##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780668_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 79440 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.773363544813694 34.0 31.0 34.0 12.0 34.0 2 31.337084592145015 34.0 32.0 34.0 23.0 34.0 3 31.386165659617323 34.0 33.0 34.0 23.0 34.0 4 31.80088116817724 34.0 34.0 34.0 23.0 34.0 5 31.75723816717019 34.0 34.0 34.0 23.0 34.0 6 34.305123363544816 38.0 35.0 38.0 24.0 38.0 7 33.91183282980866 37.0 34.0 38.0 23.0 38.0 8 32.63270392749245 37.0 32.0 38.0 11.0 38.0 9 33.53383685800604 37.0 34.0 38.0 21.0 38.0 10-11 33.9418995468278 37.0 34.0 38.0 22.5 38.0 12-13 34.33601460221551 38.0 35.0 38.0 24.5 38.0 14-15 33.96920946626385 37.5 34.0 38.0 22.5 38.0 16-17 33.8419058408862 37.0 34.0 38.0 22.0 38.0 18-19 33.811379657603226 37.0 34.0 38.0 22.0 38.0 20-21 33.84574521651561 37.0 34.0 38.0 22.0 38.0 22-23 33.893063947633436 37.0 34.0 38.0 22.0 38.0 24-25 34.30930891238671 38.0 35.0 38.0 23.5 38.0 26-27 34.367547834843904 38.0 35.5 38.0 23.5 38.0 28-29 34.487009063444106 38.0 35.5 38.0 24.5 38.0 30-31 34.30574647532729 38.0 35.0 38.0 24.0 38.0 32-33 34.07108509566969 38.0 34.0 38.0 23.0 38.0 34-35 33.95443101711984 37.0 34.0 38.0 23.0 38.0 36-37 33.9175415407855 37.0 34.0 38.0 22.5 38.0 38-39 34.44809919436052 38.0 35.0 38.0 24.5 38.0 40-41 33.92637839879154 37.5 34.0 38.0 22.5 38.0 42-43 34.26625755287009 38.0 34.5 38.0 24.0 38.0 44-45 33.30110775427996 37.0 34.0 38.0 21.0 38.0 46-47 34.05606117824773 37.0 34.0 38.0 23.0 38.0 48-49 33.77516993957704 37.0 34.0 38.0 22.5 38.0 50-51 33.778008559919435 37.0 34.0 38.0 22.5 38.0 52-53 33.78346550855992 37.0 34.0 38.0 22.5 38.0 54-55 33.49143378650554 37.0 34.0 38.0 21.0 38.0 56-57 33.588003524672715 37.0 34.0 38.0 22.0 38.0 58-59 33.44970417925478 37.0 33.5 38.0 22.0 38.0 60-61 33.463997985901315 37.0 33.5 38.0 21.5 38.0 62-63 33.52994083585095 37.0 34.0 38.0 21.5 38.0 64-65 33.60336732124874 37.0 34.0 38.0 22.0 38.0 66-67 33.547098439073515 37.0 33.5 38.0 22.0 38.0 68-69 33.114639979859014 37.0 32.5 38.0 20.5 38.0 70-71 32.950673464249746 37.0 32.0 38.0 20.0 38.0 72-73 33.05188192346425 37.0 32.0 38.0 20.0 38.0 74-75 32.680721299093655 37.0 31.5 38.0 19.5 38.0 76 31.884101208459214 36.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1592064159482085 1101 2 -0.18038259640458776 1101 3 0.14000554033570722 1101 4 -1.7119586814656422 1101 5 -0.837041829613419 1101 6 -2.276979911856131 1101 7 -0.5596266268601084 1101 8 -0.47746510768496364 1101 9 -1.1301637869641041 1101 10-11 -0.6862272277522194 1101 12-13 -1.1711293267103784 1101 14-15 -0.25303267050372114 1101 16-17 -1.122767799723924 1101 18-19 0.38390417967234214 1101 20-21 -1.1972484385654454 1101 22-23 -0.09290794138465941 1101 24-25 -1.3642467956212485 1101 26-27 -0.7920630694076038 1101 28-29 -0.2815513076487477 1101 30-31 -1.1396717097768558 1101 32-33 -0.33249945318772944 1101 34-35 -1.4273386331837514 1101 36-37 -0.3117360791397914 1101 38-39 -0.465462517049005 1101 40-41 -0.2153688848064661 1101 42-43 -0.46597276675737476 1101 44-45 0.4626618350563234 1101 46-47 0.6494810208369088 1101 48-49 -1.428978250944425 1101 50-51 -0.8547004923957644 1101 52-53 0.7799177668312396 1101 54-55 -1.4466684393950402 1101 56-57 -0.5733671516591698 1101 58-59 -0.26106463567155913 1101 60-61 -0.024688841491361302 1101 62-63 -1.1507496923793639 1101 64-65 -0.07199266011515704 1101 66-67 -0.701032782117224 1101 68-69 0.38561646776857117 1101 70-71 0.5832271557397988 1101 72-73 -0.993556883507928 1101 74-75 -1.28427049422924 1101 76 -1.591919496701255 1102 1 0.9694167724575884 1102 2 -0.07108307949637549 1102 3 0.7166963615917474 1102 4 0.4100219948628627 1102 5 0.7408567211112143 1102 6 0.825073228240484 1102 7 1.379986899709941 1102 8 1.7592498681604454 1102 9 0.4314304159344502 1102 10-11 -0.1795847156749275 1102 12-13 -4.212494061009892 1102 14-15 -0.9121510279916478 1102 16-17 -2.668661519530687 1102 18-19 -0.6103591053518116 1102 20-21 0.09411629573406799 1102 22-23 -0.8957461056358653 1102 24-25 0.3184222865043509 1102 26-27 -5.853657272306151 1102 28-29 0.18704772616767684 1102 30-31 0.41769543998160685 1102 32-33 -6.826158873477581 1102 34-35 -0.2555391162755427 1102 36-37 0.6031189933239816 1102 38-39 0.7483055988930261 1102 40-41 0.7272639654350783 1102 42-43 0.49960694338754763 1102 44-45 -5.4425314016586555 1102 46-47 0.2165100063441514 1102 48-49 1.1129903480893972 1102 50-51 -6.127043487564851 1102 52-53 -2.045263392589053 1102 54-55 0.8558677924890219 1102 56-57 -1.0791038666350161 1102 58-59 0.6106141082897949 1102 60-61 0.5945744435327995 1102 62-63 -4.540242446002548 1102 64-65 -0.6930675393422092 1102 66-67 0.46472808744799465 1102 68-69 -6.274709619187952 1102 70-71 -0.5218453080283112 1102 72-73 0.7319865947529323 1102 74-75 -6.8811907840843105 1102 76 -2.2024267430780675 1103 1 -0.10692533817723415 1103 2 -0.5900325249614333 1103 3 -0.278676843824325 1103 4 0.14030708705854522 1103 5 0.33567198953334554 1103 6 -1.035782728757539 1103 7 0.5519267513803499 1103 8 1.3086136839051576 1103 9 0.2017917141585741 1103 10-11 0.9253006350769297 1103 12-13 -1.675887950919396 1103 14-15 -0.14437529144677086 1103 16-17 -0.7046212392013658 1103 18-19 -1.0254778370657647 1103 20-21 -0.7649025516497474 1103 22-23 -0.5439055939668194 1103 24-25 0.2801831900901206 1103 26-27 -0.6985235715508935 1103 28-29 0.2569259326043678 1103 30-31 0.1169878125746493 1103 32-33 -4.723076611791527 1103 34-35 -0.7187629034429861 1103 36-37 0.02351091004167216 1103 38-39 0.7531024962190145 1103 40-41 1.0697148057426276 1103 42-43 0.4542915729037702 1103 44-45 -5.092732122894283 1103 46-47 -0.22010971196570495 1103 48-49 -0.23711511561068477 1103 50-51 0.152469338181902 1103 52-53 -0.31409376613467543 1103 54-55 0.14898883760027104 1103 56-57 1.0111526483000048 1103 58-59 0.8457301183598815 1103 60-61 0.8754821117644056 1103 62-63 0.25645075669187634 1103 64-65 0.8993662906911268 1103 66-67 0.2359058788339894 1103 68-69 -2.8079817155341473 1103 70-71 0.12277604326786928 1103 72-73 0.7791390748509102 1103 74-75 -1.4499803329718368 1103 76 0.26828156473985487 1104 1 -2.2191970546001727 1104 2 -0.1644423010020475 1104 3 -0.04568719941881483 1104 4 -0.6209975830674743 1104 5 -0.5658487778139616 1104 6 -0.45461149080350083 1104 7 0.6106458923826565 1104 8 1.448268197911041 1104 9 0.426784413576776 1104 10-11 -0.44618666191073686 1104 12-13 -1.3220680618882383 1104 14-15 -0.18561796278581255 1104 16-17 -1.3714676124969643 1104 18-19 0.3347441006209664 1104 20-21 1.0726314241347836 1104 22-23 0.281194929428743 1104 24-25 0.5175531835757496 1104 26-27 0.943062788715963 1104 28-29 -0.5324354349630838 1104 30-31 -0.6445864174947573 1104 32-33 -0.700231406589019 1104 34-35 -0.09312550649350726 1104 36-37 0.09441640681818342 1104 38-39 -0.4334779112657685 1104 40-41 0.842801491727748 1104 42-43 0.89170873937951 1104 44-45 -3.0063156977083985 1104 46-47 0.5865259553074793 1104 48-49 0.8808173596234994 1104 50-51 1.2510047291561364 1104 52-53 -0.0520590378286343 1104 54-55 -0.44487418102183085 1104 56-57 0.7936627006395582 1104 58-59 0.7606614928909536 1104 60-61 0.22509012668005823 1104 62-63 0.7463534904789739 1104 64-65 0.2809171588987809 1104 66-67 0.5369144822888501 1104 68-69 -3.134497428632514 1104 70-71 -0.13380009618447986 1104 72-73 0.970492939666606 1104 74-75 -0.5847827679966571 1104 76 0.27964800901957076 1105 1 -1.4323581110257777 1105 2 0.5363143965086472 1105 3 -0.593354927007713 1105 4 0.428387995416184 1105 5 0.18572679708644557 1105 6 0.23238132162404668 1105 7 1.5212965679301576 1105 8 1.3101234768773793 1105 9 0.7234562092052172 1105 10-11 0.8680008894667068 1105 12-13 -0.6949154787885234 1105 14-15 0.20438688513695524 1105 16-17 1.413432161969233 1105 18-19 0.9106351699040971 1105 20-21 0.07031285237498963 1105 22-23 0.6210237766770135 1105 24-25 0.539495303593462 1105 26-27 0.5720913071486109 1105 28-29 -0.2280175018644215 1105 30-31 0.4499902323798253 1105 32-33 1.1746711080074448 1105 34-35 0.9138196698233401 1105 36-37 1.179381522852161 1105 38-39 0.8191094040389117 1105 40-41 0.7513759916539797 1105 42-43 0.9158745804941049 1105 44-45 1.7213048153282955 1105 46-47 1.0593881267553158 1105 48-49 0.9621465335796131 1105 50-51 0.5893475826856331 1105 52-53 0.037543221724931186 1105 54-55 -0.5166023600998813 1105 56-57 0.02478214924104094 1105 58-59 0.80268033655598 1105 60-61 0.4063708214084656 1105 62-63 0.39141177094184343 1105 64-65 0.4418576559162588 1105 66-67 0.9333164804761509 1105 68-69 1.1990501969392255 1105 70-71 1.608174483837864 1105 72-73 0.6988511800814194 1105 74-75 0.31701517901343834 1105 76 -1.028258320681335 1106 1 -0.7359434660834268 1106 2 -0.20271981296377106 1106 3 -0.595926433868577 1106 4 -0.14731337254115573 1106 5 -0.16871959121109015 1106 6 -0.6364296726216807 1106 7 0.7111243612821809 1106 8 0.41477537494453287 1106 9 1.2066887197968725 1106 10-11 0.4365884577064847 1106 12-13 0.08223815820745273 1106 14-15 0.3119896927165797 1106 16-17 0.6715945884599748 1106 18-19 0.3490981676066056 1106 20-21 -0.03980876725277227 1106 22-23 0.513252345696678 1106 24-25 0.44578284864752504 1106 26-27 0.29952211885951385 1106 28-29 -1.245354095250164 1106 30-31 -0.31205053232729796 1106 32-33 0.713644191497103 1106 34-35 0.4762524839371167 1106 36-37 0.8073350671052637 1106 38-39 0.32967998347107397 1106 40-41 0.1394522094311057 1106 42-43 0.6763977345948149 1106 44-45 1.4195216228657586 1106 46-47 1.1104494002835494 1106 48-49 0.8449321921593835 1106 50-51 0.6002870258068498 1106 52-53 0.17760603394170005 1106 54-55 0.1532119433800787 1106 56-57 0.994619638438607 1106 58-59 0.5381734546134425 1106 60-61 1.002602088402547 1106 62-63 1.2343917679909282 1106 64-65 0.8981420392815593 1106 66-67 1.1565317071692292 1106 68-69 0.9970815187359108 1106 70-71 0.02648698515523762 1106 72-73 -0.03668409382621718 1106 74-75 -0.9723425766091012 1106 76 -0.07441897662636521 1107 1 0.7218269110006545 1107 2 0.9151690220593061 1107 3 1.0101693020671796 1107 4 0.5737624447553848 1107 5 0.17691622439922128 1107 6 0.5143196476152312 1107 7 0.5521335723354426 1107 8 0.30571819053376714 1107 9 -0.5452662577551379 1107 10-11 -0.14962025590774175 1107 12-13 0.1861417899339486 1107 14-15 -0.6612518813828672 1107 16-17 0.9773088139516162 1107 18-19 1.1286968439129126 1107 20-21 1.0064362003926135 1107 22-23 -0.5265533158340006 1107 24-25 0.05711644896791057 1107 26-27 0.08530716617944734 1107 28-29 -0.8579734490009798 1107 30-31 0.6966862676221126 1107 32-33 1.2517636190206574 1107 34-35 0.8169033702638657 1107 36-37 0.3952405536891703 1107 38-39 1.0702107256248183 1107 40-41 0.6015962559711667 1107 42-43 0.2947292060447637 1107 44-45 0.09795679770715537 1107 46-47 0.5704013426143462 1107 48-49 0.9489306745487767 1107 50-51 1.0937528690292453 1107 52-53 1.2115520322976252 1107 54-55 0.38207605399703226 1107 56-57 0.7450464664216554 1107 58-59 1.2982750502123679 1107 60-61 0.42839725312653343 1107 62-63 -0.4566058502035304 1107 64-65 0.14009554137881253 1107 66-67 1.0038010619739453 1107 68-69 1.8510325757938517 1107 70-71 0.8174255851594481 1107 72-73 0.8750626338259124 1107 74-75 0.4469711261500038 1107 76 -0.07231118411745996 1108 1 -0.38804958365006925 1108 2 0.11484255887491557 1108 3 -0.06399290686925951 1108 4 -0.5725953274283739 1108 5 0.4267392262872036 1108 6 0.03998437385815379 1108 7 0.5061730625808849 1108 8 0.6902367556690763 1108 9 -0.00496968758521632 1108 10-11 -0.3051130973174381 1108 12-13 -0.13172715279733183 1108 14-15 0.7337685717322984 1108 16-17 -1.0583268059351028 1108 18-19 -0.04796693834008181 1108 20-21 0.3712686421798992 1108 22-23 -0.7179079413846594 1108 24-25 -1.1112382552485798 1108 26-27 -0.8598037526374185 1108 28-29 0.08811095943199376 1108 30-31 0.6279913337014094 1108 32-33 0.6569221306632045 1108 34-35 -0.09804903691045297 1108 36-37 -0.46924772510252666 1108 38-39 0.6715716444416842 1108 40-41 0.5085247487339046 1108 42-43 0.48540533883268466 1108 44-45 0.38977751828613805 1108 46-47 0.550587387296531 1108 48-49 0.27055591054626404 1108 50-51 -0.12546912593614223 1108 52-53 -0.3580868915538531 1108 54-55 0.2319029891763904 1108 56-57 0.6026809849867902 1108 58-59 -0.863452058031811 1108 60-61 0.40533833242168527 1108 62-63 0.2081400591734308 1108 64-65 -0.10363085887291845 1108 66-67 0.0751706340318492 1108 68-69 1.3886250081412328 1108 70-71 1.8815579010814147 1108 72-73 1.0755424953740587 1108 74-75 0.9930004685036806 1108 76 1.1029562796962615 1109 1 -0.013049583650069252 1109 2 -0.9967645839822268 1109 3 0.029757093130740486 1109 4 -0.13509532742837393 1109 5 0.11423922628720007 1109 6 0.12034151671529969 1109 7 -0.9223983659905457 1109 8 0.5563081842404998 1109 9 0.9905660267004919 1109 10-11 1.0609583312539925 1109 12-13 -0.20092358136876243 1109 14-15 -0.37114214255341693 1109 16-17 -0.39761252022082516 1109 18-19 0.1640866330884947 1109 20-21 0.5699293564656074 1109 22-23 0.9740563443296253 1109 24-25 0.6342974590371355 1109 26-27 0.4616248187911509 1109 28-29 -0.08153189771086033 1109 30-31 0.18379490512997876 1109 32-33 0.6368328449489198 1109 34-35 -0.18287046548188357 1109 36-37 0.00843084632604274 1109 38-39 0.6537145015845383 1109 40-41 0.27191760587675873 1109 42-43 0.22870891026126117 1109 44-45 0.11522394685756865 1109 46-47 0.04165881586796161 1109 48-49 0.15225233911768754 1109 50-51 0.7964058740638578 1109 52-53 0.7914666798747163 1109 54-55 -0.4265791536807555 1109 56-57 -0.07142615787034856 1109 58-59 -0.3545234866032345 1109 60-61 -0.23528666757831473 1109 62-63 0.18581863060200732 1109 64-65 -0.21077371601577255 1109 66-67 -0.2083115088253038 1109 68-69 1.136392865284094 1109 70-71 0.47307575822427594 1109 72-73 -0.5405289331973719 1109 74-75 1.2407683256465418 1109 76 -0.03543657744659612 1110 1 0.4745828497060458 1110 2 -0.15965761200683204 1110 3 -0.07918002238532296 1110 4 0.3407249049708021 1110 5 -0.269198060110611 1110 6 0.15782870058406218 1110 7 0.007775078985531536 1110 8 1.9506605424086487 1110 9 -1.2143639117820726 1110 10-11 -2.1423589107145666 1110 12-13 -0.661780980548528 1110 14-15 -0.8482973886231306 1110 16-17 -0.6848647416835618 1110 18-19 -0.22267216732161899 1110 20-21 -0.9584690543341168 1110 22-23 -0.4628242093272448 1110 24-25 -0.2001501657065461 1110 26-27 -2.030621421810345 1110 28-29 -0.2621005067334181 1110 30-31 -0.9292754127100764 1110 32-33 0.5557494546550075 1110 34-35 0.23462568967874375 1110 36-37 -0.5108467510765493 1110 38-39 -0.08419561461505509 1110 40-41 -0.035188938894258115 1110 42-43 0.11419677765701408 1110 44-45 0.6180862161671996 1110 46-47 -0.652708494453293 1110 48-49 0.2612001347431203 1110 50-51 -0.18679431390606283 1110 52-53 -0.07598248280470443 1110 54-55 -0.4998981044668085 1110 56-57 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0.8386581514426155 2116 50-51 0.3128186891899105 2116 52-53 1.0460622681100133 2116 54-55 0.30578254099711444 2116 56-57 -0.18627209624570185 2116 58-59 -0.08688518128110445 2116 60-61 -1.0631052950292954 2116 62-63 0.7938368378849177 2116 64-65 0.9829412699786175 2116 66-67 -0.09718580854519132 2116 68-69 0.35295448993395695 2116 70-71 -2.262787337013819 2116 72-73 0.5358015990155067 2116 74-75 -1.5585138872386146 2116 76 -0.44326220769869806 2117 1 -0.13223758904090488 2117 2 -0.4922160664620101 2117 3 -0.09389519797437984 2117 4 -0.5060522007437385 2117 5 -0.6306226335510736 2117 6 -1.2557534967617912 2117 7 -2.237846479198094 2117 8 -2.865693163468393 2117 9 -0.29935984931028514 2117 10-11 -0.32250696524196343 2117 12-13 0.5233436019196489 2117 14-15 -0.008313638510287547 2117 16-17 -0.12133219677068752 2117 18-19 -0.19848917553684942 2117 20-21 -0.14633580525945433 2117 22-23 -0.4879551111959799 2117 24-25 -0.19079519594938432 2117 26-27 0.31632495356204515 2117 28-29 0.07652908611662923 2117 30-31 -0.3572681002608604 2117 32-33 0.9181670767548482 2117 34-35 -0.16775085631746833 2117 36-37 -2.0279994097386407 2117 38-39 -0.04995800515401072 2117 40-41 -1.6306683105652908 2117 42-43 -1.8559440546983126 2117 44-45 -0.4193110935737039 2117 46-47 -0.6003138930269074 2117 48-49 -0.4179548711248984 2117 50-51 0.40729704657059074 2117 52-53 0.5794973402520753 2117 54-55 -1.6518571186403221 2117 56-57 -1.0023560770078177 2117 58-59 -1.0561323140965015 2117 60-61 -0.0920083656915196 2117 62-63 -0.7749293478346502 2117 64-65 -0.8762892146680699 2117 66-67 -2.5436804441352727 2117 68-69 -0.7734791023708922 2117 70-71 0.755294423992467 2117 72-73 0.8375034118161082 2117 74-75 -0.7391002727362093 2117 76 -1.2878792998185666 2118 1 -0.4088895900562193 2118 2 -0.26882576271381 2118 3 -0.14296872365337165 2118 4 -0.48174811410358487 2118 5 -0.33901589863272363 2118 6 -0.06565704190097676 2118 7 -0.06547572025063886 2118 8 -3.6665857939785873 2118 9 -1.8321037362719572 2118 10-11 -1.308546393281567 2118 12-13 -0.6125287223040559 2118 14-15 -2.0701632027712265 2118 16-17 -1.070418413878734 2118 18-19 -1.3111996417353353 2118 20-21 -2.4997074949117106 2118 22-23 -1.1542868651514766 2118 24-25 -0.9531164583235281 2118 26-27 -0.0997741241941128 2118 28-29 -0.909256513341866 2118 30-31 -1.0537631506035225 2118 32-33 -0.8057520365373065 2118 34-35 0.341122567830098 2118 36-37 -0.5989021933920853 2118 38-39 -0.5586093292931054 2118 40-41 -1.612741667025226 2118 42-43 -0.8318091871122206 2118 44-45 -0.34005311912578406 2118 46-47 -1.0630116677963528 2118 48-49 -0.9865308927849412 2118 50-51 -0.41136358461648115 2118 52-53 -2.028950921022144 2118 54-55 0.13460958751078778 2118 56-57 0.6889037588497047 2118 58-59 0.3656779067343692 2118 60-61 -1.8640702998653111 2118 62-63 -0.7277383841321381 2118 64-65 -0.35673383773262657 2118 66-67 -3.0052465664806505 2118 68-69 -0.07769265681712056 2118 70-71 -1.4150668586879611 2118 72-73 -2.2443245770154405 2118 74-75 -0.44130646615359126 2118 76 -0.7448976600859325 2119 1 -0.3053214565140472 2119 2 0.5819691822515409 2119 3 0.3370879201984103 2119 4 0.37421772793731023 2119 5 0.45807466716211565 2119 6 0.5262497532156374 2119 7 -0.8056220502010731 2119 8 1.2377418821215684 2119 9 0.6612341743423258 2119 10-11 0.338502333304568 2119 12-13 0.9315204548581733 2119 14-15 0.3598365826687342 2119 16-17 0.15006348798150526 2119 18-19 -0.05042336349387 2119 20-21 -0.5541628342384186 2119 22-23 0.7620561734478812 2119 24-25 0.2759263893173838 2119 26-27 0.6918666164671663 2119 28-29 0.804885139199591 2119 30-31 0.46546142939519086 2119 32-33 0.739959980970788 2119 34-35 0.8853433930280232 2119 36-37 0.2044642537081387 2119 38-39 -0.45979370073945347 2119 40-41 -0.20983008721961482 2119 42-43 -0.677669251051114 2119 44-45 -0.7072726355074366 2119 46-47 0.1582962902357039 2119 48-49 -0.5665510614291378 2119 50-51 0.839521475567615 2119 52-53 -0.7434465976372451 2119 54-55 -0.1625775303041337 2119 56-57 1.1261985858070247 2119 58-59 1.877886373273725 2119 60-61 0.9069083084982381 2119 62-63 0.19132956703399628 2119 64-65 0.857472182822228 2119 66-67 0.5704073052553582 2119 68-69 -0.36416250040284837 2119 70-71 -0.9495895698686851 2119 72-73 -1.7639568210989438 2119 74-75 0.3793320645392271 2119 76 1.389695855909519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 12.0 13 78.0 14 256.0 15 492.0 16 653.0 17 713.0 18 756.0 19 701.0 20 681.0 21 666.0 22 643.0 23 640.0 24 739.0 25 846.0 26 982.0 27 1207.0 28 1449.0 29 1758.0 30 2223.0 31 2865.0 32 3577.0 33 4700.0 34 6737.0 35 9905.0 36 16461.0 37 19699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.12279588988964 22.878345807433718 11.921857160979323 31.077001141697323 2 16.839123867069485 21.74093655589124 36.744712990936556 24.67522658610272 3 17.32124874118832 25.18378650553877 28.349697885196374 29.145266868076536 4 12.818479355488419 18.42019133937563 38.58635448136959 30.174974823766366 5 12.83987915407855 35.541289023162136 36.14929506545821 15.469536757301109 6 26.681772406847937 36.92598187311179 19.856495468277945 16.535750251762334 7 26.852970795568982 29.85775427995972 22.964501510574017 20.324773413897283 8 22.47860020140987 34.79229607250755 21.058660624370596 21.670443101711985 9 23.094159113796575 17.288519637462237 23.022406847935546 36.59491440080564 10-11 24.283106747230615 25.764728096676738 26.95682275931521 22.995342396777442 12-13 22.852467270896273 24.03637965760322 26.516238670694865 26.594914400805642 14-15 21.38406344410876 25.757175226586103 24.13645518630413 28.72230614300101 16-17 21.613796576032225 27.83358509566969 26.11404833836858 24.43856998992951 18-19 21.250629405840886 27.100327291037264 26.662260825780464 24.98678247734139 20-21 20.75780463242699 27.29607250755287 26.8202416918429 25.125881168177237 22-23 21.983257804632427 27.275302114803623 26.718277945619334 24.023162134944613 24-25 21.518126888217523 27.804003021148038 26.632049345417926 24.045820745216513 26-27 20.76409869083585 28.271651560926486 27.070745216515608 23.893504531722055 28-29 21.718277945619334 26.782477341389725 28.307527693856997 23.191717019133936 30-31 21.51183282980866 27.51573514602216 27.82666163141994 23.145770392749245 32-33 20.32351460221551 28.513972809667678 27.256419939577036 23.90609264853978 34-35 21.57477341389728 27.286631419939578 27.711480362537767 23.42711480362538 36-37 21.98577542799597 27.22872608257805 27.114803625377643 23.67069486404834 38-39 21.219159113796575 27.67623363544814 27.865684793554884 23.238922457200403 40-41 21.25818227593152 26.284617321248742 28.284869083585097 24.17233131923464 42-43 20.829556898288015 26.105236656596176 28.58383685800604 24.481369587109768 44-45 20.416666666666668 25.857880161127895 28.782729103726084 24.942724068479354 46-47 20.725075528700906 25.577165156092647 29.473187311178243 24.224572004028197 48-49 19.502139979859013 26.116565961732125 29.96475327291037 24.41654078549849 50-51 19.653197381671703 26.158736153071498 29.215130916414907 24.972935548841892 52-53 18.653071500503522 26.697507552870093 29.6714501510574 24.977970795568982 54-55 18.52467270896274 27.015357502517624 29.121978851963746 25.337990936555894 56-57 18.47809667673716 27.09025679758308 28.82930513595166 25.602341389728096 58-59 18.834340382678754 26.599320241691842 28.48942598187311 26.076913393756296 60-61 18.553625377643503 26.130412890231618 29.01686807653575 26.29909365558912 62-63 19.436052366565963 26.28965256797583 28.46110271903323 25.813192346424973 64-65 19.732502517623363 26.311052366565963 27.43076535750252 26.525679758308158 66-67 19.78159617321249 26.602467270896273 26.548338368580062 27.067598187311177 68-69 19.583333333333332 26.086983887210476 26.747860020140983 27.581822759315205 70-71 20.314073514602214 26.411127895266866 26.247482376636455 27.027316213494462 72-73 20.526812688821753 26.495468277945623 25.553877139979857 27.42384189325277 74-75 20.49911883182276 27.44838872104733 25.213368580060425 26.839123867069485 76 21.261329305135952 27.045568982880162 25.16364551863041 26.529456193353475 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 2.5 11 2.5 12 0.0 13 1.5 14 2.5 15 4.0 16 4.5 17 3.0 18 8.5 19 19.0 20 30.0 21 36.0 22 40.5 23 69.5 24 104.0 25 114.0 26 186.0 27 307.0 28 378.0 29 400.0 30 517.0 31 742.5 32 1034.0 33 1217.0 34 1420.5 35 1864.5 36 2235.0 37 2365.0 38 2739.5 39 3465.5 40 4184.5 41 4749.5 42 4947.0 43 5161.5 44 5558.5 45 5813.0 46 5885.0 47 5823.5 48 5522.0 49 5149.5 50 5017.0 51 4680.0 52 3853.5 53 3122.5 54 2881.0 55 2646.0 56 2104.0 57 1602.5 58 1408.0 59 1217.5 60 843.0 61 598.0 62 537.0 63 454.5 64 325.0 65 231.5 66 166.5 67 148.0 68 132.0 69 93.5 70 54.5 71 38.0 72 39.5 73 36.0 74 21.0 75 11.0 76 10.0 77 10.0 78 13.5 79 16.0 80 11.5 81 6.5 82 5.0 83 4.0 84 2.5 85 1.5 86 2.0 87 2.0 88 2.0 89 1.5 90 0.5 91 1.0 92 2.0 93 2.5 94 2.0 95 0.5 96 0.0 97 0.5 98 1.0 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7678751258811681 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 79440.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.80387713997986 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74182825484765 41.88821752265861 2 11.086980609418283 12.595669687814704 3 5.82825484764543 9.93202416918429 4 3.011634349030471 6.842900302114803 5 1.8681440443213297 5.305891238670695 6 1.1567867036011081 3.9425981873111784 7 0.853185595567867 3.3924974823766365 8 0.6227146814404432 2.8298086606243706 9 0.4321329639889197 2.209214501510574 >10 1.3983379501385043 11.061178247734139 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0025176233635448137 0.0 2 0.0 0.0 0.0 0.0025176233635448137 0.0 3 0.0 0.0 0.0 0.0037764350453172208 0.0 4 0.0 0.0 0.0 0.0075528700906344415 0.0 5 0.0 0.0 0.0 0.008811681772406849 0.0 6 0.0 0.0 0.0 0.008811681772406849 0.0 7 0.0 0.0 0.0 0.008811681772406849 0.0 8 0.0 0.0 0.0 0.013846928499496475 0.0 9 0.0 0.0 0.0 0.025176233635448138 0.0 10 0.0 0.0 0.0 0.03272910372608258 0.0 11 0.0 0.0 0.0 0.04405840886203424 0.0 12 0.0 0.0 0.0 0.04783484390735146 0.0 13 0.0 0.0 0.0 0.04909365558912387 0.0 14 0.0 0.0 0.0 0.06042296072507553 0.0 15 0.0 0.0 0.0 0.06294058408862034 0.0 16 0.0 0.0 0.0 0.07678751258811682 0.0 17 0.0 0.0 0.0 0.08434038267875126 0.0 18 0.0 0.0 0.0 0.08685800604229607 0.0 19 0.0 0.0 0.0 0.09441087613293052 0.0 20 0.0 0.0 0.0 0.09692849949647532 0.0 21 0.0 0.0 0.0 0.10070493454179255 0.0 22 0.0 0.0 0.0 0.10322255790533737 0.0 23 0.0 0.0 0.0 0.11203423967774422 0.0 24 0.0 0.0 0.0 0.11958710976837865 0.0 25 0.0 0.0 0.0 0.12210473313192347 0.0 26 0.0 0.0 0.0 0.1309164149043303 0.0 27 0.0 0.0 0.0 0.15609264853977844 0.0 28 0.0 0.0 0.0 0.18756294058408862 0.0 29 0.0 0.0 0.0 0.21651560926485397 0.0 30 0.0 0.0 0.0 0.2542799597180262 0.0 31 0.0 0.0 0.0 0.29204431017119836 0.0 32 0.0 0.0 0.0 0.3323262839879154 0.0 33 0.0 0.0 0.0 0.3637965760322256 0.0 34 0.0 0.0 0.0 0.4229607250755287 0.0 35 0.0 0.0 0.0 0.5412890231621349 0.0 36 0.0 0.0 0.0 0.7099697885196374 0.0 37 0.0 0.0 0.0 1.0876132930513596 0.0 38 0.0 0.0 0.0 1.4803625377643506 0.0 39 0.0 0.0 0.0 1.948640483383686 0.0 40 0.0 0.0 0.0 2.6221047331319234 0.0 41 0.0 0.0 0.0 3.442849949647533 0.0 42 0.0 0.0 0.0 4.4159113796576035 0.0 43 0.0 0.0 0.0 5.44939577039275 0.0 44 0.0 0.0 0.0 6.4287512588116815 0.0 45 0.0 0.0 0.0 7.474823766364552 0.0 46 0.0 0.0 0.0 8.508308157099698 0.0 47 0.0 0.0 0.0 9.5027693856999 0.0 48 0.0 0.0 0.0 10.318479355488419 0.0 49 0.0 0.0 0.0 11.085095669687815 0.0 50 0.0 0.0 0.0 11.797583081570997 0.0 51 0.0 0.0 0.0 12.653575025176234 0.0 52 0.0 0.0 0.0 13.653071500503525 0.0 53 0.0 0.0 0.0 14.592145015105741 0.0 54 0.0 0.0 0.0 15.553877139979859 0.0 55 0.0 0.0 0.0 16.435045317220546 0.0 56 0.0 0.0 0.0 17.246978851963746 0.0 57 0.0 0.0 0.0 17.979607250755286 0.0 58 0.0 0.0 0.0 18.716012084592144 0.0 59 0.0 0.0 0.0 19.28877139979859 0.0 60 0.0 0.0 0.0 19.955941591137965 0.0 61 0.0 0.0 0.0 20.66339375629406 0.0 62 0.0 0.0 0.0 21.307905337361532 0.0 63 0.0 0.0 0.0 22.006545820745217 0.0 64 0.0 0.0 0.0 22.7051863041289 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAACA 15 0.002218292 69.99181 2 TGCATAT 15 0.002218292 69.99181 35 CGCTACT 15 0.002218292 69.99181 12 TATTCCC 15 0.002218292 69.99181 30 GACGCTA 15 0.002218292 69.99181 10 ACAAGTT 15 0.002218292 69.99181 6 ACGGAAC 20 0.00670964 52.927147 1 CAGCGCA 20 0.006931549 52.493862 26 CAATTAC 20 0.006931549 52.493862 48 AGAGTCC 20 0.006931549 52.493862 43 CACAGTG 20 0.006931549 52.493862 7 CAGTGGT 20 0.006931549 52.493862 69 AGTAAGA 20 0.006931549 52.493862 2 TACACTG 35 0.0012494411 39.995323 5 GAAGAGC 40 0.0024073205 34.99591 31 ATCCGTC 40 0.0024073205 34.99591 64 AGACGCT 40 0.0024073205 34.99591 9 GTTCTGG 45 0.0042870333 31.107471 60 AGAAGAG 45 0.0042870333 31.107471 30 ACGAATG 140 1.0913936E-11 29.996492 70 >>END_MODULE