FastQCFastQC Report
Wed 25 May 2016
SRR1780678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23678
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT7793.2899738153560265No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT3711.5668553087253991Illumina PCR Primer Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC990.41810963763831405No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC780.32941971450291413No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG710.29985640679111414No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT460.19427316496325703No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT340.14359320888588561No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT290.12247656052031422No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT270.11402990117408565No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA260.10980657150097137No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC240.1013599121547428No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTG150.002197984870.037
AGCTTCA200.006868564552.56
ACTCTGT200.006868564552.538
ATCGGGG200.006868564552.533
CCCGATG200.006868564552.513
TGCTTGA2100.033.33333255
GCTTGAA2100.033.33333256
GTCTTCT2150.032.5581449
CTGCTTG2150.032.5581454
TCTTCTG2150.032.5581450
CTTCTGC2150.032.5581451
TTGAAAA2050.032.43902658
TTCTGCT2200.031.81818452
CCGTCTT2200.031.81818447
TCTGCTT2200.031.81818453
CGTCTTC2200.031.81818448
CTTGAAA2100.031.66666657
GTATGCC2250.031.1111142
TGCCGTC2250.031.1111145
TATGCCG2250.031.1111143
Produced by FastQC (version 0.11.5)