FastQCFastQC Report
Wed 25 May 2016
SRR1780672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75100
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT5800.7723035952063915No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3180.42343541944074564RNA PCR Primer, Index 24 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC940.12516644474034622No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC920.12250332889480692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGT150.00221679470.010
TGAATCT150.00221679470.057
TACCAAG200.0069268952.521
ACGTTAG200.0069268952.570
GACGTTA200.0069268952.569
TGGAATA200.0069268952.538
CTGAGGA200.0069268952.562
TAGCGGA200.0069268952.534
TTTCCAT200.0069268952.529
ACCTAGT200.0069268952.546
TAACACC200.0069268952.536
ATCCCAT200.0069268952.527
CATACCA200.0069268952.519
CTCGAGT200.0069268952.562
CGGTCTG200.0069268952.510
TTGTTCG200.0069268952.59
CATACAT305.844179E-446.66666431
GCGGCTT305.844179E-446.66666411
CATGGTC350.001248290240.0000049
ACAAAGT501.6953364E-435.041
Produced by FastQC (version 0.11.5)