FastQCFastQC Report
Wed 25 May 2016
SRR1780677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3234.40860215053764No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC44.301075268817205No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT33.225806451612903TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG33.225806451612903No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC11.0752688172043012No Hit
CTCCCTCAGGGGAAAGACCCCGGCGGGGGCAACGCCCAAAATTTGCTTTC11.0752688172043012No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGGGGTTTGGTGGAAATTTTTTGT11.0752688172043012No Hit
GATCGTGTTCAAGGACCCGTCCACGGGCGCCGCGAACGACATCCAGAAGG11.0752688172043012No Hit
GTTATGAAATGGTTTTTCTAATACCTTTTTGAAAAAGTCATGGAGGCCAT11.0752688172043012No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT11.0752688172043012No Hit
GCCTCGTGGTCCGCGTGGCCGTCGCCCGGGTCGTGGGCTGTCTCTTATAC11.0752688172043012No Hit
CTCTCATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC11.0752688172043012No Hit
CTTATACACATCTCCGACACGAGACCGGTTCCCATCTCGTATGCCGTCTT11.0752688172043012RNA PCR Primer, Index 14 (95% over 21bp)
GCCATCTACCGTACATAGCACATTACAGTCAAATCCCTTCTCGTCCCCAT11.0752688172043012No Hit
AAAGGTAACAAGGATATCCAGGAATTGAACTCAGCTCTGCATCAAGTGGA11.0752688172043012No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTACGATCTCGTATGCCGT11.0752688172043012No Hit
CTCGAGATCCCGCCGCACCTGGGCTACGGCGCCCGCGGCGCCGGCTCCGC11.0752688172043012No Hit
ATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAG11.0752688172043012No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTCTGTCTCTTAT11.0752688172043012No Hit
GTGATAAGATGGGGTGTAACACAAAATAAATGCAGCGTTTCCTGTCTCTT11.0752688172043012No Hit
GTCCTCGACGGCGAGGTCGCCGCCGGTGCGCAGGCCCTCGGCGCGATCGA11.0752688172043012No Hit
GTCCAAGGTAATTTACAGATTCAATGCCATCCCCATCAAGCTACCAATGA11.0752688172043012No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA11.0752688172043012No Hit
GACGGAAGCCGGGGCGCGTCACCGCGGGCTCCCCGCCCGCTGTCTCTTAT11.0752688172043012No Hit
GGTGTAAGGGTTAGGAACAATGGGGTGAGTGGTCTGTACTATTTGATTAA11.0752688172043012No Hit
TCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCTTG11.0752688172043012Illumina PCR Primer Index 11 (96% over 29bp)
GTGTTAATGTCATTAAGGAGAGAATGAAGAGAAGTAAGCCGAGGGCGTCT11.0752688172043012No Hit
CAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAACCCCCTGAA11.0752688172043012No Hit
CTGCACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT11.0752688172043012TruSeq Adapter, Index 9 (95% over 21bp)
TATACACATCTCCGGGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT11.0752688172043012Illumina PCR Primer Index 11 (95% over 21bp)
CTCATATCCTCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCAT11.0752688172043012No Hit
GTCTCTATAATCTCACCCGGGGGGACCCGACCTCAGGGATCCCCTTAAAC11.0752688172043012No Hit
ATACACATCTCCGAGCCTCCGAGCCCACGAGACCTCATGGGATCTCGTAT11.0752688172043012No Hit
GCAGACTGCTGCGAACAGAGTGGTGATAGCGCCTAAGCATAGTGTTAGAG11.0752688172043012No Hit
CACCCGTACTCTAGAAAGAACCCTAGGAAGAGTTTAGGCGGGCGCGCAGG11.0752688172043012No Hit
GGATAATATAAATAGTTAAATTAAGAATGGTTATGTTAGGGTTGTACCTG11.0752688172043012No Hit
CTTATACACATCTCCGAGCCCACCATCTCGTATGCCGTCTTCTGCTTGAA11.0752688172043012RNA PCR Primer, Index 1 (96% over 28bp)
TACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTTC11.0752688172043012RNA PCR Primer, Index 22 (95% over 23bp)
ACCTAACTCTTTTTTGGTTCTCCTCCTACCTCACTGCCTGTTCCCTGTCA11.0752688172043012No Hit
CCTCGGCGACGGCGCGGCGGGGGGGGGGGGCGGGCGCGGGCCGGGGCTTC11.0752688172043012No Hit
AACCCTGGGAGGCGAACGGTCGCGGCCCCGGGCCGGTGAGGACCGGCCGG11.0752688172043012No Hit
GCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAA11.0752688172043012No Hit
TTCCAAGCGCGGGCCTGGCCGGCGGGGCGGCTACACCTGTCTCTTATACA11.0752688172043012No Hit
GTGCTCACCGTGCTCGCGGCCTCCGCCTTCTCCATCCGCTCGGCCTCGGG11.0752688172043012No Hit
CTAATACACCAGTCTTGTAAACCGAAGATGAAAACCTTTTTCCAAGGACA11.0752688172043012No Hit
TCTTATACACATCTCCGAGCCTACGAGACGACGCTCCATCTCGTATGCCG11.0752688172043012No Hit
GGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGAGCAGA11.0752688172043012No Hit
GTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGCTAATAATTAGGC11.0752688172043012No Hit
GTCTTATGCAGGTACTTTTTCTCAGGGTCTCAGGAATGTAGCCCTGTCTC11.0752688172043012No Hit
CGCCTACACAATTCTCCGATCCGTCCCTAACAAGCTAGGAGGCGTCCTTG11.0752688172043012No Hit
CCTCAACGGCACCCTCTACGCGAACCGGCTCGAGGGCAAGTGGCAGCGGC11.0752688172043012No Hit
ATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAAT11.0752688172043012No Hit
CTTATACACATCTCCGAGCCCCGAGACGACGCTCCATCTCGTATGCCGTC11.0752688172043012No Hit
TCTCCCCGGTGTCCGGGCACACCCAGACGCCGTCCCTGTCTCTTATACAC11.0752688172043012No Hit
AGCACAAACCCGCCGAAGCCCCCGCGACCTTGCCGAGCGGGGGTCCGGAC11.0752688172043012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTG50.044.030
ATGGGGT50.044.020
AACAATG50.044.016
GGTGAGT50.044.024
CAATGGG50.044.018
GGGTTAG50.044.08
TAGGAAC50.044.012
GAGTGGT50.044.027
GTACTAT50.044.036
GTGTAAG50.044.02
GGTCTGT50.044.031
AGTGGTC50.044.028
AGGGTTA50.044.07
TGATTAA50.044.044
GTCTGTA50.044.032
AGGAACA50.044.013
AAGGGTT50.044.06
GTGAGTG50.044.025
TGGGGTG50.044.021
GGTTAGG50.044.09