##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780670_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 146326 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.912155051050394 34.0 31.0 34.0 12.0 34.0 2 31.2400940366032 34.0 32.0 34.0 23.0 34.0 3 31.45490890204065 34.0 33.0 34.0 23.0 34.0 4 31.894509519839264 34.0 34.0 34.0 23.0 34.0 5 31.87258586990692 34.0 34.0 34.0 23.0 34.0 6 34.792347224690076 38.0 36.0 38.0 26.0 38.0 7 34.059223924661374 38.0 34.0 38.0 23.0 38.0 8 33.23264491614614 37.0 34.0 38.0 21.0 38.0 9 33.75921572379482 37.0 34.0 38.0 21.0 38.0 10-11 34.14259598430901 37.5 34.0 38.0 23.5 38.0 12-13 34.48526577641704 38.0 35.5 38.0 25.0 38.0 14-15 34.075581236417314 38.0 34.0 38.0 23.0 38.0 16-17 33.90673906209423 37.5 34.0 38.0 22.0 38.0 18-19 33.8998913385181 37.5 34.0 38.0 22.0 38.0 20-21 33.98354701146754 38.0 34.0 38.0 22.5 38.0 22-23 33.96454833727431 37.5 34.0 38.0 22.5 38.0 24-25 34.34784317209518 38.0 35.0 38.0 23.5 38.0 26-27 34.41400024602599 38.0 35.5 38.0 24.0 38.0 28-29 34.536838975985134 38.0 36.0 38.0 24.5 38.0 30-31 34.40539617019532 38.0 35.0 38.0 24.0 38.0 32-33 34.142476388338366 38.0 34.0 38.0 23.0 38.0 34-35 34.041059005234885 37.0 34.0 38.0 23.0 38.0 36-37 34.00026652816314 37.5 34.0 38.0 22.5 38.0 38-39 34.53504845345325 38.0 35.5 38.0 25.5 38.0 40-41 34.02637603706792 37.5 34.0 38.0 23.0 38.0 42-43 34.34921340021596 38.0 35.0 38.0 24.0 38.0 44-45 33.42937003676722 37.0 34.0 38.0 21.5 38.0 46-47 34.147458414772494 37.0 34.0 38.0 24.0 38.0 48-49 33.87611907658243 37.0 34.0 38.0 23.0 38.0 50-51 33.813778822628926 37.0 34.0 38.0 22.5 38.0 52-53 33.8245732132362 37.0 34.0 38.0 23.0 38.0 54-55 33.50837171794486 37.0 34.0 38.0 21.5 38.0 56-57 33.64131118188155 37.0 34.0 38.0 22.0 38.0 58-59 33.50992304853546 37.0 33.5 38.0 22.0 38.0 60-61 33.52591473832402 37.0 33.5 38.0 21.5 38.0 62-63 33.62690157593319 37.0 34.0 38.0 22.5 38.0 64-65 33.62596189330672 37.0 34.0 38.0 22.5 38.0 66-67 33.55045924852726 37.0 33.5 38.0 22.0 38.0 68-69 33.13245424599866 37.0 32.5 38.0 20.5 38.0 70-71 33.01612837089786 37.0 32.0 38.0 20.5 38.0 72-73 33.1545453302899 37.0 32.0 38.0 20.5 38.0 74-75 32.792839276683566 37.0 31.5 38.0 19.5 38.0 76 32.040942826291975 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.266803285121192 1101 2 -0.4308720947390725 1101 3 0.43649993756358185 1101 4 -2.1253309527343376 1101 5 -0.9792854965762992 1101 6 -1.927768346308575 1101 7 -1.5219221505957705 1101 8 -0.11751517220438501 1101 9 -1.2964870908240655 1101 10-11 -1.365843168121593 1101 12-13 -0.7526663163962368 1101 14-15 -0.016846654164233144 1101 16-17 -1.7290398745046787 1101 18-19 0.10486722175571828 1101 20-21 -0.7692536130757688 1101 22-23 -0.5631639501124468 1101 24-25 -1.2896129118712523 1101 26-27 -1.3681461587596218 1101 28-29 -0.32074709877363006 1101 30-31 -0.9238536965112871 1101 32-33 -0.002168708455627666 1101 34-35 -1.227956809633767 1101 36-37 -0.23124948979533144 1101 38-39 -1.0881768094009203 1101 40-41 0.3614568041918176 1101 42-43 -0.40623858782108613 1101 44-45 -0.20100428208223065 1101 46-47 0.3556466655092976 1101 48-49 -0.9159070684023547 1101 50-51 -0.7068913027867794 1101 52-53 -0.19527203532149429 1101 54-55 -1.722198041953142 1101 56-57 -0.6958475041345906 1101 58-59 0.20286376738754797 1101 60-61 0.11386549952063518 1101 62-63 -1.5594336757518548 1101 64-65 -0.8602387346354945 1101 66-67 -1.0670823724112992 1101 68-69 0.26431372187852276 1101 70-71 0.217505191588117 1101 72-73 -0.7912959941778794 1101 74-75 -1.3074425288682328 1101 76 -1.7022905930771017 1102 1 0.358951881810448 1102 2 -0.6265000756611698 1102 3 0.2661501760373497 1102 4 0.11767381674101784 1102 5 0.2864537721041458 1102 6 0.30394866482020433 1102 7 0.6500969273056612 1102 8 1.2913878484633372 1102 9 0.6728292843746573 1102 10-11 -0.9890546148624537 1102 12-13 -4.279574106539322 1102 14-15 -1.2364015031308497 1102 16-17 -2.923515232215337 1102 18-19 -1.2415556717259975 1102 20-21 -0.11853819177211022 1102 22-23 -0.98085075456396 1102 24-25 0.025728900529379928 1102 26-27 -5.969020562575203 1102 28-29 -0.8657391496480287 1102 30-31 0.05802229712941198 1102 32-33 -6.779632937390442 1102 34-35 -0.7509297826067396 1102 36-37 0.5166439919216756 1102 38-39 0.5138104719981271 1102 40-41 0.6535474242236106 1102 42-43 -0.020268794498356613 1102 44-45 -5.785026539633893 1102 46-47 -0.44777145849706557 1102 48-49 -0.11431724328311788 1102 50-51 -6.306533592134947 1102 52-53 -1.8552879335726864 1102 54-55 0.8377781106700652 1102 56-57 -1.0857918602554193 1102 58-59 0.6116078055433576 1102 60-61 0.43667948998167816 1102 62-63 -4.019847030282854 1102 64-65 -1.1623452529184846 1102 66-67 0.033275338240521535 1102 68-69 -7.834056707374259 1102 70-71 -0.47080959378548926 1102 72-73 0.173688107570932 1102 74-75 -6.278467966070139 1102 76 -2.1154861415667696 1103 1 0.08742913955599718 1103 2 -0.4224789813961749 1103 3 0.16153580556932212 1103 4 0.722947382990192 1103 5 0.3672711748727693 1103 6 -0.7853723635252123 1103 7 1.037044850838953 1103 8 1.5199310257870025 1103 9 0.44296771548869884 1103 10-11 0.6153619968712292 1103 12-13 -0.8450981671853341 1103 14-15 -0.7287548320695478 1103 16-17 -0.5775692862693944 1103 18-19 0.07093608832673937 1103 20-21 -0.5653081324444926 1103 22-23 -0.062195513957497894 1103 24-25 1.0479767581430934 1103 26-27 -0.5244589277696647 1103 28-29 0.823944436377019 1103 30-31 0.33920225757766076 1103 32-33 -4.79617875149723 1103 34-35 -1.0072251023166956 1103 36-37 -0.16414045105788944 1103 38-39 0.4408045392360975 1103 40-41 0.5964281097872188 1103 42-43 0.6650311395820694 1103 44-45 -4.357603995138181 1103 46-47 -0.033664668780517104 1103 48-49 0.10410727879993686 1103 50-51 -4.051369065649624E-5 1103 52-53 -0.1712404714764446 1103 54-55 0.5455566208876022 1103 56-57 0.9261395833833461 1103 58-59 0.6233085306587185 1103 60-61 1.1017421135808902 1103 62-63 0.7348991793414044 1103 64-65 0.8434556699556097 1103 66-67 0.21903670936774944 1103 68-69 -2.5952630356537583 1103 70-71 0.6811350337688964 1103 72-73 1.2687111794232706 1103 74-75 -1.1488700754966388 1103 76 -0.0024340651000542834 1104 1 -2.4175772789292402 1104 2 -0.5399489312152888 1104 3 0.2004318261084741 1104 4 0.10043600476636527 1104 5 -0.5284692849241743 1104 6 -0.46817082308875513 1104 7 0.6318633827563289 1104 8 1.6943621145954992 1104 9 -0.4087302656059464 1104 10-11 -0.4642388900467296 1104 12-13 -0.846889508065253 1104 14-15 -0.35108532570941264 1104 16-17 -0.6763731140205849 1104 18-19 0.3284107061351236 1104 20-21 0.6400483852355237 1104 22-23 -0.044778079017596895 1104 24-25 -0.04904156371476631 1104 26-27 0.30783188796145566 1104 28-29 -0.5852981823649586 1104 30-31 -1.4677180365056586 1104 32-33 -0.9311653310280974 1104 34-35 0.07276089371551109 1104 36-37 -0.3923176012504399 1104 38-39 -0.5589620613007185 1104 40-41 0.4195696665616424 1104 42-43 -0.0016860969682852556 1104 44-45 -3.317342587739418 1104 46-47 -0.20078451940486275 1104 48-49 0.5726800410825845 1104 50-51 0.677185533689439 1104 52-53 -0.5999019255551019 1104 54-55 -0.6975639299351357 1104 56-57 0.21222314039115986 1104 58-59 0.3014565059183738 1104 60-61 -0.2176008669302547 1104 62-63 0.3180360681265668 1104 64-65 -0.32154608054036515 1104 66-67 -0.2181730000499229 1104 68-69 -3.802031620086016 1104 70-71 -1.031439362309996 1104 72-73 0.33960043137799545 1104 74-75 -1.181907769509941 1104 76 -0.3517980515628878 1105 1 -0.8781348486254537 1105 2 -0.2699038119390167 1105 3 -1.0510996153217036 1105 4 -0.05891345937119752 1105 5 0.09808630725211742 1105 6 -0.10024983296499101 1105 7 0.8686884404339921 1105 8 0.7162000716128532 1105 9 1.02310212253348 1105 10-11 1.1210018786857319 1105 12-13 -0.9263738754293556 1105 14-15 0.19257069947556005 1105 16-17 0.9964212125429626 1105 18-19 0.40195398995468423 1105 20-21 -0.1350144067043786 1105 22-23 0.23726569918193974 1105 24-25 0.2553430752741974 1105 26-27 0.16968393764948786 1105 28-29 -0.45921992258248423 1105 30-31 0.18919646417056413 1105 32-33 0.8030883825042707 1105 34-35 0.5396203875158747 1105 36-37 1.0366735226400436 1105 38-39 0.5064438417098813 1105 40-41 0.517391553388407 1105 42-43 0.5781647934839285 1105 44-45 1.284653116269041 1105 46-47 0.8692696919170118 1105 48-49 0.2948725878782099 1105 50-51 0.05737794410438113 1105 52-53 -0.7594190242833463 1105 54-55 -0.44579382230804043 1105 56-57 0.07635104274259419 1105 58-59 0.26244878904467583 1105 60-61 -0.06537161100053623 1105 62-63 0.16728142834204363 1105 64-65 0.3150476982274384 1105 66-67 0.4705835490641732 1105 68-69 1.28575776267774 1105 70-71 1.1505777080056134 1105 72-73 0.9778705560526717 1105 74-75 -0.503058708831901 1105 76 -0.7540301487568577 1106 1 -0.27654640717621604 1106 2 -0.1862528516930162 1106 3 -0.7774003512341068 1106 4 -0.3701325943212055 1106 5 -0.2426613895691574 1106 6 -0.7564885165472077 1106 7 -0.12335093916546214 1106 8 0.7977151059533867 1106 9 0.7421358001928056 1106 10-11 0.21622857530445572 1106 12-13 0.04273192041787155 1106 14-15 -0.25698953302120486 1106 16-17 0.28431701864016645 1106 18-19 -0.6055370950097441 1106 20-21 -0.5702492051110326 1106 22-23 -0.7307844091487752 1106 24-25 0.2489567795712091 1106 26-27 -0.21458938721379894 1106 28-29 -1.1662175350101407 1106 30-31 -0.7677965449684976 1106 32-33 0.6065218220201274 1106 34-35 0.4905186425626127 1106 36-37 0.2874729604250632 1106 38-39 0.11708234852581967 1106 40-41 -0.05689324900776427 1106 42-43 0.32679454791382767 1106 44-45 0.6394653941603607 1106 46-47 0.37434251594248735 1106 48-49 0.3079643406263699 1106 50-51 0.2285282138570892 1106 52-53 -0.7219022222800007 1106 54-55 0.02435538561943673 1106 56-57 0.4587922587473017 1106 58-59 -0.3069933537554803 1106 60-61 0.8067367565505705 1106 62-63 0.2577421868412486 1106 64-65 0.5434966354815387 1106 66-67 0.8701459297622804 1106 68-69 1.3472024587686278 1106 70-71 -0.011632975306540061 1106 72-73 0.24092394806258 1106 74-75 -1.4529319649527395 1106 76 -0.395268249255853 1107 1 0.2186162855506808 1107 2 0.4906640078536242 1107 3 0.13520358964052548 1107 4 0.36793640160075824 1107 5 0.5079601125678295 1107 6 0.7416071764123657 1107 7 1.1956970297778042 1107 8 1.4935525724290102 1107 9 -0.5452186213509691 1107 10-11 -0.0811066194737009 1107 12-13 0.7985673050144086 1107 14-15 0.7263370648209886 1107 16-17 0.9468382966690001 1107 18-19 1.421496717155776 1107 20-21 1.3061575940611192 1107 22-23 -0.3366724530396894 1107 24-25 0.23617465434006846 1107 26-27 0.6671200804374777 1107 28-29 -0.7106411606637835 1107 30-31 0.07394390594201639 1107 32-33 1.1264833684888913 1107 34-35 0.4919484036363784 1107 36-37 0.06141150825317254 1107 38-39 0.8451797559740513 1107 40-41 0.7471621289757593 1107 42-43 0.4362830179776296 1107 44-45 0.05529624760832519 1107 46-47 0.8371074962871035 1107 48-49 0.5597829232341098 1107 50-51 1.2603166704499102 1107 52-53 1.344705661918539 1107 54-55 0.7649012053287052 1107 56-57 0.7179035413072867 1107 58-59 0.8862203327625195 1107 60-61 -0.08777932902690111 1107 62-63 -0.40579553803231505 1107 64-65 0.44190093639879535 1107 66-67 1.331879155327755 1107 68-69 1.6366234514575595 1107 70-71 0.805461701206184 1107 72-73 0.6376627457161845 1107 74-75 0.15749335069686765 1107 76 -0.9231715520957806 1108 1 -0.7599995997916373 1108 2 -0.6669103654690502 1108 3 -0.7082555549948886 1108 4 -0.4559829725784219 1108 5 -0.2628786928909719 1108 6 0.05015511223147229 1108 7 0.5819757941242898 1108 8 1.101124090729705 1108 9 -0.31909517020039857 1108 10-11 -0.4073385614596532 1108 12-13 -0.15661741490793446 1108 14-15 0.42390458609089876 1108 16-17 -0.9878102501248094 1108 18-19 -0.2847500709444901 1108 20-21 0.057642205492626886 1108 22-23 -1.1959598395525646 1108 24-25 -1.0855377878457446 1108 26-27 -0.6411794684690548 1108 28-29 0.059089896265355435 1108 30-31 0.34177351823003477 1108 32-33 0.3817965644005028 1108 34-35 0.12445282892044673 1108 36-37 -0.25281221126947173 1108 38-39 0.3746864081752719 1108 40-41 0.0637069813711193 1108 42-43 0.14622420452476348 1108 44-45 0.003283457109837684 1108 46-47 0.4883894011577752 1108 48-49 -0.1226044461486353 1108 50-51 -0.05187712844234227 1108 52-53 -0.009162892869333916 1108 54-55 0.1193150693154621 1108 56-57 0.7078909792105534 1108 58-59 -1.4083694005784295 1108 60-61 0.6592942734398335 1108 62-63 0.08783776294411183 1108 64-65 0.2868732426855516 1108 66-67 0.05901613928252658 1108 68-69 0.9619218012548529 1108 70-71 1.609213200092725 1108 72-73 0.24149989526223692 1108 74-75 0.9571393279708929 1108 76 0.36227354583147076 1109 1 0.06195488481344924 1109 2 -0.33865265377119513 1109 3 0.585401342097029 1109 4 0.4641628589747384 1109 5 0.5376453892532069 1109 6 0.8560795190787189 1109 7 -0.07931333401949558 1109 8 1.34760789830041 1109 9 1.8489586051848335 1109 10-11 1.528615182594578 1109 12-13 0.4704354775128152 1109 14-15 0.3225915324579276 1109 16-17 -0.22780569288877928 1109 18-19 0.7199068546301461 1109 20-21 1.1246762993147215 1109 22-23 1.3261456034962293 1109 24-25 1.3435744056089263 1109 26-27 0.694119172904955 1109 28-29 0.3523651248392241 1109 30-31 0.822120437823294 1109 32-33 1.001540789527965 1109 34-35 0.4338635213354962 1109 36-37 0.8921791014993374 1109 38-39 0.8986261387286874 1109 40-41 0.7725366261915383 1109 42-43 1.3222588395186463 1109 44-45 0.35775666910676307 1109 46-47 -0.006959369812634009 1109 48-49 0.33880715771362446 1109 50-51 1.3500027314226486 1109 52-53 1.4637044630792815 1109 54-55 0.8386662328347896 1109 56-57 1.1602052006352608 1109 58-59 0.20798822851952536 1109 60-61 0.7197061336466177 1109 62-63 -0.004531714967534128 1109 64-65 0.12607401741567514 1109 66-67 0.5590788012780266 1109 68-69 1.5260193261060309 1109 70-71 0.33786397339871854 1109 72-73 0.022467738265454784 1109 74-75 1.8125581664453563 1109 76 -0.2309931379248269 1110 1 0.2672994699048701 1110 2 0.4618680392892216 1110 3 -0.1374762351839962 1110 4 0.3046392632001371 1110 5 0.3255916441534126 1110 6 0.46720559263409456 1110 7 1.3169460549142826 1110 8 2.1842718717271126 1110 9 -0.43609989797224813 1110 10-11 -0.8105564964909178 1110 12-13 0.14310806930740938 1110 14-15 0.43311611575385456 1110 16-17 0.22559527069263652 1110 18-19 0.9236684131183424 1110 20-21 0.02691168848789971 1110 22-23 0.19232748696870772 1110 24-25 0.6017124790446076 1110 26-27 -1.0350486159450298 1110 28-29 0.5296252020454375 1110 30-31 0.23489537273666627 1110 32-33 1.1593167718124278 1110 34-35 0.8220431903662373 1110 36-37 -0.24541553596063181 1110 38-39 0.7340123194151715 1110 40-41 0.6831060968202607 1110 42-43 0.9850681880538232 1110 44-45 1.468206440181369 1110 46-47 0.5689750348317091 1110 48-49 1.0655151207264595 1110 50-51 0.7418242593874638 1110 52-53 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2116 40-41 -0.06742554874936246 2116 42-43 0.2027221964926369 2116 44-45 1.7400545414471864 2116 46-47 0.4154898027641991 2116 48-49 0.5870341080682309 2116 50-51 0.010853795252437237 2116 52-53 1.0465318862471378 2116 54-55 0.026174507066464514 2116 56-57 -0.031161229624785847 2116 58-59 -0.9126264286908778 2116 60-61 -1.4414286181264302 2116 62-63 0.23946828503246564 2116 64-65 0.5031142065409782 2116 66-67 -0.25067060770542327 2116 68-69 0.06841176109421099 2116 70-71 -2.0323771613530575 2116 72-73 0.4301745940574122 2116 74-75 -1.4060895876917634 2116 76 -1.1637023577829844 2117 1 -0.13138434300853774 2117 2 -0.22805574273407814 2117 3 0.1815849742307698 2117 4 -0.1325161610351664 2117 5 -1.2561227566792788 2117 6 -1.0991913452163615 2117 7 -1.730503832605443 2117 8 -2.6468801277356704 2117 9 -0.4209838921971354 2117 10-11 0.06198488617705067 2117 12-13 0.44973265380192373 2117 14-15 -0.710543018651677 2117 16-17 -0.4991873234046693 2117 18-19 -0.4717047863578685 2117 20-21 -0.627538836979646 2117 22-23 -0.284263185562601 2117 24-25 0.49252938801952695 2117 26-27 0.6646592711046324 2117 28-29 0.14393537899201903 2117 30-31 0.01282909331551707 2117 32-33 1.1497777730547512 2117 34-35 -0.40554301653032354 2117 36-37 -1.3819867342952534 2117 38-39 0.05246915720854162 2117 40-41 -0.804571437343526 2117 42-43 -1.022734921102412 2117 44-45 0.254675231102361 2117 46-47 -0.34243415833218194 2117 48-49 -0.4328597911360177 2117 50-51 0.7837273584708342 2117 52-53 1.1056017359376682 2117 54-55 -0.5842162975312348 2117 56-57 -0.42589509346209553 2117 58-59 -0.052378860167443975 2117 60-61 0.696078013173306 2117 62-63 -0.0796864452946835 2117 64-65 -0.0918778358993464 2117 66-67 -1.061344347758471 2117 68-69 -0.18605685075371525 2117 70-71 0.6747528121217456 2117 72-73 1.1170711457815585 2117 74-75 -0.24478454790396142 2117 76 -0.05743180075380394 2118 1 0.5252563063394149 2118 2 0.8724837645759358 2118 3 0.44091554192543114 2118 4 0.7244693851553876 2118 5 0.32070682411176676 2118 6 0.8448702652718296 2118 7 1.21618259521901 2118 8 -3.3820367510709204 2118 9 -0.6359280369153026 2118 10-11 -0.38288356130236423 2118 12-13 0.9661671961210416 2118 14-15 -0.26037299420416815 2118 16-17 0.3413602176470576 2118 18-19 -0.39409607113323375 2118 20-21 -2.5074274726979446 2118 22-23 -0.08343349396132282 2118 24-25 0.393423488067306 2118 26-27 0.9663262725105355 2118 28-29 0.014124656717079631 2118 30-31 -0.10404107172247024 2118 32-33 0.05384035313245761 2118 34-35 1.068257289582938 2118 36-37 0.49699117984066277 2118 38-39 -0.1122744902487085 2118 40-41 -1.1904438255117569 2118 42-43 0.06697981181030599 2118 44-45 0.8106759513688573 2118 46-47 -0.5491298665134394 2118 48-49 -0.646647354072762 2118 50-51 0.1231079292820354 2118 52-53 -0.8879920476083925 2118 54-55 0.6058890414441862 2118 56-57 1.4642469514021954 2118 58-59 1.1493927183935426 2118 60-61 -1.680697547630345 2118 62-63 0.5415953520472598 2118 64-65 -0.045836185103937055 2118 66-67 -1.6292502421701727 2118 68-69 1.5860653729305838 2118 70-71 -0.3656617566533171 2118 72-73 -1.2060133954987187 2118 74-75 0.5214508822821244 2118 76 -0.1995874752764557 2119 1 0.8337009165594829 2119 2 1.0240018548407583 2119 3 0.5745671644543364 2119 4 0.25458501365221764 2119 5 0.9248321504825228 2119 6 0.831979552851088 2119 7 -1.3165439993352663 2119 8 0.8589554160309092 2119 9 0.2374624890078607 2119 10-11 0.3355013696935316 2119 12-13 0.6288042718390514 2119 14-15 -0.30945169618104273 2119 16-17 -0.1404264291265349 2119 18-19 -0.699586559756888 2119 20-21 -0.6675729408068563 2119 22-23 0.7667940330808207 2119 24-25 0.31484086964135116 2119 26-27 0.5348790513244097 2119 28-29 0.7281604642515802 2119 30-31 0.08404546315258443 2119 32-33 0.6429153251578157 2119 34-35 0.8420431903662333 2119 36-37 -0.10818226290457744 2119 38-39 0.3829996611873199 2119 40-41 -0.08513983446364648 2119 42-43 -0.12023018445974287 2119 44-45 -1.0739454585528136 2119 46-47 -0.15031972104532798 2119 48-49 -0.5729658919317657 2119 50-51 1.0589490333476803 2119 52-53 -0.4530871613719114 2119 54-55 -0.5110635881716306 2119 56-57 0.8386482941847362 2119 58-59 1.5154688094043607 2119 60-61 0.7242856675878571 2119 62-63 0.304515904080084 2119 64-65 0.11568563511239915 2119 66-67 0.19875796372314625 2119 68-69 -0.5846358579534083 2119 70-71 -0.35409144706733997 2119 72-73 -1.9498254059425832 2119 74-75 0.3956246980225231 2119 76 1.1940119279312995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 22.0 13 167.0 14 496.0 15 797.0 16 996.0 17 1235.0 18 1266.0 19 1257.0 20 1207.0 21 1148.0 22 1096.0 23 1222.0 24 1351.0 25 1584.0 26 1868.0 27 2300.0 28 2662.0 29 3350.0 30 4028.0 31 5119.0 32 6669.0 33 8623.0 34 11976.0 35 18137.0 36 30107.0 37 37638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.23546581670235 22.932514577209297 12.572541838483833 28.25947776760452 2 17.00586361958914 19.94655768626218 39.22269453138882 23.824884162759865 3 16.702431556934517 25.49376050736028 29.657750502303077 28.146057433402127 4 12.165302133592117 19.987562019053346 39.334773041018 28.51236280633654 5 11.902874403728662 38.95411615160669 35.59244426827768 13.550565176386971 6 26.95351475472575 39.62385358719571 18.083594166450254 15.339037491628282 7 25.236800021868977 30.680808605442643 24.75568251711931 19.326708855569073 8 21.998824542459985 37.33307819526263 19.697114661782596 20.970982600494786 9 23.507100583628336 15.824255429657066 22.50454464688435 38.164099339830244 10-11 24.536309336686575 25.86450801634706 27.633844976285825 21.965337670680533 12-13 23.2928529447945 23.498558014296844 26.806582562224072 26.40200647868458 14-15 20.85856238809234 25.345803206538825 24.59200688872791 29.203627516640925 16-17 22.150540573787296 27.835107909735797 25.967702253871494 24.046649262605417 18-19 21.729221054358078 26.985293112638903 26.620354550797536 24.665131282205486 20-21 21.27988190752156 27.393627926684257 26.33537443789894 24.991115727895245 22-23 22.41843554802291 27.006136981807742 26.86125500594563 23.714172464223722 24-25 21.774667523201618 28.167243005344233 26.299837349479926 23.758252121974223 26-27 20.737941309131667 28.30939135902027 27.086437133523777 23.86623019832429 28-29 21.772275603788803 27.276765578229433 28.167243005344233 22.783715812637535 30-31 21.849500430545493 27.231319109385893 27.60821726829135 23.310963191777265 32-33 20.61492831075817 28.234900154449655 27.51664092505775 23.63353060973443 34-35 22.04324590298375 27.412763281986795 27.186214343315612 23.357776471713844 36-37 22.360346076568757 26.903284447056574 26.841777947869826 23.894591528504847 38-39 21.779793064800515 27.588056804669026 27.79478698249115 22.837363148039312 40-41 21.82728975028361 26.410890750789335 27.93625193062067 23.825567568306386 42-43 21.323961565272064 26.55679783497123 28.121454833727434 23.997785766029278 44-45 20.566748219728552 26.64222352828616 28.050038954116154 24.74098929786914 46-47 20.85001981876085 26.28446072468324 28.982887525115153 23.882631931440756 48-49 20.214794363271054 26.703046621926386 29.154422317291527 23.927736697511037 50-51 20.407856430162788 26.501441985703156 28.478875934557085 24.61182564957697 52-53 19.931181061465495 26.63812309500704 28.899512048439785 24.53118379508768 54-55 19.41145114333748 27.278132389322472 28.429329032434424 24.88108743490562 56-57 19.658160545630988 26.960348810190943 28.729002364583188 24.652488279594877 58-59 20.126976750543307 26.631972445088365 28.162117463745336 25.078933340622996 60-61 20.074012820688054 26.479231305441274 27.924975739103097 25.521780134767575 62-63 19.892568648087146 26.41567458961497 27.96051282752211 25.731243934775776 64-65 20.596818063775405 26.768311851618986 27.213550565176387 25.42131951942922 66-67 20.711630195590665 26.569782540355096 26.759085876740972 25.95950138731326 68-69 20.364460177958804 26.548938671186256 26.82571791752662 26.260883233328318 70-71 20.892049259871794 26.855787761573474 26.45155338080724 25.8006095977475 72-73 20.925536131651246 26.943605374301217 25.744228640159644 26.386629853887893 74-75 20.714022115003488 27.371075543649116 25.707324740647596 26.207577600699807 76 21.309268346021895 26.721840274455666 26.105408471495156 25.86348290802728 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 2.0 13 3.5 14 2.5 15 2.0 16 9.5 17 15.0 18 14.5 19 23.5 20 39.5 21 46.0 22 64.0 23 120.5 24 165.5 25 172.0 26 271.0 27 443.5 28 633.0 29 749.0 30 915.0 31 1280.5 32 1834.0 33 2188.0 34 2563.5 35 3346.0 36 4098.5 37 4444.0 38 5092.5 39 6486.0 40 7886.5 41 9047.0 42 9552.0 43 9777.5 44 10455.0 45 10936.5 46 10966.0 47 11003.5 48 10577.0 49 9715.5 50 9318.0 51 8509.5 52 6944.0 53 5685.0 54 5183.0 55 4810.5 56 3837.5 57 2806.5 58 2376.0 59 2021.5 60 1423.5 61 1026.0 62 872.0 63 712.5 64 496.5 65 378.0 66 264.5 67 213.0 68 177.0 69 113.5 70 77.5 71 69.0 72 58.0 73 38.0 74 26.5 75 24.0 76 19.5 77 14.5 78 12.0 79 10.0 80 8.0 81 5.0 82 2.5 83 1.0 84 4.0 85 8.0 86 7.0 87 5.0 88 5.0 89 4.5 90 3.0 91 1.0 92 0.0 93 1.0 94 2.5 95 2.0 96 1.0 97 0.5 98 1.5 99 5.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7278269070431775 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 146326.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.35248691278379 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04077126239375 35.53367139127701 2 8.83112759601091 8.363517078304609 3 4.725136746092453 6.7124092779137 4 2.8763584407337386 5.44810901685278 5 1.75641155161714 4.1585227505706435 6 1.2253027176030826 3.4812678539699027 7 0.8832570826538122 2.927709361289176 8 0.6566698898815108 2.4875961893306724 9 0.5671895971943598 2.4172054180391727 >10 3.3959214305300987 27.262414061752526 >50 0.04185368528915123 1.2075776006998071 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007517461011713571 0.0 2 0.0 0.0 0.0 0.012984705383868896 0.0 3 0.0 0.0 0.0 0.017768544209504803 0.0 4 0.0 0.0 0.0 0.03280346623293195 0.0 5 0.0 0.0 0.0 0.034853682872490195 0.0 6 0.0 0.0 0.0 0.03690389951204844 0.0 7 0.0 0.0 0.0 0.03827071060508727 0.0 8 0.0 0.0 0.0 0.043737954977242595 0.0 9 0.0 0.0 0.0 0.07654142121017454 0.0 10 0.0 0.0 0.0 0.09977720979183467 0.0 11 0.0 0.0 0.0 0.1141287262687424 0.0 12 0.0 0.0 0.0 0.12027937618741713 0.0 13 0.0 0.0 0.0 0.12369640392001421 0.0 14 0.0 0.0 0.0 0.1312138649317278 0.0 15 0.0 0.0 0.0 0.13463089266432487 0.0 16 0.0 0.0 0.0 0.14214835367603842 0.0 17 0.0 0.0 0.0 0.1503492202342714 0.0 18 0.0 0.0 0.0 0.1585500867925044 0.0 19 0.0 0.0 0.0 0.1633339256181403 0.0 20 0.0 0.0 0.0 0.16948457553681506 0.0 21 0.0 0.0 0.0 0.17495181990897038 0.0 22 0.0 0.0 0.0 0.18383609201372278 0.0 23 0.0 0.0 0.0 0.1934037696649946 0.0 24 0.0 0.0 0.0 0.202288041769747 0.0 25 0.0 0.0 0.0 0.21048890832797998 0.0 26 0.0 0.0 0.0 0.22552383035140713 0.0 27 0.0 0.0 0.0 0.25149324111914495 0.0 28 0.0 0.0 0.0 0.27746265188688274 0.0 29 0.0 0.0 0.0 0.31094952366633405 0.0 30 0.0 0.0 0.0 0.3444363954457854 0.0 31 0.0 0.0 0.0 0.3820237005043533 0.0 32 0.0 0.0 0.0 0.42302803329551825 0.0 33 0.0 0.0 0.0 0.47770047701707147 0.0 34 0.0 0.0 0.0 0.5439908150294548 0.0 35 0.0 0.0 0.0 0.6273662917048235 0.0 36 0.0 0.0 0.0 0.7496958845317988 0.0 37 0.0 0.0 0.0 1.0203244809534875 0.0 38 0.0 0.0 0.0 1.3777455817831417 0.0 39 0.0 0.0 0.0 1.8342604868581114 0.0 40 0.0 0.0 0.0 2.4172054180391727 0.0 41 0.0 0.0 0.0 3.017918893429739 0.0 42 0.0 0.0 0.0 3.639134535215888 0.0 43 0.0 0.0 0.0 4.280852343397619 0.0 44 0.0 0.0 0.0 4.898650957451171 0.0 45 0.0 0.0 0.0 5.534901521260746 0.0 46 0.0 0.0 0.0 6.242909667454861 0.0 47 0.0 0.0 0.0 6.9208479696021215 0.0 48 0.0 0.0 0.0 7.5899019996446295 0.0 49 0.0 0.0 0.0 8.21863510244249 0.0 50 0.0 0.0 0.0 8.941678170660033 0.0 51 0.0 0.0 0.0 9.564260623539221 0.0 52 0.0 0.0 0.0 10.24219892568648 0.0 53 0.0 0.0 0.0 10.853846889821357 0.0 54 0.0 0.0 0.0 11.486680425898337 0.0 55 0.0 0.0 0.0 12.00811885789265 0.0 56 0.0 0.0 0.0 12.576028867050285 0.0 57 0.0 0.0 0.0 13.087216215846809 0.0 58 0.0 0.0 0.0 13.595669942457253 0.0 59 0.0 0.0 0.0 14.143761190765824 0.0 60 0.0 0.0 0.0 14.67476730041141 0.0 61 0.0 0.0 0.0 15.289148886732365 0.0 62 0.0 0.0 0.0 15.813320940912757 0.0 63 0.0 0.0 0.0 16.360045378128287 0.0 64 0.0 0.0 0.0 16.90745322089034 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCATT 30 8.348818E-6 58.327354 33 ATTCCCG 25 2.3853302E-4 55.994263 31 TTCCCGG 25 2.3853302E-4 55.994263 32 TCCCGGA 25 2.3853302E-4 55.994263 33 CCCGGAC 25 2.3853302E-4 55.994263 34 AGCGTGG 20 0.0069427923 52.494617 58 GAGTATC 20 0.0069427923 52.494617 7 CAACCGT 20 0.0069427923 52.494617 6 GGATCCG 20 0.0069427923 52.494617 60 TGTATAA 20 0.0069427923 52.494617 2 CCAGAGG 20 0.0069427923 52.494617 47 GACGGTT 20 0.0069427923 52.494617 11 CACAGTG 20 0.0069427923 52.494617 7 GGACGTC 20 0.0069427923 52.494617 37 CAGACGG 20 0.0069427923 52.494617 9 CGGACGT 20 0.0069427923 52.494617 36 GCAATTC 30 5.8654655E-4 46.661884 28 TGCAATT 30 5.8654655E-4 46.661884 27 GAGGCGC 30 5.8654655E-4 46.661884 29 GATGCAA 35 0.0012528121 39.9959 25 >>END_MODULE