##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780674_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52381 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.573490387736012 34.0 31.0 34.0 12.0 34.0 2 30.723525705885724 34.0 32.0 34.0 21.0 34.0 3 30.84446650503045 34.0 32.0 34.0 21.0 34.0 4 31.215478895019185 34.0 33.0 34.0 23.0 34.0 5 31.13926805521086 34.0 34.0 34.0 21.0 34.0 6 34.101506271357934 38.0 34.0 38.0 23.0 38.0 7 32.87464920486436 37.0 32.0 38.0 11.0 38.0 8 31.9959718218438 37.0 31.0 38.0 11.0 38.0 9 32.91621007617266 37.0 33.0 38.0 12.0 38.0 10-11 33.23809205627995 37.0 34.0 38.0 21.0 38.0 12-13 33.59422309616082 37.0 34.0 38.0 21.0 38.0 14-15 33.25448158683493 37.0 34.0 38.0 21.0 38.0 16-17 33.141854871041026 37.0 34.0 38.0 21.0 38.0 18-19 33.106555812221984 37.0 33.5 38.0 16.0 38.0 20-21 33.16870621026708 37.0 34.0 38.0 16.0 38.0 22-23 33.13659533036788 37.0 34.0 38.0 16.0 38.0 24-25 33.538716328439705 37.5 34.0 38.0 21.0 38.0 26-27 33.58070674481205 38.0 34.0 38.0 21.0 38.0 28-29 33.66817166529849 38.0 34.0 38.0 21.0 38.0 30-31 33.53541360416945 38.0 34.0 38.0 21.0 38.0 32-33 33.317080620835796 37.0 34.0 38.0 21.0 38.0 34-35 33.20695481185926 37.0 34.0 38.0 21.0 38.0 36-37 33.16272121570799 37.0 34.0 38.0 16.0 38.0 38-39 33.64316259712491 37.5 34.0 38.0 21.0 38.0 40-41 33.151562589488556 37.0 34.0 38.0 16.0 38.0 42-43 33.48805864721941 37.0 34.0 38.0 21.0 38.0 44-45 32.62029170882572 37.0 31.5 38.0 11.0 38.0 46-47 33.27602565815849 37.0 34.0 38.0 21.0 38.0 48-49 33.04919722800252 37.0 33.5 38.0 16.0 38.0 50-51 33.06635993967278 37.0 33.5 38.0 16.0 38.0 52-53 33.010900899181 37.0 33.5 38.0 18.5 38.0 54-55 32.710887535556786 37.0 32.0 38.0 11.0 38.0 56-57 32.78143792596552 37.0 32.0 38.0 15.0 38.0 58-59 32.715793894732826 37.0 32.0 38.0 15.0 38.0 60-61 32.68651801225635 37.0 31.5 38.0 11.0 38.0 62-63 32.79233882514652 37.0 32.0 38.0 15.0 38.0 64-65 32.77903247360684 37.0 32.0 38.0 14.5 38.0 66-67 32.74233977969111 37.0 31.5 38.0 14.0 38.0 68-69 32.323619251255224 37.0 31.0 38.0 14.5 38.0 70-71 32.25184704377541 37.0 31.0 38.0 11.0 38.0 72-73 32.302962906397354 37.0 31.0 38.0 11.0 38.0 74-75 31.983123651705768 37.0 30.5 38.0 11.0 38.0 76 31.188121647162138 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.7577176951014977 1101 2 -1.4909238864535013 1101 3 -0.7281739557029567 1101 4 -3.086277997621629 1101 5 -2.360956756701224 1101 6 -2.553358703252222 1101 7 -2.349678995646027 1101 8 -0.9531173617581743 1101 9 -1.9144860580139174 1101 10-11 -2.0479757745076377 1101 12-13 -2.730441502751436 1101 14-15 -1.7851881479976726 1101 16-17 -2.95813066820633 1101 18-19 -0.6252820864742148 1101 20-21 -1.5430612146326368 1101 22-23 -1.389273971046869 1101 24-25 -2.7642650532319877 1101 26-27 -1.4141558259531024 1101 28-29 -1.2435194416303617 1101 30-31 -1.6543921276676876 1101 32-33 -0.6412785549755498 1101 34-35 -1.5254406772296427 1101 36-37 -0.8008097355421313 1101 38-39 -2.3490569574847058 1101 40-41 -0.860128256183593 1101 42-43 -0.946814472009379 1101 44-45 -1.2173415862960049 1101 46-47 -0.44913346399417264 1101 48-49 -2.0851107989621056 1101 50-51 -1.8027430607855806 1101 52-53 -1.5427660476182865 1101 54-55 -2.03881990076583 1101 56-57 -1.5299838963303785 1101 58-59 -2.103176453442174 1101 60-61 -1.3083022779084388 1101 62-63 -1.9457610745674216 1101 64-65 -1.119265821090373 1101 66-67 -1.8947572700342619 1101 68-69 0.5923968882312991 1101 70-71 -0.4811990080612958 1101 72-73 -1.5563590278568 1101 74-75 -2.0003341292725487 1101 76 -2.639276640419773 1102 1 -0.0440080176821418 1102 2 -0.7880509025825333 1102 3 -0.5767122621545688 1102 4 -0.36626791697646865 1102 5 -0.04064425670122418 1102 6 0.50158081287681 1102 7 1.778345197902361 1102 8 1.6330721543708577 1102 9 1.6825401516635026 1102 10-11 -0.14991629870118572 1102 12-13 -2.645134043074016 1102 14-15 -1.1155812931589608 1102 16-17 -2.317757684335362 1102 18-19 -0.7156298687322789 1102 20-21 -0.4266297630197329 1102 22-23 -1.202025987175901 1102 24-25 -0.044632996780372025 1102 26-27 -5.559569132404718 1102 28-29 -0.5808430303400414 1102 30-31 0.3457338803968284 1102 32-33 -6.150351135620717 1102 34-35 -1.1466604352941587 1102 36-37 -0.5641665903808359 1102 38-39 0.1350660263862622 1102 40-41 -0.2770889416674649 1102 42-43 -0.037918302654539104 1102 44-45 -6.889342594360521 1102 46-47 -0.05876048012320467 1102 48-49 0.15115432200563816 1102 50-51 -6.276407375301709 1102 52-53 -1.3411531443924787 1102 54-55 0.04195126858900977 1102 56-57 -1.6481794608465066 1102 58-59 0.7496461272029862 1102 60-61 -0.24920449565037472 1102 62-63 -2.8017338568254857 1102 64-65 -0.31294021625166124 1102 66-67 0.03984454448186625 1102 68-69 -8.301252305317085 1102 70-71 -0.2283008225774239 1102 72-73 0.1499161737561039 1102 74-75 -6.347612355079001 1102 76 -2.055859301710097 1103 1 -0.10872432973888024 1103 2 -0.19579691322083193 1103 3 -0.7253713933602413 1103 4 0.22886727805899199 1103 5 0.24365317237678852 1103 6 -2.165861563009713 1103 7 0.43858455960448595 1103 8 1.2677131118176632 1103 9 -1.1500728802513898 1103 10-11 0.06670050094420787 1103 12-13 -0.7426240696697661 1103 14-15 -1.314329076846903 1103 16-17 -0.41034412050557734 1103 18-19 -0.5917657286613576 1103 20-21 -1.1417228481261148 1103 22-23 -1.0480477070340584 1103 24-25 -0.6713229347236407 1103 26-27 -0.6985873061635814 1103 28-29 0.6066292657592456 1103 30-31 -0.7416719263407643 1103 32-33 -4.473267802287385 1103 34-35 -0.70411832536508 1103 36-37 -0.35145604073544234 1103 38-39 -0.1133271473726083 1103 40-41 0.19517945372260925 1103 42-43 -0.05096683988858075 1103 44-45 -4.872083064218678 1103 46-47 -0.30648876026504723 1103 48-49 0.3796283911545686 1103 50-51 0.5009917824997103 1103 52-53 -1.2392027897825493 1103 54-55 -0.03392971545354584 1103 56-57 0.7418360533378916 1103 58-59 -0.06728649691049071 1103 60-61 0.22289767633544244 1103 62-63 -0.09841492952052278 1103 64-65 0.005848569209334187 1103 66-67 -0.35806658140465686 1103 68-69 -2.481327659927011 1103 70-71 0.38547909763533994 1103 72-73 0.5098729556709927 1103 74-75 -0.9882373550790007 1103 76 -1.3263047804334995 1104 1 -1.002341351015474 1104 2 0.19545604186190957 1104 3 0.597766904512099 1104 4 0.06515569413464206 1104 5 -0.2915123122567813 1104 6 0.17692803509903143 1104 7 0.809595197902361 1104 8 2.173002709926415 1104 9 1.1278526516635026 1104 10-11 -0.4871558820345143 1104 12-13 -0.6290750152962374 1104 14-15 -0.014018793158960818 1104 16-17 -0.26133407322424773 1104 18-19 0.990533325712164 1104 20-21 1.65843968142471 1104 22-23 0.25543929060187054 1104 24-25 0.7917385309973994 1104 26-27 0.7937294787063962 1104 28-29 0.04893127521551577 1104 30-31 -0.5878598696031716 1104 32-33 -0.9975733578429384 1104 34-35 0.18580484248361984 1104 36-37 0.3794097985080498 1104 38-39 1.0977396374973765 1104 40-41 1.4429631416658637 1104 42-43 1.575362947345461 1104 44-45 -2.3116516221382994 1104 46-47 0.6191909087656811 1104 48-49 1.138567516450081 1104 50-51 1.4879155413649627 1104 52-53 0.617180188940857 1104 54-55 -0.1277535925220974 1104 56-57 0.708591372486822 1104 58-59 0.9718683494252076 1104 60-61 0.6244934210162896 1104 62-63 0.6665821153967428 1104 64-65 0.3775979781927816 1104 66-67 0.5611119055929805 1104 68-69 -3.0703495275393067 1104 70-71 -0.23915151702186677 1104 72-73 0.7979196459783324 1104 74-75 -0.998654021745665 1104 76 0.4189670871787925 1105 1 -0.6232563836952139 1105 2 -0.20650474245181272 1105 3 -0.4500272131349625 1105 4 0.4622145176640551 1105 5 0.31387984460596385 1105 6 0.0984966625500121 1105 7 1.36474225672589 1105 8 0.3294569582924254 1105 9 0.4583265078726555 1105 10-11 0.6515287584883538 1105 12-13 -1.393576649283169 1105 14-15 0.048276958475028664 1105 16-17 1.3630531816777136 1105 18-19 0.8749287505487615 1105 20-21 0.6353596160652302 1105 22-23 0.2578902709940323 1105 24-25 0.02294768132420444 1105 26-27 -0.07391758011713989 1105 28-29 -0.7216978097518094 1105 30-31 0.10535908464519395 1105 32-33 0.8208498447714376 1105 34-35 1.1592525549019186 1105 36-37 1.279123850795635 1105 38-39 0.24990467017710927 1105 40-41 0.16722784754822584 1105 42-43 0.83312438525396 1105 44-45 1.6626130837440556 1105 46-47 0.916985026412739 1105 48-49 0.5341966014174062 1105 50-51 0.23280933221463584 1105 52-53 0.24953313011732803 1105 54-55 -0.2858418278162205 1105 56-57 0.20491490189859007 1105 58-59 0.2435186762225925 1105 60-61 1.074656819709105 1105 62-63 0.8240576055928202 1105 64-65 0.2387090893038959 1105 66-67 1.0762262846779436 1105 68-69 1.3077141979508866 1105 70-71 0.9914448882068854 1105 72-73 1.0093167048018543 1105 74-75 -0.5699367014842309 1105 76 -0.4486799716447365 1106 1 -0.35965549969652955 1106 2 0.1790691513742999 1106 3 -0.550464510356008 1106 4 0.06375681323935822 1106 5 0.08328784761532404 1106 6 0.026648258919976797 1106 7 -0.20614221216958128 1106 8 0.5153788090471139 1106 9 1.6795612847570283 1106 10-11 0.18902817072327593 1106 12-13 0.4715195090842528 1106 14-15 0.5729866025244874 1106 16-17 1.0023299955207534 1106 18-19 0.6679413992533298 1106 20-21 0.14137833050544657 1106 22-23 0.14043129699676626 1106 24-25 0.6011909690469608 1106 26-27 0.5944888711924037 1106 28-29 -0.562154855879605 1106 30-31 -0.4570145458621653 1106 32-33 0.815701022652668 1106 34-35 0.5865342589504436 1106 36-37 0.9399363772450613 1106 38-39 0.6977346414941721 1106 40-41 0.961698153656279 1106 42-43 0.8509924437483392 1106 44-45 1.5825829901718507 1106 46-47 1.1021545378624111 1106 48-49 0.7271716331567148 1106 50-51 0.766617579734266 1106 52-53 0.3935590858233553 1106 54-55 0.9469366552796572 1106 56-57 1.2947724276426982 1106 58-59 0.26538353727492847 1106 60-61 1.2932584090258814 1106 62-63 1.2413473032464566 1106 64-65 0.8845923826691973 1106 66-67 1.7732921613883406 1106 68-69 1.8427449968411835 1106 70-71 1.1717947259837302 1106 72-73 1.1658503014539505 1106 74-75 0.04773386794258272 1106 76 0.6289990615992558 1107 1 0.14731851293010223 1107 2 0.8156374477576023 1107 3 -0.22153581657633836 1107 4 0.49996295037047034 1107 5 0.39244460384299273 1107 6 0.5805561530128642 1107 7 0.21308159246018477 1107 8 -0.13552903610533207 1107 9 -0.7484228585405788 1107 10-11 -0.2735079228508397 1107 12-13 0.2519482273341467 1107 14-15 0.23321760139886294 1107 16-17 0.1741109381136141 1107 18-19 0.7055276567779245 1107 20-21 0.8136976179326467 1107 22-23 -0.46366501778814495 1107 24-25 0.4876002090019398 1107 26-27 0.7557476192959598 1107 28-29 -0.2955273415645294 1107 30-31 0.13110271543084195 1107 32-33 1.1355757351275813 1107 34-35 0.675459037494953 1107 36-37 0.043029413020526874 1107 38-39 0.7525724039372861 1107 40-41 1.2554631416658637 1107 42-43 1.009461586801244 1107 44-45 0.946993502578259 1107 46-47 1.214570727359792 1107 48-49 0.9345567454750281 1107 50-51 1.145441051569044 1107 52-53 1.1494931141109248 1107 54-55 0.9489187430788064 1107 56-57 0.9862274269085987 1107 58-59 1.2917379639376847 1107 60-61 -0.19098276945990023 1107 62-63 0.1418513897731586 1107 64-65 0.7987997922517422 1107 66-67 0.7114803863185983 1107 68-69 1.4088880008053692 1107 70-71 1.3011687777627117 1107 72-73 1.1116242945044021 1107 74-75 0.8655041415196365 1107 76 0.6372210554327609 1108 1 -0.4775186559800133 1108 2 -0.07522953733430526 1108 3 -0.32820827279286746 1108 4 -0.6860263389622858 1108 5 -0.4230134942898758 1108 6 -0.10912397435723165 1108 7 2.1832654106683194 1108 8 1.6577840338034804 1108 9 0.40311860911030806 1108 10-11 0.48514021725625867 1108 12-13 0.530425575720308 1108 14-15 0.8558836891105415 1108 16-17 -0.48835830490273224 1108 18-19 0.6210002287854479 1108 20-21 0.37246154903700557 1108 22-23 0.026420378072323558 1108 24-25 0.06271961846785956 1108 26-27 -0.24823269623450983 1108 28-29 0.1420902586670465 1108 30-31 0.6838599885528609 1108 32-33 0.5515548927480793 1108 34-35 0.6327611072590358 1108 36-37 0.6769127535907984 1108 38-39 0.7909281717763363 1108 40-41 0.28383193599211154 1108 42-43 0.16179911755822474 1108 44-45 0.34068289322812717 1108 46-47 0.7183339347704134 1108 48-49 0.7413305188141521 1108 50-51 0.6960272434926154 1108 52-53 0.2146979194373131 1108 54-55 0.4093326958939727 1108 56-57 1.603538180997468 1108 58-59 0.035550382522139046 1108 60-61 0.8257345557680651 1108 62-63 0.17108861657877128 1108 64-65 0.34272800183345 1108 66-67 0.11001852497831521 1108 68-69 1.6924312053212134 1108 70-71 1.9847698777771825 1108 72-73 0.9247665726922705 1108 74-75 1.384103070452916 1108 76 1.6453264252402597 1109 1 -1.385320074419731 1109 2 -0.8270025869796953 1109 3 -0.519697634494996 1109 4 -0.5158135730048379 1109 5 0.1727311865611867 1109 6 0.12491857883425439 1109 7 -0.887656575147286 1109 8 0.7428904167822026 1109 9 0.708083148117403 1109 10-11 0.421310430022217 1109 12-13 -0.2568084668328865 1109 14-15 -0.7363148924497409 1109 16-17 -0.48481220561195926 1109 18-19 -0.0385742392996562 1109 20-21 0.02139771924976941 1109 22-23 0.3952147043134602 1109 24-25 0.7896699730777854 1109 26-27 -0.1383036182203199 1109 28-29 -0.38273243636840704 1109 30-31 -0.24521802563153727 1109 32-33 0.12956907714524135 1109 34-35 -0.3317778998331633 1109 36-37 -0.10677518967161603 1109 38-39 0.1384459022727853 1109 40-41 -0.21262196471710837 1109 42-43 0.31782748635254876 1109 44-45 -0.10257951811939137 1109 46-47 -1.7887582638111468 1109 48-49 -0.9111517506893989 1109 50-51 0.29177192434368493 1109 52-53 -0.168280803966951 1109 54-55 0.34550290865993105 1109 56-57 -0.5276674927614025 1109 58-59 -0.9786340146409884 1109 60-61 -0.33029381302625893 1109 62-63 -1.7757198940595273 1109 64-65 -1.1750024946204576 1109 66-67 -1.287144595589055 1109 68-69 0.3910127656049056 1109 70-71 -0.5045918243504737 1109 72-73 -1.128424916669431 1109 74-75 0.6890676094600039 1109 76 -0.8085742839795991 1110 1 1.0140905738671542 1110 2 0.09608201995267862 1110 3 0.08338897023979897 1110 4 0.2110868365446592 1110 5 0.5932685954114518 1110 6 0.3490720100599063 1110 7 0.7611797049446167 1110 8 1.7943837740891695 1110 9 -0.8730276300266375 1110 10-11 -1.8083119853209055 1110 12-13 -0.5939126522289442 1110 14-15 -0.6200927368209292 1110 16-17 0.36655129453787794 1110 18-19 -0.0780308370421352 1110 20-21 -0.5038744813295892 1110 22-23 0.0991403860635387 1110 24-25 -0.03709558480854014 1110 26-27 -1.3138605056441577 1110 28-29 -0.4621700549879293 1110 30-31 -0.12043029213838707 1110 32-33 1.3412861883229468 1110 34-35 0.9053862196354174 1110 36-37 -0.4166093720709796 1110 38-39 0.29252641371020616 1110 40-41 0.5911438928395754 1110 42-43 0.39666576424686895 1110 44-45 1.1342599584563757 1110 46-47 -0.7881838956161644 1110 48-49 1.0774857480619744 1110 50-51 0.3639425366701161 1110 52-53 -0.32530807396994277 1110 54-55 -0.7111847349321181 1110 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2116 48-49 0.15958359832143287 2116 50-51 -0.674286839587424 2116 52-53 0.7105385849308306 2116 54-55 -0.6439921054711419 2116 56-57 -1.284986321748761 2116 58-59 -1.5074027449774619 2116 60-61 -2.294992794522557 2116 62-63 0.19412493076850268 2116 64-65 0.6643565412671393 2116 66-67 -0.9217097318339214 2116 68-69 -0.41603144065543063 2116 70-71 -3.932630004908251 2116 72-73 0.04538727338016457 2116 74-75 -2.8265832197406553 2116 76 -3.0620270648679906 2117 1 0.4198808712067468 2117 2 0.9250856714915408 2117 3 0.8519335711787654 2117 4 0.2299705089494566 2117 5 -0.259104904849373 2117 6 -0.8971460389750447 2117 7 -2.0714233206161587 2117 8 -1.3302380308143285 2117 9 1.8792415405523926 2117 10-11 -0.3739614375900757 2117 12-13 0.9336564661852478 2117 14-15 -0.017953978344145582 2117 16-17 0.4606566675164885 2117 18-19 0.5659962886751302 2117 20-21 -1.141328837093809 2117 22-23 0.22673558689817241 2117 24-25 0.6778496421085194 2117 26-27 0.90298873796565 2117 28-29 0.37045905299329007 2117 30-31 0.07093642669312317 2117 32-33 0.693398864379283 2117 34-35 0.5492307684095437 2117 36-37 -0.9476735348252845 2117 38-39 0.6215822300899632 2117 40-41 -0.7334257472230163 2117 42-43 -0.3945444600619439 2117 44-45 1.01010763712096 2117 46-47 -0.19238316530839228 2117 48-49 0.38278047941304294 2117 50-51 0.9807396154390275 2117 52-53 1.3523653741260375 2117 54-55 -0.08192025918877022 2117 56-57 -1.0427975164020609 2117 58-59 -0.8878538727970131 2117 60-61 1.3513452728681443 2117 62-63 -0.21559381052919235 2117 64-65 0.3387090893038902 2117 66-67 -1.815276983295913 2117 68-69 -0.72845137939116 2117 70-71 0.39209848297812755 2117 72-73 1.206484460793142 2117 74-75 0.2969478301061841 2117 76 -1.108810690598986 2118 1 -0.6097974913663506 2118 2 -0.545041034161482 2118 3 -0.56561028847036 2118 4 -0.20385015381857485 2118 5 -0.0977988619643817 2118 6 0.2836531812978649 2118 7 -0.14106269683448147 2118 8 -4.135842319313351 2118 9 -1.3885947167575488 2118 10-11 -1.3988883381748707 2118 12-13 -0.8920501614950709 2118 14-15 -0.7623740563168511 2118 16-17 -0.9102741317037797 2118 18-19 -1.236805855574385 2118 20-21 -2.2218600261776267 2118 22-23 -0.532206908228531 2118 24-25 -0.5255129309908995 2118 26-27 0.09709205180580938 2118 28-29 -0.48195322770846616 2118 30-31 -0.7720703959189592 2118 32-33 -0.6125221882522922 2118 34-35 -0.07223609318889856 2118 36-37 -0.7545448798545209 2118 38-39 -0.6860688420347927 2118 40-41 -0.9232649285095746 2118 42-43 -0.9049002105492789 2118 44-45 -0.07334752857104831 2118 46-47 -1.2302242959126772 2118 48-49 -1.846995349046992 2118 50-51 -1.1573695463543388 2118 52-53 -0.9595741970240539 2118 54-55 -1.0396687972004628 2118 56-57 0.08578435494296599 2118 58-59 0.7858303377293012 2118 60-61 -0.834936403545111 2118 62-63 -0.12636379103601314 2118 64-65 -0.8332938346727126 2118 66-67 -2.0697360476233975 2118 68-69 0.4594384841565997 2118 70-71 -1.9279307567879513 2118 72-73 -2.5871315236123174 2118 74-75 -0.830342618236898 2118 76 -0.21334285434167555 2119 1 1.1754364267623032 2119 2 1.0287893751952453 2119 3 0.5186002378454297 2119 4 0.8670075459864961 2119 5 1.1483025025580353 2119 6 1.8435947017656957 2119 7 -0.7603122095050452 2119 8 1.8030953025190044 2119 9 0.5903526516635011 2119 10-11 1.118631155002518 2119 12-13 1.1892120217407935 2119 14-15 0.6005645401743678 2119 16-17 0.26065666751648564 2119 18-19 0.05488517756401734 2119 20-21 0.8883007925358228 2119 22-23 1.3896985498611372 2119 24-25 0.6408126050714813 2119 26-27 0.8622479972249124 2119 28-29 1.314903497437733 2119 30-31 0.459825315582016 2119 32-33 1.060065531045943 2119 34-35 1.7010826202613956 2119 36-37 0.8745486873969384 2119 38-39 0.4882488967566374 2119 40-41 0.007314993517717028 2119 42-43 0.22767776216027613 2119 44-45 -0.73433680732348 2119 46-47 0.7594686865434639 2119 48-49 -0.45055285392028566 2119 50-51 1.806665541364957 2119 52-53 0.0782913000519656 2119 54-55 0.3810427037741917 2119 56-57 1.6720172984127544 2119 58-59 1.73807205312891 2119 60-61 0.8587526802755505 2119 62-63 0.7325543376189572 2119 64-65 1.8646350152298226 2119 66-67 0.9995378315189072 2119 68-69 -0.44326619420597524 2119 70-71 -0.06716077628112771 2119 72-73 -1.7083303540216725 2119 74-75 0.345095978254335 2119 76 0.3134115316232382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 10.0 13 73.0 14 232.0 15 448.0 16 602.0 17 818.0 18 841.0 19 785.0 20 756.0 21 625.0 22 542.0 23 563.0 24 519.0 25 581.0 26 726.0 27 874.0 28 942.0 29 1175.0 30 1492.0 31 1801.0 32 2332.0 33 2919.0 34 4103.0 35 6101.0 36 10275.0 37 12245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.98088130637995 27.071993229597428 10.549902867804043 28.397222596218576 2 16.58616673984842 23.36916057349039 36.23451251407953 23.81016017258166 3 15.696531184971649 27.00215727076612 30.948244592504913 26.353066951757313 4 11.824898341001507 19.316164258032494 38.23714705713904 30.62179034382696 5 11.078444474141387 37.58423855978313 36.62205761631126 14.715259349764228 6 24.694068459937764 41.94268914300987 17.7144384414196 15.648803955632767 7 23.951432771424752 30.239972509115905 22.931979152746223 22.87661556671312 8 21.39898054638132 35.72287661556671 20.01107271720662 22.867070120845344 9 22.481434107787173 16.10316717893893 22.452797770183846 38.96260094309005 10-11 23.055115404440542 26.170748935682788 27.558656764857485 23.21547889501919 12-13 22.55779767472939 23.747160229854337 26.63083942650961 27.064202668906667 14-15 20.11989080009927 25.426204157996217 23.829251064317212 30.62465397758729 16-17 21.73688933010061 27.523338615146713 25.95502185907104 24.78475019568164 18-19 21.235753422042343 26.528703155724404 26.427521429525974 25.808021992707282 20-21 20.566617666711213 27.43552051316317 26.19938527328611 25.798476546839506 22-23 21.83425287795193 26.917202802542906 26.647066684484834 24.601477635020334 24-25 21.921116435348694 27.68752028407247 25.765067486302286 24.62629579427655 26-27 20.249708863901034 28.224928886428284 26.911475535022237 24.61388671464844 28-29 21.381798743819324 27.068020847253777 28.015883621923983 23.53429678700292 30-31 21.408525992249096 27.12433897787366 27.505202267997937 23.961932761879307 32-33 20.167618029438156 28.497928638246695 26.878066474485024 24.45638685783013 34-35 21.39229873427388 27.57011129989882 26.747293866096484 24.290296099730817 36-37 22.137798056547222 26.758748401137815 26.841793780187473 24.26165976212749 38-39 21.01048089956282 26.92770279299746 28.128519883163744 23.933296424275976 40-41 21.05438995055459 26.346385139649875 28.244019778163835 24.3552051316317 42-43 20.34039059964491 26.326339703327545 28.25451976861839 25.078749928409156 44-45 19.970027299975182 26.35974876386476 28.294610641263052 25.375613294897004 46-47 20.739390236917966 26.548748592046735 28.233519787709284 24.478341383326015 48-49 19.708482083198106 26.880930108245355 29.049655409404174 24.360932399152365 50-51 19.989118191710734 26.953475496840458 28.066474485023196 24.990931826425612 52-53 19.198755273858843 27.153929860063762 28.25165613485806 25.395658731219335 54-55 18.76157385311468 27.597793092915367 28.216337985147288 25.424295068822666 56-57 19.265573394933277 26.827475611385807 27.948110956262767 25.958840037418145 58-59 19.57675493022279 26.606975811840172 27.78488383192379 26.031385426013248 60-61 19.631163971669118 26.251885225558887 27.881292835188333 26.235657967583663 62-63 19.516618621255798 26.131612607624906 27.739065691758462 26.612703079360838 64-65 20.25066340848781 26.756839311964264 26.712930260972488 26.279567018575438 66-67 20.28216337985147 26.825566522212252 25.757431129608065 27.13483896832821 68-69 19.664573032206334 27.3744296596094 26.097249002500906 26.86374830568336 70-71 20.56852675588477 27.619747618411257 24.83247742502052 26.979248200683454 72-73 20.55993585460377 27.328611519444074 24.84584104923541 27.265611576716747 74-75 20.500754090223555 27.733338424237797 25.138886237376145 26.627021248162503 76 21.06298085183559 27.10333899696455 25.060613581260384 26.77306656993948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.5 15 3.5 16 3.0 17 2.0 18 4.0 19 5.5 20 6.0 21 7.0 22 12.0 23 31.5 24 51.0 25 56.0 26 77.5 27 134.5 28 199.5 29 229.0 30 286.0 31 403.0 32 590.5 33 718.0 34 868.0 35 1155.0 36 1373.5 37 1455.0 38 1698.5 39 2222.5 40 2799.0 41 3239.0 42 3383.0 43 3458.5 44 3786.0 45 4089.0 46 4140.0 47 4058.5 48 3871.0 49 3502.0 50 3239.0 51 3066.5 52 2581.0 53 2081.0 54 1894.0 55 1761.0 56 1398.0 57 1039.0 58 910.0 59 710.0 60 470.5 61 360.0 62 289.0 63 251.5 64 178.5 65 143.0 66 122.0 67 101.0 68 82.5 69 55.5 70 38.0 71 29.0 72 24.0 73 18.5 74 14.5 75 11.0 76 8.5 77 5.5 78 9.0 79 13.0 80 8.0 81 2.0 82 1.5 83 2.0 84 2.0 85 1.5 86 0.5 87 0.0 88 0.5 89 1.0 90 1.5 91 3.0 92 4.0 93 2.5 94 1.0 95 0.5 96 0.0 97 0.0 98 1.0 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7445447776865658 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.23067524484069 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63880178797785 43.28859701036635 2 9.316832343718726 10.664172123479888 3 5.177129895256521 8.888719192073461 4 3.3224364533991593 7.605811267444302 5 1.9514310494362535 5.584085832649243 6 1.3009540329575022 4.467268666119394 7 0.857295349923277 3.434451423225979 8 0.6104476616185203 2.7949065500849546 9 0.4503302421775969 2.3195433458696857 >10 1.374341183534592 10.952444588686737 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.015272713388442374 0.0 2 0.0 0.0 0.0 0.02290907008266356 0.0 3 0.0 0.0 0.0 0.02290907008266356 0.0 4 0.0 0.0 0.0 0.040090872644661234 0.0 5 0.0 0.0 0.0 0.04199996181821653 0.0 6 0.0 0.0 0.0 0.04199996181821653 0.0 7 0.0 0.0 0.0 0.04772722933888242 0.0 8 0.0 0.0 0.0 0.04772722933888242 0.0 9 0.0 0.0 0.0 0.08399992363643306 0.0 10 0.0 0.0 0.0 0.1126362612397625 0.0 11 0.0 0.0 0.0 0.1164544395868731 0.0 12 0.0 0.0 0.0 0.13172715297531548 0.0 13 0.0 0.0 0.0 0.13936350966953667 0.0 14 0.0 0.0 0.0 0.14890895553731315 0.0 15 0.0 0.0 0.0 0.16227257975220022 0.0 16 0.0 0.0 0.0 0.16609075809931081 0.0 17 0.0 0.0 0.0 0.1756362039670873 0.0 18 0.0 0.0 0.0 0.20045436322330615 0.0 19 0.0 0.0 0.0 0.21190889826463793 0.0 20 0.0 0.0 0.0 0.21572707661174853 0.0 21 0.0 0.0 0.0 0.2348179683473015 0.0 22 0.0 0.0 0.0 0.25390886008285446 0.0 23 0.0 0.0 0.0 0.26918157347129684 0.0 24 0.0 0.0 0.0 0.27109066264485215 0.0 25 0.0 0.0 0.0 0.28636337603329454 0.0 26 0.0 0.0 0.0 0.2997270002481816 0.0 27 0.0 0.0 0.0 0.31690880281017925 0.0 28 0.0 0.0 0.0 0.35890876462839577 0.0 29 0.0 0.0 0.0 0.37609056719039347 0.0 30 0.0 0.0 0.0 0.39709054809950173 0.0 31 0.0 0.0 0.0 0.4276359748763865 0.0 32 0.0 0.0 0.0 0.4600904908268265 0.0 33 0.0 0.0 0.0 0.5154540768599302 0.0 34 0.0 0.0 0.0 0.5536358603310361 0.0 35 0.0 0.0 0.0 0.6872721024799069 0.0 36 0.0 0.0 0.0 0.8819991981825471 0.0 37 0.0 0.0 0.0 1.151180771653844 0.0 38 0.0 0.0 0.0 1.4948168228937975 0.0 39 0.0 0.0 0.0 1.9663618487619556 0.0 40 0.0 0.0 0.0 2.5066340848781046 0.0 41 0.0 0.0 0.0 3.1862698306637904 0.0 42 0.0 0.0 0.0 3.8239056146312596 0.0 43 0.0 0.0 0.0 4.482541379507837 0.0 44 0.0 0.0 0.0 5.0342681506653175 0.0 45 0.0 0.0 0.0 5.818903800996544 0.0 46 0.0 0.0 0.0 6.3820851071953575 0.0 47 0.0 0.0 0.0 7.090357190584372 0.0 48 0.0 0.0 0.0 7.603902178270747 0.0 49 0.0 0.0 0.0 8.298810637444875 0.0 50 0.0 0.0 0.0 8.980355472404115 0.0 51 0.0 0.0 0.0 9.518718619346709 0.0 52 0.0 0.0 0.0 10.104808995628186 0.0 53 0.0 0.0 0.0 10.780626563066761 0.0 54 0.0 0.0 0.0 11.359080582654016 0.0 55 0.0 0.0 0.0 11.912716442985051 0.0 56 0.0 0.0 0.0 12.349897863729215 0.0 57 0.0 0.0 0.0 12.844351959680036 0.0 58 0.0 0.0 0.0 13.359806036539966 0.0 59 0.0 0.0 0.0 13.875260113399897 0.0 60 0.0 0.0 0.0 14.331532425879614 0.0 61 0.0 0.0 0.0 14.776350203317998 0.0 62 0.0 0.0 0.0 15.236440694144823 0.0 63 0.0 0.0 0.0 15.665985758194765 0.0 64 0.0 0.0 0.0 16.137530784062925 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTC 15 0.0022137475 69.99427 12 TGAAGGT 15 0.0022137475 69.99427 28 GTCTCGA 15 0.0022137475 69.99427 60 TTGAGGC 15 0.0022137475 69.99427 11 TATTGAG 15 0.0022137475 69.99427 9 ACGTCTC 15 0.0022137475 69.99427 58 GCGGATG 15 0.0022137475 69.99427 36 GGCGCCA 15 0.0022137475 69.99427 15 CGCCATT 25 2.3709038E-4 55.99542 17 TGTTTTG 20 0.0069174552 52.4957 27 CTTCGGC 20 0.0069174552 52.4957 24 ATGATTC 20 0.0069174552 52.4957 40 AATTTAC 20 0.0069174552 52.4957 53 CAAGTGA 20 0.0069174552 52.4957 28 TTCAGCC 20 0.0069174552 52.4957 44 CGTCTCG 20 0.0069174552 52.4957 59 GCCATTG 20 0.0069174552 52.4957 18 AATATTG 20 0.0069174552 52.4957 7 AGCGGAT 20 0.0069174552 52.4957 35 CGGATGA 20 0.0069174552 52.4957 37 >>END_MODULE