286 69.9852 8 CGATCCC 15 0.0021998286 69.9852 43 TTATAAT 25 2.3457606E-4 55.98816 19 GGTAGGT 25 2.3457606E-4 55.98816 49 TGGGTAG 25 2.3457606E-4 55.98816 47 GTTATAA 25 2.3457606E-4 55.98816 18 ACTATGT 25 2.3457606E-4 55.98816 29 GATGGGT 20 0.00687431 52.4889 45 CTTTTAA 20 0.00687431 52.4889 37 TGTTGGG 20 0.00687431 52.4889 61 TCCCATC 20 0.00687431 52.4889 3 CCTACGT 20 0.00687431 52.4889 34 >>END_MODULE