FastQCFastQC Report
Wed 25 May 2016
SRR1780674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52381
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT13612.5982703652087586No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT5661.080544472232298Illumina PCR Primer Index 10 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1740.33218151619862163No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1460.27872701933907335No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG900.1718180256199767No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC660.12599988545464957Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAG252.8032719E-670.000019
ATAAAGA252.8032719E-670.000011
AGAATAT252.8032719E-670.000015
CAGAAGT150.00221302970.068
ATGATAA150.00221302970.039
GGCGCCA207.858253E-570.015
CTAATGT150.00221302970.03
CGCCATT207.858253E-570.017
TGAAGGT308.26937E-658.33333228
TGGCGTG308.26937E-658.33333223
TTGAGGC308.26937E-658.33333211
ATTGGCG308.26937E-658.33333221
GCGTGAA308.26937E-658.33333225
GAGGCGC308.26937E-658.33333213
AATTGTG252.3699446E-456.00000429
GAATTGT252.3699446E-456.00000428
AGAATTG252.3699446E-456.00000427
GTGAAGA252.3699446E-456.00000433
CTGCTTA200.006915214552.546
GATAATC200.006915214552.548
Produced by FastQC (version 0.11.5)