FastQCFastQC Report
Wed 25 May 2016
SRR1780677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGACCCGGGCGACGGCCACGCGGACCACGAGGCCTGTCTCTTATAC11.0752688172043012No Hit
CGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCT11.0752688172043012No Hit
CGTTCGGCCCGATCGCGGAGCCGGCGCCGCGGGCGCCGTAGCCCAGGTGC11.0752688172043012No Hit
CTAATAACCTATTCCAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATA11.0752688172043012No Hit
AAAGACTAGTATGGCAGTAGGCGCAATATTGGCTAAGAGGGAGTGGGTGT11.0752688172043012No Hit
GCCCACCGCCGTCGTCGAAGGTAGACCAATCCCTGTTCGCTCATGGGGCG11.0752688172043012No Hit
GGGCTATTGGTTGAANGNNNNGGNTGATGGTTTCGATAANAACTAGTATG11.0752688172043012No Hit
CCAGCCCGGAGAGAAACGCTGGAGAATCACCTCGGTTTCTTTCTCAGTAA11.0752688172043012No Hit
GGTGCGACCCGTGGGAGGAAGGCGGGGGGAGAAGCGGCGAAGGTTAGGGA11.0752688172043012No Hit
GAGGATTCCGCGTCACAGGAGTTGGGCGCATACGAACTGGGGATTCGTGT11.0752688172043012No Hit
GCGCCAGGTGGGCGCGCTGTGGAACGCGCTCGACCCGCGCCAGACCGCGG11.0752688172043012No Hit
GTGTAGCCGACCCGCCGGCCAGGCCCGCGCTTGGAACTGTCTCTTATACA11.0752688172043012No Hit
ACAACACAGGCCCAATCACAGCACACTGCATCTGGTTTGAACGGTAAACC11.0752688172043012No Hit
CGAACTAAGTGACGTGAACCCCGACAGCGGCGGCGACCACTCATCCGACG11.0752688172043012No Hit
GGCGGGGGGGGGTGGAGGGAGTGGGTATAGTAGAAGGGAAAGTAAGGGAA11.0752688172043012No Hit
GAAACACTGCATTTATTTTGTGTTACACACCATCTTATCACCTGCCTCTT11.0752688172043012No Hit
GTGCCCATGCGTGCGGCCAGGCTCTTCGTGCTCAGCGGCGCGTCTCCCCA11.0752688172043012No Hit
GGGCGTGCTCCACAGTCAGAATCCGTGCCGATGACCAAACCGAAGTAACC11.0752688172043012No Hit
CTGACGGGGCGCGGTTGGTACCCCAAGTGTCCTGGCGGAATATGGAGGGC11.0752688172043012No Hit
CCCGGGCCGCGACCGCCGCCACCTCCCGCCGCGCCGTCGCCGAGGCTGTC11.0752688172043012No Hit
GTGCCGGAGTGGGCCTTCTCGCCGTCGAGGCGGCGCTGCAGGTCGATCGC11.0752688172043012No Hit
CCTATACACACCTGACGCTGCCCCCGCGACGCTGCGTCGAGCTCTCGGTG11.0752688172043012No Hit
ATACATAGCGGTTGTTGATGGGTGAGTCAATACTTGGGTGGTACCCAAAT11.0752688172043012No Hit
GGTCGTGCTCTATGGAGCCATCCAAATAAGTCGGCGGTTTGAAACGATAG11.0752688172043012No Hit
TAGAGAAAGAATAGAGGATTACTAAGCATGGAGAAACTCCAAGCAGAGGT11.0752688172043012No Hit
CCCCTAAGCTTGCCTCCCGCCACGAAAGCTGGAAAGTCACTGGGGGCGGA11.0752688172043012No Hit
AACGCTACCCCGACCGGGGCCTGAAGGCGTGGTAGGGCTGTCTTCTTGTC11.0752688172043012No Hit
CTGTTCTTTCATGGGGAAGCAGATTTGGGTACCACCCAAGTATTGACTCA11.0752688172043012No Hit
CTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTTTTGATGACTTCTA11.0752688172043012No Hit
AGCTGGTAAGGGAGAGAATATCCGGCTACCATACGGGGCGGCGGGGTGGC11.0752688172043012No Hit
CGCACGCCACCGCCTCCATCACCCTTCTCCTAGATCTCCTCTGCACTCAC11.0752688172043012No Hit
CGACCCGGTGTGCCGCTAGTGGCGTTTTAGCTAAAACAGAGGTGTGTACG11.0752688172043012No Hit
AGCCTGAGGCATGGGGGTCAGGGGTTAAGGGCTGGGAGGGTGTTTGCGTA11.0752688172043012No Hit
CGAGTGGGCCGGCTACGACCGCGAGTTCGAGGAGGCCGACGTGGCCGAGC11.0752688172043012No Hit
CGGTCTCGAAACTTTCTGAACACGCGATCCTGAGAGCGGAGAGGTCCACA11.0752688172043012No Hit
CACCGGGCACGTTCAGACGCTCGGCCCGCTGCGCGCGCTTCCAGCGCCGC11.0752688172043012No Hit
TCCTTGCGTCTCGCGACCCTTGCGTCCAGTCCCTTCTCTTCATCCCCCTT11.0752688172043012No Hit
TCGTTGCCTCCCGAGGCCGAGCGGATGGAGAAGGCGGAGGCCGCGAGCAC11.0752688172043012No Hit
AGGGTAGCCCATGGCGGGGTCCAGGGCGACGCCGTCGCGAATGGGGATGG11.0752688172043012No Hit
GCCCGGGTCCGCTCACCATTCGCGACCAACGCCGCACTCCTCCCACCCCC11.0752688172043012No Hit
GAATGTTGAGTCCTGTAAGTAGGAGAGTGATATTTGATCAGGAGAACGTG11.0752688172043012No Hit
GTACATAACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATT11.0752688172043012No Hit
ACCGCGGACCTCGCCCGCTCCGGCGACGAGTACTTCTACGCGTTCGAGGG11.0752688172043012No Hit
CGGGTTCCGGTGGGGAAGCTAGGTGCGGTGCATGAGGAGGTCTGGGTACC11.0752688172043012No Hit
TGTCCAGAGTAGGAGAGAGAAAGGGGAAGGGGGGGGAGAATGTTTATTAT11.0752688172043012No Hit
GGCTACATTCCTGAGACCCTGAGAAAAAGTACCTGCATAAGACCTGTCTC11.0752688172043012No Hit
CTTATACAGATGTGTCGCTGCGGACGACCAAGCCCGTGTAGACTGCTGTG11.0752688172043012No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA11.0752688172043012No Hit
TGCGCGGAGCCGACGGCAAAGACGAGCTGTGCTGCCTCCAGGCGCGGAGA11.0752688172043012No Hit
TAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCA11.0752688172043012No Hit
GGACGGCGTCTGGGTNTNNNNGGACACCGGGGAGACTGTNTCTTATACAC11.0752688172043012No Hit
CTTACGATGGGAGCGGGGTTTGGGGGGGGGGTGTAGCCGAATGTTAGTTG11.0752688172043012No Hit
CTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTTTTGATGACTTC11.0752688172043012No Hit
CACGCTGCACCGGGTACAAACTCCGAGACTTCCGAAAAAGGTGGGTATGC11.0752688172043012No Hit
TTCCAATTCTGTGAAGGAAGGGAGTGGGAGCATGATGGGGATGGCATTGA11.0752688172043012No Hit
ATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGAAGTATAGTAC11.0752688172043012No Hit
AGTGAGGGTGGGAGTGGGGTGGGGGGTGTGTCGAGGGTGGCTCGTCGTGG11.0752688172043012No Hit
CTTATACACATCTGACGCTGCCGACGAGACCGGCTGTGTAGATCTCGGTG11.0752688172043012No Hit
GGTGAGGTTGATCGGGGTTTATCGATTACCGAACAGGCTCCTCTAGAGGG11.0752688172043012No Hit
ATACACATCTGACGCTGCCGACGAGAACGTCCGGGGAGATCTCCGGGGGC11.0752688172043012No Hit
CAGCCTAAAGTGTGGGCAAAAGTGTTCAGCCACCATGCTCGGCCCTAACT11.0752688172043012No Hit
GTATGGCAATAGGCACAATATTGGCTAAGAGGGAGTGGGTGTTGAGGGTT11.0752688172043012No Hit
CTTATACCCATCTGACGGTGCCGACGGGACCCAATCTTTAGATCTCTATG11.0752688172043012No Hit
ACACGTCTGACGCTTCCGGCGAGACGCAAAGGGTAGATTCGGGGGATCTC11.0752688172043012No Hit
CGGTCAGCTGCGGCGGGGGGGGGGGGGGGGGTGTAGGGGGGGGGGGGGGG11.0752688172043012No Hit
GTCTTGTAAACCGAAGATGAAAACCTTTTTCCAAGGACAACTCAGAGAAA11.0752688172043012No Hit
GATTAGTATAGAGCGGTAGAGTTTTTTTCGTGATAGTGGTTCACTGGATA11.0752688172043012No Hit
GAGGCGAGGCGCTCCGGGGGGGTCGTGGCGGGGGAGAGTAGGAGGCCCGA11.0752688172043012No Hit
GCCCAGGAAAGCACCAAGAAGACTCTACTTGACTGTGACTTCCCCCTCCA11.0752688172043012No Hit
CTGCTGTTTCCCGTGGGGGGGTGGCTAGGCTAAGCGTTTTGAGCTGCATT11.0752688172043012No Hit
ATAGACATCTGACGCCCCCGACGCGAGGAGGCCTGATGATTTGCGCTTAT11.0752688172043012No Hit
GTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCCTG11.0752688172043012No Hit
CGGGCGGGGAGCCCGCGGTGACGCGCCCCGGCTTCCGTCCTGTCTCTTAT11.0752688172043012No Hit
AAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACCTGTCTCTTAT11.0752688172043012No Hit
CCCTTCACGCCTGCCAGCTCCGCCGGTTCCCCCCCTCTTAGATCCTTCGT11.0752688172043012No Hit
CTTTGGGAGGCCGAGGTGGGCGGGTCACAAGGTCAGGCTGTCTCTTATAC11.0752688172043012No Hit
TCTTAATACACATGTGACGCTGCCGAAGACCAAGCCCGTGTAGATGTCGG11.0752688172043012No Hit
GAAGCAGGGGAATTGCTTGCACCCCGGCGGCGGAGGTTGCAGTGGTCAAC11.0752688172043012No Hit
CCCTAATCCTGCCTGTCAAGAAATCAGATGGGTCATATCGATTACTGCAA11.0752688172043012No Hit
CTTAACACATCTGGGGCTGCCGGCGAGAACGGCGGTGTCGCTCTCGGTGG11.0752688172043012No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTG11.0752688172043012No Hit
CCGGGGGGGGCGGCGGGCGGGGGGGGGGGGGGGCGTGGGGTGGGGGGGGG11.0752688172043012No Hit
ACCTCCATCATCACCTCAACCCAAAAAGGCATAATTAAACTTTACTTCCT11.0752688172043012No Hit
CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCA11.0752688172043012No Hit
TGATATGTTTGCGGTTTCGATGATGTGGTCTTTGGAGTAGAAACCTGTGA11.0752688172043012No Hit
GTCCGCTCCATACCCACGCCGGCAGAAAATAGGCATCGCATGGGACGCGG11.0752688172043012No Hit
GCAGCCCCTCAGGTCCCGTGTTCGGGGGCGGCCGGGGGGATATGGGACCG11.0752688172043012No Hit
GGCCTATGAGTGACTACAAAAAGGATTAGACTGAACCGAATTGGTATATA11.0752688172043012No Hit
GTACGGAACCTGCGCGTGCATCCCCTACGGCTGGGGGGTGTGCGACCCCG11.0752688172043012No Hit
AAAATACAGTCCATGGCAATTACTATTAATACCTCCCATCATCTCTCATT11.0752688172043012No Hit
GCTTGACGGCGCCCACCTTGGCGGACATGCCGTACTCGGTGACCATCTTG11.0752688172043012No Hit
TTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGA11.0752688172043012No Hit
GGCGCACTGATCCATTCCGCTAGCCGGCTTAAAAGAGGTGACATCTGGAC11.0752688172043012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTC50.044.028
GATGGGT50.044.027
ATCCTGC50.044.06
ACTGCAA50.044.044
AATCCTG50.044.05
CAAGAAA50.044.017
GGGTCAT50.044.030
GTCATAT50.044.032
TCGATTA50.044.038
TCAAGAA50.044.016
GAAATCA50.044.020
AAATCAG50.044.021
TTACTGC50.044.042
CCTAATC50.044.02
CCTGCCT50.044.08
CCTGTCA50.044.012
AATCAGA50.044.022
GTCAAGA50.044.015
TATCGAT50.044.036
TGTCAAG50.044.014