##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780674_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52381 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.87478284110651 34.0 34.0 34.0 31.0 34.0 2 33.36562875851931 34.0 34.0 34.0 33.0 34.0 3 33.500715908440085 34.0 34.0 34.0 34.0 34.0 4 33.54437677783929 34.0 34.0 34.0 34.0 34.0 5 33.559515854985584 34.0 34.0 34.0 34.0 34.0 6 37.32465970485482 38.0 38.0 38.0 37.0 38.0 7 36.996029094519 38.0 37.0 38.0 36.0 38.0 8 36.99381455107768 38.0 37.0 38.0 35.0 38.0 9 36.81747198411638 38.0 37.0 38.0 35.0 38.0 10-11 36.542763597487635 38.0 37.0 38.0 34.0 38.0 12-13 36.420371890571005 38.0 37.0 38.0 34.0 38.0 14-15 36.66610984899105 38.0 37.0 38.0 34.0 38.0 16-17 36.76668066665394 38.0 37.0 38.0 34.5 38.0 18-19 36.79508791355644 38.0 37.0 38.0 34.0 38.0 20-21 36.55440904144633 38.0 37.0 38.0 34.0 38.0 22-23 36.62843397415094 38.0 37.0 38.0 34.0 38.0 24-25 36.02222179798018 38.0 36.5 38.0 32.5 38.0 26-27 36.214552986770016 38.0 37.0 38.0 34.0 38.0 28-29 36.08843855596495 38.0 37.0 38.0 33.5 38.0 30-31 35.834338786964736 38.0 36.5 38.0 32.5 38.0 32-33 36.28569519482255 38.0 37.0 38.0 34.0 38.0 34-35 36.289188828010154 38.0 37.0 38.0 34.0 38.0 36-37 36.434317786983826 38.0 37.0 38.0 34.0 38.0 38-39 36.006758175674385 38.0 37.0 38.0 32.5 38.0 40-41 35.66869666482121 38.0 36.5 38.0 31.5 38.0 42-43 36.09646627593975 38.0 37.0 38.0 33.0 38.0 44-45 36.271014299077905 38.0 37.0 38.0 34.0 38.0 46-47 36.49337546056776 38.0 37.0 38.0 34.0 38.0 48-49 36.32045016322712 38.0 37.0 38.0 34.0 38.0 50-51 36.438632328516064 38.0 37.0 38.0 34.0 38.0 52-53 36.36688875737386 38.0 37.0 38.0 34.0 38.0 54-55 36.51161680762108 38.0 37.0 38.0 34.0 38.0 56-57 36.57899811000172 38.0 37.0 38.0 34.0 38.0 58-59 36.59839445600504 38.0 37.0 38.0 34.0 38.0 60-61 36.3333746969321 38.0 37.0 38.0 34.0 38.0 62-63 36.57144766232031 38.0 37.0 38.0 34.0 38.0 64-65 36.58464901395544 38.0 37.0 38.0 34.0 38.0 66-67 35.981663198488 38.0 37.0 38.0 33.0 38.0 68-69 35.337927874611026 38.0 36.0 38.0 29.0 38.0 70-71 35.23987705465722 38.0 36.0 38.0 29.0 38.0 72-73 34.80285790649281 38.0 36.0 38.0 26.0 38.0 74-75 35.317643802142 38.0 36.5 38.0 29.5 38.0 76 34.8062083579924 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1281595003657401 1101 2 0.33601405761453407 1101 3 0.07806921801903144 1101 4 -0.01774808542723605 1101 5 0.27133030361989796 1101 6 -1.5480510083515426 1101 7 0.4281969876521785 1101 8 0.08262919816532843 1101 9 0.2703406967597388 1101 10-11 0.050526355882801965 1101 12-13 0.7416374409685602 1101 14-15 0.35116605887829877 1101 16-17 -0.21974281978331334 1101 18-19 -0.3627055764457978 1101 20-21 0.3298879507814334 1101 22-23 0.582036985307461 1101 24-25 -1.3218102830867267 1101 26-27 -0.5944150247843041 1101 28-29 0.18079380531512612 1101 30-31 0.3436806836900175 1101 32-33 -0.20893468031238172 1101 34-35 -0.17258237631894957 1101 36-37 0.450023166709407 1101 38-39 0.35181101282015703 1101 40-41 1.1292417130831396 1101 42-43 0.672975533882088 1101 44-45 0.08284489890812097 1101 46-47 0.4447789333244643 1101 48-49 -1.2216343787730182 1101 50-51 -1.1148257129658248 1101 52-53 -1.962159781531554 1101 54-55 -0.8820060320844476 1101 56-57 -0.9941501686425838 1101 58-59 -1.5908181309557818 1101 60-61 -1.8417557866360639 1101 62-63 -0.5925831204813363 1101 64-65 -0.933679816123373 1101 66-67 -1.1476197028036097 1101 68-69 -1.321296180598459 1101 70-71 -1.3210309792680164 1101 72-73 -2.5654878238585255 1101 74-75 -1.6212229587623384 1101 76 -1.5292648208134167 1102 1 -0.38066106415038803 1102 2 0.16489510600163015 1102 3 0.32630510511580724 1102 4 0.4184918339276038 1102 5 0.012005706845705788 1102 6 0.5147010077774894 1102 7 0.016906665071530824 1102 8 0.48031064977823235 1102 9 0.5657035999855466 1102 10-11 0.8196795816892504 1102 12-13 0.7815819974201759 1102 14-15 -0.026858134670085576 1102 16-17 0.13686000279733435 1102 18-19 -0.5542378345103174 1102 20-21 0.6123980314265935 1102 22-23 0.6582718643397172 1102 24-25 -4.785771976635111 1102 26-27 -0.9510178473649447 1102 28-29 0.18495207144415815 1102 30-31 1.5452935869158253 1102 32-33 0.8050522148489136 1102 34-35 0.6841464543262106 1102 36-37 -2.085133083290593 1102 38-39 0.27255194023951645 1102 40-41 0.9925229630831396 1102 42-43 0.7644573887207997 1102 44-45 0.8804759472952242 1102 46-47 1.017485586550272 1102 48-49 -2.5132170400633385 1102 50-51 -0.4326180516755045 1102 52-53 -0.16465474120897738 1102 54-55 0.47396675017361645 1102 56-57 0.4288589039380568 1102 58-59 -0.07279897772997401 1102 60-61 -4.332557197926384 1102 62-63 -2.0074016688684395 1102 64-65 0.2724693774250113 1102 66-67 0.8590587246157497 1102 68-69 -0.07621049511458722 1102 70-71 -0.5421751324938242 1102 72-73 -5.1011481061165895 1102 74-75 -1.842115095859107 1102 76 -0.7195369982327691 1103 1 0.5639865422325911 1103 2 0.24850547834205372 1103 3 0.22374526469027245 1103 4 0.25043997222547887 1103 5 -0.08734048109755577 1103 6 0.10717662834486674 1103 7 0.6481123388303942 1103 8 0.4910597632534106 1103 9 0.12266281984370409 1103 10-11 -0.10075481547387 1103 12-13 -1.3976899440691852 1103 14-15 -0.943303170131081 1103 16-17 0.0851645240739316 1103 18-19 0.3433685484684048 1103 20-21 -0.21400932672943895 1103 22-23 0.3539999228503561 1103 24-25 -3.089683767415252 1103 26-27 -0.5243223686415419 1103 28-29 -0.5930565721728627 1103 30-31 0.014265218121501277 1103 32-33 0.5294261066928811 1103 34-35 -0.1437846183688265 1103 36-37 -1.0632747056310166 1103 38-39 -0.3706495225264419 1103 40-41 -0.025013606065797944 1103 42-43 -0.5183994375203369 1103 44-45 0.7107618515505436 1103 46-47 0.7944414819403463 1103 48-49 -2.3838398237512806 1103 50-51 -0.8551025374911063 1103 52-53 -0.4340197678047275 1103 54-55 -0.6185365742944668 1103 56-57 -0.35590949145201733 1103 58-59 -0.1028577099994763 1103 60-61 -4.176833572039861 1103 62-63 -1.517485888726597 1103 64-65 -0.4486077502345651 1103 66-67 -1.3807185360225418 1103 68-69 -0.5450436068167122 1103 70-71 -0.5386013293023382 1103 72-73 -5.384808345478291 1103 74-75 -2.2864025515328663 1103 76 -3.818329108161848 1104 1 0.0472903247384977 1104 2 0.2629853837794087 1104 3 0.14661760511580724 1104 4 -0.3705706660723962 1104 5 -0.1728901264876299 1104 6 -0.019153158889174904 1104 7 0.13496222062708796 1104 8 0.35010231644489664 1104 9 0.8122313777633252 1104 10-11 -0.6074037516440782 1104 12-13 0.231668802975733 1104 14-15 0.493975198663243 1104 16-17 0.3026586139084486 1104 18-19 0.8138177210452398 1104 20-21 -0.2582616907956279 1104 22-23 0.14308089211748864 1104 24-25 0.39044330114266756 1104 26-27 1.015127985968384 1104 28-29 1.258302765888601 1104 30-31 0.7397380313602682 1104 32-33 0.8046181870711351 1104 34-35 0.4962124265484391 1104 36-37 0.918773166709407 1104 38-39 1.3537151346839593 1104 40-41 1.4243806019720253 1104 42-43 0.4337282220541354 1104 44-45 0.5618995584063384 1104 46-47 0.6081973921058292 1104 48-49 -0.2120017622855599 1104 50-51 0.6255416705467169 1104 52-53 0.6482792865688012 1104 54-55 0.286900777951395 1104 56-57 0.2782512650491711 1104 58-59 -0.2503163388410883 1104 60-61 0.7407934965180587 1104 62-63 0.26516777557600335 1104 64-65 0.5380943774250113 1104 66-67 0.2292844190601926 1104 68-69 1.4033901993298556 1104 70-71 1.1336061175061758 1104 72-73 1.1822720327722962 1104 74-75 0.39356198747422155 1104 76 0.38202550176723094 1105 1 0.625313200555496 1105 2 -0.2590734397500043 1105 3 0.09024505609620093 1105 4 0.07591626203218027 1105 5 -0.48784110687978455 1105 6 -0.0024047928761063986 1105 7 0.4352073186663006 1105 8 -0.20300225871849875 1105 9 0.5552869333188823 1105 10-11 0.2794426535846739 1105 12-13 -0.4891236806844006 1105 14-15 0.26828075421880015 1105 16-17 0.2797827969149864 1105 18-19 0.45107262300602713 1105 20-21 0.37756347260306455 1105 22-23 0.21354657839200542 1105 24-25 0.25053317042371503 1105 26-27 0.04249726701414147 1105 28-29 0.4029106090258523 1105 30-31 1.029770711098827 1105 32-33 0.8987766838031632 1105 34-35 -0.6036650244319617 1105 36-37 0.44103623860483054 1105 38-39 -0.0651982640088491 1105 40-41 -0.2384053457403894 1105 42-43 0.42882626126981904 1105 44-45 0.5000123035043771 1105 46-47 0.9396924901450419 1105 48-49 -0.3774429387561469 1105 50-51 0.5491527816578241 1105 52-53 0.8856158878759857 1105 54-55 0.6561818237030295 1105 56-57 0.16223819315374755 1105 58-59 0.24396470691054617 1105 60-61 0.8235140847533557 1105 62-63 -0.26383549239784543 1105 64-65 0.7529636584707617 1105 66-67 0.6968089288641153 1105 68-69 0.16278562416645315 1105 70-71 0.7763757253493111 1105 72-73 0.6455073268899483 1105 74-75 -0.2820916072970121 1105 76 -0.16862809300400983 1106 1 -0.5443863339345612 1106 2 0.1641644405340017 1106 3 0.39931544684242937 1106 4 0.312432931049905 1106 5 0.22833889029894294 1106 6 0.3431070329573487 1106 7 -1.0694242701802708 1106 8 0.40375939078542444 1106 9 -1.2816435223166138 1106 10-11 -0.11175027442585872 1106 12-13 1.1916258373482336 1106 14-15 -0.43355777495785475 1106 16-17 -0.06744530295806328 1106 18-19 0.46689525901486206 1106 20-21 0.2146349918582473 1106 22-23 0.28144520067065315 1106 24-25 -0.21624634713870705 1106 26-27 0.8369205519156324 1106 28-29 0.37770724230746566 1106 30-31 0.28540150058488933 1106 32-33 0.3753366123309263 1106 34-35 0.6492150244700952 1106 36-37 0.9203469077165991 1106 38-39 0.3424991075057022 1106 40-41 0.7497453102054408 1106 42-43 0.5842078383610882 1106 44-45 0.7464269365038589 1106 46-47 0.6832623401473938 1106 48-49 1.0106701002244307 1106 50-51 0.09351729005111054 1106 52-53 0.4707712929636898 1106 54-55 -0.26425829479040885 1106 56-57 0.12217612516107579 1106 58-59 0.6656508873779359 1106 60-61 0.6198652391239747 1106 62-63 0.42279167645530435 1106 64-65 -1.3135243276109634 1106 66-67 0.015730262385531546 1106 68-69 1.3011220138782207 1106 70-71 -0.3553776954434653 1106 72-73 -0.025861460433134198 1106 74-75 0.09170347428477754 1106 76 0.7795075161557179 1107 1 0.6809354494550561 1107 2 -0.17210531916843763 1107 3 0.18828427178247154 1107 4 -0.020655700086003037 1107 5 -0.010049990433202538 1107 6 0.1657958207026624 1107 7 0.7516005426225547 1107 8 0.32374177222720846 1107 9 0.13351822583588557 1107 10-11 0.3488037313491148 1107 12-13 0.06663195490317264 1107 14-15 0.1583034299557653 1107 16-17 0.47386042796740213 1107 18-19 0.4895546824964896 1107 20-21 0.4373874021748918 1107 22-23 0.025804815019988325 1107 24-25 0.16049715601794645 1107 26-27 -0.4062155514465786 1107 28-29 0.7721916547774867 1107 30-31 0.41859290664371684 1107 32-33 0.4869452845768052 1107 34-35 -0.03957272084385721 1107 36-37 0.9287646633080513 1107 38-39 0.11654109840278437 1107 40-41 0.5595138219266786 1107 42-43 0.5340683581085557 1107 44-45 0.6980248418530408 1107 46-47 0.9635687072985704 1107 48-49 0.4460197796645531 1107 50-51 0.9777242102292547 1107 52-53 0.618375658450887 1107 54-55 0.4955175579967488 1107 56-57 0.410337432849623 1107 58-59 0.42457028247410733 1107 60-61 0.784231705134836 1107 62-63 0.679141698478503 1107 64-65 0.6613936971529029 1107 66-67 -0.1515971002141825 1107 68-69 1.4355614011439144 1107 70-71 0.5470840086626367 1107 72-73 1.1241654568085764 1107 74-75 0.21584089903885229 1107 76 1.0503928487060037 1108 1 0.11717803159429252 1108 2 0.5038246272782274 1108 3 0.1173622859668697 1108 4 0.37809954669356216 1108 5 0.14670207209393027 1108 6 -0.1694191163359875 1108 7 0.2793180125892647 1108 8 -0.20397569773950153 1108 9 0.06592523119122262 1108 10-11 0.45598277317861147 1108 12-13 1.1590476445832962 1108 14-15 0.7836471844788448 1108 16-17 0.06388792832925105 1108 18-19 0.28308486052514326 1108 20-21 0.7186147867457464 1108 22-23 0.2618013412900737 1108 24-25 0.9457772254925487 1108 26-27 -0.22290392892522704 1108 28-29 -0.17461685586080478 1108 30-31 0.5710028067739827 1108 32-33 0.7741069577567146 1108 34-35 0.6753643178013888 1108 36-37 1.0998458617448676 1108 38-39 1.4484994136437663 1108 40-41 1.0671849754944915 1108 42-43 0.46741616531654273 1108 44-45 0.7391306458767843 1108 46-47 0.6774202053445961 1108 48-49 1.0275076939792172 1108 50-51 0.7619187391046438 1108 52-53 0.2610156931881846 1108 54-55 0.2892648441452508 1108 56-57 0.3887713596118161 1108 58-59 0.28721321198634087 1108 60-61 0.6174926690948936 1108 62-63 0.514429004890431 1108 64-65 0.36344898735409004 1108 66-67 1.229210541991634 1108 68-69 1.2244599392825748 1108 70-71 -0.13079991086262055 1108 72-73 0.33504981055007477 1108 74-75 -0.2864025515328663 1108 76 0.8412453599232563 1109 1 -2.2445240960652804 1109 2 -0.10610445073595542 1109 3 -0.03866608282745432 1109 4 -0.40204229727807217 1109 5 -0.0731560839344354 1109 6 -0.651688619882087 1109 7 -0.358979859751166 1109 8 -0.28198988213666354 1109 9 -0.04754994611374741 1109 10-11 0.24676291502258607 1109 12-13 0.534934169405993 1109 14-15 0.6027961206490673 1109 16-17 0.47523544605974166 1109 18-19 0.33273025059606454 1109 20-21 0.4810261342634732 1109 22-23 0.08804247604184212 1109 24-25 -0.6145064624507128 1109 26-27 -0.12006704949260438 1109 28-29 -1.3093686289104483 1109 30-31 -0.22332343436077196 1109 32-33 0.025880007402108163 1109 34-35 -0.3530044765248519 1109 36-37 0.5785692660001871 1109 38-39 0.430768917189873 1109 40-41 -0.6562192798246613 1109 42-43 0.5737991440399526 1109 44-45 -0.13675587894591246 1109 46-47 0.07103722662119338 1109 48-49 0.08424528263169861 1109 50-51 -0.1919819701152221 1109 52-53 -0.8524594841167783 1109 54-55 -0.9802387019540433 1109 56-57 -0.2743492077640681 1109 58-59 -0.10994990858103648 1109 60-61 0.3728118180310602 1109 62-63 0.021521203471991157 1109 64-65 0.8067113987016086 1109 66-67 0.14055805972213165 1109 68-69 -0.38901523802238813 1109 70-71 0.542958954385611 1109 72-73 0.1967519382096512 1109 74-75 -1.2083883671357043 1109 76 0.011458125880707826 1110 1 -1.340718282460248 1110 2 -0.5869754925899215 1110 3 -0.06993169065884075 1110 4 -0.15666221536817204 1110 5 0.4025206716344414 1110 6 0.11846421200284141 1110 7 0.3831038481701228 1110 8 0.6640694525951361 1110 9 0.8487141633658268 1110 10-11 -0.7661478830994852 1110 12-13 0.2117646558708799 1110 14-15 0.9075608794144259 1110 16-17 0.5225334182902941 1110 18-19 -0.31920262324271675 1110 20-21 0.9001401088913852 1110 22-23 -0.6914750546743633 1110 24-25 -1.2125985611421513 1110 26-27 0.9080270470012479 1110 28-29 -0.11747970672485053 1110 30-31 -0.24872049759121495 1110 32-33 0.4393501902010257 1110 34-35 0.5983740071431143 1110 36-37 1.0538964061460305 1110 38-39 0.7134080141831802 1110 40-41 -1.2651244840999638 1110 42-43 -0.03340792818060834 1110 44-45 0.6933060529290316 1110 46-47 0.7397016957052003 1110 48-49 1.1484246852887736 1110 50-51 -0.47080026998536084 1110 52-53 0.8414522130163746 1110 54-55 -0.05073615475595972 1110 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36-37 -0.07911217163646 2116 38-39 -4.238990587955971 2116 40-41 -1.6094800914281322 2116 42-43 -1.435319396993485 2116 44-45 -0.2867585451859753 2116 46-47 -0.48236756194597064 2116 48-49 0.04358512159079453 2116 50-51 -1.1300452133296375 2116 52-53 0.010069177963956122 2116 54-55 -0.8931326389241292 2116 56-57 -0.19188240245291865 2116 58-59 -2.7284909420156893 2116 60-61 -1.7146941810090937 2116 62-63 -0.049107495936112855 2116 64-65 -2.210965772950928 2116 66-67 -0.783534085534626 2116 68-69 -1.894907628573236 2116 70-71 0.43036363630317 2116 72-73 0.08191697846987722 2116 74-75 -0.4726660827012168 2116 76 -1.2213579568793875 2117 1 0.8514569914051648 2117 2 0.5731705689645921 2117 3 0.22532130881950962 2117 4 -0.2839965919983243 2117 5 0.29516542906792864 2117 6 0.45075424851822987 2117 7 0.7650548132196775 2117 8 0.495472686815269 2117 9 -0.3113797333477848 2117 10-11 -0.14212597386630677 2117 12-13 -1.5180997155427889 2117 14-15 0.31272519866325155 2117 16-17 -0.4640080527582171 2117 18-19 -1.1158119085843907 2117 20-21 0.05076608698215068 2117 22-23 0.5632197810063886 2117 24-25 -0.8806215136721462 2117 26-27 -1.4529275695871675 2117 28-29 -1.0204009378151042 2117 30-31 0.27399729061952627 2117 32-33 -0.887279961077013 2117 34-35 0.8353327969188058 2117 36-37 -0.547199055512813 2117 38-39 0.5284836532024784 2117 40-41 -0.23927680543538088 2117 42-43 0.12446896279487163 2117 44-45 -0.0827763675195925 2117 46-47 -0.7378674227089874 2117 48-49 -0.09857583635962897 2117 50-51 0.041745374250425016 2117 52-53 0.8644829902725064 2117 54-55 0.46467855572917216 2117 56-57 0.2578808946787987 2117 58-59 -0.41790893143367924 2117 60-61 -0.5344379849634251 2117 62-63 0.0017418496500738456 2117 64-65 -1.1239426596120268 2117 66-67 -0.10798409945832077 2117 68-69 0.5464457548854185 2117 70-71 -0.3763475861975252 2117 72-73 1.0943090698093343 2117 74-75 0.7667101356223753 2117 76 1.0209143906561167 2118 1 -0.02909856415038803 2118 2 0.46547076389637 2118 3 -0.7218034475157751 2118 4 0.020876702348658682 2118 5 0.32162741737202083 2118 6 0.36132929725117435 2118 7 0.7800645598083733 2118 8 0.61764617609402 2118 9 -1.8767306105407684 2118 10-11 -0.43525462883706467 2118 12-13 -1.3419788578429817 2118 14-15 0.6195234442772843 2118 16-17 -2.097926181413193 2118 18-19 -0.5377904660892625 2118 20-21 -0.44886841594182414 2118 22-23 0.6761583774976074 2118 24-25 1.0254944707333138 2118 26-27 -2.2524158736807394 2118 28-29 -0.11706273118741706 2118 30-31 -0.869180097294695 2118 32-33 -0.25477508778266156 2118 34-35 -0.10594400620010447 2118 36-37 -3.007213675395853 2118 38-39 -0.4673740466025862 2118 40-41 0.3167417130831396 2118 42-43 0.7155264676681696 2118 44-45 -1.5212510263889811 2118 46-47 0.7187602576029022 2118 48-49 0.5299573020419217 2118 50-51 -0.5276016043070797 2118 52-53 -1.688709017524772 2118 54-55 -0.7145612103526986 2118 56-57 0.1704296276222621 2118 58-59 -0.16223154351944657 2118 60-61 -0.3237167374000691 2118 62-63 -0.5049345636052749 2118 64-65 0.5940154300565865 2118 66-67 0.11214260619469485 2118 68-69 -1.708817403009327 2118 70-71 -0.5851438824938242 2118 72-73 0.42496209125183526 2118 74-75 -0.18319239849068225 2118 76 0.480709712293546 2119 1 0.8070125469607206 2119 2 -0.6490516532576294 2119 3 -0.19690091340271465 2119 4 -0.36547807347980665 2119 5 -0.34187160796911087 2119 6 -0.5492457514817701 2119 7 -0.6423525941877273 2119 8 0.32510231644489807 2119 9 -0.18545380742185813 2119 10-11 -0.2902741220144591 2119 12-13 -0.7143960118390851 2119 14-15 -0.1835710976330489 2119 16-17 -0.33808212683229044 2119 18-19 -0.2898859826584612 2119 20-21 -1.1010857648697083 2119 22-23 -0.24048392269731522 2119 24-25 0.8193784863278566 2119 26-27 0.4729983563387563 2119 28-29 0.38700646959230767 2119 30-31 0.6406639572861934 2119 32-33 0.04605337225632411 2119 34-35 0.5131105746965829 2119 36-37 -0.38423609254984825 2119 38-39 -0.741886717167894 2119 40-41 -0.5689064350650099 2119 42-43 0.6170615553874654 2119 44-45 0.23944585470263036 2119 46-47 0.2547251698836064 2119 48-49 -0.8207980585818575 2119 50-51 0.43063426313931075 2119 52-53 0.745964471753986 2119 54-55 0.33504892609954595 2119 56-57 -0.4384154016174975 2119 58-59 -0.17346448698923211 2119 60-61 -0.04925279977823749 2119 62-63 0.1795196274278581 2119 64-65 0.09457585890649511 2119 66-67 0.44016404868982306 2119 68-69 0.5538531622928176 2119 70-71 0.3125413026913577 2119 72-73 0.9424572179574895 2119 74-75 -0.2888454199331818 2119 76 0.45795142769315333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 2.0 23 6.0 24 17.0 25 39.0 26 72.0 27 138.0 28 240.0 29 417.0 30 698.0 31 1030.0 32 1750.0 33 2631.0 34 4224.0 35 6377.0 36 11671.0 37 23066.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.69196846184685 24.327523338615148 11.76762566579485 29.21288253374315 2 16.689257555220404 18.31580153108952 41.23823523796796 23.756705675722113 3 16.082167198029822 26.139248964319123 31.79397109638991 25.98461274126114 4 11.538534964968214 21.158435310513354 39.10005536358604 28.202974360932398 5 10.923808251083408 37.94123823523797 38.97023729978427 12.164716213894351 6 25.644794868368304 42.38941600962181 17.846165594394915 14.119623527614975 7 24.697886638284874 30.364063305396993 23.739523873160113 21.198526183158016 8 21.771252935224606 38.23332887879193 20.24589068555392 19.749527500429544 9 22.481434107787173 13.7855329222428 22.290525190431644 41.44250777953838 10-11 23.365342395143276 26.459021400889636 28.934155514404075 21.24148068956301 12-13 23.1209789809282 22.55588858555583 27.332429697791184 26.990702735724785 14-15 21.072526297703366 24.207250720681163 23.275615203986177 31.44460777762929 16-17 22.79261564307669 28.403428724155706 24.704568450392316 24.09938718237529 18-19 22.947251866134664 26.86565739485691 24.992840915599167 25.194249823409255 20-21 20.955117313529716 26.844657413947804 24.908840991962737 27.291384280559743 22-23 21.563162215307077 28.397701456635037 26.368339665145758 23.670796662912124 24-25 22.56352494225005 29.02674633932151 25.61234035241786 22.797388366010576 26-27 22.798342910597356 29.466791393826004 25.353658769401115 22.38120692617552 28-29 21.26152612588534 28.25261067944484 27.389702372997842 23.09616082167198 30-31 23.044615413985987 27.554838586510378 27.80874744659323 21.591798552910408 32-33 22.186479830472884 28.344246959775493 27.59397491456826 21.875298295183367 34-35 21.239571600389453 28.232565243122504 29.032473606842174 21.495389549645864 36-37 21.15557167675302 27.78488383192379 28.269792482006835 22.789752009316356 38-39 20.792844733777514 26.25474885931922 28.407246902502813 24.545159504400452 40-41 20.441572325843342 25.656249403409632 29.344609686718464 24.557568584028562 42-43 22.99879727382066 25.592294916095533 29.04201905270995 22.366888757373857 44-45 19.830663790305643 26.70529390427827 29.87438193238006 23.589660373036025 46-47 22.480479563200397 25.06347721502071 28.192474370477843 24.263568851301045 48-49 21.546934957331857 24.897386456921403 28.329928790973824 25.225749794772913 50-51 19.401118726255703 25.550294954277312 29.809472900479182 25.239113418987802 52-53 18.877073748114775 25.50065863576488 32.486015921803705 23.13625169431664 54-55 18.269028846337413 25.921612798533822 30.552108588992194 25.257249766136574 56-57 21.224298887001012 25.40043145415322 28.15333804241996 25.221931616425803 58-59 18.595483095015368 26.626066703575724 30.71629025791795 24.06215994349096 60-61 20.839617418529617 28.617246711593896 27.00692999370001 23.536205876176478 62-63 18.078119928981884 31.22888070101754 26.18220347072412 24.510795899276456 64-65 18.76634657604857 31.87606193085279 24.956568221301616 24.401023271797026 66-67 18.857028311792444 31.48088047192684 25.019568164028943 24.64252305225177 68-69 18.812164716213893 31.235562513124986 24.957522765888395 24.994750004772722 70-71 19.409709627536703 30.71629025791795 24.414386896011912 25.459613218533438 72-73 19.582482197743456 29.834291059735403 24.48025047249957 26.10297627002157 74-75 19.70657299402455 30.280063381760563 24.16334166968939 25.8500219545255 76 20.2382543288597 29.835245604322182 24.72079570836754 25.205704358450582 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 3.0 21 5.0 22 9.5 23 25.0 24 31.5 25 27.0 26 52.0 27 118.0 28 186.0 29 213.0 30 274.5 31 376.5 32 588.0 33 759.0 34 882.5 35 1149.5 36 1427.5 37 1562.0 38 2018.5 39 2880.5 40 3532.5 41 3843.0 42 3907.0 43 3931.0 44 4014.5 45 4167.0 46 4260.0 47 4074.0 48 3696.5 49 3333.5 50 3162.0 51 2905.0 52 2196.0 53 1675.0 54 1606.0 55 1507.0 56 1136.5 57 765.5 58 666.0 59 550.0 60 364.0 61 226.5 62 159.0 63 126.0 64 93.0 65 81.5 66 57.0 67 44.0 68 38.0 69 22.0 70 10.5 71 9.0 72 6.0 73 2.0 74 2.0 75 3.0 76 1.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.494148641683054 #Duplication Level Percentage of deduplicated Percentage of total 1 54.92257794517682 20.04352723315706 2 15.306549487340448 11.171989843645596 3 9.526051475204017 10.429354155132586 4 5.848503871102741 8.537446784139286 5 3.724628583385645 6.796357457856857 6 2.631303620004185 5.761631125789886 7 1.935551370579619 4.944540959508219 8 1.281648880518937 3.7418147801683816 9 0.973006905210295 3.195815276531567 >10 3.8135593220338984 20.692617552165864 >50 0.015693659761456372 0.39518145892594647 >100 0.010462439840970915 0.610908535537695 >500 0.0052312199204854574 1.080544472232298 >1k 0.0052312199204854574 2.5982703652087586 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 1361 2.5982703652087586 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 566 1.080544472232298 Illumina PCR Primer Index 10 (95% over 23bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 174 0.33218151619862163 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 146 0.27872701933907335 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 90 0.1718180256199767 No Hit CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 66 0.12599988545464957 Illumina PCR Primer Index 10 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.019090891735552968 0.0 2 0.0 0.0 0.0 0.02863633760332945 0.0 3 0.0 0.0 0.0 0.02863633760332945 0.0 4 0.0 0.0 0.0 0.04772722933888242 0.0 5 0.0 0.0 0.0 0.04772722933888242 0.0 6 0.0 0.0 0.0 0.04772722933888242 0.0 7 0.0 0.0 0.0 0.05345449685954831 0.0 8 0.0 0.0 0.0 0.05345449685954831 0.0 9 0.0 0.0 0.0 0.09354536950420954 0.0 10 0.0 0.0 0.0 0.12599988545464957 0.0 11 0.0 0.0 0.0 0.13172715297531548 0.0 12 0.0 0.0 0.0 0.14890895553731315 0.0 13 0.0 0.0 0.0 0.15845440140508962 0.0 14 0.0 0.0 0.0 0.16609075809931081 0.0 15 0.0 0.0 0.0 0.1813634714877532 0.0 16 0.0 0.0 0.0 0.18709073900841908 0.0 17 0.0 0.0 0.0 0.19854527404975086 0.0 18 0.0 0.0 0.0 0.22336343330596972 0.0 19 0.0 0.0 0.0 0.2348179683473015 0.0 20 0.0 0.0 0.0 0.2405452358679674 0.0 21 0.0 0.0 0.0 0.25963612760352034 0.0 22 0.0 0.0 0.0 0.27681793016551803 0.0 23 0.0 0.0 0.0 0.2901815543804051 0.0 24 0.0 0.0 0.0 0.295908821901071 0.0 25 0.0 0.0 0.0 0.31118153528951337 0.0 26 0.0 0.0 0.0 0.32454515950440044 0.0 27 0.0 0.0 0.0 0.3379087837192875 0.0 28 0.0 0.0 0.0 0.3779996563639488 0.0 29 0.0 0.0 0.0 0.39709054809950173 0.0 30 0.0 0.0 0.0 0.4199996181821653 0.0 31 0.0 0.0 0.0 0.44863595578549476 0.0 32 0.0 0.0 0.0 0.4829995609094901 0.0 33 0.0 0.0 0.0 0.5307267902483725 0.0 34 0.0 0.0 0.0 0.5708176628930337 0.0 35 0.0 0.0 0.0 0.7101811725625704 0.0 36 0.0 0.0 0.0 0.9182718924800978 0.0 37 0.0 0.0 0.0 1.202726179339837 0.0 38 0.0 0.0 0.0 1.5788167465302305 0.0 39 0.0 0.0 0.0 2.0694526641339417 0.0 40 0.0 0.0 0.0 2.6402703270269754 0.0 41 0.0 0.0 0.0 3.350451499589546 0.0 42 0.0 0.0 0.0 4.012905442813234 0.0 43 0.0 0.0 0.0 4.705904812813807 0.0 44 0.0 0.0 0.0 5.282449743227506 0.0 45 0.0 0.0 0.0 6.114812622897616 0.0 46 0.0 0.0 0.0 6.723812069261755 0.0 47 0.0 0.0 0.0 7.4607204902541 0.0 48 0.0 0.0 0.0 7.989538191328917 0.0 49 0.0 0.0 0.0 8.734082969015482 0.0 50 0.0 0.0 0.0 9.467173211660716 0.0 51 0.0 0.0 0.0 10.060899944636414 0.0 52 0.0 0.0 0.0 10.696626639430328 0.0 53 0.0 0.0 0.0 11.399171455298678 0.0 54 0.0 0.0 0.0 12.025352704224815 0.0 55 0.0 0.0 0.0 12.634352150588954 0.0 56 0.0 0.0 0.0 13.09444264141578 0.0 57 0.0 0.0 0.0 13.630896699184818 0.0 58 0.0 0.0 0.0 14.146350776044748 0.0 59 0.0 0.0 0.0 14.692350279681564 0.0 60 0.0 0.0 0.0 15.188713464805941 0.0 61 0.0 0.0 0.0 15.6602584906741 0.0 62 0.0 0.0 0.0 16.141348962410035 0.0 63 0.0 0.0 0.0 16.61289398827819 0.0 64 0.0 0.0 0.0 17.120711708443903 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTGAG 25 2.8032719E-6 70.00001 9 ATAAAGA 25 2.8032719E-6 70.00001 1 AGAATAT 25 2.8032719E-6 70.00001 5 CAGAAGT 15 0.002213029 70.0 68 ATGATAA 15 0.002213029 70.0 39 GGCGCCA 20 7.858253E-5 70.0 15 CTAATGT 15 0.002213029 70.0 3 CGCCATT 20 7.858253E-5 70.0 17 TGAAGGT 30 8.26937E-6 58.333332 28 TGGCGTG 30 8.26937E-6 58.333332 23 TTGAGGC 30 8.26937E-6 58.333332 11 ATTGGCG 30 8.26937E-6 58.333332 21 GCGTGAA 30 8.26937E-6 58.333332 25 GAGGCGC 30 8.26937E-6 58.333332 13 AATTGTG 25 2.3699446E-4 56.000004 29 GAATTGT 25 2.3699446E-4 56.000004 28 AGAATTG 25 2.3699446E-4 56.000004 27 GTGAAGA 25 2.3699446E-4 56.000004 33 CTGCTTA 20 0.0069152145 52.5 46 GATAATC 20 0.0069152145 52.5 48 >>END_MODULE