##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780672_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75100 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.379800266311584 34.0 31.0 34.0 12.0 34.0 2 31.42030625832224 34.0 32.0 34.0 23.0 34.0 3 31.60451398135819 34.0 34.0 34.0 23.0 34.0 4 32.048348868175765 34.0 34.0 34.0 27.0 34.0 5 31.969347536617843 34.0 34.0 34.0 27.0 34.0 6 34.48219707057257 38.0 35.0 38.0 25.0 38.0 7 33.955139813581894 37.0 34.0 38.0 23.0 38.0 8 32.626258322237014 37.0 31.0 38.0 11.0 38.0 9 33.51794940079893 37.0 34.0 38.0 21.0 38.0 10-11 34.043968042609855 37.0 34.0 38.0 23.5 38.0 12-13 34.42089880159787 38.0 35.0 38.0 24.5 38.0 14-15 34.05334221038615 37.0 34.0 38.0 23.0 38.0 16-17 33.95160452729694 37.0 34.0 38.0 23.0 38.0 18-19 33.916264980026625 37.0 34.0 38.0 22.5 38.0 20-21 33.99963382157124 37.0 34.0 38.0 23.0 38.0 22-23 34.05890146471371 37.0 34.0 38.0 23.5 38.0 24-25 34.47772303595207 38.0 35.0 38.0 25.0 38.0 26-27 34.55727696404793 38.0 35.5 38.0 25.0 38.0 28-29 34.67296937416778 38.0 36.0 38.0 26.0 38.0 30-31 34.511438082556595 38.0 35.5 38.0 25.0 38.0 32-33 34.242410119840216 38.0 34.0 38.0 23.5 38.0 34-35 34.11221704394141 37.0 34.0 38.0 23.5 38.0 36-37 34.111344873502 37.0 34.0 38.0 23.5 38.0 38-39 34.69922769640479 38.0 36.0 38.0 26.0 38.0 40-41 34.152676431424766 37.5 34.5 38.0 23.5 38.0 42-43 34.46951398135819 38.0 35.0 38.0 24.5 38.0 44-45 33.48883488681757 37.0 34.0 38.0 22.0 38.0 46-47 34.23368841544607 37.0 34.0 38.0 24.0 38.0 48-49 33.98374167776298 37.0 34.0 38.0 23.5 38.0 50-51 33.91146471371505 37.0 34.0 38.0 23.0 38.0 52-53 33.961857523302264 37.0 34.0 38.0 23.5 38.0 54-55 33.654720372836216 37.0 34.0 38.0 22.5 38.0 56-57 33.76612516644474 37.0 34.0 38.0 23.0 38.0 58-59 33.633442077230356 37.0 33.5 38.0 22.5 38.0 60-61 33.67352197070572 37.0 34.0 38.0 22.5 38.0 62-63 33.72624500665779 37.0 34.0 38.0 23.0 38.0 64-65 33.780399467376824 37.0 34.0 38.0 23.0 38.0 66-67 33.67039946737683 37.0 34.0 38.0 22.5 38.0 68-69 33.27349533954727 37.0 32.5 38.0 21.5 38.0 70-71 33.130599201065245 37.0 32.5 38.0 20.5 38.0 72-73 33.25261651131824 37.0 33.0 38.0 21.5 38.0 74-75 32.85717709720373 37.0 31.5 38.0 20.0 38.0 76 32.00595206391478 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.6121950926348063 1101 2 -0.031276520373872074 1101 3 0.30890604508861585 1101 4 -2.5032333733186434 1101 5 -0.8928158420554091 1101 6 -2.7924189404471313 1101 7 -1.1480985151552545 1101 8 -0.19703085868835046 1101 9 0.4266795999953388 1101 10-11 -0.668703416840458 1101 12-13 -0.541787912673172 1101 14-15 0.16676091420100647 1101 16-17 -0.8929306897554454 1101 18-19 0.8442942178817887 1101 20-21 -0.07221784170999257 1101 22-23 0.25255917305529607 1101 24-25 -0.8420775232836775 1101 26-27 -0.4286333389147643 1101 28-29 0.3970760558768802 1101 30-31 -0.6253074583204992 1101 32-33 0.781101001863945 1101 34-35 0.03076444656280586 1101 36-37 0.4878905571323813 1101 38-39 -0.44686444417100546 1101 40-41 0.8969378684797888 1101 42-43 0.41172561377322126 1101 44-45 0.45867699275788 1101 46-47 0.3759832832326069 1101 48-49 -0.8544289726504815 1101 50-51 -1.5396930177757184 1101 52-53 0.41230822170108894 1101 54-55 -0.6137714979063631 1101 56-57 0.1763282314544341 1101 58-59 -0.008613623809580417 1101 60-61 0.6664736070063242 1101 62-63 -1.0473183782038475 1101 64-65 0.019502867831072024 1101 66-67 -0.4094389410356598 1101 68-69 0.4197379000191006 1101 70-71 1.202703388742762 1101 72-73 -0.15770765912983364 1101 74-75 -0.739995469990518 1101 76 -0.7336467769755401 1102 1 0.7283000444120553 1102 2 -0.47106431878236066 1102 3 0.4561208992000232 1102 4 0.2292157867167255 1102 5 0.15767045325847207 1102 6 1.3571831815687858 1102 7 1.6916892832532326 1102 8 2.032765781453122 1102 9 0.8630191225417576 1102 10-11 -0.511143735142852 1102 12-13 -4.779630529826136 1102 14-15 -1.2146714465417006 1102 16-17 -1.9100394430710992 1102 18-19 -0.0973945531173328 1102 20-21 -0.18689511845622775 1102 22-23 -0.9995186341949207 1102 24-25 0.45580046080119985 1102 26-27 -4.921515743866138 1102 28-29 -0.34280015986140455 1102 30-31 0.3552849377891434 1102 32-33 -7.463903419002541 1102 34-35 -0.3728606639588605 1102 36-37 0.4959807428087757 1102 38-39 0.6228084117087462 1102 40-41 0.7615709365611352 1102 42-43 0.558100503251552 1102 44-45 -5.759758020504719 1102 46-47 0.06289751842270874 1102 48-49 0.3793906559967368 1102 50-51 -6.461487889570591 1102 52-53 -2.010945536035429 1102 54-55 0.872346981315566 1102 56-57 -0.7594365784483088 1102 58-59 0.8815720525829889 1102 60-61 0.630841423098282 1102 62-63 -4.976938183685718 1102 64-65 -0.5242637104182606 1102 66-67 0.658509776913057 1102 68-69 -7.536628147726255 1102 70-71 0.229759091660533 1102 72-73 1.0612136494466498 1102 74-75 -6.269571066807494 1102 76 -1.5429305966041866 1103 1 0.1469480630600728 1103 2 -0.3722543568145049 1103 3 -0.5387263588051283 1103 4 -0.7725508676815096 1103 5 -0.18272949766779334 1103 6 -1.8245368527828276 1103 7 0.9967070724815486 1103 8 1.2783307428075545 1103 9 0.3013702451034064 1103 10-11 0.1735943338057524 1103 12-13 -2.173201870765901 1103 14-15 -0.8362638891867746 1103 16-17 -0.7153884799990848 1103 18-19 -0.3098470129382136 1103 20-21 -0.9251848988512776 1103 22-23 -0.5620753756990311 1103 24-25 0.6410660723826069 1103 26-27 -0.3808826927067628 1103 28-29 0.4435205369856092 1103 30-31 0.1355818338755128 1103 32-33 -5.115038249084744 1103 34-35 -0.6271852867045382 1103 36-37 -0.1949038112336794 1103 38-39 0.3879537926436001 1103 40-41 0.3443337877450361 1103 42-43 0.5516227704580317 1103 44-45 -5.711935667419208 1103 46-47 -0.16988373541118307 1103 48-49 -0.03628840178757997 1103 50-51 -2.1933356519099334E-4 1103 52-53 0.24935381373760634 1103 54-55 0.43800763644990326 1103 56-57 0.9085918843169978 1103 58-59 0.31663523501459423 1103 60-61 0.7059610403231602 1103 62-63 0.22719627834347733 1103 64-65 0.8413969447160738 1103 66-67 -0.669805767191967 1103 68-69 -2.065041839297841 1103 70-71 0.8125587481444043 1103 72-73 1.2648573741429985 1103 74-75 -0.4510580009260039 1103 76 0.24713197240575369 1104 1 -1.134520468408457 1104 2 0.36323055301251017 1104 3 0.4176593607384831 1104 4 0.7859465559474934 1104 5 0.15222173530975525 1104 6 0.08667036105596537 1104 7 1.8900867191506663 1104 8 1.8106503968377368 1104 9 0.1370575840802175 1104 10-11 -0.1814962992454099 1104 12-13 -0.2251433503389535 1104 14-15 0.5106490662788161 1104 16-17 -0.23391764819930216 1104 18-19 0.9181503186775402 1104 20-21 1.4473997533386367 1104 22-23 0.7719557247794384 1104 24-25 0.8723068710576101 1104 26-27 1.0140611792107919 1104 28-29 0.2705011221898772 1104 30-31 -0.6690740365698318 1104 32-33 -0.15076239336151787 1104 34-35 0.014158566810372974 1104 36-37 0.4725833069113392 1104 38-39 -0.12671081906048443 1104 40-41 1.2285581160483119 1104 42-43 0.7773312724823214 1104 44-45 -3.658475969222664 1104 46-47 0.33196803124321406 1104 48-49 0.9510252713813543 1104 50-51 1.4434800591473618 1104 52-53 -0.4639904078303019 1104 54-55 -0.009383787915204778 1104 56-57 1.06011470360297 1104 58-59 0.8427900013009406 1104 60-61 0.4068029615598121 1104 62-63 1.0368438675963318 1104 64-65 -0.5242637104182606 1104 66-67 0.10818926409254459 1104 68-69 -3.215955070803183 1104 70-71 -0.4527729596215124 1104 72-73 1.0381367263697214 1104 74-75 -0.9277441437305711 1104 76 0.7330309418573506 1105 1 -0.7779170261885753 1105 2 0.14916750418918667 1105 3 -0.6633030558403696 1105 4 0.42528182113892044 1105 5 0.1976423535043459 1105 6 0.1192485134971335 1105 7 1.4842428484095365 1105 8 0.9759340287309541 1105 9 0.9669882128122183 1105 10-11 1.0644094787665352 1105 12-13 -0.9652645305286285 1105 14-15 -0.11972940228458384 1105 16-17 1.0770346700304145 1105 18-19 1.1228723945468744 1105 20-21 0.20277822190204375 1105 22-23 0.578177185966652 1105 24-25 0.5635278931861691 1105 26-27 0.011887838852516097 1105 28-29 -0.2967516877855374 1105 30-31 0.18893439335640494 1105 32-33 0.5507435778362364 1105 34-35 1.1673571091356294 1105 36-37 1.296542737891322 1105 38-39 0.8126222508376486 1105 40-41 0.5421118568280789 1105 42-43 0.7617885959807396 1105 44-45 1.8739265597552048 1105 46-47 0.8285104443096074 1105 48-49 0.5750703187996891 1105 50-51 0.16464488176765713 1105 52-53 -0.7426280088137887 1105 54-55 0.025104269689286696 1105 56-57 0.12928839520817093 1105 58-59 0.0727908794182568 1105 60-61 0.30527064684257255 1105 62-63 0.4460684900058922 1105 64-65 0.2800917514714385 1105 66-67 0.4547163101058871 1105 68-69 1.6148646517117484 1105 70-71 1.3896818173366867 1105 72-73 1.0825694625832867 1105 74-75 -1.0697466902707902 1105 76 -0.997935865308083 1106 1 0.3827579384638575 1106 2 0.5910468357145788 1106 3 -0.6700017091560895 1106 4 -0.4577814799759743 1106 5 -0.25867732755631323 1106 6 -0.6297490063786313 1106 7 0.8387151845938519 1106 8 0.6218325522457775 1106 9 1.3261194739669833 1106 10-11 0.34970489169563024 1106 12-13 0.6230116672323049 1106 14-15 0.9373134551893969 1106 16-17 0.7528917036163989 1106 18-19 0.23625115819335463 1106 20-21 -0.36187884876991205 1106 22-23 0.05336435422106689 1106 24-25 0.2714063439197716 1106 26-27 -0.0723552596802648 1106 28-29 -1.6648878860100425 1106 30-31 -0.21680929623402534 1106 32-33 0.505939090433877 1106 34-35 0.6357297304183405 1106 36-37 0.18858779734475917 1106 38-39 0.4838783062811842 1106 40-41 0.3393790844200453 1106 42-43 0.48242693823788585 1106 44-45 1.5593100518628447 1106 46-47 1.2875883943826167 1106 48-49 0.9019599648604668 1106 50-51 1.065636931462862 1106 52-53 0.5513478389482955 1106 54-55 0.0055761886564482666 1106 56-57 0.8588505489758731 1106 58-59 0.06426110633803717 1106 60-61 1.2252576667531017 1106 62-63 1.3078073584202272 1106 64-65 1.0333309791561263 1106 66-67 1.570679502280754 1106 68-69 1.5457224965533172 1106 70-71 0.7375945725924566 1106 72-73 0.7222444967719142 1106 74-75 -1.785111871180785 1106 76 -0.5203612877404602 1107 1 -0.7361688200568075 1107 2 0.3565618185211967 1107 3 0.45931125722908916 1107 4 0.2811831741679818 1107 5 0.529902522264738 1107 6 0.31717372865610827 1107 7 0.1138327312031322 1107 8 1.4314187413964063 1107 9 -0.8533934970966683 1107 10-11 -0.027461825654484073 1107 12-13 0.2642664334256608 1107 14-15 -0.2314858149689698 1107 16-17 0.6041162936652853 1107 18-19 1.0362932644414613 1107 20-21 0.6486360170748995 1107 22-23 -0.7732631692297787 1107 24-25 -0.23121360040641292 1107 26-27 0.42931729406367936 1107 28-29 -0.36106791361302726 1107 30-31 0.3196710892720631 1107 32-33 1.3893164793786426 1107 34-35 0.5885005554668723 1107 36-37 0.10602446252990916 1107 38-39 1.1755202160334548 1107 40-41 0.8265951596498766 1107 42-43 0.942964026824761 1107 44-45 0.2030093196819749 1107 46-47 0.2652252981698382 1107 48-49 0.567905367092095 1107 50-51 1.189569655035342 1107 52-53 1.330021024285962 1107 54-55 0.9286036278947947 1107 56-57 1.0162694359673807 1107 58-59 0.7335867045976485 1107 60-61 -0.22123230962061058 1107 62-63 0.2122027828746056 1107 64-65 0.4794583739489795 1107 66-67 1.2883337164144208 1107 68-69 0.8640808207246451 1107 70-71 0.6213696316581689 1107 72-73 0.8513235395565388 1107 74-75 0.3978163844509268 1107 76 -0.5756140421908569 1108 1 -0.6274172113052003 1108 2 0.0883258406077978 1108 3 0.13214307522219926 1108 4 -0.6607357032347672 1108 5 0.201468097056118 1108 6 -0.36464634026073384 1108 7 1.362929754493699 1108 8 1.1864733351606773 1108 9 -0.845920586397952 1108 10-11 0.47163519763608974 1108 12-13 0.3716168714212671 1108 14-15 0.866201734331483 1108 16-17 -0.4041142871459442 1108 18-19 -0.05085955658233132 1108 20-21 0.4037840222229008 1108 22-23 -0.2860397707160587 1108 24-25 -0.6957587907580489 1108 26-27 -0.8805594029097961 1108 28-29 0.7468741298128876 1108 30-31 0.34634522884942953 1108 32-33 0.966484661385536 1108 34-35 0.5093292182310236 1108 36-37 0.4655233623513979 1108 38-39 1.143147982048319 1108 40-41 0.5450428700330647 1108 42-43 0.9150651602162085 1108 44-45 0.7630417022950056 1108 46-47 0.8616154671406591 1108 48-49 0.41778289438897787 1108 50-51 0.9985952705125669 1108 52-53 0.5799068548169615 1108 54-55 0.844523003491588 1108 56-57 1.2612148422031098 1108 58-59 -0.607790386779449 1108 60-61 0.9218324140892733 1108 62-63 0.5479751912276569 1108 64-65 0.9033177171631692 1108 66-67 0.47344394184722205 1108 68-69 1.5906760795779675 1108 70-71 2.337139549040991 1108 72-73 1.1975268857598849 1108 74-75 1.4091191618827352 1108 76 1.1666328129592216 1109 1 0.39240260851461883 1109 2 -0.16263941035745333 1109 3 0.4759011189802429 1109 4 -0.0019197444188101542 1109 5 0.2616997572877757 1109 6 1.2157912401768414 1109 7 -1.3881641966002505 1109 8 0.8463188950062346 1109 9 1.202213261735892 1109 10-11 1.430065056065942 1109 12-13 0.516340166144559 1109 14-15 0.7725080406377884 1109 16-17 -0.35655958959124234 1109 18-19 0.32623182050904376 1109 20-21 1.4176836361225256 1109 22-23 1.1261223914461098 1109 24-25 1.3810751860759254 1109 26-27 0.7398395674891773 1109 28-29 0.44822548116423633 1109 30-31 0.6563838388880399 1109 32-33 1.253678998579879 1109 34-35 0.539959848861649 1109 36-37 0.9058708526988823 1109 38-39 1.1239073128076456 1109 40-41 0.956625881616084 1109 42-43 1.0161591113101665 1109 44-45 -0.07441003515673827 1109 46-47 0.6520916576168432 1109 48-49 0.4119913885974711 1109 50-51 1.0984022203195138 1109 52-53 1.098761423671526 1109 54-55 0.6097096186782025 1109 56-57 1.144226425214697 1109 58-59 0.8812057522166938 1109 60-61 1.1522699945268542 1109 62-63 0.6300215232739887 1109 64-65 0.9702417840872286 1109 66-67 1.050084868488149 1109 68-69 1.4058626947645863 1109 70-71 0.8396492015506425 1109 72-73 0.9513235395565331 1109 74-75 2.252040654804233 1109 76 0.5277653740917856 1110 1 1.3066883228003334 1110 2 0.5802177324996904 1110 3 0.41399635707547944 1110 4 0.5552231127240468 1110 5 0.5283664239544414 1110 6 0.7872198116054179 1110 7 1.1880262795902254 1110 8 2.8939379426252785 1110 9 -0.36921530969267735 1110 10-11 -0.46279208679119677 1110 12-13 0.804435404239797 1110 14-15 0.5606032787330264 1110 16-17 0.5434404104087562 1110 18-19 0.9976603919376146 1110 20-21 0.27720744564633293 1110 22-23 0.9594557247794384 1110 24-25 0.9501228051235486 1110 26-27 -0.6411128134632023 1110 28-29 0.6791778621166245 1110 30-31 0.6040028865070823 1110 32-33 1.8155837604846354 1110 34-35 1.0899598488616533 1110 36-37 0.38206132888935684 1110 38-39 0.8143835032838354 1110 40-41 1.1423401673303673 1110 42-43 1.4518733970244497 1110 44-45 1.8851137743670776 1110 46-47 1.0020916576168446 1110 48-49 1.9334199600260433 1110 50-51 1.05078317270047 1110 52-53 1.232094757004866 1110 54-55 -0.18314752417894198 1110 56-57 0.8156549966432678 1110 58-59 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44-45 2.442640656087505 2116 46-47 0.6411853596137718 2116 48-49 0.9868761812242042 2116 50-51 0.22374784243933732 2116 52-53 1.369575555775981 2116 54-55 -0.4615653429193358 2116 56-57 -0.449552376628624 2116 58-59 -0.585921743943068 2116 60-61 -1.663244598407097 2116 62-63 0.32049771375017855 2116 64-65 0.5601035352393069 2116 66-67 -0.3287937797453395 2116 68-69 0.2362006363928444 2116 70-71 -2.275327756974704 2116 72-73 0.29371985292059577 2116 74-75 -1.7492650287595204 2116 76 -1.5349074369681226 2117 1 -0.393158888811584 2117 2 0.3206811906102054 2117 3 0.6613862221124016 2117 4 0.09741817256616869 2117 5 -0.08788007464499614 2117 6 -1.066867277775792 2117 7 -2.4786658518077864 2117 8 -2.7061893810314537 2117 9 -0.21678342784903037 2117 10-11 -0.14937217337129027 2117 12-13 0.6092355060987273 2117 14-15 -0.5797201233550808 2117 16-17 0.13600470885540972 2117 18-19 0.1544085457445945 2117 20-21 -0.2784075276157054 2117 22-23 0.35785144670457214 2117 24-25 0.3103596271250737 2117 26-27 0.7248026436287205 2117 28-29 0.4823609535350002 2117 30-31 0.26870876886003003 2117 32-33 1.336974134816181 2117 34-35 -0.5293806145964552 2117 36-37 -1.4894436367332489 2117 38-39 0.6574443639917575 2117 40-41 -1.154540403079615 2117 42-43 -0.8957456505945984 2117 44-45 0.39304603818169426 2117 46-47 -0.0898105578147792 2117 48-49 -0.8173185171830255 2117 50-51 0.6862428111012875 2117 52-53 0.8254866592202958 2117 54-55 -1.2986937426663374 2117 56-57 -1.1455876822464717 2117 58-59 -0.22783422741152037 2117 60-61 1.147418963205233 2117 62-63 -0.8746894520252226 2117 64-65 -0.36054507611139286 2117 66-67 -0.9606867128716701 2117 68-69 -0.48792391078672637 2117 70-71 0.9159924661292038 2117 72-73 1.109917886386178 2117 74-75 -0.5356344152237824 2117 76 -0.8733296093561407 2118 1 -0.4734005002936712 2118 2 0.6209776634150614 2118 3 0.4397797274184363 2118 4 0.73874149416989 2118 5 0.1266886544010255 2118 6 1.2039481609762532 2118 7 1.36026906271384 2118 8 -3.1332764664544115 2118 9 -0.8221143967886562 2118 10-11 0.03503668202202448 2118 12-13 1.16689531048209 2118 14-15 -0.5174746882050414 2118 16-17 0.28893757300436107 2118 18-19 -1.300649501968138 2118 20-21 -2.8062122434728423 2118 22-23 -0.6260365031998347 2118 24-25 -0.09950709865143637 2118 26-27 0.8283049018094317 2118 28-29 -0.23424429431072014 2118 30-31 0.001412212931960255 2118 32-33 -0.05229188718376321 2118 34-35 0.9473864464436943 2118 36-37 0.3460139565725555 2118 38-39 -0.12657381228487452 2118 40-41 -0.9758809034813822 2118 42-43 -0.21439850033552688 2118 44-45 1.2358046206537736 2118 46-47 0.20660683156206971 2118 48-49 -1.0426101411335935 2118 50-51 -0.17311270689489078 2118 52-53 -1.2377424006561313 2118 54-55 0.27521665050647925 2118 56-57 1.350394695631671 2118 58-59 0.6717806350195517 2118 60-61 -1.3385886287151934 2118 62-63 0.3567671438019957 2118 64-65 -1.2125857510719058 2118 66-67 -2.5394043987213273 2118 68-69 1.0554802619987314 2118 70-71 -0.868912357788119 2118 72-73 -1.41662612735686 2118 74-75 0.33696540190513957 2118 76 -0.208454709796694 2119 1 -0.04792888319808597 2119 2 0.5198125759619359 2119 3 0.6214417661783465 2119 4 0.3417529732870612 2119 5 0.5617786838860432 2119 6 1.1084510665383291 2119 7 -2.052778771712063 2119 8 0.5668924282869483 2119 9 -0.5878946046150659 2119 10-11 0.07940733441374448 2119 12-13 0.603001571038007 2119 14-15 -0.5521696757526371 2119 16-17 -0.2066253617564655 2119 18-19 -0.6835682321086907 2119 20-21 -2.025002499105053 2119 22-23 0.6307264430114827 2119 24-25 0.5457423789199183 2119 26-27 0.6061510644636598 2119 28-29 0.7381886487517164 2119 30-31 -0.045720870398987756 2119 32-33 -0.09601844946011795 2119 34-35 1.023016939101062 2119 36-37 -0.47145090473336637 2119 38-39 0.12327590002153954 2119 40-41 -1.165486720330776 2119 42-43 -0.9592815621968072 2119 44-45 -1.0221606271062171 2119 46-47 -0.9279793763215949 2119 48-49 -0.4997423656777187 2119 50-51 0.5169368164784274 2119 52-53 -1.7452138459943498 2119 54-55 -1.5377252668782297 2119 56-57 0.14598911924257862 2119 58-59 0.7383223004934578 2119 60-61 0.6103994341480004 2119 62-63 -0.784474661940429 2119 64-65 0.06455906901225461 2119 66-67 -0.2110043185678947 2119 68-69 -0.9292333325966382 2119 70-71 -0.923860401185479 2119 72-73 -1.7789053475784264 2119 74-75 0.00352710573819337 2119 76 -0.37233986137256636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 10.0 13 42.0 14 123.0 15 319.0 16 483.0 17 565.0 18 613.0 19 597.0 20 573.0 21 554.0 22 548.0 23 535.0 24 644.0 25 812.0 26 1001.0 27 1164.0 28 1485.0 29 1794.0 30 2183.0 31 2791.0 32 3638.0 33 4702.0 34 6470.0 35 9436.0 36 15666.0 37 18352.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.55027363451014 21.7767464320206 10.830024680759738 28.84295525270952 2 18.48069241011984 19.424766977363515 34.67909454061252 27.415446071904125 3 17.66977363515313 23.41278295605859 29.720372836218374 29.197070572569906 4 13.311584553928096 18.447403462050598 36.50332889480693 31.73768308921438 5 13.575233022636485 35.275632490013315 35.840213049267646 15.308921438082557 6 28.635153129161118 36.29027962716378 16.80426098535286 18.270306258322236 7 27.868175765645802 30.339547270306262 21.544607190412783 20.247669773635153 8 24.32490013315579 32.51131824234354 18.793608521970707 24.37017310252996 9 23.909454061251665 16.091877496671106 21.320905459387486 38.67776298268974 10-11 24.952063914780293 25.249001331557924 26.95139813581891 22.847536617842877 12-13 24.149800266311587 23.649800266311587 26.21970705725699 25.980692410119836 14-15 21.894141145139816 24.88548601864181 23.758988015978694 29.46138482023968 16-17 22.191744340878827 27.286950732356857 26.127163781624503 24.394141145139812 18-19 21.75699067909454 26.719041278295606 26.927430093209054 24.596537949400798 20-21 21.43209054593875 27.322237017310254 26.54593874833555 24.699733688415446 22-23 22.72969374167776 27.14114513981358 26.637816245006658 23.491344873501998 24-25 22.29693741677763 27.342210386151798 26.44141145139813 23.91944074567244 26-27 21.1491344873502 27.9474034620506 26.833555259653796 24.069906790945407 28-29 22.27896138482024 26.66311584553928 27.88215712383489 23.175765645805594 30-31 22.29294274300932 26.96537949400799 27.02596537949401 23.715712383488682 32-33 20.818242343541943 28.252330226364847 27.0219707057257 23.90745672436751 34-35 22.09786950732357 27.228362183754996 27.284953395472705 23.388814913448737 36-37 22.288948069241012 27.268308921438084 26.894141145139816 23.548601864181094 38-39 21.752330226364847 27.290279627163784 27.730359520639148 23.227030625832224 40-41 22.08255659121172 26.26897470039947 27.877496671105195 23.77097203728362 42-43 21.279627163781626 25.673768308921442 28.191078561917443 24.855525965379492 44-45 21.03728362183755 25.962716378162447 28.308255659121173 24.69174434087883 46-47 21.03129161118509 25.532623169107854 28.95406125166445 24.48202396804261 48-49 20.31491344873502 25.555925432756325 29.613848202396802 24.51531291611185 50-51 20.049933422103862 25.93475366178429 29.171105193075896 24.844207723035954 52-53 19.3508655126498 26.063249001331556 29.386817576564578 25.199067909454058 54-55 18.747669773635153 26.524633821571236 29.025965379494007 25.7017310252996 56-57 18.80093209054594 26.50399467376831 29.0612516644474 25.633821571238347 58-59 19.139813581890813 26.000665778961384 28.774300932090547 26.085219707057256 60-61 19.298934753661783 25.902130492676434 28.783621837549934 26.01531291611185 62-63 19.36085219707057 26.204394141145137 28.687749667110516 25.74700399467377 64-65 20.19041278295606 25.85619174434088 27.648468708388812 26.304926764314246 66-67 20.249001331557924 26.012649800266313 26.94340878828229 26.794940079893475 68-69 20.08122503328895 25.488681757656455 26.80958721704394 27.620505992010653 70-71 20.458055925432756 26.13848202396804 26.42276964047936 26.98069241011984 72-73 20.977363515312916 26.133821571238347 25.701065246338217 27.187749667110516 74-75 20.724367509986685 26.931424766977365 25.363515312916114 26.98069241011984 76 21.44607190412783 26.866844207723034 24.96005326231691 26.72703062583222 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.5 16 5.0 17 7.0 18 7.5 19 10.5 20 16.5 21 20.0 22 21.5 23 40.0 24 68.0 25 79.0 26 120.5 27 212.0 28 315.0 29 368.0 30 425.5 31 563.5 32 840.0 33 1036.0 34 1198.0 35 1600.0 36 1987.5 37 2135.0 38 2469.5 39 3122.5 40 3794.0 41 4289.0 42 4431.0 43 4650.0 44 5017.5 45 5271.0 46 5376.0 47 5404.0 48 5292.5 49 4933.0 50 4713.0 51 4456.5 52 3861.0 53 3166.5 54 2811.0 55 2669.5 56 2217.5 57 1720.5 58 1534.0 59 1303.0 60 954.5 61 726.5 62 616.0 63 558.5 64 454.5 65 348.0 66 287.0 67 286.0 68 235.5 69 153.0 70 98.5 71 76.0 72 60.0 73 43.5 74 30.5 75 18.0 76 20.0 77 16.5 78 7.5 79 4.0 80 5.0 81 5.5 82 4.0 83 3.0 84 3.5 85 2.5 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 1.5 98 2.0 99 3.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7296937416777629 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.094540612516646 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42709002681084 45.32756324900133 2 11.619507655491791 13.96537949400799 3 5.020939044116018 9.051930758988016 4 2.6988101305089627 6.487350199733688 5 1.4646252021891826 4.400798934753661 6 1.0569231791894707 3.810918774966711 7 0.6492211561897587 2.7310252996005326 8 0.5472956504398307 2.63115845539281 9 0.38554430435842324 2.085219707057257 >10 1.1300436507057234 9.508655126498002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.007989347536617843 0.0 5 0.0 0.0 0.0 0.009320905459387484 0.0 6 0.0 0.0 0.0 0.009320905459387484 0.0 7 0.0 0.0 0.0 0.009320905459387484 0.0 8 0.0 0.0 0.0 0.013315579227696404 0.0 9 0.0 0.0 0.0 0.017310252996005325 0.0 10 0.0 0.0 0.0 0.022636484687083888 0.0 11 0.0 0.0 0.0 0.02929427430093209 0.0 12 0.0 0.0 0.0 0.03861517976031957 0.0 13 0.0 0.0 0.0 0.03994673768308921 0.0 14 0.0 0.0 0.0 0.043941411451398134 0.0 15 0.0 0.0 0.0 0.04660452729693742 0.0 16 0.0 0.0 0.0 0.04660452729693742 0.0 17 0.0 0.0 0.0 0.0492676431424767 0.0 18 0.0 0.0 0.0 0.05326231691078562 0.0 19 0.0 0.0 0.0 0.06258322237017311 0.0 20 0.0 0.0 0.0 0.06524633821571238 0.0 21 0.0 0.0 0.0 0.0692410119840213 0.0 22 0.0 0.0 0.0 0.0758988015978695 0.0 23 0.0 0.0 0.0 0.08122503328894808 0.0 24 0.0 0.0 0.0 0.08255659121171771 0.0 25 0.0 0.0 0.0 0.085219707057257 0.0 26 0.0 0.0 0.0 0.09587217043941411 0.0 27 0.0 0.0 0.0 0.12916111850865514 0.0 28 0.0 0.0 0.0 0.1611185086551265 0.0 29 0.0 0.0 0.0 0.1970705725699068 0.0 30 0.0 0.0 0.0 0.22237017310252996 0.0 31 0.0 0.0 0.0 0.27030625832223704 0.0 32 0.0 0.0 0.0 0.30492676431424764 0.0 33 0.0 0.0 0.0 0.34354194407456723 0.0 34 0.0 0.0 0.0 0.3968042609853529 0.0 35 0.0 0.0 0.0 0.4647137150466045 0.0 36 0.0 0.0 0.0 0.6245006657789614 0.0 37 0.0 0.0 0.0 0.9733688415446072 0.0 38 0.0 0.0 0.0 1.4327563249001332 0.0 39 0.0 0.0 0.0 2.0026631158455395 0.0 40 0.0 0.0 0.0 2.66577896138482 0.0 41 0.0 0.0 0.0 3.588548601864181 0.0 42 0.0 0.0 0.0 4.587217043941411 0.0 43 0.0 0.0 0.0 5.579227696404794 0.0 44 0.0 0.0 0.0 6.623169107856191 0.0 45 0.0 0.0 0.0 7.498002663115845 0.0 46 0.0 0.0 0.0 8.499334221038616 0.0 47 0.0 0.0 0.0 9.531291611185086 0.0 48 0.0 0.0 0.0 10.451398135818907 0.0 49 0.0 0.0 0.0 11.249001331557922 0.0 50 0.0 0.0 0.0 12.006657789613849 0.0 51 0.0 0.0 0.0 12.865512649800266 0.0 52 0.0 0.0 0.0 13.917443408788282 0.0 53 0.0 0.0 0.0 14.970705725699068 0.0 54 0.0 0.0 0.0 15.958721704394142 0.0 55 0.0 0.0 0.0 16.821571238348866 0.0 56 0.0 0.0 0.0 17.73102529960053 0.0 57 0.0 0.0 0.0 18.58988015978695 0.0 58 0.0 0.0 0.0 19.203728362183757 0.0 59 0.0 0.0 0.0 19.942743009320907 0.0 60 0.0 0.0 0.0 20.584553928095872 0.0 61 0.0 0.0 0.0 21.275632490013315 0.0 62 0.0 0.0 0.0 22.0 0.0 63 0.0 0.0 0.0 22.796271637816243 0.0 64 0.0 0.0 0.0 23.679094540612518 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCAAG 15 0.0022178816 69.99134 33 CAAGGGT 15 0.0022178816 69.99134 36 CCAAGGG 15 0.0022178816 69.99134 35 GACTAGA 15 0.0022178816 69.99134 16 GTTGACT 20 0.006930277 52.493507 54 TCGTTCG 20 0.006930277 52.493507 23 CGTTCGG 20 0.006930277 52.493507 24 GTAATCA 20 0.006930277 52.493507 56 ACAGGAC 20 0.006930277 52.493507 19 TTCGGTA 20 0.006930277 52.493507 26 AAGGGTC 20 0.006930277 52.493507 37 TACAGGA 20 0.006930277 52.493507 18 TTACCTA 20 0.006930277 52.493507 30 TATACCG 20 0.006930277 52.493507 6 CTGGTGT 30 5.8477424E-4 46.660892 11 ATATAGC 35 0.0012490497 39.995052 6 CATTTTT 35 0.0012490497 39.995052 14 GTGTAAG 40 0.0023059736 35.30121 1 GTGTGGA 40 0.0024065713 34.99567 11 GACTGGT 40 0.0024065713 34.99567 9 >>END_MODULE