FastQCFastQC Report
Wed 25 May 2016
SRR1780671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4029.629629629629626No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT1410.37037037037037No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC85.9259259259259265No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG75.185185185185185No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC21.4814814814814816No Hit
CTTATACACATCTCCGAGCCCACGTGACCCAAATAAATCTCGGATGCCGT10.7407407407407408No Hit
CTCCACCGTGGTCCGATTCCCGGGCCGCACCGCGCGCCGGCGCCGCGCCA10.7407407407407408No Hit
CACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTCTCCTCCTA10.7407407407407408No Hit
GGTAAAATGGCTGAGTGAAGCATTGGACTGTAAATCTAAAGACAGGGGTT10.7407407407407408No Hit
TCACCGCCCTGCCCGATGGAGCGCACACGCTGCGATAGGCCTGTCTCTTA10.7407407407407408No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC10.7407407407407408No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCTGTCTCTT10.7407407407407408No Hit
GTACGCACCGTCTGCAAAGTCTCACCGGATGGGGACACTCTCCTAGTTGT10.7407407407407408No Hit
GGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGGATTAGAA10.7407407407407408No Hit
TCTCTCTCCGCTCCTTACTCCGCCCACCGCAGTTTCCTCCAATCGCGGCC10.7407407407407408No Hit
GTCCCCGGCCCTGCTCGCGCTGGGCCTCAGCGAGGACGAGGCCCGCACCT10.7407407407407408No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTAGTATGCCGT10.7407407407407408No Hit
CTTCAGACTACAGATGTAATTAAAGGCTTTTCTTAATATGTTTTAACCAA10.7407407407407408No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC10.7407407407407408No Hit
TCTTATACACATCTCCGAGCCCACGAGAGACGCTCCATCTCGTATGCCGT10.7407407407407408No Hit
ATACACATCTCCGAGCCCAGAGACCGGTTCCCATCTCGTATGCCGTCTTC10.7407407407407408RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTGCTCTGTCGGGTTAACTATTGAACTAGTCAGAGGGCTCACTTATC10.7407407407407408No Hit
TGACAGGATGGACTTTGTTCTCAGGGGCCAGGTGTTTCATTAGGGCTGTC10.7407407407407408No Hit
AACTTACTATCCGCCATCCCATACATTGGGACAGACCTAGTTCAATGAAT10.7407407407407408No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGC10.7407407407407408No Hit
GGACGCGGACGGCGTCGTCGATCTGGTCCCGCGGGACGTCCGCCGTCGCT10.7407407407407408No Hit
CTTATACACATCTCCGAGCCCAGAGACTTAATGCATCTCGTATGCCGTCT10.7407407407407408No Hit
CGCCTATCTCGCGGGCACGTGGTCGTCGCCGCGCGCGGGTCGGCTGCTGT10.7407407407407408No Hit
TCAACATACTGCTCGATGATCGGCCGCAGTCCCGACGGAAGCGGCCTGTA10.7407407407407408No Hit
TTTCAGTCTCAGCTAATGGTGTCGCATTTCACCCAGTCTGTCTCTTATAC10.7407407407407408No Hit
GCATTCCACGCCACCGGGCGGCACACCTGTCTCTTATACACATCTCCGAG10.7407407407407408No Hit
GTCCGGGGTCGGTCCTACCATGGGCGCATGCCCCAGACCTGTCTCTTATA10.7407407407407408No Hit
CACGTGGCGGGCATCGGCCACGGGCAGCGCTACGGCTGTCTCTTATACAC10.7407407407407408No Hit
GCCGATCGGCACGTCGAGGATCTCGGCGATCTCCTTGTATGCGAAGCCCT10.7407407407407408No Hit
TTCCCGTACTAATTAATCCCCTGGCCCAACCCGTCATCTACCTGTCTCTT10.7407407407407408No Hit
CTCTCCAGGAGGACGGCGGGGACGGGACCGAGCGTGGGGGCTGGGCCGCT10.7407407407407408No Hit
CCTGGATCCAGCCCGGCATGTCCGTGGCGTCCCTGGACCTGTCTCTTATA10.7407407407407408No Hit
CAACTATCACACATCAACTGCAACTCCAAAGCCACCCCTCACCCACTAGG10.7407407407407408No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT10.7407407407407408No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTGGTATGCCG10.7407407407407408No Hit
CCGCCGAAGGCCCCCATCGGACCGGCGGCGGTGCCGGCCGTCCACTGGAC10.7407407407407408No Hit
CTTATACACATCTCCGAGCCCACGAACCGGTTCCCATCTCGTATGCCGTC10.7407407407407408No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC10.7407407407407408No Hit
GGCCCCAGCGGGTCACGTTGACCACGGCCTCGCCGATGATGCCGCCGCTC10.7407407407407408No Hit
GTGTGGACGTTCCGCCCCGAGGCCAACGCGGCCCGCCTGAACCTGTCTCT10.7407407407407408No Hit
GAAGCAGCCCGAGGTGACCCTGCTGCTGCGCCTGCGCGGGCTGTCTCTTA10.7407407407407408No Hit
ATCTCCGAGCCCACGAGACCCAAATAAATTTCGTATGCCGTCTTCTGCTT10.7407407407407408RNA PCR Primer, Index 19 (96% over 25bp)
GCCAACGCCACTTATCCAGTGAACCACTATCACGAAAAAAACTCTACCTC10.7407407407407408No Hit
CATCGTCACGGCGTGGGCCTGCAGGTGGTCCGGGTGGGGGTAGCCGCCGA10.7407407407407408No Hit
ATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTT10.7407407407407408RNA PCR Primer, Index 39 (96% over 27bp)
ATCTCCGAGACCACGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTT10.7407407407407408TruSeq Adapter, Index 1 (96% over 30bp)
CTGCTGGCCCTCACCCTCGACACCCCCGCGACCAGCGGCGCCGGCCCCCA10.7407407407407408No Hit
CTGCAGGTCTACCTGCGCCAGCACCACGGACACCGCGACGACGCCCGGCT10.7407407407407408No Hit
GTCGTGGACGGCGTGGACGCCGGCGAGCAGGTGCGCGTGCTGAACGGCAC10.7407407407407408No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT10.7407407407407408No Hit
GGCCCGGACCGTGTTCGCGACGCGGCGGGCCACGCGCTGGATGACGGCGG10.7407407407407408No Hit
ATACACATCGCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT10.7407407407407408No Hit
CTGATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT10.7407407407407408No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA10.7407407407407408No Hit
TCTCCGAGCCCACGAGCCCACGAGACTTCGCGGCATCTCCTTTTTCCCCT10.7407407407407408No Hit
CTCATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCC10.7407407407407408No Hit
CCAGAACACTACGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT10.7407407407407408RNA PCR Primer, Index 34 (95% over 23bp)
GGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATATGGTCTGT10.7407407407407408No Hit
TCCCGGTGCTGATGTTCCTCTACCCGATCGCCGTCGTCGTCATCCTGCTC10.7407407407407408No Hit
GTGGACGATGGACCCGGCCGACGGCGCCCCCGGCTCCGGTCTCGAGGCGC10.7407407407407408No Hit
GAGGTGGAGTGCTGCTAGGGCTGCAATAATGAAGGGCAAGATGAAGTGAA10.7407407407407408No Hit
CTTATACACATCTCCGACCACGAGACGATCCAAAATCTCGTATGCCGTCT10.7407407407407408TruSeq Adapter, Index 6 (95% over 21bp)
CCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACCCCTTAAA10.7407407407407408No Hit
CTTATACACATCTCCGAGCCTACGAGACCCAAATAAATCTCGTATGCCGT10.7407407407407408No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers