FastQCFastQC Report
Wed 25 May 2016
SRR1780676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94988
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT7660.8064176527561375No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT3890.409525413736472RNA PCR Primer, Index 30 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1080.113698572451257No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC980.10317092685391839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAG150.002218606470.05
CGAGGCC150.002218606470.08
CGGGCGA150.002218606470.04
AGACCGG150.002218606470.010
TGGGCCG150.002218606470.010
GCCCCCC200.006932509652.511
CGGAATC200.006932509652.520
CTGTAGC200.006932509652.51
CGTGCTT200.006932509652.565
AACCCGT200.006932509652.538
TCGGGCG200.006932509652.53
ATTGTTC200.006932509652.58
CTGCGTG200.006932509652.562
CGTTACA200.006932509652.514
AGCGGAA200.006932509652.518
CGTAGCG200.006932509652.515
GAGCGGT200.006932509652.56
TGTTCGT200.006932509652.510
GCGTGCT200.006932509652.564
TGGTCCA200.006932509652.521
Produced by FastQC (version 0.11.5)