##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94988 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.65957805196446 34.0 31.0 34.0 12.0 34.0 2 30.967343243357057 34.0 32.0 34.0 23.0 34.0 3 31.38124815766202 34.0 33.0 34.0 23.0 34.0 4 31.747315450372678 34.0 34.0 34.0 23.0 34.0 5 31.36427759295911 34.0 33.0 34.0 23.0 34.0 6 34.50648502968796 38.0 35.0 38.0 26.0 38.0 7 33.70816313639617 37.0 34.0 38.0 21.0 38.0 8 32.7330083800059 37.0 32.0 38.0 11.0 38.0 9 33.54852191855814 37.0 34.0 38.0 21.0 38.0 10-11 33.95674190424053 37.0 34.0 38.0 22.5 38.0 12-13 34.325025266349435 38.0 34.5 38.0 24.5 38.0 14-15 33.9090358782162 37.0 34.0 38.0 22.5 38.0 16-17 33.74935781361856 37.0 34.0 38.0 21.5 38.0 18-19 33.75312144691961 37.0 34.0 38.0 22.0 38.0 20-21 33.82018781319746 37.0 34.0 38.0 22.0 38.0 22-23 33.811218259148525 37.0 34.0 38.0 22.0 38.0 24-25 34.27393986608835 38.0 34.5 38.0 23.5 38.0 26-27 34.3405851265423 38.0 34.5 38.0 23.5 38.0 28-29 34.47341769486672 38.0 35.5 38.0 24.5 38.0 30-31 34.33738998610351 38.0 35.0 38.0 24.0 38.0 32-33 34.06086031919821 38.0 34.0 38.0 23.0 38.0 34-35 33.89018086495136 37.0 34.0 38.0 23.0 38.0 36-37 33.899761022444935 37.0 34.0 38.0 22.5 38.0 38-39 34.45936328799428 38.0 35.0 38.0 24.5 38.0 40-41 33.91194677222386 37.5 34.0 38.0 23.0 38.0 42-43 34.25177917210595 37.0 34.5 38.0 24.0 38.0 44-45 33.25336358276835 37.0 34.0 38.0 21.0 38.0 46-47 34.00725881163936 37.0 34.0 38.0 23.0 38.0 48-49 33.737329978523604 37.0 34.0 38.0 22.5 38.0 50-51 33.69750915905167 37.0 34.0 38.0 22.0 38.0 52-53 33.70192550637975 37.0 34.0 38.0 22.5 38.0 54-55 33.411346696424815 37.0 34.0 38.0 21.0 38.0 56-57 33.53357266180991 37.0 34.0 38.0 22.0 38.0 58-59 33.39364446035289 37.0 33.0 38.0 22.0 38.0 60-61 33.41919505621763 37.0 33.5 38.0 21.5 38.0 62-63 33.48578767844359 37.0 33.5 38.0 21.5 38.0 64-65 33.52119215058744 37.0 33.5 38.0 22.0 38.0 66-67 33.44348233461069 37.0 33.5 38.0 22.0 38.0 68-69 33.03828378321472 37.0 32.0 38.0 20.5 38.0 70-71 32.89288646986988 37.0 32.0 38.0 20.0 38.0 72-73 32.992330610182336 37.0 32.0 38.0 20.0 38.0 74-75 32.59418558133659 37.0 31.0 38.0 19.5 38.0 76 31.79541626310692 36.0 30.0 38.0 16.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.775376257497779 1101 2 -1.1116586741278915 1101 3 0.18495251377809296 1101 4 -2.440585306585941 1101 5 -0.6233447693595942 1101 6 -2.786033157790108 1101 7 -2.3808658089483714 1101 8 -1.1723164023098036 1101 9 -0.7457119035902124 1101 10-11 -0.7267772903442449 1101 12-13 -1.8134562035165587 1101 14-15 -0.460037313041461 1101 16-17 -1.58105851293179 1101 18-19 0.007828982508542026 1101 20-21 -1.8163618820186542 1101 22-23 -0.4963186487823279 1101 24-25 -1.2308478149062907 1101 26-27 -1.573790607658573 1101 28-29 -0.3919627748275403 1101 30-31 -2.0696295928616166 1101 32-33 0.43712786826531413 1101 34-35 -0.1297563161997246 1101 36-37 0.31846096527554835 1101 38-39 -0.7434747488073228 1101 40-41 0.25154438541895985 1101 42-43 -0.6324469543320674 1101 44-45 0.01658663093749624 1101 46-47 0.854879476797926 1101 48-49 -0.8212168751539082 1101 50-51 -1.233762920453536 1101 52-53 -0.47031764311611823 1101 54-55 -1.4261125330519668 1101 56-57 -0.8070028462327912 1101 58-59 -0.3496914609750732 1101 60-61 -0.043526042078525506 1101 62-63 -2.1386521511705183 1101 64-65 0.008169922884810887 1101 66-67 -1.3188969540453073 1101 68-69 0.5239593959650506 1101 70-71 0.4282288443963438 1101 72-73 -1.4564852374038608 1101 74-75 -1.105182742372829 1101 76 -2.42626998746114 1102 1 0.5472739736270285 1102 2 -0.7996401841432998 1102 3 0.3934270900492791 1102 4 0.5750541387145205 1102 5 0.4722638592998827 1102 6 0.46227192695565833 1102 7 1.7689801078467013 1102 8 2.1296866793542932 1102 9 0.7330246141293557 1102 10-11 -0.5231178142271489 1102 12-13 -4.234796727399459 1102 14-15 -2.05310356881958 1102 16-17 -3.2631078195573693 1102 18-19 -1.5665932054729623 1102 20-21 0.022659689630032176 1102 22-23 -1.8710490031737024 1102 24-25 -0.015863223226780576 1102 26-27 -6.803220499800336 1102 28-29 -0.3286345776010364 1102 30-31 0.41319012978861736 1102 32-33 -7.056169512320206 1102 34-35 -1.0320290434724555 1102 36-37 0.47824524878865304 1102 38-39 0.4540599199137887 1102 40-41 0.2605197321061752 1102 42-43 0.14652068819489728 1102 44-45 -6.274938792791314 1102 46-47 -0.09708508406493621 1102 48-49 0.1577892881742855 1102 50-51 -6.772014076077571 1102 52-53 -2.3556797386477086 1102 54-55 0.6863682065474137 1102 56-57 -1.1080814286673046 1102 58-59 0.5876351954193879 1102 60-61 0.25755254035598796 1102 62-63 -5.036109778289159 1102 64-65 -1.333856271260025 1102 66-67 -0.29204795558614904 1102 68-69 -8.751888061662072 1102 70-71 -0.7294367950489544 1102 72-73 0.4543776285437531 1102 74-75 -6.895244375654801 1102 76 -2.474498030604437 1103 1 0.12078028865876433 1103 2 0.3665793041100862 1103 3 0.5886204674460451 1103 4 0.9264382104375741 1103 5 0.7731276771128535 1103 6 -0.5750292967862478 1103 7 0.6930197649372047 1103 8 1.3517271217337168 1103 9 1.0429683350900305 1103 10-11 0.9028032642363044 1103 12-13 -0.715246305306664 1103 14-15 -0.12233987534402502 1103 16-17 -0.13428771616099766 1103 18-19 0.7793102039897661 1103 20-21 0.34479992914315716 1103 22-23 0.18200707954339634 1103 24-25 1.0418882328297556 1103 26-27 -0.20788151674948807 1103 28-29 0.7429003350355714 1103 30-31 0.036758613526586714 1103 32-33 -4.497550966413527 1103 34-35 -0.5267201631635743 1103 36-37 0.3277887996033826 1103 38-39 0.9462233908908146 1103 40-41 1.2732888571634362 1103 42-43 1.090192568307458 1103 44-45 -4.743355454004586 1103 46-47 0.4916116635301151 1103 48-49 0.6679898640528279 1103 50-51 0.7335238468332292 1103 52-53 0.5135451157466377 1103 54-55 0.8048895729501453 1103 56-57 1.6275706883407466 1103 58-59 1.1881112768276623 1103 60-61 1.6164950179425261 1103 62-63 0.8045910920727266 1103 64-65 1.1740702387851272 1103 66-67 0.7145533794050252 1103 68-69 -2.9935116465059934 1103 70-71 0.8011403923078859 1103 72-73 1.93844280752419 1103 74-75 -0.2800686672587531 1103 76 0.36091849972734735 1104 1 -2.2595803675639594 1104 2 -0.46676142578236934 1104 3 0.14453105783939435 1104 4 0.5226156686839971 1104 5 -0.6534910598507118 1104 6 -0.40155039916941604 1104 7 1.2386047587980613 1104 8 1.5159455273314322 1104 9 -0.15704941665186567 1104 10-11 -0.5993565310269346 1104 12-13 -0.11084387331802503 1104 14-15 0.08677564411632233 1104 16-17 -0.3972723756973835 1104 18-19 0.5379752729996596 1104 20-21 1.0526210507573808 1104 22-23 0.38732816417482496 1104 24-25 0.7654862150484334 1104 26-27 1.1388617782696642 1104 28-29 -0.6130442795297313 1104 30-31 -1.1628375343454138 1104 32-33 -0.06673838042852509 1104 34-35 0.3458445197459312 1104 36-37 0.11589217391365025 1104 38-39 -0.08462765720160803 1104 40-41 0.9946826647641416 1104 42-43 0.8618755813697803 1104 44-45 -3.22354746867936 1104 46-47 0.5187422423848318 1104 48-49 0.8244640722511747 1104 50-51 1.0778706566972787 1104 52-53 0.37624278878914197 1104 54-55 -0.20045074273500063 1104 56-57 1.0633907448504587 1104 58-59 0.911820207433145 1104 60-61 0.31596542430948915 1104 62-63 0.5441064695191358 1104 64-65 -0.0648534139316368 1104 66-67 0.2827052751431225 1104 68-69 -3.0717302592073636 1104 70-71 -0.7006199887628384 1104 72-73 1.085782265208472 1104 74-75 -1.0801914501401555 1104 76 0.3408146520372952 1105 1 -1.6559912307598132 1105 2 -0.2667388880316359 1105 3 -1.0929886626924947 1105 4 -0.16946231193353611 1105 5 -0.146941026044086 1105 6 -0.46544492249599045 1105 7 0.928626169661257 1105 8 0.028552581647417696 1105 9 0.05829879159695395 1105 10-11 0.8691117470889083 1105 12-13 -1.6477142249069345 1105 14-15 -0.6930587034157938 1105 16-17 0.5535671555174062 1105 18-19 0.031425239193040966 1105 20-21 -0.9168966413769439 1105 22-23 -0.1007972584080008 1105 24-25 -0.17011920528062063 1105 26-27 -0.12148445792595908 1105 28-29 -1.213386571618976 1105 30-31 -0.2256122131824725 1105 32-33 0.5764326810995328 1105 34-35 0.7216474271157765 1105 36-37 0.31866150003490645 1105 38-39 -0.22873544399448775 1105 40-41 0.049371925525910854 1105 42-43 0.48784047882300996 1105 44-45 1.0732042779963251 1105 46-47 0.3152404393647714 1105 48-49 -0.26322890723946557 1105 50-51 -0.07921746590807999 1105 52-53 -1.0294085522070304 1105 54-55 -1.4545216239610568 1105 56-57 -0.593500172435995 1105 58-59 -0.32007916150983107 1105 60-61 -0.18349930411061166 1105 62-63 0.36281843706477446 1105 64-65 -0.2903929113397865 1105 66-67 -0.31809481500788195 1105 68-69 0.7015329253768172 1105 70-71 1.0989841919899277 1105 72-73 0.6699185059116459 1105 74-75 -0.7880035979878031 1105 76 -0.9354277414718339 1106 1 0.37492804071446884 1106 2 0.9566182372452552 1106 3 0.032442053527045545 1106 4 -0.11131562229533642 1106 5 0.637039407133372 1106 6 0.011665586979766829 1106 7 1.291443053737062 1106 8 1.4830658723193295 1106 9 1.3193128206395315 1106 10-11 0.6125817815462042 1106 12-13 0.920777345361337 1106 14-15 0.7174446192521842 1106 16-17 0.7439129591108085 1106 18-19 0.3122983625009397 1106 20-21 -0.11508763325486626 1106 22-23 0.48582284988636815 1106 24-25 1.083349598558904 1106 26-27 0.9029971443383786 1106 28-29 -0.5561982255692612 1106 30-31 -0.10781901849873776 1106 32-33 1.2831149355912927 1106 34-35 0.8921341364438646 1106 36-37 0.5309276826585858 1106 38-39 0.8584461336574947 1106 40-41 1.204929703030217 1106 42-43 1.2341563168737153 1106 44-45 1.8813355849123923 1106 46-47 1.0342442542798551 1106 48-49 1.0533552054851185 1106 50-51 0.8894017809538397 1106 52-53 0.17195128042895647 1106 54-55 0.7648821417293163 1106 56-57 1.2071089833602144 1106 58-59 -0.1649444088639953 1106 60-61 1.049056688997922 1106 62-63 0.9128122839759243 1106 64-65 0.8878389985789923 1106 66-67 1.1523088276207218 1106 68-69 1.2958213206512426 1106 70-71 0.9124148466785797 1106 72-73 0.6557817842773872 1106 74-75 -2.0025771889304522 1106 76 -0.9090771384691259 1107 1 -0.3393900415476665 1107 2 -0.40424154252303524 1107 3 0.27394168345318093 1107 4 0.29618856935761073 1107 5 0.04703593297056585 1107 6 0.36180077722794124 1107 7 0.2907784312879258 1107 8 1.4230232760623664 1107 9 -0.9420197473709777 1107 10-11 -0.28879731003574705 1107 12-13 0.5202818995356253 1107 14-15 -0.1703097121225241 1107 16-17 0.826900134918553 1107 18-19 1.0540547783733274 1107 20-21 1.1486692961894178 1107 22-23 -0.11835671901534539 1107 24-25 -0.03850288546750136 1107 26-27 0.4545733568967236 1107 28-29 -0.7863917213323006 1107 30-31 0.1167176339188245 1107 32-33 1.3200619017408641 1107 34-35 0.7915269132062477 1107 36-37 -0.03049702619146899 1107 38-39 0.993266307969833 1107 40-41 0.92691360104088 1107 42-43 0.6450062783558295 1107 44-45 0.7820198439338881 1107 46-47 0.4220439008215564 1107 48-49 0.3739751169694898 1107 50-51 0.7655478770522066 1107 52-53 0.8979380181900822 1107 54-55 0.663935054739305 1107 56-57 1.0779003372263531 1107 58-59 1.1393469374496092 1107 60-61 0.2791520018991491 1107 62-63 0.20755723511106794 1107 64-65 0.43857688054808364 1107 66-67 1.0488089862238041 1107 68-69 1.3341579519217248 1107 70-71 0.2807969441666103 1107 72-73 0.8034261508468816 1107 74-75 -0.06342035313751637 1107 76 -0.8817671978319979 1108 1 -1.264433496555018 1108 2 -0.3806822431514618 1108 3 -0.3428252639996856 1108 4 -0.43907015507079095 1108 5 -0.7154323114471346 1108 6 0.1491520273950755 1108 7 0.8103799822974196 1108 8 0.8896364596430573 1108 9 0.05176284388453922 1108 10-11 0.35798701942006517 1108 12-13 0.4500118099514552 1108 14-15 0.9598280068238552 1108 16-17 -0.2945265263998067 1108 18-19 0.12213874681830816 1108 20-21 0.31175259609582895 1108 22-23 -1.0272950797587654 1108 24-25 -1.030426939485416 1108 26-27 -0.6782518871198562 1108 28-29 0.3217577638929967 1108 30-31 0.8172390940070713 1108 32-33 0.7253433564807992 1108 34-35 -0.07554756199097312 1108 36-37 0.495649517464102 1108 38-39 1.168309762977188 1108 40-41 0.7799955638701377 1108 42-43 0.855936884051772 1108 44-45 0.06010514945602097 1108 46-47 0.8284485003669531 1108 48-49 0.30083699689996024 1108 50-51 0.5411529044622867 1108 52-53 0.16225811445963245 1108 54-55 0.45983022789079087 1108 56-57 0.8335695443396034 1108 58-59 -1.0437992050828129 1108 60-61 0.7973830488305609 1108 62-63 -0.36087437339273265 1108 64-65 0.2556446703595512 1108 66-67 -0.11755015269850588 1108 68-69 1.5434038404094892 1108 70-71 2.0565958477633473 1108 72-73 0.6884642575456397 1108 74-75 1.3205748333847467 1108 76 0.9632923021011521 1109 1 -0.2717598009616147 1109 2 -0.8734370417456745 1109 3 0.0984561634131289 1109 4 -0.33444728402455937 1109 5 0.5993825033676785 1109 6 0.5968135575383613 1109 7 -0.9329560544692725 1109 8 0.6581414609947771 1109 9 0.7727685210945907 1109 10-11 1.131898887492511 1109 12-13 -0.1527727264097294 1109 14-15 0.20008212955973192 1109 16-17 -0.6800631579218361 1109 18-19 0.42013820613163944 1109 20-21 1.1442884165878482 1109 22-23 1.348671397667566 1109 24-25 0.9914979050671704 1109 26-27 0.8158411109877406 1109 28-29 0.12424492258454478 1109 30-31 0.6390963527256446 1109 32-33 1.0026885849209108 1109 34-35 0.25902935068813093 1109 36-37 0.09498717629081455 1109 38-39 0.6008185491356102 1109 40-41 0.25094717241299236 1109 42-43 0.4371615393640198 1109 44-45 -0.019727237675638776 1109 46-47 -0.6685777229366465 1109 48-49 0.13714410692969636 1109 50-51 1.5339212202233057 1109 52-53 1.241576849252823 1109 54-55 0.701044069469603 1109 56-57 0.5604822234420652 1109 58-59 0.2762873047847165 1109 60-61 0.8882589612393161 1109 62-63 0.03397558605576023 1109 64-65 0.6569423183725362 1109 66-67 0.2744341673880015 1109 68-69 1.8482294689577472 1109 70-71 0.21922088155626085 1109 72-73 0.5476288966372849 1109 74-75 2.0575578017545766 1109 76 -0.16774311287587196 1110 1 0.00900648038690477 1110 2 1.1117230025611775 1110 3 1.0201665215601956 1110 4 0.5077521536192222 1110 5 -0.058205045270455 1110 6 -0.14809541459944597 1110 7 0.9638458995765689 1110 8 1.8970150444535463 1110 9 -1.2791926252032297 1110 10-11 -0.7214624689788423 1110 12-13 0.48028274236245494 1110 14-15 -0.01564396320063821 1110 16-17 0.23784845558607515 1110 18-19 1.0924947058902177 1110 20-21 -0.025788836387242497 1110 22-23 0.6991712698593417 1110 24-25 0.5774560407711036 1110 26-27 -0.6942628397066883 1110 28-29 0.5927117918509239 1110 30-31 0.09354691934213122 1110 32-33 0.8664876135783643 1110 34-35 1.0132044195625625 1110 36-37 0.6305320653816722 1110 38-39 0.49117328798079285 1110 40-41 0.6787994260981236 1110 42-43 0.7578607923959098 1110 44-45 1.7851663974744625 1110 46-47 0.8375996748877768 1110 48-49 0.9701697537813629 1110 50-51 0.4110549076327743 1110 52-53 0.08683658397974625 1110 54-55 -0.12353913368868064 1110 56-57 -0.038767968270278175 1110 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0.5088972881902762 2116 52-53 1.1939521035306768 2116 54-55 0.9010111629241919 2116 56-57 0.0020880628581210203 2116 58-59 -0.18549622997655746 2116 60-61 -0.7522071151038645 2116 62-63 0.6371675209606309 2116 64-65 0.9515007723631967 2116 66-67 -0.02277176775618983 2116 68-69 0.3583856254732467 2116 70-71 -2.727769665290694 2116 72-73 0.7236190845037456 2116 74-75 -1.312895111970441 2116 76 -1.1478795254641199 2117 1 0.08371465159313018 2117 2 0.054389563348916425 2117 3 0.8104627178597248 2117 4 -0.3315871618735109 2117 5 -0.8128623945915052 2117 6 -1.1299107088105131 2117 7 -2.6611997607108933 2117 8 -2.2932811518459815 2117 9 0.8161619368550603 2117 10-11 -0.13823369108636285 2117 12-13 0.7409037222719306 2117 14-15 -0.15725438168710326 2117 16-17 0.3737652347491007 2117 18-19 0.4218364036588227 2117 20-21 -0.4865845165270031 2117 22-23 0.3427351545571824 2117 24-25 0.7255065450194991 2117 26-27 0.9023225648831641 2117 28-29 0.8155975220184715 2117 30-31 0.2721143774537893 2117 32-33 1.140838443404455 2117 34-35 0.013379119792851668 2117 36-37 -1.206353505967492 2117 38-39 0.01301875768618288 2117 40-41 -1.6695131284956943 2117 42-43 -1.3704803495083198 2117 44-45 0.4701580595089325 2117 46-47 -0.29846004082730815 2117 48-49 0.38481280944720453 2117 50-51 1.047313146336819 2117 52-53 1.1762036926909332 2117 54-55 -1.5756950933488127 2117 56-57 -0.8326058146928972 2117 58-59 -0.6637916465038245 2117 60-61 1.3024850896468934 2117 62-63 0.14502131821724618 2117 64-65 -0.08505827748624739 2117 66-67 -1.5814201451399335 2117 68-69 0.09997980412831708 2117 70-71 0.8081453564557108 2117 72-73 1.5519563210377 2117 74-75 -0.009357139404365 2117 76 -0.6932458687227339 2118 1 0.14774627214648817 2118 2 1.0009895472159798 2118 3 0.5440434228690023 2118 4 0.5596123218725566 2118 5 0.2396987089012761 2118 6 0.7536901623679952 2118 7 0.9136005942136833 2118 8 -3.986545651321663 2118 9 -1.2154747494400127 2118 10-11 0.0695665831735397 2118 12-13 1.1474133617008277 2118 14-15 -0.47110450671734583 2118 16-17 0.049178641218404096 2118 18-19 -0.6516571834993599 2118 20-21 -3.2529231468407893 2118 22-23 -0.23208939977047294 2118 24-25 -0.519681807001156 2118 26-27 0.4008141354244259 2118 28-29 -0.4302038815469089 2118 30-31 0.2710818695889827 2118 32-33 0.15135711727716483 2118 34-35 0.8202436838002711 2118 36-37 -0.07373271061378261 2118 38-39 -0.0037514286492239535 2118 40-41 -1.374344942644285 2118 42-43 0.00826450811852908 2118 44-45 0.3396103463525151 2118 46-47 -0.23662249948665703 2118 48-49 -1.507975768434541 2118 50-51 -0.508071220848791 2118 52-53 -2.1919579593216554 2118 54-55 0.1218123681338028 2118 56-57 0.8854872723442853 2118 58-59 0.9918105153095169 2118 60-61 -1.1826564768611334 2118 62-63 0.27676286859232846 2118 64-65 -0.16949806130491396 2118 66-67 -2.049726993571003 2118 68-69 1.626034494779283 2118 70-71 -0.6123838038250042 2118 72-73 -1.9859318777200627 2118 74-75 0.40568682284456514 2118 76 -0.06302888074177204 2119 1 0.4088247837517187 2119 2 0.0891823350831622 2119 3 0.05345471642126398 2119 4 0.007332194415258897 2119 5 0.7403516663632743 2119 6 0.7489888686416108 2119 7 -1.854834571463389 2119 8 0.938616717433888 2119 9 0.11466054334611187 2119 10-11 0.47117024670020413 2119 12-13 0.4368041088582899 2119 14-15 -0.3658042333458269 2119 16-17 0.18167676940701227 2119 18-19 0.053824176741628094 2119 20-21 -0.29115162971589115 2119 22-23 1.0413865975132168 2119 24-25 -0.058310770452941085 2119 26-27 0.38903478280997916 2119 28-29 0.7778109536466289 2119 30-31 -0.15260916835219973 2119 32-33 -0.028711138542860226 2119 34-35 0.6127947450737992 2119 36-37 -0.051593099602300185 2119 38-39 -0.23071944243967835 2119 40-41 -0.6211628632794799 2119 42-43 -0.6324870023897304 2119 44-45 -1.7993825320580967 2119 46-47 -0.7563940514359544 2119 48-49 -0.5960466709088195 2119 50-51 0.4780512565588779 2119 52-53 -1.4143204464801613 2119 54-55 -1.1232991570007016 2119 56-57 0.3809426884087799 2119 58-59 1.2422588794334217 2119 60-61 0.609938114909859 2119 62-63 -0.13028210711765098 2119 64-65 0.20378466057002953 2119 66-67 0.575336125650324 2119 68-69 -0.3453137317882522 2119 70-71 -0.08057171627646653 2119 72-73 -1.540806422826364 2119 74-75 0.13760860724671176 2119 76 0.3166615303602427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 67.0 14 244.0 15 509.0 16 719.0 17 743.0 18 814.0 19 798.0 20 731.0 21 738.0 22 762.0 23 808.0 24 913.0 25 1066.0 26 1353.0 27 1649.0 28 1935.0 29 2349.0 30 2915.0 31 3704.0 32 4535.0 33 5972.0 34 8335.0 35 12122.0 36 19697.0 37 21497.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.170441886372075 21.052464060262054 10.901278446766751 30.875815606599122 2 18.05280667031625 18.993978186718323 34.948625089484985 28.004590053480438 3 18.671832231439762 23.323998820903693 28.482545163599614 29.52162378405693 4 14.492356929296331 17.279024718911863 36.24668379163683 31.98193456015497 5 13.473280835473956 35.08232618857119 34.08114709226429 17.36324588369057 6 29.391080978649935 36.693055964963996 17.143218090706196 16.772644965679877 7 27.639280751252787 30.910220238345897 21.567987535267612 19.8825114751337 8 24.049353602560323 33.41264159683329 20.172021729060514 22.36598307154588 9 23.639828188823852 16.642102160272877 21.683791636838336 38.03427801406494 10-11 24.696803806796648 25.100539015454583 27.000252663494333 23.202404514254432 12-13 24.198319787762664 23.337158377900366 26.635996125826424 25.828525708510547 14-15 21.92171642733819 25.01894976207521 23.91617888575399 29.143154924832608 16-17 22.50021055291195 27.56242893839222 25.875373731418705 24.06198677727713 18-19 21.97488103760475 26.65336674106203 26.763907019834082 24.60784520149914 20-21 21.523245041478926 26.94656167094791 26.591780014317596 24.93841327325557 22-23 22.75866425232661 26.841285214974526 26.500189497620752 23.899861035078114 24-25 22.14016507348297 27.651387543689733 26.240683033646356 23.96776434918095 26-27 21.04055249084095 28.219880405946014 26.89866088348002 23.840906219733018 28-29 22.152798248199773 27.13184823346107 28.130395418368636 22.584958099970525 30-31 22.03699414662905 26.86181412388933 27.24765233503179 23.853539394449825 32-33 20.82947319661431 28.023013433275786 27.247125952751926 23.900387417357983 34-35 22.30386996252158 27.30344885669769 27.129216322061733 23.263464858718997 36-37 22.285972965006106 27.308712679496356 26.761275108434752 23.644039247062786 38-39 21.575883269465617 27.479260538173243 27.91405230134333 23.03080389101781 40-41 21.742746452183432 26.331747168063334 27.87983745315198 24.045668926601255 42-43 21.269002400303197 26.312271023708256 28.11249842085316 24.306228155135383 44-45 20.631027077104477 25.79852191855813 28.503074072514423 25.067376931822967 46-47 21.003179348970395 25.52269760390786 29.233166294689855 24.240956752431885 48-49 20.32993641302059 25.894323493493914 29.852718238093235 23.92302185539226 50-51 19.83145239398661 26.1285636080347 29.092095843685517 24.947888154293175 52-53 19.57931528193035 26.47808144186634 29.08420010948751 24.858403166715796 54-55 18.79658483176822 26.950772729186845 28.827851939192318 25.424790499852612 56-57 19.128732050364256 26.862340506169204 28.70046742746452 25.308460016002023 58-59 19.124520992125323 26.394913041647367 28.547816566303112 25.932749399924198 60-61 19.4161367751716 26.021181622941846 28.884174843138084 25.678506758748476 62-63 19.675643239145998 26.02223438750158 28.459384343285464 25.842738030066958 64-65 20.109908620036215 26.359645428896282 27.41083084179054 26.11961510927696 66-67 20.255190129279487 26.31753484650693 26.93971870130964 26.487556322903945 68-69 20.090958857961006 25.920116225207394 26.772329136311956 27.216595780519643 70-71 20.64260748726155 26.331220785783465 26.462289973470334 26.56388175348465 72-73 20.893165452478208 26.674422032256707 25.61744641428391 26.814966100981174 74-75 20.662610013896494 27.330820735250764 25.295826841285212 26.710742409567523 76 21.62273129237377 26.73600875900114 24.87682654651114 26.76443340211395 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 1.5 10 1.5 11 0.5 12 1.0 13 0.5 14 1.0 15 2.0 16 1.5 17 1.0 18 4.0 19 14.5 20 26.5 21 31.0 22 43.5 23 70.0 24 100.0 25 116.0 26 176.5 27 261.0 28 390.0 29 495.0 30 569.5 31 734.5 32 1027.0 33 1229.0 34 1456.5 35 1951.5 36 2403.5 37 2588.0 38 3048.0 39 3949.0 40 4838.0 41 5583.0 42 5880.0 43 5991.5 44 6469.5 45 6925.0 46 7014.0 47 6948.0 48 6644.0 49 6245.5 50 6085.0 51 5710.5 52 4889.0 53 4005.5 54 3569.0 55 3310.0 56 2710.5 57 2144.5 58 1919.0 59 1635.5 60 1210.5 61 926.5 62 784.0 63 714.0 64 539.5 65 381.5 66 283.5 67 239.0 68 203.0 69 135.0 70 84.5 71 66.0 72 50.5 73 39.0 74 31.5 75 20.0 76 19.0 77 14.5 78 10.5 79 10.0 80 10.5 81 7.0 82 3.0 83 3.0 84 2.5 85 3.0 86 4.5 87 5.0 88 3.0 89 0.5 90 0.5 91 2.5 92 4.0 93 2.0 94 0.5 95 2.0 96 3.0 97 2.5 98 2.5 99 7.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7716764222849202 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 94988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.57240914641849 #Duplication Level Percentage of deduplicated Percentage of total 1 75.12886837746804 42.502210805575444 2 10.646295848298193 12.04573209247484 3 5.38548858328526 9.140101907609383 4 2.715075274019763 6.143933970606814 5 1.5854997487764484 4.484777024466249 6 1.0811917300928597 3.66993725523224 7 0.7592534008225245 3.0066955825999075 8 0.5340826618530994 2.417147429148945 9 0.4689506299197945 2.387670021476397 >10 1.6934328302659247 14.144944624584157 >50 0.001860915198094423 0.0568492862256285 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007369351918137028 0.0 2 0.0 0.0 0.0 0.011580410157072472 0.0 3 0.0 0.0 0.0 0.011580410157072472 0.0 4 0.0 0.0 0.0 0.016844232955741778 0.0 5 0.0 0.0 0.0 0.0189497620752095 0.0 6 0.0 0.0 0.0 0.0189497620752095 0.0 7 0.0 0.0 0.0 0.02000252663494336 0.0 8 0.0 0.0 0.0 0.022108055754411084 0.0 9 0.0 0.0 0.0 0.03474123047121742 0.0 10 0.0 0.0 0.0 0.042110582389354445 0.0 11 0.0 0.0 0.0 0.04842716974775761 0.0 12 0.0 0.0 0.0 0.051585463426959195 0.0 13 0.0 0.0 0.0 0.05474375710616078 0.0 14 0.0 0.0 0.0 0.0568492862256285 0.0 15 0.0 0.0 0.0 0.060007579904830084 0.0 16 0.0 0.0 0.0 0.06527140270349939 0.0 17 0.0 0.0 0.0 0.07895734198003958 0.0 18 0.0 0.0 0.0 0.08316840021897502 0.0 19 0.0 0.0 0.0 0.08737945845791047 0.0 20 0.0 0.0 0.0 0.09264328125657978 0.0 21 0.0 0.0 0.0 0.09580157493578136 0.0 22 0.0 0.0 0.0 0.10632922053311997 0.0 23 0.0 0.0 0.0 0.10948751421232156 0.0 24 0.0 0.0 0.0 0.11054027877205541 0.0 25 0.0 0.0 0.0 0.11580410157072472 0.0 26 0.0 0.0 0.0 0.12001515980966017 0.0 27 0.0 0.0 0.0 0.15265086116140986 0.0 28 0.0 0.0 0.0 0.1810755042742241 0.0 29 0.0 0.0 0.0 0.20739461826757064 0.0 30 0.0 0.0 0.0 0.22529161578304627 0.0 31 0.0 0.0 0.0 0.26108561081399756 0.0 32 0.0 0.0 0.0 0.3158293679201583 0.0 33 0.0 0.0 0.0 0.35162336295110963 0.0 34 0.0 0.0 0.0 0.41478923653514127 0.0 35 0.0 0.0 0.0 0.49690487219438245 0.0 36 0.0 0.0 0.0 0.6285004421611151 0.0 37 0.0 0.0 0.0 0.9948625089484987 0.0 38 0.0 0.0 0.0 1.4770286773066073 0.0 39 0.0 0.0 0.0 1.9991998989346023 0.0 40 0.0 0.0 0.0 2.614014401819177 0.0 41 0.0 0.0 0.0 3.54465827262391 0.0 42 0.0 0.0 0.0 4.478460437107845 0.0 43 0.0 0.0 0.0 5.4175264243904495 0.0 44 0.0 0.0 0.0 6.387122583905335 0.0 45 0.0 0.0 0.0 7.270392049522045 0.0 46 0.0 0.0 0.0 8.254726912873204 0.0 47 0.0 0.0 0.0 9.11483555817577 0.0 48 0.0 0.0 0.0 9.910725565334568 0.0 49 0.0 0.0 0.0 10.5708089442877 0.0 50 0.0 0.0 0.0 11.269844611950983 0.0 51 0.0 0.0 0.0 12.0320461531983 0.0 52 0.0 0.0 0.0 12.927948793531815 0.0 53 0.0 0.0 0.0 13.897544953046701 0.0 54 0.0 0.0 0.0 14.859771760643449 0.0 55 0.0 0.0 0.0 15.728302522423885 0.0 56 0.0 0.0 0.0 16.474712595275193 0.0 57 0.0 0.0 0.0 17.13900703246726 0.0 58 0.0 0.0 0.0 17.810670821577464 0.0 59 0.0 0.0 0.0 18.451804438455383 0.0 60 0.0 0.0 0.0 19.07819935149703 0.0 61 0.0 0.0 0.0 19.685644502463468 0.0 62 0.0 0.0 0.0 20.4109992841201 0.0 63 0.0 0.0 0.0 21.157409356971407 0.0 64 0.0 0.0 0.0 21.906977723501917 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGAG 15 0.002219666 69.99157 19 CCGCGTG 15 0.002219666 69.99157 9 ACATCCA 15 0.002219666 69.99157 19 ATCCTTA 25 2.3812595E-4 55.993263 42 AAGACGA 25 2.3812595E-4 55.993263 29 CCTATGG 25 2.3812595E-4 55.993263 42 GCATGAC 25 2.3812595E-4 55.993263 17 CCCCTTG 20 0.006935811 52.49368 31 GACTTCC 20 0.006935811 52.49368 23 ACGCTTA 20 0.006935811 52.49368 59 CCCTTGT 20 0.006935811 52.49368 32 CGTCCTT 20 0.006935811 52.49368 4 CGCTTAG 20 0.006935811 52.49368 60 GCTCAAA 20 0.006935811 52.49368 52 CTATGGA 30 5.855519E-4 46.66105 43 GAGCTTT 30 5.855519E-4 46.66105 48 GAAGACC 30 5.855519E-4 46.66105 36 CTGACTT 30 5.855519E-4 46.66105 21 AATTCCC 30 5.855519E-4 46.66105 32 TTCGGTC 30 5.855519E-4 46.66105 33 >>END_MODULE