FastQCFastQC Report
Wed 25 May 2016
SRR1780670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146326
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT14350.9806869592553613No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5610.3833905115973921TruSeq Adapter, Index 12 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC3440.23509150800267892No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2930.20023782513018876No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGA252.384122E-456.0000045
CGTGATA200.006939977452.524
AGGGTAC200.006939977452.556
GAATAGC407.439121E-752.521
AGCGGTC200.006939977452.556
CTACGTG200.006939977452.568
CGCTATT200.006939977452.532
GAGCGGT200.006939977452.555
TGTTCCG200.006939977452.55
GGTATGC200.006939977452.530
GTTCCGT200.006939977452.56
TAGCTCG200.006939977452.59
CGCCATT506.151822E-849.00000417
CGAGTGA451.6756494E-646.66666464
GAGTCCC305.862503E-446.66666442
GTCTCGA451.6756494E-646.66666460
GTCAGTA305.862503E-446.66666415
ACGATGT305.862503E-446.6666648
AGGTTAC305.862503E-446.66666451
ACGTCTC451.6756494E-646.66666458
Produced by FastQC (version 0.11.5)