##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94988 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7630648081863 34.0 34.0 34.0 31.0 34.0 2 33.29518465490378 34.0 34.0 34.0 32.0 34.0 3 33.42708552659283 34.0 34.0 34.0 34.0 34.0 4 33.48864067040047 34.0 34.0 34.0 34.0 34.0 5 33.513285888743845 34.0 34.0 34.0 34.0 34.0 6 37.227934054827976 38.0 37.0 38.0 36.0 38.0 7 36.88947024887354 38.0 37.0 38.0 35.0 38.0 8 36.8987345769992 38.0 37.0 38.0 35.0 38.0 9 36.685939276540196 38.0 37.0 38.0 34.0 38.0 10-11 36.42650124226218 38.0 37.0 38.0 34.0 38.0 12-13 36.30071693266518 38.0 37.0 38.0 34.0 38.0 14-15 36.583210510801365 38.0 37.0 38.0 34.0 38.0 16-17 36.71404282646229 38.0 37.0 38.0 34.0 38.0 18-19 36.73086074030404 38.0 37.0 38.0 34.0 38.0 20-21 36.46403756263949 38.0 37.0 38.0 34.0 38.0 22-23 36.536494083463175 38.0 37.0 38.0 34.0 38.0 24-25 35.896744851981296 38.0 36.5 38.0 32.5 38.0 26-27 36.10193919231903 38.0 37.0 38.0 33.5 38.0 28-29 35.96629574262012 38.0 37.0 38.0 32.5 38.0 30-31 35.71405335410789 38.0 36.5 38.0 31.5 38.0 32-33 36.21012127847728 38.0 37.0 38.0 34.0 38.0 34-35 36.25303196193204 38.0 37.0 38.0 33.5 38.0 36-37 36.37393670779467 38.0 37.0 38.0 34.0 38.0 38-39 36.039810291826335 38.0 37.0 38.0 32.5 38.0 40-41 35.56119720385733 38.0 36.0 38.0 31.0 38.0 42-43 35.959700172653385 38.0 36.5 38.0 32.5 38.0 44-45 36.12991114667116 38.0 37.0 38.0 34.0 38.0 46-47 36.42874889459721 38.0 37.0 38.0 34.0 38.0 48-49 36.23757737819514 38.0 37.0 38.0 34.0 38.0 50-51 36.38153240409315 38.0 37.0 38.0 34.0 38.0 52-53 36.30844948835642 38.0 37.0 38.0 34.0 38.0 54-55 36.47764980839685 38.0 37.0 38.0 34.0 38.0 56-57 36.52426622310187 38.0 37.0 38.0 34.0 38.0 58-59 36.56627152903525 38.0 37.0 38.0 34.0 38.0 60-61 36.25958015749358 38.0 37.0 38.0 33.5 38.0 62-63 36.535567650650606 38.0 37.0 38.0 34.0 38.0 64-65 36.53413589084937 38.0 37.0 38.0 34.0 38.0 66-67 35.993536025603234 38.0 37.0 38.0 33.0 38.0 68-69 35.47478102497158 38.0 36.0 38.0 31.0 38.0 70-71 35.49799974733651 38.0 36.0 38.0 31.0 38.0 72-73 35.29249484145366 38.0 36.0 38.0 29.0 38.0 74-75 35.86815703036173 38.0 37.0 38.0 32.5 38.0 76 35.31430285930855 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.119297016250556 1101 2 0.3915692177828092 1101 3 0.20619342721240486 1101 4 0.2317026901185173 1101 5 0.1559345083958803 1101 6 -1.8053562722076393 1101 7 -0.07868998747387224 1101 8 0.3660875094674694 1101 9 0.051366054202446776 1101 10-11 -0.11978648552076265 1101 12-13 0.23656589201781486 1101 14-15 0.39530312211449115 1101 16-17 -0.344320211727144 1101 18-19 -0.7948754669582243 1101 20-21 -0.26447379236482504 1101 22-23 0.25711503237079825 1101 24-25 -1.6638141236432062 1101 26-27 -0.543976789248731 1101 28-29 0.23757834726485783 1101 30-31 1.0244028006620525 1101 32-33 -0.12377384984318951 1101 34-35 0.3141798941719216 1101 36-37 0.02700298462612949 1101 38-39 0.3249240034675154 1101 40-41 1.0319711854470484 1101 42-43 0.3196846414963659 1101 44-45 0.22136581878763906 1101 46-47 0.4012613182419713 1101 48-49 -1.489007020405019 1101 50-51 -2.0169488724923355 1101 52-53 -2.1878337262579493 1101 54-55 -1.1333736989677945 1101 56-57 -1.1418498336076937 1101 58-59 -1.901717019325531 1101 60-61 -2.252569284358408 1101 62-63 -0.8466094285845784 1101 64-65 -1.212822770782374 1101 66-67 -1.3892414349777624 1101 68-69 -0.9388110367198053 1101 70-71 -0.7963880471152223 1101 72-73 -1.5282499110333632 1101 74-75 -0.6432607834093673 1101 76 -0.6704592228769144 1102 1 -0.7706105338264848 1102 2 0.3762379388922099 1102 3 0.32953703275940427 1102 4 0.4433359720907859 1102 5 0.10608859160080897 1102 6 0.23170073857818352 1102 7 -0.14594730642610898 1102 8 0.2959026096215496 1102 9 0.4303336967294058 1102 10-11 0.24600704298463683 1102 12-13 0.2633378488745137 1102 14-15 -0.2481098208747241 1102 16-17 -0.06562221480880481 1102 18-19 -0.40751028976253423 1102 20-21 0.42258321842100344 1102 22-23 0.22760809862657538 1102 24-25 -4.593821827803453 1102 26-27 -1.1288766351655255 1102 28-29 -0.007799156587218192 1102 30-31 1.014541475546494 1102 32-33 -0.039605899149812274 1102 34-35 0.6867916044954967 1102 36-37 -2.7063945500425888 1102 38-39 -0.6404072754230867 1102 40-41 0.8102069327505887 1102 42-43 0.4977277847937458 1102 44-45 0.4536847710218481 1102 46-47 0.845059469243516 1102 48-49 -3.881688068170817 1102 50-51 -1.0065097353582786 1102 52-53 -0.23255637648907168 1102 54-55 0.514392094560705 1102 56-57 0.7514860677790622 1102 58-59 -0.07710222733786054 1102 60-61 -5.953686387594157 1102 62-63 -2.5880578107109287 1102 64-65 -0.27233740868684464 1102 66-67 0.6882624171023579 1102 68-69 -1.0674705128369055 1102 70-71 -1.041033655743881 1102 72-73 -5.984528801634291 1102 74-75 -2.0342854367221506 1102 76 -0.8981941997644327 1103 1 0.749676096629635 1103 2 0.5503266765402657 1103 3 0.22121624223522218 1103 4 0.39021444863028165 1103 5 0.22451997698134818 1103 6 0.39446822761686207 1103 7 0.7769962182123322 1103 8 0.6607347541147135 1103 9 0.2977507505871415 1103 10-11 0.18348659275232393 1103 12-13 -0.2593537289018073 1103 14-15 -0.0985368217254603 1103 16-17 0.037831420424488726 1103 18-19 0.679861282778532 1103 20-21 -0.05798906088009659 1103 22-23 0.5537893040450683 1103 24-25 -2.4872433970724828 1103 26-27 -0.46130743157937104 1103 28-29 0.09437020405671603 1103 30-31 0.9412069424662022 1103 32-33 0.9592816082122724 1103 34-35 0.562012467004493 1103 36-37 -0.7646695320463905 1103 38-39 0.2952644738079897 1103 40-41 0.008655987131852783 1103 42-43 -0.18714231533058978 1103 44-45 1.0309130283348509 1103 46-47 0.9278583698390221 1103 48-49 -2.0737648301628298 1103 50-51 -0.43283163837509875 1103 52-53 0.2520486886244697 1103 54-55 -0.15073944133353478 1103 56-57 0.0422147504568926 1103 58-59 0.0495992319907117 1103 60-61 -3.744505051294176 1103 62-63 -1.2453282773034289 1103 64-65 0.17103998808038767 1103 66-67 -0.22965035038667736 1103 68-69 0.46317211526334745 1103 70-71 0.49747822175104517 1103 72-73 -4.465289223072681 1103 74-75 -2.039856080004661 1103 76 -2.4880443404620323 1104 1 0.23669513537281262 1104 2 0.34862250931885796 1104 3 0.4577434097968691 1104 4 -0.1718500789514934 1104 5 -0.3405475536034217 1104 6 0.018294023856448405 1104 7 0.4002894621952322 1104 8 0.48818782294708996 1104 9 0.8458801607855122 1104 10-11 -0.4738530128631524 1104 12-13 0.12558539741662145 1104 14-15 0.5492390329469501 1104 16-17 0.1724857792167782 1104 18-19 0.3186651112375287 1104 20-21 -0.11026794074518165 1104 22-23 0.24885136117608653 1104 24-25 0.5214697495717004 1104 26-27 0.9154363072333354 1104 28-29 1.3220436903508883 1104 30-31 1.0508831211078942 1104 32-33 0.36973886349711194 1104 34-35 0.03254457546763945 1104 36-37 1.0792408808295377 1104 38-39 0.8405979846587428 1104 40-41 1.4222271938064992 1104 42-43 0.6505623844430772 1104 44-45 0.8150962263111552 1104 46-47 1.0901240838288757 1104 48-49 0.2163134254326664 1104 50-51 0.567464223989731 1104 52-53 0.485720784365526 1104 54-55 0.3603480007883917 1104 56-57 0.31586524824532347 1104 58-59 -0.5275616727563985 1104 60-61 0.5811210674353902 1104 62-63 0.3482529886916339 1104 64-65 0.6084503048010461 1104 66-67 0.15144996174811354 1104 68-69 1.3085766330816568 1104 70-71 1.1833315627768002 1104 72-73 0.8559803223347942 1104 74-75 -0.30874319371935854 1104 76 -0.7469830821593959 1105 1 0.9306606526141934 1105 2 0.3566093247346771 1105 3 0.3196292560894136 1105 4 -0.07317698902586756 1105 5 -0.11973394080197863 1105 6 -0.08597124546967194 1105 7 0.5873527932747891 1105 8 -0.7684044691421619 1105 9 0.4131976050045836 1105 10-11 0.27382982463966954 1105 12-13 -0.8850918619928834 1105 14-15 0.12555044831769635 1105 16-17 0.17456347811242523 1105 18-19 0.5529520731487381 1105 20-21 -0.07891229503862718 1105 22-23 0.0994612890552915 1105 24-25 0.37592518116962736 1105 26-27 0.2221328364197177 1105 28-29 0.85171604779962 1105 30-31 1.0848306081486854 1105 32-33 0.4669681287664318 1105 34-35 -1.0059738491435866 1105 36-37 -0.047963592913980335 1105 38-39 -0.15084471311001835 1105 40-41 -0.009272130060971051 1105 42-43 0.23993865219155452 1105 44-45 0.9329968348304192 1105 46-47 0.6972840455146994 1105 48-49 0.11049164269658007 1105 50-51 0.6278372237643524 1105 52-53 0.25177188871531797 1105 54-55 0.5719070496953123 1105 56-57 0.2940458883174415 1105 58-59 0.4084099860220505 1105 60-61 0.7552181487966649 1105 62-63 -0.8491495355364478 1105 64-65 0.2696304377737775 1105 66-67 0.2796756773216984 1105 68-69 0.05316757290586338 1105 70-71 0.6153098138473396 1105 72-73 0.6129599820147646 1105 74-75 -0.17925677271417584 1105 76 0.5586851621498212 1106 1 -0.99141047709292 1106 2 -0.05449392409623499 1106 3 0.20307437053686073 1106 4 -0.13703066857299717 1106 5 0.060613093409195073 1106 6 0.19704007621381692 1106 7 -0.707503224446107 1106 8 0.19356944176111313 1106 9 -0.9809846494871692 1106 10-11 0.3180320766701854 1106 12-13 1.1122315443571082 1106 14-15 -1.0888923894868796 1106 16-17 -0.06027684923190435 1106 18-19 0.09710817701287056 1106 20-21 0.07986816275118969 1106 22-23 0.5491367136184166 1106 24-25 -0.7455752495465902 1106 26-27 0.48303918640741017 1106 28-29 0.3130823411788981 1106 30-31 0.3355382133665046 1106 32-33 -0.0063813051493895045 1106 34-35 0.7478333745827257 1106 36-37 1.0361414403127043 1106 38-39 0.11400730510312229 1106 40-41 0.7579411359985855 1106 42-43 0.528718494672475 1106 44-45 0.8375316613133208 1106 46-47 0.6997683551381257 1106 48-49 0.9383512907398952 1106 50-51 -0.29171113951401395 1106 52-53 0.10399776860702303 1106 54-55 0.08801275976176015 1106 56-57 0.18979718046610117 1106 58-59 0.37968275245080463 1106 60-61 0.7015862881025967 1106 62-63 0.46911898526099094 1106 64-65 -1.576411587823067 1106 66-67 0.04787343759736018 1106 68-69 0.9526876319755146 1106 70-71 -0.007028975224770306 1106 72-73 -0.0030026687359168136 1106 74-75 -0.8315072649612816 1106 76 -0.2502614290389502 1107 1 0.8182960316755654 1107 2 -0.24318627942756166 1107 3 0.0031740639042325824 1107 4 0.18700298549102 1107 5 -0.029428143392763673 1107 6 0.3452508823706353 1107 7 0.6077392872225502 1107 8 0.17224110316618635 1107 9 -0.44392439542013307 1107 10-11 0.48460307798432467 1107 12-13 -0.00945478405702005 1107 14-15 0.0752013826986726 1107 16-17 0.5894671200746231 1107 18-19 0.5627139652701985 1107 20-21 0.7405557448849223 1107 22-23 -0.03754364829871548 1107 24-25 0.287006355516624 1107 26-27 -0.21592460677416625 1107 28-29 0.6207421149051626 1107 30-31 0.6348146812002895 1107 32-33 0.9436285131367939 1107 34-35 0.10814937234717092 1107 36-37 0.962209745374409 1107 38-39 0.36020460734017945 1107 40-41 0.5717562199071722 1107 42-43 0.6874726296814657 1107 44-45 0.579816754133887 1107 46-47 0.6973968613991772 1107 48-49 0.7778374167462729 1107 50-51 0.7140816493324138 1107 52-53 0.33109472795931083 1107 54-55 0.5949082176715237 1107 56-57 0.6101029067927044 1107 58-59 0.5324477328897572 1107 60-61 0.9645048869576343 1107 62-63 0.5121532888423985 1107 64-65 0.8370213381772587 1107 66-67 0.14834471533401938 1107 68-69 0.8850320876648325 1107 70-71 0.3442995144207188 1107 72-73 1.381866597007324 1107 74-75 -0.3796244197730019 1107 76 0.8756845250718897 1108 1 -0.6610060140524752 1108 2 0.08348840970199944 1108 3 -0.11569209467311481 1108 4 0.37042322883905854 1108 5 -0.03901498655361735 1108 6 -0.5073764742278399 1108 7 -0.19805025683414357 1108 8 -0.5455286521486968 1108 9 -0.5935834407470537 1108 10-11 0.2784458377115655 1108 12-13 0.7622392926912127 1108 14-15 0.3010270278384013 1108 16-17 -0.2805154979224298 1108 18-19 0.2659831189003654 1108 20-21 0.8807282278371886 1108 22-23 -0.27764927739351464 1108 24-25 0.8738963140672311 1108 26-27 -0.7073606276325677 1108 28-29 -0.9238694641829497 1108 30-31 0.05950381076306854 1108 32-33 0.4795089784396396 1108 34-35 0.14608225107427586 1108 36-37 0.8026763852995273 1108 38-39 0.9353617139052304 1108 40-41 0.7685873470632103 1108 42-43 -0.3426049207931925 1108 44-45 0.5816351790570025 1108 46-47 0.5850155269961803 1108 48-49 0.7425586361606307 1108 50-51 0.4002228446793836 1108 52-53 -0.10374281716703848 1108 54-55 -0.47049491108900554 1108 56-57 -0.013061954819811206 1108 58-59 0.06713819952967981 1108 60-61 0.8515552947661646 1108 62-63 0.6244343424591889 1108 64-65 0.3419752090156081 1108 66-67 0.5576440869077572 1108 68-69 1.2014246535158861 1108 70-71 -0.7560681817953565 1108 72-73 -0.44516910295255485 1108 74-75 -0.8962910864396676 1108 76 0.4908202384025486 1109 1 -1.245434237896756 1109 2 0.439611686263234 1109 3 0.3544349666948179 1109 4 0.16853082548680476 1109 5 0.06714884150802192 1109 6 0.07380762958400311 1109 7 0.09486674776169224 1109 8 0.47912666009785454 1109 9 0.8249891464386749 1109 10-11 0.6470184248972899 1109 12-13 0.7306767082089252 1109 14-15 0.6661392203228473 1109 16-17 0.6905172136212272 1109 18-19 0.572957976970109 1109 20-21 0.798449233514404 1109 22-23 0.44916878154133855 1109 24-25 0.33777844437271654 1109 26-27 0.17637822070481946 1109 28-29 -0.785480710465734 1109 30-31 0.5951891311199162 1109 32-33 0.6060702112052496 1109 34-35 -0.1631094223509706 1109 36-37 0.7196539468026373 1109 38-39 1.0963241361815221 1109 40-41 0.4796984581743189 1109 42-43 0.9325910781254265 1109 44-45 0.6825137948991227 1109 46-47 0.7111982790913416 1109 48-49 0.6354621235896687 1109 50-51 0.07840613746121505 1109 52-53 -0.02564062738601791 1109 54-55 -0.5395946677800083 1109 56-57 0.2517798943286067 1109 58-59 0.1771814544634509 1109 60-61 0.7934990633522787 1109 62-63 0.46337730001528854 1109 64-65 0.8972469040683251 1109 66-67 0.4875170255398231 1109 68-69 0.3632792088010959 1109 70-71 0.528711488385774 1109 72-73 0.11549594165408905 1109 74-75 -0.09130325188979072 1109 76 0.6876031527037085 1110 1 -0.8694366236503974 1110 2 -0.4938570291220401 1110 3 0.07050188140412672 1110 4 -0.054537140090182845 1110 5 0.2808726053184145 1110 6 -0.06396964327237242 1110 7 0.4008112505876795 1110 8 0.6453057620320237 1110 9 0.8617127114362617 1110 10-11 -0.6975809673035727 1110 12-13 0.5248131505958114 1110 14-15 0.8136441196383686 1110 16-17 0.47306570974438245 1110 18-19 -0.20040252744637144 1110 20-21 0.7733136147805553 1110 22-23 -0.7104565983331312 1110 24-25 -1.2217730907461402 1110 26-27 1.0977193550738775 1110 28-29 0.07716094365679993 1110 30-31 -0.3978027175551304 1110 32-33 0.521035134946338 1110 34-35 0.7281257731956146 1110 36-37 1.0670012159667692 1110 38-39 0.8406650717377744 1110 40-41 -0.8004571838490264 1110 42-43 0.15472178334284337 1110 44-45 0.7617619984834363 1110 46-47 0.45178307275205754 1110 48-49 0.6880623110417403 1110 50-51 -0.5245717943175947 1110 52-53 0.17816379761895007 1110 54-55 -0.751493614072146 1110 56-57 0.28765140445386095 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0.6586721351477394 2116 38-39 -3.7989582618081883 2116 40-41 -0.6780884270715788 2116 42-43 -1.2601216178180863 2116 44-45 -0.2930157013315835 2116 46-47 -0.7058921839934982 2116 48-49 0.08472621357410759 2116 50-51 -1.092992091568334 2116 52-53 0.104863740210007 2116 54-55 -0.1935823427829959 2116 56-57 -0.16647725536626012 2116 58-59 -2.1633414604581773 2116 60-61 -1.5638211472496195 2116 62-63 0.16374824652719866 2116 64-65 -1.2817497454470512 2116 66-67 -0.30343666597627816 2116 68-69 -1.0025219160044543 2116 70-71 0.833306438726801 2116 72-73 0.5034192394882453 2116 74-75 -0.062001468953326366 2116 76 -0.8164353778694604 2117 1 0.7709667750631723 2117 2 0.5442593847586892 2117 3 0.2533177314795694 2117 4 -0.12043089058649059 2117 5 0.1893296846482002 2117 6 0.4118979021893949 2117 7 0.667784966143941 2117 8 0.22907452245448212 2117 9 -1.1774836675044185 2117 10-11 -0.27178091965804896 2117 12-13 -0.7135732545499067 2117 14-15 0.4484571109827655 2117 16-17 -0.5826319000388338 2117 18-19 -0.7429737971994044 2117 20-21 0.14002527999138437 2117 22-23 0.2787755147455684 2117 24-25 -1.3767083722517413 2117 26-27 -0.9748209450928869 2117 28-29 -0.7197500386349489 2117 30-31 -0.06237827540473262 2117 32-33 -1.0219649444628587 2117 34-35 0.7919628255262836 2117 36-37 -0.923136161941585 2117 38-39 0.4327162112597307 2117 40-41 0.49806580511309306 2117 42-43 0.4659740663572194 2117 44-45 -0.23726126841087591 2117 46-47 -0.46993534224040445 2117 48-49 -0.32263410760354105 2117 50-51 0.015982481867595766 2117 52-53 0.936053101200315 2117 54-55 -0.3599506934019274 2117 56-57 0.20768634449991197 2117 58-59 -0.6852049599563372 2117 60-61 0.08847895311839693 2117 62-63 -0.18399347311147807 2117 64-65 -0.48875041456715707 2117 66-67 -0.22871267802043604 2117 68-69 0.07723720075700413 2117 70-71 -0.2864158764287694 2117 72-73 1.0614068607662617 2117 74-75 0.8387429271657751 2117 76 0.7490212966036083 2118 1 0.36099662099363883 2118 2 0.30105538379071106 2118 3 -0.5859014344476705 2118 4 0.2799240339920388 2118 5 0.32905703804014763 2118 6 0.3729045973575751 2118 7 0.7966064552138761 2118 8 0.6413839521552163 2118 9 -1.509305882556447 2118 10-11 -0.4955769993547463 2118 12-13 -0.8321099973102548 2118 14-15 0.7089394857508537 2118 16-17 -2.5429368125176595 2118 18-19 -0.44536953810446533 2118 20-21 -0.3823591678589011 2118 22-23 0.5825102892877894 2118 24-25 0.7091107775425627 2118 26-27 -1.9711507022922063 2118 28-29 -0.09641374759680588 2118 30-31 -0.8369608357015821 2118 32-33 0.1553763477852712 2118 34-35 -0.591847773812276 2118 36-37 -2.469340888891658 2118 38-39 0.16879752125407776 2118 40-41 -0.5795900793750874 2118 42-43 0.686680688927197 2118 44-45 -2.299187540896156 2118 46-47 0.09562890717477757 2118 48-49 0.23796926417995934 2118 50-51 -0.9752492677492484 2118 52-53 -1.182596572107748 2118 54-55 -0.7858440546990195 2118 56-57 0.2624979924792612 2118 58-59 -0.6246419205113085 2118 60-61 -0.21472343455604204 2118 62-63 -0.6574789938019663 2118 64-65 0.042612011826328455 2118 66-67 -0.11661297647974322 2118 68-69 -1.0484948311862041 2118 70-71 -0.0875935807120598 2118 72-73 1.3072243972670279 2118 74-75 0.059110758092607796 2118 76 0.19871073759739488 2119 1 0.7021364235393008 2119 2 -0.9592918154570782 2119 3 0.11224068392045439 2119 4 -0.19488922017382748 2119 5 -0.049832411908482754 2119 6 -0.3240186670814893 2119 7 -0.1519579089796821 2119 8 0.36802984026441976 2119 9 0.09515860526371966 2119 10-11 0.12203369870029945 2119 12-13 0.17233881953082886 2119 14-15 0.16088181654781408 2119 16-17 0.027626123875577946 2119 18-19 0.36204283500413936 2119 20-21 -0.935448962569069 2119 22-23 0.09969612968757957 2119 24-25 1.0859455880107731 2119 26-27 0.7951601751085065 2119 28-29 0.731971619191178 2119 30-31 1.2561508983793175 2119 32-33 0.7100767709017077 2119 34-35 0.5732808152772435 2119 36-37 0.43502260817438554 2119 38-39 -0.8118401134728117 2119 40-41 -0.40135881054814604 2119 42-43 0.5474179214322845 2119 44-45 0.4945736682069395 2119 46-47 0.21807149044862228 2119 48-49 0.14928893474115768 2119 50-51 0.6696550522173226 2119 52-53 0.2748915440664419 2119 54-55 0.39198673355122793 2119 56-57 -0.6109327330870613 2119 58-59 0.33032142680983156 2119 60-61 0.6615977160420741 2119 62-63 -0.10125500327421122 2119 64-65 0.22335063730734106 2119 66-67 0.7175967708692497 2119 68-69 1.0098273293194424 2119 70-71 0.43966521680147963 2119 72-73 0.9725009900479336 2119 74-75 -0.020593582768597685 2119 76 0.24027365657843092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 3.0 21 4.0 22 8.0 23 17.0 24 34.0 25 73.0 26 128.0 27 237.0 28 447.0 29 698.0 30 1230.0 31 1960.0 32 3020.0 33 4891.0 34 7685.0 35 12217.0 36 21847.0 37 40487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.2321977512949 22.501789699751548 11.393018065439845 27.872994483513708 2 19.07188276413863 18.629721649050406 36.23089232324083 26.067503263570135 3 18.139133364214427 24.12409988630143 29.171053185665556 28.56571356381859 4 13.48275571651156 17.609592790668295 37.677390828315154 31.23026066450499 5 13.165873584031667 36.06034446456394 36.44565629342654 14.32812565797785 6 29.91956878763633 37.920579441613675 16.726323325051585 15.433528445698405 7 28.258306312376302 29.171053185665556 22.543900282140903 20.026740219817242 8 24.704173158714784 34.25590600918011 20.128858382111424 20.911062449993683 9 23.569292963321683 15.646186886764642 21.862761611993093 38.92175853792058 10-11 25.00684296963827 25.50216869499305 27.140270349938937 22.35071798542974 12-13 24.723649303069863 23.26925506379753 26.700214763970187 25.30688086916242 14-15 21.612203646776436 24.96631153408852 23.652987745820525 29.768497073314528 16-17 22.439150208447384 27.938792268497075 25.96591148355582 23.656146039499724 18-19 22.4101991830547 27.401355960752937 27.014464985050747 23.17397987114162 20-21 21.669052933002064 27.37661599359919 26.45439423927233 24.499936834126416 22-23 22.491788436434078 27.538215353518343 27.14184949677854 22.828146713269046 24-25 22.622857624120943 28.06828230934434 26.44386659367499 22.86499347285973 26-27 21.69326651787594 28.616772644965682 26.963932286183518 22.72602855097486 28-29 21.839600791678947 27.103949972628122 28.105129068935025 22.951320166757906 30-31 21.511138249041984 26.69442455889165 28.536762538425908 23.257674653640457 32-33 22.315450372678654 27.382406198677728 26.928138291152564 23.374005137491054 34-35 22.945003579399504 26.722322819724596 27.66875815892534 22.663915441950564 36-37 23.02343453909968 26.998673516654737 27.08868488651198 22.889207057733607 38-39 21.89434454878511 27.343453909967575 27.799827346612204 22.962374194635114 40-41 21.616414705015373 25.92116898976713 28.661515138754368 23.80090116646313 42-43 21.908556870341517 26.065924116730532 28.896281635575022 23.12923737735293 44-45 20.734198003958397 25.999073567187438 29.088411167726452 24.17831726112772 46-47 21.57535688718575 25.050006316587357 29.462668968711835 23.911967827515053 48-49 20.87053101444393 25.212132058786374 29.700593759211692 24.216743167558008 50-51 19.87408935865583 25.825893797111217 29.901671790120858 24.3983450541121 52-53 19.447719711963614 26.023287152061314 30.933907440939905 23.595085695035163 54-55 18.496546932244073 26.59125363203773 30.009580157493577 24.90261927822462 56-57 19.599317808565292 26.179096306901922 29.223165031372382 24.9984208531604 58-59 18.783951657051418 26.18804480565966 29.5905798627195 25.43742367456942 60-61 19.401924453615194 26.919189792394825 28.509390659872825 25.16949509411715 62-63 18.31020760517118 27.670337305764935 27.923000800101068 26.096454288962818 64-65 19.265591443129658 28.169874089358654 26.55388049016718 26.010653977344507 66-67 19.105044847770245 27.869309807554636 26.115930433317892 26.909714911357224 68-69 18.951867604328967 28.36779382658862 25.635343411799383 27.044995157283026 70-71 19.15715669347707 27.87246810123384 25.92064260748726 27.049732597801828 72-73 19.319808817955952 27.797721817492736 25.561649892618014 27.320819471933294 74-75 19.72670232029309 27.95142544321388 25.496378489914516 26.825493746578516 76 19.74354655324883 27.96774329388975 25.35793995030951 26.9307702025519 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.5 14 0.5 15 1.5 16 2.5 17 2.0 18 3.5 19 16.0 20 22.0 21 17.0 22 26.0 23 54.0 24 91.0 25 109.0 26 171.0 27 292.5 28 435.0 29 518.0 30 569.5 31 782.5 32 1105.5 33 1267.0 34 1538.0 35 2107.5 36 2591.0 37 2776.0 38 3204.0 39 4072.5 40 4994.5 41 5805.5 42 6135.0 43 6382.0 44 6952.5 45 7336.5 46 7397.0 47 7351.0 48 6976.0 49 6270.5 50 5894.0 51 5398.0 52 4451.0 53 3646.5 54 3293.0 55 3089.0 56 2494.5 57 1880.0 58 1656.0 59 1391.0 60 963.0 61 715.0 62 630.0 63 547.5 64 421.0 65 331.0 66 223.5 67 162.0 68 126.5 69 82.0 70 42.5 71 12.0 72 10.5 73 9.0 74 6.0 75 3.0 76 2.0 77 1.0 78 0.5 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 94988.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.579862719501406 #Duplication Level Percentage of deduplicated Percentage of total 1 58.72464068904686 23.83037857413568 2 16.37264567010844 13.287994272960796 3 8.628651481346962 10.504484777024466 4 4.846157837389094 7.866256790331411 5 2.9652882270533905 6.016549458879016 6 1.8678980957816635 4.54794289805028 7 1.2919628495823172 3.66993725523224 8 0.9962123177502206 3.234092727502421 9 0.7808851761531677 2.85193919231903 >10 3.491931717947388 22.21964879774287 >50 0.02594302910807866 0.6411336168779214 >100 0.005188605821615732 0.5232239861877289 >500 0.002594302910807866 0.8064176527561375 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 766 0.8064176527561375 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 389 0.409525413736472 RNA PCR Primer, Index 30 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 108 0.113698572451257 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 98 0.10317092685391839 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007369351918137028 0.0 2 0.0 0.0 0.0 0.012633174716806334 0.0 3 0.0 0.0 0.0 0.012633174716806334 0.0 4 0.0 0.0 0.0 0.02000252663494336 0.0 5 0.0 0.0 0.0 0.022108055754411084 0.0 6 0.0 0.0 0.0 0.022108055754411084 0.0 7 0.0 0.0 0.0 0.023160820314144945 0.0 8 0.0 0.0 0.0 0.025266349433612667 0.0 9 0.0 0.0 0.0 0.04000505326988672 0.0 10 0.0 0.0 0.0 0.050532698867225334 0.0 11 0.0 0.0 0.0 0.05895481534509622 0.0 12 0.0 0.0 0.0 0.062113109024297807 0.0 13 0.0 0.0 0.0 0.06527140270349939 0.0 14 0.0 0.0 0.0 0.07158799006190256 0.0 15 0.0 0.0 0.0 0.075799048300838 0.0 16 0.0 0.0 0.0 0.0810628710995073 0.0 17 0.0 0.0 0.0 0.08948498757737819 0.0 18 0.0 0.0 0.0 0.09264328125657978 0.0 19 0.0 0.0 0.0 0.09685433949551522 0.0 20 0.0 0.0 0.0 0.10317092685391839 0.0 21 0.0 0.0 0.0 0.10843474965258769 0.0 22 0.0 0.0 0.0 0.11475133701099086 0.0 23 0.0 0.0 0.0 0.11790963069019245 0.0 24 0.0 0.0 0.0 0.1189623952499263 0.0 25 0.0 0.0 0.0 0.12422621804859561 0.0 26 0.0 0.0 0.0 0.1273845117277972 0.0 27 0.0 0.0 0.0 0.1568619194003453 0.0 28 0.0 0.0 0.0 0.1863393270728934 0.0 29 0.0 0.0 0.0 0.21055291194677223 0.0 30 0.0 0.0 0.0 0.2305554385817156 0.0 31 0.0 0.0 0.0 0.27266602097107 0.0 32 0.0 0.0 0.0 0.32846254263696467 0.0 33 0.0 0.0 0.0 0.3600454794289805 0.0 34 0.0 0.0 0.0 0.42742241125194763 0.0 35 0.0 0.0 0.0 0.5200656925085274 0.0 36 0.0 0.0 0.0 0.660083378953131 0.0 37 0.0 0.0 0.0 1.0443424432559902 0.0 38 0.0 0.0 0.0 1.5507221964879774 0.0 39 0.0 0.0 0.0 2.098159767549585 0.0 40 0.0 0.0 0.0 2.7624542047416516 0.0 41 0.0 0.0 0.0 3.7415252452941425 0.0 42 0.0 0.0 0.0 4.746915399839979 0.0 43 0.0 0.0 0.0 5.783888491177833 0.0 44 0.0 0.0 0.0 6.8398113445908955 0.0 45 0.0 0.0 0.0 7.776771802754032 0.0 46 0.0 0.0 0.0 8.837958478965763 0.0 47 0.0 0.0 0.0 9.76649682065103 0.0 48 0.0 0.0 0.0 10.595022529161579 0.0 49 0.0 0.0 0.0 11.316166252579274 0.0 50 0.0 0.0 0.0 12.068892912788984 0.0 51 0.0 0.0 0.0 12.883732682022993 0.0 52 0.0 0.0 0.0 13.843853960500274 0.0 53 0.0 0.0 0.0 14.88714363919653 0.0 54 0.0 0.0 0.0 15.929380553333052 0.0 55 0.0 0.0 0.0 16.863182717816986 0.0 56 0.0 0.0 0.0 17.66012548953552 0.0 57 0.0 0.0 0.0 18.397060681349224 0.0 58 0.0 0.0 0.0 19.10241293637091 0.0 59 0.0 0.0 0.0 19.785657135638186 0.0 60 0.0 0.0 0.0 20.440476691792647 0.0 61 0.0 0.0 0.0 21.0763464858719 0.0 62 0.0 0.0 0.0 21.830125910641343 0.0 63 0.0 0.0 0.0 22.6091716848444 0.0 64 0.0 0.0 0.0 23.400850633764264 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGAG 15 0.0022186064 70.0 5 CGAGGCC 15 0.0022186064 70.0 8 CGGGCGA 15 0.0022186064 70.0 4 AGACCGG 15 0.0022186064 70.0 10 TGGGCCG 15 0.0022186064 70.0 10 GCCCCCC 20 0.0069325096 52.5 11 CGGAATC 20 0.0069325096 52.5 20 CTGTAGC 20 0.0069325096 52.5 1 CGTGCTT 20 0.0069325096 52.5 65 AACCCGT 20 0.0069325096 52.5 38 TCGGGCG 20 0.0069325096 52.5 3 ATTGTTC 20 0.0069325096 52.5 8 CTGCGTG 20 0.0069325096 52.5 62 CGTTACA 20 0.0069325096 52.5 14 AGCGGAA 20 0.0069325096 52.5 18 CGTAGCG 20 0.0069325096 52.5 15 GAGCGGT 20 0.0069325096 52.5 6 TGTTCGT 20 0.0069325096 52.5 10 GCGTGCT 20 0.0069325096 52.5 64 TGGTCCA 20 0.0069325096 52.5 21 >>END_MODULE