FastQCFastQC Report
Wed 25 May 2016
SRR1780670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146326
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCATT308.348818E-658.32735433
ATTCCCG252.3853302E-455.99426331
TTCCCGG252.3853302E-455.99426332
TCCCGGA252.3853302E-455.99426333
CCCGGAC252.3853302E-455.99426334
AGCGTGG200.006942792352.49461758
GAGTATC200.006942792352.4946177
CAACCGT200.006942792352.4946176
GGATCCG200.006942792352.49461760
TGTATAA200.006942792352.4946172
CCAGAGG200.006942792352.49461747
GACGGTT200.006942792352.49461711
CACAGTG200.006942792352.4946177
GGACGTC200.006942792352.49461737
CAGACGG200.006942792352.4946179
CGGACGT200.006942792352.49461736
GCAATTC305.8654655E-446.66188428
TGCAATT305.8654655E-446.66188427
GAGGCGC305.8654655E-446.66188429
GATGCAA350.001252812139.995925
Produced by FastQC (version 0.11.5)