FastQCFastQC Report
Wed 25 May 2016
SRR1780666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132223
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACG150.00222130169.993954
ATAACGC150.00222130169.993955
AGCAACT200.00694087552.49546458
TCGTTGT200.00694087552.49546434
GCCTGGG200.00694087552.49546454
AACGCTC200.00694087552.4954647
CTGCAAT200.00694087552.49546428
CATCGTT200.00694087552.49546432
CATCGCC200.00694087552.49546411
GCTCGGT200.00694087552.49546412
TCGGTAG200.00694087552.49546414
TGCCGAG200.00694087552.49546457
CGGATCG404.5750006E-543.7462220
CGCGGTC350.001252274839.99654439
TCGTGCC350.001252274839.99654426
GTGCCAG350.001252274839.99654428
GGATCGG459.162447E-538.8855321
TACTGAC556.658307E-638.17851634
GACTATC556.658307E-638.17851638
ACCATTA400.002412745534.99697559
Produced by FastQC (version 0.11.5)