FastQCFastQC Report
Wed 25 May 2016
SRR1780668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79440
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA150.00221829269.991812
TGCATAT150.00221829269.9918135
CGCTACT150.00221829269.9918112
TATTCCC150.00221829269.9918130
GACGCTA150.00221829269.9918110
ACAAGTT150.00221829269.991816
ACGGAAC200.0067096452.9271471
CAGCGCA200.00693154952.49386226
CAATTAC200.00693154952.49386248
AGAGTCC200.00693154952.49386243
CACAGTG200.00693154952.4938627
CAGTGGT200.00693154952.49386269
AGTAAGA200.00693154952.4938622
TACACTG350.001249441139.9953235
GAAGAGC400.002407320534.9959131
ATCCGTC400.002407320534.9959164
AGACGCT400.002407320534.995919
GTTCTGG450.004287033331.10747160
AGAAGAG450.004287033331.10747130
ACGAATG1401.0913936E-1129.99649270
Produced by FastQC (version 0.11.5)