FastQCFastQC Report
Wed 25 May 2016
SRR1780676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94988
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG150.00221966669.9915719
CCGCGTG150.00221966669.991579
ACATCCA150.00221966669.9915719
ATCCTTA252.3812595E-455.99326342
AAGACGA252.3812595E-455.99326329
CCTATGG252.3812595E-455.99326342
GCATGAC252.3812595E-455.99326317
CCCCTTG200.00693581152.4936831
GACTTCC200.00693581152.4936823
ACGCTTA200.00693581152.4936859
CCCTTGT200.00693581152.4936832
CGTCCTT200.00693581152.493684
CGCTTAG200.00693581152.4936860
GCTCAAA200.00693581152.4936852
CTATGGA305.855519E-446.6610543
GAGCTTT305.855519E-446.6610548
GAAGACC305.855519E-446.6610536
CTGACTT305.855519E-446.6610521
AATTCCC305.855519E-446.6610532
TTCGGTC305.855519E-446.6610533
Produced by FastQC (version 0.11.5)