##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780668_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 79440 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.871034743202415 34.0 34.0 34.0 31.0 34.0 2 33.35867321248741 34.0 34.0 34.0 33.0 34.0 3 33.48426485397785 34.0 34.0 34.0 34.0 34.0 4 33.52356495468278 34.0 34.0 34.0 34.0 34.0 5 33.54516616314199 34.0 34.0 34.0 34.0 34.0 6 37.29203172205438 38.0 38.0 38.0 36.0 38.0 7 36.97759315206445 38.0 37.0 38.0 35.0 38.0 8 36.9742195367573 38.0 37.0 38.0 35.0 38.0 9 36.750843403826785 38.0 37.0 38.0 34.0 38.0 10-11 36.519391993957704 38.0 37.0 38.0 34.0 38.0 12-13 36.410983131923466 38.0 37.0 38.0 34.0 38.0 14-15 36.66951158106747 38.0 37.0 38.0 34.5 38.0 16-17 36.775333585095666 38.0 37.0 38.0 34.5 38.0 18-19 36.790741440080566 38.0 37.0 38.0 34.0 38.0 20-21 36.53659994964754 38.0 37.0 38.0 34.0 38.0 22-23 36.58790281973816 38.0 37.0 38.0 34.0 38.0 24-25 35.973262839879155 38.0 36.5 38.0 32.5 38.0 26-27 36.18165281973816 38.0 37.0 38.0 34.0 38.0 28-29 36.08726082578046 38.0 37.0 38.0 33.0 38.0 30-31 35.80857250755287 38.0 36.5 38.0 32.5 38.0 32-33 36.26655337361531 38.0 37.0 38.0 34.0 38.0 34-35 36.337424471299094 38.0 37.0 38.0 34.0 38.0 36-37 36.439570745216514 38.0 37.0 38.0 34.0 38.0 38-39 36.0484390735146 38.0 37.0 38.0 32.5 38.0 40-41 35.608282980866065 38.0 36.0 38.0 31.5 38.0 42-43 36.03242698892245 38.0 37.0 38.0 32.5 38.0 44-45 36.21295946626385 38.0 37.0 38.0 34.0 38.0 46-47 36.50098816717019 38.0 37.0 38.0 34.0 38.0 48-49 36.29835725075529 38.0 37.0 38.0 34.0 38.0 50-51 36.43578172205438 38.0 37.0 38.0 34.0 38.0 52-53 36.35685422960725 38.0 37.0 38.0 34.0 38.0 54-55 36.539444864048335 38.0 37.0 38.0 34.0 38.0 56-57 36.58850075528701 38.0 37.0 38.0 34.0 38.0 58-59 36.62530211480363 38.0 37.0 38.0 34.0 38.0 60-61 36.3237915407855 38.0 37.0 38.0 34.0 38.0 62-63 36.586203423967774 38.0 37.0 38.0 34.0 38.0 64-65 36.59439828801611 38.0 37.0 38.0 34.0 38.0 66-67 36.016949899295064 38.0 37.0 38.0 33.0 38.0 68-69 35.47900931520644 38.0 36.0 38.0 31.0 38.0 70-71 35.45748992950655 38.0 36.0 38.0 31.0 38.0 72-73 35.14953423967775 38.0 36.0 38.0 28.5 38.0 74-75 35.70540659617321 38.0 37.0 38.0 31.5 38.0 76 35.14198136958711 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4982945631118483 1101 2 0.5299362779547536 1101 3 0.18639377474008967 1101 4 0.30055735480612356 1101 5 0.24505733669143126 1101 6 -1.0567057139799303 1101 7 0.35544023304269956 1101 8 0.5317920343827822 1101 9 0.5841363056303805 1101 10-11 -0.16692358420586118 1101 12-13 0.4616156115702097 1101 14-15 0.685037927945082 1101 16-17 -0.15276290740845866 1101 18-19 -0.4636826338572533 1101 20-21 -0.10228606874920843 1101 22-23 0.5109644783053184 1101 24-25 -2.005967471559096 1101 26-27 -0.7596391716130739 1101 28-29 0.4914710162832421 1101 30-31 1.0696369075700858 1101 32-33 0.22402169025230023 1101 34-35 0.5357728371128871 1101 36-37 0.3226104591903578 1101 38-39 -0.03990196469011664 1101 40-41 1.3166881419257734 1101 42-43 0.8668360574173803 1101 44-45 0.605608747128727 1101 46-47 0.6437224302294453 1101 48-49 -1.576944066360241 1101 50-51 -1.5530925826398416 1101 52-53 -1.6928666443718399 1101 54-55 -0.6182982329633333 1101 56-57 -1.2739740161708681 1101 58-59 -1.2626727187713627 1101 60-61 -1.4643990466820327 1101 62-63 -0.3763620844233202 1101 64-65 -1.1068430245809324 1101 66-67 -1.3429229445515176 1101 68-69 -0.5518498488945127 1101 70-71 -0.7428331172012932 1101 72-73 -1.245631592531801 1101 74-75 -0.6705837475522927 1101 76 -0.6895212905840964 1102 1 -0.3151112339896045 1102 2 0.08186864510451386 1102 3 0.11996865396714185 1102 4 0.34705493934718135 1102 5 0.24445347195713651 1102 6 0.2325454937495337 1102 7 -0.1367095254114048 1102 8 0.47804807303012353 1102 9 0.5648126341327995 1102 10-11 0.2603107153110429 1102 12-13 0.5570262395895327 1102 14-15 -0.20807801408390958 1102 16-17 0.27115013606979943 1102 18-19 -0.20130340680411507 1102 20-21 0.5426414674826674 1102 22-23 0.5055296956966231 1102 24-25 -4.0793370367764865 1102 26-27 -1.0008831329657326 1102 28-29 0.16115700662140142 1102 30-31 1.2846127529807205 1102 32-33 -0.1440338653032498 1102 34-35 0.3757486825235148 1102 36-37 -2.177993405543937 1102 38-39 -0.3602522062360052 1102 40-41 0.3948886250175647 1102 42-43 0.6201573134560263 1102 44-45 0.6110435297374224 1102 46-47 0.9915485171859686 1102 48-49 -3.5941542112877727 1102 50-51 -0.8033945150069997 1102 52-53 0.10332900780208121 1102 54-55 0.48526456896903625 1102 56-57 0.6055549693363815 1102 58-59 0.31130071118032987 1102 60-61 -5.4423579838801 1102 62-63 -2.75136208442332 1102 64-65 0.07884538121617624 1102 66-67 0.707801693129646 1102 68-69 -1.1369947764307469 1102 70-71 -1.0009852911143327 1102 72-73 -6.011332075623589 1102 74-75 -2.242443650933936 1102 76 -1.4395212905840964 1103 1 0.7069340813808793 1103 2 0.2756496888549407 1103 3 0.061521351796635315 1103 4 0.20933975937848004 1103 5 0.08612183965122 1103 6 0.1805620957771623 1103 7 0.6199074712545851 1103 8 0.30039276922662594 1103 9 0.23284012273591514 1103 10-11 -0.16423170831282619 1103 12-13 -0.8471718960844044 1103 14-15 -0.3562715229632687 1103 16-17 -0.1878210825462432 1103 18-19 0.4879529040076136 1103 20-21 -0.2782403451321471 1103 22-23 0.3885670502587786 1103 24-25 -2.7675387105874663 1103 26-27 -0.6007674631011284 1103 28-29 -0.40337997371472056 1103 30-31 0.6308738947103194 1103 32-33 1.1244222822098422 1103 34-35 0.2640592220894149 1103 36-37 -0.9236898061699108 1103 38-39 -0.33048422637617136 1103 40-41 -0.6521958139835959 1103 42-43 -0.3775633098004221 1103 44-45 0.7949586144485963 1103 46-47 0.7345572944874519 1103 48-49 -2.147430390099778 1103 50-51 -0.43736743465046146 1103 52-53 0.12568050810826747 1103 54-55 0.012889254278960038 1103 56-57 0.46899648801365856 1103 58-59 -0.1749890078099341 1103 60-61 -4.039554690691013 1103 62-63 -1.3527092981404039 1103 64-65 0.12405869005471004 1103 66-67 -0.46866289147946105 1103 68-69 0.6687819126894894 1103 70-71 0.480949797679294 1103 72-73 -4.510175376977607 1103 74-75 -2.172803588336123 1103 76 -2.8828770774793853 1104 1 0.1676569512957471 1104 2 0.3024035132366336 1104 3 0.3154243551436693 1104 4 -0.29303012179050825 1104 5 -0.10279475400962923 1104 6 0.055396428728620606 1104 7 0.5589195340620492 1104 8 0.5165798438273441 1104 9 0.7247645560984495 1104 10-11 -0.7595099166377324 1104 12-13 0.21078349174496225 1104 14-15 0.5940508258023698 1104 16-17 0.29465752113325294 1104 18-19 0.5495922777300137 1104 20-21 -0.13973146088568456 1104 22-23 0.12369071088280492 1104 24-25 0.3599182159799028 1104 26-27 0.5832071982641907 1104 28-29 1.0778294518979692 1104 30-31 0.13589444865600342 1104 32-33 -0.3861691772326239 1104 34-35 -0.41099749781534456 1104 36-37 0.7194015971142278 1104 38-39 0.8197168204149392 1104 40-41 1.3254644057077556 1104 42-43 0.31231920699091376 1104 44-45 0.3425808711145848 1104 46-47 0.8460315627445283 1104 48-49 0.08551432764849665 1104 50-51 0.3249368535989774 1104 52-53 0.302309661016146 1104 54-55 -0.20152321736108547 1104 56-57 -0.07560445095347745 1104 58-59 -0.6688902140906876 1104 60-61 0.35317168350311334 1104 62-63 0.006074986514896352 1104 64-65 0.30712358661107686 1104 66-67 0.07688150728954923 1104 68-69 1.214090446508031 1104 70-71 1.4163228012509634 1104 72-73 0.8080401361971354 1104 74-75 -0.021816325043452878 1104 76 -0.32697708421837746 1105 1 0.8169835115891502 1105 2 0.38106632966898957 1105 3 0.28517753264087276 1105 4 -0.017604874651837576 1105 5 0.1442385280340872 1105 6 0.2226708422353525 1105 7 0.7129148547626798 1105 8 -0.4050735086166384 1105 9 0.6101849553143524 1105 10-11 0.2276009321703114 1105 12-13 -0.47225720972838303 1105 14-15 0.12429764830516632 1105 16-17 0.17921372542050307 1105 18-19 0.575665671065174 1105 20-21 0.4141858714921227 1105 22-23 0.21458926282935664 1105 24-25 0.537594585518093 1105 26-27 0.09933181095731669 1105 28-29 0.5062909741457133 1105 30-31 0.7391446647047388 1105 32-33 0.8006233949065802 1105 34-35 -0.7401082201558324 1105 36-37 0.3618989096787715 1105 38-39 0.005982444426919642 1105 40-41 0.5376347996541782 1105 42-43 0.38940117904159166 1105 44-45 0.6238763203905577 1105 46-47 0.49097391461940987 1105 48-49 -0.4201134783502809 1105 50-51 0.41560354836953906 1105 52-53 0.11597026426635892 1105 54-55 0.5672624833745274 1105 56-57 0.28122374287912066 1105 58-59 -0.3014703629008366 1105 60-61 0.5274966629521387 1105 62-63 -0.1652674665340257 1105 64-65 0.3414068317684311 1105 66-67 0.7336907309895722 1105 68-69 0.19898811318171994 1105 70-71 0.9601332685485673 1105 72-73 0.8674693459857252 1105 74-75 -0.25076474736076193 1105 76 0.195797613840341 1106 1 -1.731864579804732 1106 2 -0.05166347240929525 1106 3 0.23886933122022214 1106 4 -0.04422629403931211 1106 5 -0.10279475400962923 1106 6 0.11281269667119886 1106 7 -0.7664393176125941 1106 8 0.2582066380857171 1106 9 -0.3948526687819296 1106 10-11 0.48929391111346376 1106 12-13 1.1079126783478301 1106 14-15 -0.6164754899871028 1106 16-17 0.19417905223372856 1106 18-19 0.5830851006965219 1106 20-21 0.1401728453344191 1106 22-23 0.2433079357631911 1106 24-25 -1.1520435065081287 1106 26-27 0.6119153322354833 1106 28-29 0.08979117438600781 1106 30-31 0.34163607545026764 1106 32-33 0.022921731858289718 1106 34-35 0.5339785787396778 1106 36-37 0.9347126018989087 1106 38-39 -0.1898525575754917 1106 40-41 0.5957993339374212 1106 42-43 0.852989063450245 1106 44-45 0.7325330242246295 1106 46-47 0.6378975914526634 1106 48-49 1.0615908826724194 1106 50-51 0.1742191502496908 1106 52-53 0.8214484169970007 1106 54-55 -0.09386771496874502 1106 56-57 -0.11388196291520103 1106 58-59 0.6445069150959171 1106 60-61 1.0397745543165087 1106 62-63 0.8314338381895325 1106 64-65 -0.39144100669035 1106 66-67 0.2156374881507901 1106 68-69 1.3887315948333807 1106 70-71 0.2560357199112602 1106 72-73 0.2745473132306273 1106 74-75 -0.21559622934967138 1106 76 -0.5518574669935035 1107 1 0.6672037759063301 1107 2 -0.16518917693894508 1107 3 0.13539779695805976 1107 4 0.21147001521600828 1107 5 0.08256451922351715 1107 6 0.05967524752169595 1107 7 0.6204038415468176 1107 8 0.2469088702379807 1107 9 -0.45589433983982985 1107 10-11 0.5844291260029095 1107 12-13 0.08941679908218703 1107 14-15 0.23442980689547 1107 16-17 0.6417687914997927 1107 18-19 0.46773615564666926 1107 20-21 0.7511902767784662 1107 22-23 0.0846333156927912 1107 24-25 0.6080791670158874 1107 26-27 -0.12313514939510384 1107 28-29 0.847700665775676 1107 30-31 0.5012592021496687 1107 32-33 1.0142797825717764 1107 34-35 0.0726671102406442 1107 36-37 1.0028680726743744 1107 38-39 0.3826344268057653 1107 40-41 0.4429211432665099 1107 42-43 0.7748637341076687 1107 44-45 0.4674886551923194 1107 46-47 0.8213703903903351 1107 48-49 0.4146882837642636 1107 50-51 0.7393920946250461 1107 52-53 0.38909801757032625 1107 54-55 0.4196853908635134 1107 56-57 0.673294227460616 1107 58-59 0.4055780952489414 1107 60-61 0.8512852092076457 1107 62-63 0.5285651325849088 1107 64-65 0.47136277890499656 1107 66-67 0.02003434332436882 1107 68-69 0.994142298203748 1107 70-71 0.28832710114768645 1107 72-73 1.2088790376156808 1107 74-75 -0.17587487951934833 1107 76 0.5305993759548784 1108 1 -0.42387158595647634 1108 2 0.391155222054131 1108 3 -0.06651771594314226 1108 4 0.3465589076011497 1108 5 0.08647814414484856 1108 6 0.06421819906354642 1108 7 0.12795576099301087 1108 8 -0.2409175929464098 1108 9 -0.19342580617085758 1108 10-11 0.45914396237798627 1108 12-13 0.9818019469739454 1108 14-15 0.542957182603466 1108 16-17 -0.08482812479976332 1108 18-19 0.24720634129802477 1108 20-21 0.7592240244184865 1108 22-23 -0.2850370744897148 1108 24-25 0.9143547334098514 1108 26-27 -0.061657805153444656 1108 28-29 -0.6358581762633477 1108 30-31 0.3752463796197816 1108 32-33 0.7202367523641016 1108 34-35 0.5134514085414565 1108 36-37 0.9078200865195569 1108 38-39 0.9465110787881486 1108 40-41 0.8788969928574559 1108 42-43 -0.2966926692906995 1108 44-45 0.6571421322218995 1108 46-47 0.7202946351983854 1108 48-49 0.8731723932670903 1108 50-51 0.7947335043166746 1108 52-53 -0.018371302756925445 1108 54-55 -0.41798767395712133 1108 56-57 0.15877054904652255 1108 58-59 -0.1387402653552101 1108 60-61 0.7820101148086565 1108 62-63 0.8561689714772953 1108 64-65 0.7263285592700015 1108 66-67 0.932892272839787 1108 68-69 1.1697145920992753 1108 70-71 -0.5105932103689881 1108 72-73 0.09743905964211308 1108 74-75 -0.8453702382355175 1108 76 0.6784911317761484 1109 1 -0.522085871670761 1109 2 0.3420480791969922 1109 3 0.35758942691400364 1109 4 0.09209462188686501 1109 5 0.001656715573417955 1109 6 -0.3420318009364536 1109 7 -0.47025852472127383 1109 8 0.1831895499107361 1109 9 0.3556813366862812 1109 10-11 0.36539396237798627 1109 12-13 0.1893912326882301 1109 14-15 0.45143932546060483 1109 16-17 0.5468683037716673 1109 18-19 0.45925991272659417 1109 20-21 0.7860097387042018 1109 22-23 0.281927211224577 1109 24-25 -0.1883238380187251 1109 26-27 0.11021719484655534 1109 28-29 -0.7742510334062018 1109 30-31 0.24578209390548977 1109 32-33 0.34300460950695566 1109 34-35 -0.13833430574425876 1109 36-37 0.7091593722338416 1109 38-39 0.41526107878814855 1109 40-41 -0.03851372142826648 1109 42-43 0.6117894735664464 1109 44-45 0.22187427507903834 1109 46-47 0.46583034948410074 1109 48-49 0.6611188218385209 1109 50-51 -0.11374863854046424 1109 52-53 -0.47819273132835605 1109 54-55 -0.8019162453856907 1109 56-57 -0.40149730809633866 1109 58-59 0.01974187750193579 1109 60-61 0.43826011480865645 1109 62-63 -0.16168817137984348 1109 64-65 0.5388285592700015 1109 66-67 -0.19880415573164356 1109 68-69 -0.7343032650435788 1109 70-71 -0.18470035322612688 1109 72-73 -0.7664002260721787 1109 74-75 -0.979298809664094 1109 76 -0.3884731539381363 1110 1 -0.9136827616229155 1110 2 -0.6210414628399192 1110 3 -0.0817048314591986 1110 4 -0.22125978686227654 1110 5 0.275195676612384 1110 6 0.08888925169512873 1110 7 0.2431300603778368 1110 8 0.46873295387518965 1110 9 0.5812238862419932 1110 10-11 -1.3791271415181114 1110 12-13 0.20839114724735452 1110 14-15 0.5773044143191157 1110 16-17 0.29465752113325294 1110 18-19 -0.6489723155714202 1110 20-21 0.4894551419851325 1110 22-23 -0.5868356049066605 1110 24-25 -1.183143984977029 1110 26-27 0.8296186815177791 1110 28-29 -0.15901265336519543 1110 30-31 -0.21817253698992545 1110 32-33 0.2836872820975245 1110 34-35 0.3162752294573892 1110 36-37 0.9682054248654168 1110 38-39 0.23120007400345344 1110 40-41 -1.62668870434009 1110 42-43 -0.223565960473195 1110 44-45 0.392820105564347 1110 46-47 0.35081625173973663 1110 48-49 1.0639832271700271 1110 50-51 -0.33295788324313236 1110 52-53 0.3166637280017923 1110 54-55 -0.45511173410749706 1110 56-57 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2116 34-35 -1.1161879472008422 2116 36-37 0.4110588820377643 2116 38-39 -3.4799595094471414 2116 40-41 -0.4996481752097779 2116 42-43 -0.6959836356772513 2116 44-45 -0.3501670414475697 2116 46-47 -0.49928694743466195 2116 48-49 -0.0467330689177885 2116 50-51 -0.4099233584284221 2116 52-53 0.2889641314167406 2116 54-55 0.024764426883216117 2116 56-57 0.1645916274778969 2116 58-59 -1.6480627443468094 2116 60-61 -1.0325469580204754 2116 62-63 0.3836549658750599 2116 64-65 -1.9018664687412041 2116 66-67 -0.39737733500334826 2116 68-69 -0.38976544991752604 2116 70-71 0.8822201649811561 2116 72-73 0.2527699419950551 2116 74-75 0.4258570026608339 2116 76 -0.6051223136020027 2117 1 0.6152962038009377 2117 2 0.4013472705716552 2117 3 0.24791961559324704 2117 4 -0.22579621369264657 2117 5 0.131542160317359 2117 6 0.37141159528996326 2117 7 0.49520643484746074 2117 8 0.16027849869779942 2117 9 -1.140022841211291 2117 10-11 -0.6434924931476189 2117 12-13 -1.0003409101689158 2117 14-15 -0.03891613276041994 2117 16-17 -1.0270451059318404 2117 18-19 -0.9709119201574978 2117 20-21 -0.18278237719876245 2117 22-23 0.2708086128418259 2117 24-25 -1.8446159539216893 2117 26-27 -1.6497642741561407 2117 28-29 -1.4828932166946203 2117 30-31 -0.25880853951768756 2117 32-33 -1.6995324524876523 2117 34-35 0.38718793145250885 2117 36-37 -1.407291099464274 2117 38-39 0.38047334293909074 2117 40-41 -0.18069700983796366 2117 42-43 0.11381103690876415 2117 44-45 -0.46688920200990935 2117 46-47 -0.9201619011897506 2117 48-49 -0.8353013264094642 2117 50-51 -0.23946460889087007 2117 52-53 0.5571846271622078 2117 54-55 -0.7250968383776026 2117 56-57 0.3088295113106767 2117 58-59 -0.8497830551126242 2117 60-61 -0.6703567989379806 2117 62-63 -0.5661693034553181 2117 64-65 -0.9363651738836367 2117 66-67 -0.7068482931979432 2117 68-69 0.10093932255750104 2117 70-71 -0.715402846487585 2117 72-73 0.7309885879439975 2117 74-75 0.7022628480178525 2117 76 0.3685180859540509 2118 1 0.11622690859829987 2118 2 0.4125157281399794 2118 3 -0.9661213354178315 2118 4 0.25188802355246764 2118 5 0.3359376252467072 2118 6 0.05679106901870057 2118 7 0.6215496110891081 2118 8 0.5113445787384094 2118 9 -1.5498079330126302 2118 10-11 -0.3793309735605135 2118 12-13 -0.7094216276577043 2118 14-15 0.69174001412172 2118 16-17 -2.274570277266129 2118 18-19 -0.1093859873374683 2118 20-21 0.03175565478363751 2118 22-23 0.6044768588863363 2118 24-25 0.8665588493611551 2118 26-27 -1.919721546344995 2118 28-29 -0.1160791884350374 2118 30-31 -0.5748356991758641 2118 32-33 -0.05367620407407969 2118 34-35 0.24149032590212016 2118 36-37 -2.1625474663311763 2118 38-39 -0.2575247956513138 2118 40-41 -0.07182570092858498 2118 42-43 0.5160976093768284 2118 44-45 -2.07447021563204 2118 46-47 0.15161870630665675 2118 48-49 0.22605115688016042 2118 50-51 -0.35240775128870183 2118 52-53 -0.9047622380548077 2118 54-55 -0.6999783850397634 2118 56-57 0.6553372530823935 2118 58-59 -0.7442115177575133 2118 60-61 -0.32968711453150945 2118 62-63 -0.7964975884202019 2118 64-65 0.4720344369125371 2118 66-67 0.1544449634227405 2118 68-69 -1.367871490540054 2118 70-71 0.15031125792057054 2118 72-73 0.8810012793730522 2118 74-75 -0.3079643285622353 2118 76 -0.09344993164473436 2119 1 0.5408626929225377 2119 2 -0.5684098838925493 2119 3 0.21973057523936035 2119 4 -0.1255660069579747 2119 5 0.19864065268893683 2119 6 -0.5713978296445887 2119 7 -0.43629577694273536 2119 8 0.10988127923404534 2119 9 -0.30872826686805865 2119 10-11 -0.16381613673343054 2119 12-13 -0.03323564701102555 2119 14-15 0.003141734893283399 2119 16-17 -0.2603664023117247 2119 18-19 0.10461620117498427 2119 20-21 -0.8191572982062496 2119 22-23 0.08541319892108845 2119 24-25 1.2594397470803926 2119 26-27 0.4564129398909884 2119 28-29 0.42232705955346717 2119 30-31 0.9229757883689231 2119 32-33 0.597084411284122 2119 34-35 0.5674714017061859 2119 36-37 -0.027009886139367723 2119 38-39 -1.0558812657094592 2119 40-41 -0.4975021014692729 2119 42-43 0.36255844144067595 2119 44-45 0.608131110349035 2119 46-47 0.6091894574813708 2119 48-49 0.12379183961022022 2119 50-51 0.7866593416372538 2119 52-53 0.3836493739348015 2119 54-55 0.27216099316522246 2119 56-57 -0.36268579066639717 2119 58-59 0.24737772849304207 2119 60-61 0.6354683342208176 2119 62-63 0.34339556067757115 2119 64-65 0.26645373015175267 2119 66-67 0.8017618900646681 2119 68-69 0.9341861402879275 2119 70-71 0.3325907438347002 2119 72-73 0.5640209974411547 2119 74-75 -0.06009383700516935 2119 76 0.5486210019060209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 1.0 22 3.0 23 16.0 24 25.0 25 47.0 26 96.0 27 186.0 28 346.0 29 583.0 30 993.0 31 1594.0 32 2520.0 33 3936.0 34 6211.0 35 9841.0 36 17815.0 37 35224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.67598187311178 23.966515609264853 11.90332326283988 28.454179254783483 2 17.216767371601208 19.87537764350453 38.8318227593152 24.076032225579052 3 17.995971802618328 25.235397784491443 30.264350453172206 26.504279959718026 4 13.737411883182277 19.584592145015105 37.52643504531722 29.1515609264854 5 13.358509566968783 35.67724068479355 37.01283987915408 13.951409869083587 6 27.647280966767372 38.987915407854985 18.07653575025176 15.28826787512588 7 26.81143001007049 29.098690835850956 23.59264853977845 20.4972306143001 8 23.077794561933533 36.492950654582074 20.90508559919436 19.52416918429003 9 22.99093655589124 16.6012084592145 23.30438066465257 37.103474320241695 10-11 24.954053373615306 26.11593655589124 27.35649546827795 21.573514602215507 12-13 24.16729607250755 23.11492950654582 27.189702920443104 25.528071500503525 14-15 21.312311178247732 25.11581067472306 24.158484390735147 29.41339375629406 16-17 22.870720040281974 28.406344410876134 25.528071500503525 23.194864048338367 18-19 22.44146525679758 27.55601711983887 26.665407854984895 23.33710976837865 20-21 21.64149043303122 27.226837865055387 26.441968781470294 24.689702920443104 22-23 22.505664652567976 27.822255790533735 26.80513595166163 22.866943605236656 24-25 22.741062437059416 28.59453675730111 26.69813695871098 21.9662638469285 26-27 22.230614300100704 28.92119838872105 26.26007049345418 22.588116817724067 28-29 21.615055387713998 28.232628398791544 27.613293051359516 22.539023162134946 30-31 21.47721550855992 28.356621349446122 27.18277945619335 22.983383685800604 32-33 21.606873111782477 29.31520644511581 26.786253776435043 22.291666666666668 34-35 22.694486404833835 27.232502517623363 28.566842900302113 21.506168177240685 36-37 22.870090634441087 27.63280463242699 27.018504531722055 22.47860020140987 38-39 21.788141993957705 27.336354481369586 27.74358006042296 23.13192346424975 40-41 21.238041289023162 25.991943605236656 29.26737160120846 23.502643504531722 42-43 22.34264853977845 25.964879154078552 28.67384189325277 23.018630412890232 44-45 20.47205438066465 25.98816717019134 29.718655589123866 23.821122860020143 46-47 21.90458207452165 25.020140986908356 29.05652064451158 24.018756294058406 48-49 21.00956696878147 24.704179254783483 29.770896273917423 24.515357502517624 50-51 19.58207452165156 25.188192346424977 30.761581067472303 24.468152064451157 52-53 18.828675730110774 25.80375125881168 32.14564451158107 23.221928499496475 54-55 18.457326283987914 26.204053373615306 30.633811681772404 24.70480866062437 56-57 19.27995971802618 25.839627391742198 29.61858006042296 25.26183282980866 58-59 18.398791540785496 26.764224572004032 30.163016112789524 24.673967774420944 60-61 19.486404833836858 27.18152064451158 28.592019133937562 24.740055387713998 62-63 18.0702416918429 28.822381671701912 27.660498489425983 25.446878147029206 64-65 18.77643504531722 29.485146022155085 25.836480362537767 25.90193856998993 66-67 19.0464501510574 29.32527693856999 25.285120845921448 26.343152064451157 68-69 18.773917421953676 29.146525679758305 25.45883685800604 26.620720040281974 70-71 19.12323766364552 29.116943605236656 24.796701913393758 26.96311681772407 72-73 19.011203423967775 28.834969788519636 24.51787512588117 27.63595166163142 74-75 19.497104733131923 29.476334340382675 23.93189828801611 27.094662638469284 76 19.75453172205438 30.040281973816718 23.406344410876134 26.798841893252767 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.5 15 3.0 16 3.0 17 5.0 18 7.5 19 9.5 20 27.0 21 45.0 22 48.0 23 67.5 24 106.0 25 128.0 26 185.5 27 252.5 28 349.5 29 437.0 30 535.0 31 753.0 32 1103.0 33 1333.0 34 1454.5 35 1806.5 36 2146.0 37 2255.0 38 2758.0 39 3794.0 40 4807.5 41 5539.0 42 5790.0 43 5725.5 44 5780.5 45 5954.0 46 6008.0 47 5965.5 48 5564.5 49 4908.0 50 4610.0 51 4393.0 52 3647.0 53 2868.0 54 2618.0 55 2426.5 56 1909.5 57 1368.5 58 1153.0 59 960.0 60 673.5 61 484.5 62 389.0 63 337.0 64 241.0 65 166.5 66 121.5 67 107.0 68 82.5 69 44.5 70 27.5 71 24.0 72 19.0 73 9.0 74 3.0 75 2.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 79440.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.60297079556898 #Duplication Level Percentage of deduplicated Percentage of total 1 56.95241047899551 23.124370594159114 2 16.760192218260737 13.610271903323262 3 9.055960316230042 11.030966767371602 4 5.478220430940939 8.897280966767372 5 3.2522089598511856 6.602467270896274 6 2.120601457138428 5.166163141993958 7 1.3889319485351108 3.947633434038268 8 1.0634010230971942 3.454179254783484 9 0.8556812897225238 3.1268882175226587 >10 3.041388931948535 18.246475327291037 >50 0.01860176716788095 0.4695367573011078 >100 0.009300883583940475 0.959214501510574 >500 0.0 0.0 >1k 0.0031002945279801583 1.3645518630412892 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 1084 1.3645518630412892 TruSeq Adapter, Index 5 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 474 0.5966767371601208 TruSeq Adapter, Index 5 (96% over 26bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 151 0.19008056394763342 RNA PCR Primer, Index 5 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 137 0.17245720040281975 No Hit TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 81 0.10196374622356497 TruSeq Adapter, Index 5 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0037764350453172208 0.0 2 0.0 0.0 0.0 0.0037764350453172208 0.0 3 0.0 0.0 0.0 0.005035246727089627 0.0 4 0.0 0.0 0.0 0.008811681772406849 0.0 5 0.0 0.0 0.0 0.010070493454179255 0.0 6 0.0 0.0 0.0 0.010070493454179255 0.0 7 0.0 0.0 0.0 0.010070493454179255 0.0 8 0.0 0.0 0.0 0.013846928499496475 0.0 9 0.0 0.0 0.0 0.026435045317220542 0.0 10 0.0 0.0 0.0 0.036505538771399795 0.0 11 0.0 0.0 0.0 0.04783484390735146 0.0 12 0.0 0.0 0.0 0.052870090634441085 0.0 13 0.0 0.0 0.0 0.05412890231621349 0.0 14 0.0 0.0 0.0 0.06797583081570997 0.0 15 0.0 0.0 0.0 0.07049345417925479 0.0 16 0.0 0.0 0.0 0.08434038267875126 0.0 17 0.0 0.0 0.0 0.0918932527693857 0.0 18 0.0 0.0 0.0 0.09566968781470292 0.0 19 0.0 0.0 0.0 0.10448136958710977 0.0 20 0.0 0.0 0.0 0.10699899295065458 0.0 21 0.0 0.0 0.0 0.1107754279959718 0.0 22 0.0 0.0 0.0 0.11203423967774422 0.0 23 0.0 0.0 0.0 0.12336354481369587 0.0 24 0.0 0.0 0.0 0.13217522658610273 0.0 25 0.0 0.0 0.0 0.13469284994964753 0.0 26 0.0 0.0 0.0 0.14224572004028196 0.0 27 0.0 0.0 0.0 0.17119838872104734 0.0 28 0.0 0.0 0.0 0.2039274924471299 0.0 29 0.0 0.0 0.0 0.23288016112789528 0.0 30 0.0 0.0 0.0 0.2719033232628399 0.0 31 0.0 0.0 0.0 0.3159617321248741 0.0 32 0.0 0.0 0.0 0.35246727089627394 0.0 33 0.0 0.0 0.0 0.38393756294058407 0.0 34 0.0 0.0 0.0 0.44561933534743203 0.0 35 0.0 0.0 0.0 0.5689828801611279 0.0 36 0.0 0.0 0.0 0.7477341389728097 0.0 37 0.0 0.0 0.0 1.1455186304128901 0.0 38 0.0 0.0 0.0 1.5546324269889225 0.0 39 0.0 0.0 0.0 2.045568982880161 0.0 40 0.0 0.0 0.0 2.769385699899295 0.0 41 0.0 0.0 0.0 3.6341893252769384 0.0 42 0.0 0.0 0.0 4.675226586102719 0.0 43 0.0 0.0 0.0 5.77920443101712 0.0 44 0.0 0.0 0.0 6.840382678751259 0.0 45 0.0 0.0 0.0 7.914149043303122 0.0 46 0.0 0.0 0.0 9.013091641490433 0.0 47 0.0 0.0 0.0 10.05287009063444 0.0 48 0.0 0.0 0.0 10.945367573011078 0.0 49 0.0 0.0 0.0 11.771148036253777 0.0 50 0.0 0.0 0.0 12.527693856998994 0.0 51 0.0 0.0 0.0 13.439073514602216 0.0 52 0.0 0.0 0.0 14.526686807653576 0.0 53 0.0 0.0 0.0 15.52744209466264 0.0 54 0.0 0.0 0.0 16.550855991943607 0.0 55 0.0 0.0 0.0 17.47985901309164 0.0 56 0.0 0.0 0.0 18.329556898288015 0.0 57 0.0 0.0 0.0 19.13645518630413 0.0 58 0.0 0.0 0.0 19.921953675730112 0.0 59 0.0 0.0 0.0 20.53877139979859 0.0 60 0.0 0.0 0.0 21.232376636455186 0.0 61 0.0 0.0 0.0 21.96122860020141 0.0 62 0.0 0.0 0.0 22.648539778449145 0.0 63 0.0 0.0 0.0 23.37865055387714 0.0 64 0.0 0.0 0.0 24.098690835850956 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCCG 30 8.308765E-6 58.333332 5 CGTGCGA 25 2.3774595E-4 55.999996 10 GTTTAGT 25 2.3774595E-4 55.999996 53 CCGTGCG 25 2.3774595E-4 55.999996 9 CCCGTGC 25 2.3774595E-4 55.999996 8 TATAGTC 25 2.3774595E-4 55.999996 5 AATTTAG 20 0.006928344 52.500004 16 ATGGCTC 20 0.006928344 52.500004 7 ACCTATG 20 0.006928344 52.500004 2 ATGGTTC 20 0.006928344 52.500004 16 TTTTCCG 20 0.006928344 52.500004 26 TCTATCC 20 0.006928344 52.500004 17 AGCCCGT 40 4.5560824E-5 43.750004 6 GCGAGAA 35 0.001248723 40.0 13 AGTCATA 35 0.001248723 40.0 45 GTGCGAG 35 0.001248723 40.0 11 CCGTCTT 325 0.0 38.769234 47 CGTATGC 335 0.0 38.656715 41 CGTCTTC 320 0.0 38.281254 48 TATGCCG 340 0.0 38.088234 43 >>END_MODULE