FastQCFastQC Report
Wed 25 May 2016
SRR1780674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52381
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTC150.002213747569.9942712
TGAAGGT150.002213747569.9942728
GTCTCGA150.002213747569.9942760
TTGAGGC150.002213747569.9942711
TATTGAG150.002213747569.994279
ACGTCTC150.002213747569.9942758
GCGGATG150.002213747569.9942736
GGCGCCA150.002213747569.9942715
CGCCATT252.3709038E-455.9954217
TGTTTTG200.006917455252.495727
CTTCGGC200.006917455252.495724
ATGATTC200.006917455252.495740
AATTTAC200.006917455252.495753
CAAGTGA200.006917455252.495728
TTCAGCC200.006917455252.495744
CGTCTCG200.006917455252.495759
GCCATTG200.006917455252.495718
AATATTG200.006917455252.49577
AGCGGAT200.006917455252.495735
CGGATGA200.006917455252.495737
Produced by FastQC (version 0.11.5)