##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780670_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 146326 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77084728619658 34.0 34.0 34.0 31.0 34.0 2 33.30901548596968 34.0 34.0 34.0 33.0 34.0 3 33.4534464141711 34.0 34.0 34.0 34.0 34.0 4 33.50861774394161 34.0 34.0 34.0 34.0 34.0 5 33.53135464647431 34.0 34.0 34.0 34.0 34.0 6 37.24424914232604 38.0 37.0 38.0 36.0 38.0 7 36.93677815289149 38.0 37.0 38.0 35.0 38.0 8 36.912824788485985 38.0 37.0 38.0 35.0 38.0 9 36.72593387367932 38.0 37.0 38.0 34.0 38.0 10-11 36.449390402252504 38.0 37.0 38.0 34.0 38.0 12-13 36.32152522449872 38.0 37.0 38.0 34.0 38.0 14-15 36.581571286032556 38.0 37.0 38.0 34.0 38.0 16-17 36.709497970285526 38.0 37.0 38.0 34.0 38.0 18-19 36.722930306302366 38.0 37.0 38.0 34.0 38.0 20-21 36.462053907029514 38.0 37.0 38.0 34.0 38.0 22-23 36.53974344955783 38.0 37.0 38.0 34.0 38.0 24-25 35.90406694640734 38.0 36.5 38.0 32.5 38.0 26-27 36.125924306001664 38.0 37.0 38.0 34.0 38.0 28-29 35.994860790290176 38.0 37.0 38.0 33.0 38.0 30-31 35.696872052813575 38.0 36.5 38.0 31.5 38.0 32-33 36.197982586826676 38.0 37.0 38.0 34.0 38.0 34-35 36.251732433060425 38.0 37.0 38.0 33.5 38.0 36-37 36.39457102633845 38.0 37.0 38.0 34.0 38.0 38-39 36.0632731025245 38.0 37.0 38.0 32.5 38.0 40-41 35.56781774940885 38.0 36.0 38.0 31.0 38.0 42-43 35.95467996118256 38.0 36.5 38.0 32.5 38.0 44-45 36.13462064158112 38.0 37.0 38.0 34.0 38.0 46-47 36.41747878025778 38.0 37.0 38.0 34.0 38.0 48-49 36.22705124174788 38.0 37.0 38.0 34.0 38.0 50-51 36.36113882700272 38.0 37.0 38.0 34.0 38.0 52-53 36.294568292716264 38.0 37.0 38.0 34.0 38.0 54-55 36.448840260787556 38.0 37.0 38.0 34.0 38.0 56-57 36.51708855569072 38.0 37.0 38.0 34.0 38.0 58-59 36.5714808031382 38.0 37.0 38.0 34.0 38.0 60-61 36.26936087913289 38.0 37.0 38.0 33.5 38.0 62-63 36.509328485709986 38.0 37.0 38.0 34.0 38.0 64-65 36.532143979880544 38.0 37.0 38.0 34.0 38.0 66-67 35.95505241720542 38.0 37.0 38.0 33.0 38.0 68-69 35.44156882577259 38.0 36.0 38.0 31.0 38.0 70-71 35.46529324932001 38.0 36.0 38.0 31.0 38.0 72-73 35.24030247529489 38.0 36.0 38.0 29.0 38.0 74-75 35.83626969916487 38.0 37.0 38.0 32.5 38.0 76 35.283743148859394 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5015253037015484 1101 2 0.4080411614593089 1101 3 0.10211614316521889 1101 4 0.23777420500336888 1101 5 0.1211255020120845 1101 6 -1.0067885588231604 1101 7 0.21608983730812525 1101 8 0.3624906645256445 1101 9 0.04134360866444098 1101 10-11 0.17205121927288047 1101 12-13 0.6631048336660328 1101 14-15 0.5932232869222602 1101 16-17 0.20315147253068488 1101 18-19 -0.24785758009793568 1101 20-21 -0.038525297416818205 1101 22-23 0.6747307905292885 1101 24-25 -1.2800648241592185 1101 26-27 -0.2913878627738953 1101 28-29 0.5590399477814643 1101 30-31 1.1781115514765546 1101 32-33 0.3765567322695631 1101 34-35 0.42470148169208954 1101 36-37 0.2638556700784136 1101 38-39 0.1919077626763368 1101 40-41 1.3156595906117943 1101 42-43 0.6697697081137477 1101 44-45 0.4788081818686223 1101 46-47 0.5330148510154871 1101 48-49 -1.0548305143857704 1101 50-51 -1.1977920357807719 1101 52-53 -1.1838766877207334 1101 54-55 -0.9751343978291018 1101 56-57 -1.1240769807561861 1101 58-59 -0.9053686047121516 1101 60-61 -1.4935237609427858 1101 62-63 -0.6120366239334203 1101 64-65 -1.280401599494759 1101 66-67 -1.5382119142401862 1101 68-69 -1.1865564009176097 1101 70-71 -0.8553925363574209 1101 72-73 -1.5446040279515074 1101 74-75 -0.762540239390809 1101 76 -1.0518587490404698 1102 1 -0.17518375830163535 1102 2 0.053271686101595606 1102 3 0.23780771709208892 1102 4 0.26639105714963307 1102 5 0.2497423541583501 1102 6 0.24782193402262465 1102 7 0.07093880392179841 1102 8 0.45581339902484785 1102 9 0.475127392448222 1102 10-11 0.363505909256979 1102 12-13 0.6151716063210415 1102 14-15 0.04262710250254287 1102 16-17 0.2532309637866419 1102 18-19 -0.20254756419968345 1102 20-21 0.5916018885927272 1102 22-23 0.6084350830571097 1102 24-25 -4.200136366289577 1102 26-27 -0.9561732363827886 1102 28-29 0.3126965455557098 1102 30-31 1.5423404862937247 1102 32-33 0.4164215971344305 1102 34-35 0.7082070460800054 1102 36-37 -2.2320107846115675 1102 38-39 -0.08015106085306911 1102 40-41 1.2392287480044857 1102 42-43 0.9994199465875155 1102 44-45 1.212194509372587 1102 46-47 1.0161627047515793 1102 48-49 -3.0071357608086657 1102 50-51 -0.2436982360987443 1102 52-53 0.09931091164333594 1102 54-55 0.47415018086724103 1102 56-57 0.6864953562867342 1102 58-59 0.06820055268053693 1102 60-61 -5.4693186735024 1102 62-63 -2.3811940166202277 1102 64-65 0.020274076180911038 1102 66-67 0.8950551763798487 1102 68-69 -0.715769437483587 1102 70-71 -1.1175149528916037 1102 72-73 -5.616861579620821 1102 74-75 -2.1484305414575857 1102 76 -0.26815445651264724 1103 1 0.7538208560688346 1103 2 0.41385177838900233 1103 3 0.04393823785675721 1103 4 0.38339830830322086 1103 5 0.29113984921438174 1103 6 0.2423506090391072 1103 7 0.6681701816409813 1103 8 0.5118450404223438 1103 9 0.39866068183517456 1103 10-11 0.395114346962977 1103 12-13 -0.34636431984903027 1103 14-15 -0.33608087376640583 1103 16-17 0.16957901915908735 1103 18-19 0.6999903395577363 1103 20-21 -0.2076070764698983 1103 22-23 0.5473276341447786 1103 24-25 -2.3670447380759967 1103 26-27 -0.2932529990723154 1103 28-29 -0.09260998048252134 1103 30-31 0.9027887394248992 1103 32-33 1.1649713664159052 1103 34-35 0.39159531239510414 1103 36-37 -0.6599606857322016 1103 38-39 0.11740991475668494 1103 40-41 0.08276862934923912 1103 42-43 -0.1637310092463693 1103 44-45 1.0966704487265773 1103 46-47 0.8296432584760325 1103 48-49 -1.7690342446583642 1103 50-51 -0.4651880185641346 1103 52-53 0.34413622476133554 1103 54-55 -0.0034701223628204048 1103 56-57 0.4924940378951703 1103 58-59 -0.28825956597469826 1103 60-61 -3.887677276007345 1103 62-63 -1.1357095077210886 1103 64-65 0.3348423029442671 1103 66-67 0.11110659365077424 1103 68-69 0.7165641155816687 1103 70-71 0.6040407491519062 1103 72-73 -4.711390254637301 1103 74-75 -1.8698214445558037 1103 76 -3.178042393229852 1104 1 -0.06736014521486311 1104 2 0.1298396416169183 1104 3 0.34119579416437773 1104 4 -0.15043294388489414 1104 5 -0.16947399137378483 1104 6 0.3289418098793462 1104 7 0.6332302820027493 1104 8 0.5729606898563517 1104 9 0.7195732737362093 1104 10-11 -0.7968975564096468 1104 12-13 0.3073350616423909 1104 14-15 0.4741478577075142 1104 16-17 0.5710377658039718 1104 18-19 0.7092913081655112 1104 20-21 -0.0447876109546641 1104 22-23 -0.0012894740358717627 1104 24-25 0.2349858372536815 1104 26-27 0.8140582391118372 1104 28-29 1.0399434096446782 1104 30-31 0.3752125928500405 1104 32-33 0.4149538614561692 1104 34-35 0.2570755317905977 1104 36-37 0.9927467480406946 1104 38-39 1.2772652070895134 1104 40-41 1.489998177721283 1104 42-43 0.4241322186682126 1104 44-45 0.668084849473459 1104 46-47 0.7114556053076413 1104 48-49 -0.116116751368601 1104 50-51 0.05588795296113602 1104 52-53 0.035325395026241324 1104 54-55 0.20120953687396081 1104 56-57 0.11293399588326736 1104 58-59 -0.429594610904104 1104 60-61 0.4296467991472852 1104 62-63 0.26971119481130046 1104 64-65 0.3977084483521338 1104 66-67 -0.2066259305644209 1104 68-69 1.2096088115788675 1104 70-71 1.1121630504706133 1104 72-73 1.0429276354315533 1104 74-75 0.01801844341245129 1104 76 -0.06591729111758582 1105 1 0.8064365793757133 1105 2 0.3524242828788928 1105 3 0.3357405668064146 1105 4 -0.0864817681697545 1105 5 -0.35966253766935097 1105 6 0.18479310947360972 1105 7 0.6412540122417525 1105 8 -0.7227126329453597 1105 9 0.6690158176905712 1105 10-11 0.4146004717529692 1105 12-13 -0.29731154191032516 1105 14-15 0.16033590394056318 1105 16-17 0.3622474624705987 1105 18-19 0.5437450467010052 1105 20-21 0.26823731261531947 1105 22-23 0.31349703336846346 1105 24-25 0.5854898775314368 1105 26-27 0.5021499323916814 1105 28-29 0.8355314945189249 1105 30-31 0.9723270048788137 1105 32-33 0.8194902239560236 1105 34-35 -0.5613506351166322 1105 36-37 0.26808230134849964 1105 38-39 -0.13466412498608804 1105 40-41 0.275744123237196 1105 42-43 0.35616065404081354 1105 44-45 0.6019743129291228 1105 46-47 0.837681610831055 1105 48-49 -0.17265222739401764 1105 50-51 0.7429198546761242 1105 52-53 0.27527868464198235 1105 54-55 0.6907451606451218 1105 56-57 0.6416279235333207 1105 58-59 0.39497721057358604 1105 60-61 0.5982360546223973 1105 62-63 -0.2572580854396449 1105 64-65 0.6573062389850506 1105 66-67 0.7454788779911965 1105 68-69 0.1459048258533855 1105 70-71 0.9084784989925865 1105 72-73 1.0967610691562228 1105 74-75 0.01892196544361724 1105 76 0.7232495365540572 1106 1 -0.7029510604875426 1106 2 0.058390758662525855 1106 3 0.2500641595810933 1106 4 0.1470461308279596 1106 5 0.024454275381899038 1106 6 0.1750095867916741 1106 7 -1.0263026235521906 1106 8 0.27910413161790615 1106 9 -0.8766150476663057 1106 10-11 0.303222369903672 1106 12-13 0.9294219031294801 1106 14-15 -0.3897110496030436 1106 16-17 0.0873359990544671 1106 18-19 0.4311209880735234 1106 20-21 0.1065575419204734 1106 22-23 0.4773679694318531 1106 24-25 -0.34893167322442054 1106 26-27 0.7961558368765154 1106 28-29 0.7275229999170421 1106 30-31 0.8395190602164817 1106 32-33 0.19264200358739458 1106 34-35 0.7043450445435866 1106 36-37 0.8924846410466429 1106 38-39 -0.1658099755817517 1106 40-41 0.6478187333386529 1106 42-43 0.8053526234504247 1106 44-45 0.9060767637199589 1106 46-47 0.7985567266804807 1106 48-49 1.1787536169411723 1106 50-51 0.04688420680928118 1106 52-53 0.48396416955240085 1106 54-55 -0.07478480062587778 1106 56-57 0.09055290220474888 1106 58-59 0.6134830444746626 1106 60-61 0.7201219298916897 1106 62-63 0.6775286595443077 1106 64-65 -0.6475204625438522 1106 66-67 0.13409770728284087 1106 68-69 1.233640437507681 1106 70-71 0.16538189744710508 1106 72-73 0.1466484420287344 1106 74-75 -0.35389530855025697 1106 76 0.2618895409732147 1107 1 0.8192699370999321 1107 2 -0.2320619888055404 1107 3 0.2012379719915387 1107 4 0.25137888858296265 1107 5 0.1802278159234305 1107 6 0.28569819891311 1107 7 0.6707594776761212 1107 8 0.035114026666711595 1107 9 -0.1660023628995404 1107 10-11 0.6838786516676336 1107 12-13 0.1397008749261417 1107 14-15 0.2640693978784938 1107 16-17 0.32174639865278465 1107 18-19 0.5680749540531664 1107 20-21 0.770089143620261 1107 22-23 0.07935861322233961 1107 24-25 0.41488220678586885 1107 26-27 -0.10596829210202685 1107 28-29 0.7814681607728176 1107 30-31 0.56679290079353 1107 32-33 0.9190922772932666 1107 34-35 0.3185054961888838 1107 36-37 1.0201283213189996 1107 38-39 0.33833235146920515 1107 40-41 0.38509783984513746 1107 42-43 0.7890894739347658 1107 44-45 0.5943643569452846 1107 46-47 0.9010800865883866 1107 48-49 0.8651583342342448 1107 50-51 0.8694438159058677 1107 52-53 0.6116139729985264 1107 54-55 0.6454259590150571 1107 56-57 0.7798538806879094 1107 58-59 0.5728374170330426 1107 60-61 1.1033468830494115 1107 62-63 0.7859700668945635 1107 64-65 0.8263519550076097 1107 66-67 0.20833812757748404 1107 68-69 1.1659456899661365 1107 70-71 0.5094736470827783 1107 72-73 1.4063995962469917 1107 74-75 -0.01158540247974571 1107 76 1.2373662300506965 1108 1 -0.45258525618505985 1108 2 0.3160851019270581 1108 3 0.22865760312269856 1108 4 0.3340888754498579 1108 5 0.2258739073983307 1108 6 -0.3344639663355622 1108 7 -0.10984921301079709 1108 8 -0.6716695055664843 1108 9 -0.26769253591387354 1108 10-11 0.3030044439362385 1108 12-13 0.8701211341621331 1108 14-15 0.45394263490241826 1108 16-17 0.0021238325590289264 1108 18-19 0.38927920232588065 1108 20-21 0.8256136111586656 1108 22-23 0.08057770760937188 1108 24-25 0.773390172297205 1108 26-27 -0.1900058641913276 1108 28-29 -0.6587134186253394 1108 30-31 0.18392303269554588 1108 32-33 0.8431229973298073 1108 34-35 0.6042018360506987 1108 36-37 0.9965629698657992 1108 38-39 1.0710537584240356 1108 40-41 0.9278318088966984 1108 42-43 -0.2261906037218253 1108 44-45 0.7267174660642226 1108 46-47 0.6713564527164095 1108 48-49 0.834007189370432 1108 50-51 0.7059110117032787 1108 52-53 -0.10689227949961122 1108 54-55 -0.1339579272408713 1108 56-57 0.2790059391587647 1108 58-59 0.4170445774281717 1108 60-61 1.022475530339996 1108 62-63 0.8404438863429746 1108 64-65 0.5452008101437968 1108 66-67 0.7250942515996428 1108 68-69 1.4826675537244896 1108 70-71 -0.1366292479095108 1108 72-73 -0.09361182384094491 1108 74-75 -0.6889222379733795 1108 76 0.8539881680396135 1109 1 -0.9220944988293951 1109 2 0.2003996651444666 1109 3 0.1524777062301652 1109 4 0.12428512142667358 1109 5 -0.11694986998068657 1109 6 -0.1316043007227563 1109 7 -0.21004004726982117 1109 8 0.30708807795910076 1109 9 0.4970173675296863 1109 10-11 0.42351485437230707 1109 12-13 0.18221205102106097 1109 14-15 0.6356339391264001 1109 16-17 0.671507751212367 1109 18-19 0.6726680769734088 1109 20-21 0.6111757177410269 1109 22-23 0.35814756149299143 1109 24-25 -0.17808665700582793 1109 26-27 0.31715754120330786 1109 28-29 -0.6836870721045045 1109 30-31 0.29556392249588015 1109 32-33 0.6151501128842156 1109 34-35 -0.18641310598695782 1109 36-37 0.8820937454504119 1109 38-39 1.0242224616055609 1109 40-41 0.6913340447906293 1109 42-43 0.835810592552626 1109 44-45 0.5217785615098407 1109 46-47 0.7088596570229981 1109 48-49 0.593097451126674 1109 50-51 -0.06255006665269747 1109 52-53 -0.08555587003196052 1109 54-55 -0.5828940974536323 1109 56-57 0.10536954962901746 1109 58-59 0.05600324899387488 1109 60-61 0.7514595515311413 1109 62-63 0.40227988281111493 1109 64-65 0.5974352799378622 1109 66-67 0.40541553674020037 1109 68-69 0.3170974719790607 1109 70-71 0.47051137130493714 1109 72-73 0.12237552982905697 1109 74-75 0.008878895639092832 1109 76 0.36134525596578726 1110 1 -0.36269658758661194 1110 2 -0.21976970972460919 1110 3 -0.10789874886754092 1110 4 0.07299386248661222 1110 5 0.3753325012674793 1110 6 -0.07123383060275756 1110 7 0.6064844761763268 1110 8 0.786466837273224 1110 9 1.0508372795505139 1110 10-11 -0.28165317387678357 1110 12-13 0.5978777301664024 1110 14-15 0.8713133837204694 1110 16-17 0.6024441009744592 1110 18-19 -0.17511000005325883 1110 20-21 0.9104694903717174 1110 22-23 -0.44354917968664864 1110 24-25 -0.6780357846953322 1110 26-27 0.8142338795939423 1110 28-29 0.17776667898027654 1110 30-31 -0.055469982402819085 1110 32-33 0.7249744537885547 1110 34-35 0.7164177884679646 1110 36-37 1.3403634883956173 1110 38-39 0.8521274201595901 1110 40-41 -1.0630634175788245 1110 42-43 0.15338847207502937 1110 44-45 0.860193140915527 1110 46-47 0.8095598994146016 1110 48-49 1.1327718683394679 1110 50-51 -0.6032090126981231 1110 52-53 0.5201627761660816 1110 54-55 -0.4099072943294786 1110 56-57 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-1.1460632241902644 2116 36-37 0.4781693955686066 2116 38-39 -3.730151060853075 2116 40-41 -0.8676541348783999 2116 42-43 -1.0892106840431168 2116 44-45 -0.018760445582366003 2116 46-47 -0.5054949529060764 2116 48-49 0.18207144639854533 2116 50-51 -0.5533018397023497 2116 52-53 0.20210370443612646 2116 54-55 -0.1446245939075368 2116 56-57 0.054315176106555896 2116 58-59 -1.6506823302023435 2116 60-61 -1.5743276825114094 2116 62-63 0.3250221995959919 2116 64-65 -1.6520682661614217 2116 66-67 -0.7639177965931268 2116 68-69 -1.1475171852313366 2116 70-71 1.007097659721012 2116 72-73 -0.10644716520640429 2116 74-75 -0.1351872982143405 2116 76 -0.8235058078639952 2117 1 0.9754291888630391 2117 2 0.5745491970342798 2117 3 0.3422238042667942 2117 4 0.14058275547925803 2117 5 0.24064492781953817 2117 6 0.40599814737125683 2117 7 0.7238601602914301 2117 8 0.578811463402225 2117 9 -0.499107319717524 2117 10-11 -0.3386017687127705 2117 12-13 -0.661891265569416 2117 14-15 0.43984912558664035 2117 16-17 -0.1933924500781501 2117 18-19 -0.5776282151424041 2117 20-21 0.3242458057194071 2117 22-23 0.5150412913874547 2117 24-25 -1.1722203866494638 2117 26-27 -1.1025206447991707 2117 28-29 -0.5441820837367146 2117 30-31 -0.006566245371629975 2117 32-33 -1.470724434839191 2117 34-35 0.5655229827062911 2117 36-37 -0.5594787034588009 2117 38-39 0.4804590187225273 2117 40-41 0.6219515238307096 2117 42-43 0.6874011638790805 2117 44-45 -0.1492149106575127 2117 46-47 -0.4996735559122669 2117 48-49 -0.06274906642196498 2117 50-51 0.21825961034186037 2117 52-53 0.736621829988735 2117 54-55 -0.03740620310293963 2117 56-57 0.47563436266712245 2117 58-59 -0.5387318439070299 2117 60-61 -0.12165217411176599 2117 62-63 0.11764819429095041 2117 64-65 -0.4629612811923778 2117 66-67 -0.13481434831727057 2117 68-69 0.9161468289154371 2117 70-71 0.09739651029572371 2117 72-73 1.0904794484098659 2117 74-75 0.956483789318817 2117 76 0.8237302664065567 2118 1 -0.4567376359390636 2118 2 0.2318547077655566 2118 3 -0.6280097975504901 2118 4 0.16173365823635777 2118 5 0.2077481406236643 2118 6 0.08788967800681746 2118 7 0.6878606866400219 2118 8 0.3438452951507429 2118 9 -1.4005623965574898 2118 10-11 -0.7359083875463526 2118 12-13 -0.7514397831362984 2118 14-15 0.5598912334742536 2118 16-17 -2.8832484660348214 2118 18-19 -0.5872063744459624 2118 20-21 -0.6761385672678344 2118 22-23 0.6598252460660703 2118 24-25 0.5916637889570566 2118 26-27 -1.719041065046973 2118 28-29 -0.2624851635685559 2118 30-31 -0.5821249713310053 2118 32-33 -0.08969653143800826 2118 34-35 -0.30514416413804923 2118 36-37 -3.0930403520380096 2118 38-39 -0.12806228800711494 2118 40-41 -0.07829295124044933 2118 42-43 0.4894594639116292 2118 44-45 -2.361385336792111 2118 46-47 -0.0517647527320122 2118 48-49 0.2429348058415357 2118 50-51 -0.8665794724264586 2118 52-53 -1.1672087411687428 2118 54-55 -0.8947934363792456 2118 56-57 0.21971813520141836 2118 58-59 -0.9696866818124406 2118 60-61 -0.8389891272459593 2118 62-63 -1.2486592625450044 2118 64-65 0.024624510165814684 2118 66-67 0.3018472164617023 2118 68-69 -1.2623857666064495 2118 70-71 -0.29271783201092916 2118 72-73 1.256386603287936 2118 74-75 0.2727500386002859 2118 76 0.15307904853286658 2119 1 0.487827824709953 2119 2 -0.7950680822381742 2119 3 0.11278841207278134 2119 4 -0.3407131899546201 2119 5 -0.12021903580303928 2119 6 -0.7504020042013124 2119 7 -0.8588681458851539 2119 8 -0.09129084807939591 2119 9 -0.4315135341927032 2119 10-11 -0.5114361756851054 2119 12-13 -0.42950020835077396 2119 14-15 -0.054129654254211346 2119 16-17 -0.4806300400743666 2119 18-19 -0.240462622114741 2119 20-21 -0.9860042890134508 2119 22-23 -0.3186305540085286 2119 24-25 0.5061956800424667 2119 26-27 0.13911633890678132 2119 28-29 -0.3863382034790419 2119 30-31 0.789792223745458 2119 32-33 0.13441387512670389 2119 34-35 0.4422224900954532 2119 36-37 -0.24849727109805997 2119 38-39 -1.271579632281643 2119 40-41 -1.2741303253545908 2119 42-43 0.12640836357593344 2119 44-45 -0.0037128265347448064 2119 46-47 0.007552666141542375 2119 48-49 -0.8551666488395568 2119 50-51 0.3731743507738443 2119 52-53 -0.03694391461149138 2119 54-55 -0.05681507009801123 2119 56-57 -1.5670181572267765 2119 58-59 -0.20372994924996135 2119 60-61 0.14214850796477663 2119 62-63 -0.4935492289754393 2119 64-65 -0.038258742351899855 2119 66-67 0.4235107748354423 2119 68-69 0.4274351957210456 2119 70-71 -0.0372832926599429 2119 72-73 0.6313623586031127 2119 74-75 -0.04233015535719886 2119 76 0.24906562070743377 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 6.0 22 6.0 23 22.0 24 49.0 25 77.0 26 192.0 27 343.0 28 655.0 29 1042.0 30 1882.0 31 3015.0 32 4869.0 33 7609.0 34 11745.0 35 18985.0 36 34070.0 37 61756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.90868335087408 23.91372688380739 11.513333242212594 27.66425652310594 2 17.476730041141014 19.215997157032927 39.391495701379114 23.91577710044695 3 17.195166955975015 25.847081174910812 30.410180009020955 26.547571860093218 4 12.194005166545931 19.5987042630838 40.41045337123956 27.796837199130707 5 12.075092601451553 37.853833221710424 37.53058239820675 12.540491778631274 6 26.922078099585857 41.237374082528056 18.080860544264176 13.759687273621912 7 25.6529939996993 30.338422426636413 24.573213236198622 19.43537033746566 8 22.67402922242117 36.83624236294302 20.439976490849197 20.049751923786612 9 22.88588494184219 14.947446113472656 23.298661891939915 38.86800705274524 10-11 24.610458838483932 26.004264450610282 28.170318330303566 21.214958380602216 12-13 23.511542719680715 23.440126840069432 27.236102948211528 25.812227492038325 14-15 20.987042630837994 24.91594111777811 24.430039774202807 29.66697647718109 16-17 22.5923622596121 27.882604595218897 26.020324480953487 23.504708664215517 18-19 22.608080587182044 27.203299481978593 26.584817462378524 23.603802468460835 20-21 21.215641786148737 27.680999958995667 26.438910378196628 24.66444787665897 22-23 21.956111695802523 27.676216120170032 27.126074655221906 23.241597528805542 24-25 22.39007421784235 28.220548637972747 26.571491054221397 22.81788608996351 26-27 21.684116288287797 28.396525566201497 27.122999330262566 22.796358815248144 28-29 21.607574867077624 26.90909339420199 28.57933654989544 22.90399518882495 30-31 21.805420772794992 27.499897489168024 27.48759618933067 23.207085548706313 32-33 21.008911608326613 28.211322663094734 28.284447056572308 22.49531867200634 34-35 22.399300192720364 27.89148886732365 27.896956111695804 21.812254828260187 36-37 22.486092697128328 28.087967961947978 26.688011699902958 22.737927641020732 38-39 21.908273307546164 27.25865533124667 27.84228366797425 22.990787693232917 40-41 21.217692002788294 26.37569536514358 28.80383527192707 23.602777360141054 42-43 22.248950972486092 26.212019736752186 28.55644246408704 22.982586826674687 44-45 20.50045788171617 26.899184013777454 29.061137460191627 23.53922064431475 46-47 21.556319451088665 25.939340923690935 28.788458647130383 23.715880978090016 48-49 21.079302379618113 26.125227232344216 29.014665883028307 23.78080450500936 50-51 20.421182838319915 26.127277448983776 29.16125637275672 24.290283339939585 52-53 19.532413925071417 26.594385140029797 30.390702950945148 23.48249798395364 54-55 18.90538933613985 26.868772466957342 30.010729467080356 24.21510872982245 56-57 20.1355193198748 26.33605784344546 28.830146385468066 24.69827645121168 58-59 19.535830952804012 26.606344737093885 29.156472533931083 24.701351776171016 60-61 20.35865123081339 27.31196096387518 27.827248745950822 24.502139059360605 62-63 18.952202616076434 28.44675587387067 27.525866899935757 25.075174610117134 64-65 19.42648606536091 28.832880007654143 26.50656752730205 25.2340663996829 66-67 19.359512321802004 28.669546082036 26.269767505433073 25.70117409072892 68-69 19.3663463772672 28.838347252026296 26.339474871178055 25.455831499528454 70-71 19.941090441890026 28.549950111395106 25.93319027377226 25.575769172942607 72-73 19.718300233724698 27.732938780531143 25.917813648975574 26.630947336768585 74-75 19.725134289189892 27.938985552806745 25.93387367931878 26.40200647868458 76 19.687546984131323 27.88636332572475 26.324781651927886 26.101308038216036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 2.5 15 5.5 16 8.0 17 10.0 18 17.5 19 23.0 20 35.5 21 50.0 22 61.0 23 96.0 24 126.5 25 133.0 26 262.5 27 503.5 28 729.0 29 843.0 30 931.5 31 1267.0 32 1899.5 33 2285.0 34 2602.5 35 3308.5 36 4125.0 37 4553.0 38 5447.0 39 7305.5 40 8907.5 41 9847.0 42 10149.0 43 10293.5 44 10920.5 45 11526.5 46 11650.0 47 11431.0 48 10578.5 49 9488.5 50 9032.0 51 8180.0 52 6455.5 53 5115.5 54 4648.0 55 4286.0 56 3329.0 57 2392.5 58 2051.0 59 1622.0 60 1021.5 61 715.0 62 580.0 63 473.5 64 334.5 65 250.5 66 168.0 67 137.0 68 106.5 69 62.5 70 34.0 71 19.0 72 13.5 73 6.0 74 4.5 75 5.0 76 3.0 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 146326.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.93007394448013 #Duplication Level Percentage of deduplicated Percentage of total 1 58.830957578870766 18.784768257179174 2 13.336329780403236 8.516599920724957 3 7.705149608321562 7.380779902409689 4 4.704421899747442 6.008501564998701 5 3.047814733958307 4.865847491218238 6 2.039724326869569 3.9077129149980183 7 1.5731347116989856 3.5161215368423933 8 1.2435255340096742 3.176468980222243 9 0.9396001883481016 2.700135314298211 >10 6.41025641025641 36.11866653909763 >50 0.158383630837721 3.1422987028962726 >100 0.006420958006934635 0.5180214042617171 >500 0.0021403193356448785 0.3833905115973921 >1k 0.0021403193356448785 0.9806869592553613 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 1435 0.9806869592553613 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 561 0.3833905115973921 TruSeq Adapter, Index 12 (95% over 23bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 344 0.23509150800267892 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 293 0.20023782513018876 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005467244372155324 0.0 2 0.0 0.0 0.0 0.011617894290830065 0.0 3 0.0 0.0 0.0 0.016401733116465974 0.0 4 0.0 0.0 0.0 0.0307532495933737 0.0 5 0.0 0.0 0.0 0.03280346623293195 0.0 6 0.0 0.0 0.0 0.034853682872490195 0.0 7 0.0 0.0 0.0 0.03758730505856785 0.0 8 0.0 0.0 0.0 0.043737954977242595 0.0 9 0.0 0.0 0.0 0.07790823230321338 0.0 10 0.0 0.0 0.0 0.10524445416398999 0.0 11 0.0 0.0 0.0 0.11891256509437831 0.0 12 0.0 0.0 0.0 0.12574662055957248 0.0 13 0.0 0.0 0.0 0.12916364829216953 0.0 14 0.0 0.0 0.0 0.13736451485040252 0.0 15 0.0 0.0 0.0 0.14351516476907727 0.0 16 0.0 0.0 0.0 0.15103262578079082 0.0 17 0.0 0.0 0.0 0.15991689788554325 0.0 18 0.0 0.0 0.0 0.16880116999029565 0.0 19 0.0 0.0 0.0 0.17290160326941215 0.0 20 0.0 0.0 0.0 0.1804190642811257 0.0 21 0.0 0.0 0.0 0.18588630865328104 0.0 22 0.0 0.0 0.0 0.19203695857195577 0.0 23 0.0 0.0 0.0 0.20160463622322758 0.0 24 0.0 0.0 0.0 0.20775528614190233 0.0 25 0.0 0.0 0.0 0.2152727471536159 0.0 26 0.0 0.0 0.0 0.22894085808400422 0.0 27 0.0 0.0 0.0 0.2508098355726255 0.0 28 0.0 0.0 0.0 0.27814605743340215 0.0 29 0.0 0.0 0.0 0.3095827125732952 0.0 30 0.0 0.0 0.0 0.343752989899266 0.0 31 0.0 0.0 0.0 0.38612413378346977 0.0 32 0.0 0.0 0.0 0.42917868321419295 0.0 33 0.0 0.0 0.0 0.48658474912182387 0.0 34 0.0 0.0 0.0 0.5583423315063625 0.0 35 0.0 0.0 0.0 0.6492352691934448 0.0 36 0.0 0.0 0.0 0.7879665951368862 0.0 37 0.0 0.0 0.0 1.0715798969424435 0.0 38 0.0 0.0 0.0 1.455653814086355 0.0 39 0.0 0.0 0.0 1.9319874800103878 0.0 40 0.0 0.0 0.0 2.5436354441452647 0.0 41 0.0 0.0 0.0 3.179202602408321 0.0 42 0.0 0.0 0.0 3.8243374383226496 0.0 43 0.0 0.0 0.0 4.5009089293768705 0.0 44 0.0 0.0 0.0 5.155611442942471 0.0 45 0.0 0.0 0.0 5.8198816341593425 0.0 46 0.0 0.0 0.0 6.562743463225948 0.0 47 0.0 0.0 0.0 7.289203559176086 0.0 48 0.0 0.0 0.0 8.012930032940147 0.0 49 0.0 0.0 0.0 8.679933846343097 0.0 50 0.0 0.0 0.0 9.455599141642633 0.0 51 0.0 0.0 0.0 10.11508549403387 0.0 52 0.0 0.0 0.0 10.824460451321023 0.0 53 0.0 0.0 0.0 11.466861665049274 0.0 54 0.0 0.0 0.0 12.144799967196533 0.0 55 0.0 0.0 0.0 12.70929294862157 0.0 56 0.0 0.0 0.0 13.294288096442191 0.0 57 0.0 0.0 0.0 13.834178478192529 0.0 58 0.0 0.0 0.0 14.353566693547284 0.0 59 0.0 0.0 0.0 14.939928652460944 0.0 60 0.0 0.0 0.0 15.49280373959515 0.0 61 0.0 0.0 0.0 16.135204953323402 0.0 62 0.0 0.0 0.0 16.676462146166777 0.0 63 0.0 0.0 0.0 17.24983939969657 0.0 64 0.0 0.0 0.0 17.829367303145034 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACGA 25 2.384122E-4 56.000004 5 CGTGATA 20 0.0069399774 52.5 24 AGGGTAC 20 0.0069399774 52.5 56 GAATAGC 40 7.439121E-7 52.5 21 AGCGGTC 20 0.0069399774 52.5 56 CTACGTG 20 0.0069399774 52.5 68 CGCTATT 20 0.0069399774 52.5 32 GAGCGGT 20 0.0069399774 52.5 55 TGTTCCG 20 0.0069399774 52.5 5 GGTATGC 20 0.0069399774 52.5 30 GTTCCGT 20 0.0069399774 52.5 6 TAGCTCG 20 0.0069399774 52.5 9 CGCCATT 50 6.151822E-8 49.000004 17 CGAGTGA 45 1.6756494E-6 46.666664 64 GAGTCCC 30 5.862503E-4 46.666664 42 GTCTCGA 45 1.6756494E-6 46.666664 60 GTCAGTA 30 5.862503E-4 46.666664 15 ACGATGT 30 5.862503E-4 46.666664 8 AGGTTAC 30 5.862503E-4 46.666664 51 ACGTCTC 45 1.6756494E-6 46.666664 58 >>END_MODULE