FastQCFastQC Report
Wed 25 May 2016
SRR1780668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79440
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT10841.3645518630412892TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT4740.5966767371601208TruSeq Adapter, Index 5 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1510.19008056394763342RNA PCR Primer, Index 5 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1370.17245720040281975No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG810.10196374622356497TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG308.308765E-658.3333325
CGTGCGA252.3774595E-455.99999610
GTTTAGT252.3774595E-455.99999653
CCGTGCG252.3774595E-455.9999969
CCCGTGC252.3774595E-455.9999968
TATAGTC252.3774595E-455.9999965
AATTTAG200.00692834452.50000416
ATGGCTC200.00692834452.5000047
ACCTATG200.00692834452.5000042
ATGGTTC200.00692834452.50000416
TTTTCCG200.00692834452.50000426
TCTATCC200.00692834452.50000417
AGCCCGT404.5560824E-543.7500046
GCGAGAA350.00124872340.013
AGTCATA350.00124872340.045
GTGCGAG350.00124872340.011
CCGTCTT3250.038.76923447
CGTATGC3350.038.65671541
CGTCTTC3200.038.28125448
TATGCCG3400.038.08823443
Produced by FastQC (version 0.11.5)