##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780666_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 132223 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.258449740211613 34.0 31.0 34.0 12.0 34.0 2 31.690553080780198 34.0 33.0 34.0 23.0 34.0 3 31.877056185383783 34.0 34.0 34.0 23.0 34.0 4 32.17218638209691 34.0 34.0 34.0 27.0 34.0 5 32.14865038609017 34.0 34.0 34.0 27.0 34.0 6 35.21304916693767 38.0 37.0 38.0 27.0 38.0 7 34.357978566512635 38.0 35.0 38.0 24.0 38.0 8 33.345560152167174 37.0 34.0 38.0 21.0 38.0 9 33.93003486534113 37.0 34.0 38.0 23.0 38.0 10-11 34.435567185739245 37.5 34.5 38.0 25.0 38.0 12-13 34.86859699144627 38.0 36.0 38.0 26.5 38.0 14-15 34.419548792570126 38.0 34.5 38.0 25.0 38.0 16-17 34.28377816264947 38.0 34.0 38.0 24.0 38.0 18-19 34.257712349591216 38.0 34.5 38.0 24.5 38.0 20-21 34.35702941243203 38.0 34.5 38.0 24.5 38.0 22-23 34.31726325979595 38.0 34.5 38.0 24.0 38.0 24-25 34.74156538574983 38.0 35.5 38.0 26.5 38.0 26-27 34.80666752380448 38.0 36.0 38.0 26.5 38.0 28-29 34.96925648336523 38.0 36.0 38.0 27.0 38.0 30-31 34.78910250107772 38.0 36.0 38.0 26.0 38.0 32-33 34.516562927781095 38.0 35.0 38.0 25.0 38.0 34-35 34.35690084175975 38.0 34.5 38.0 24.0 38.0 36-37 34.37511249933824 38.0 34.5 38.0 25.0 38.0 38-39 34.94145118474093 38.0 36.0 38.0 27.0 38.0 40-41 34.431641998744546 38.0 35.0 38.0 24.5 38.0 42-43 34.77412401775788 38.0 36.0 38.0 26.5 38.0 44-45 33.80881162883915 37.0 34.0 38.0 22.5 38.0 46-47 34.56094628014793 37.5 35.0 38.0 26.0 38.0 48-49 34.27603745188054 37.0 34.0 38.0 24.0 38.0 50-51 34.26647784424797 37.0 34.5 38.0 25.0 38.0 52-53 34.2922260121159 37.0 34.0 38.0 24.5 38.0 54-55 34.0281342882857 37.0 34.0 38.0 24.0 38.0 56-57 34.11909047593838 37.0 34.5 38.0 24.0 38.0 58-59 33.99705043751844 37.0 34.0 38.0 24.0 38.0 60-61 34.00108150624324 37.0 34.0 38.0 23.5 38.0 62-63 34.10993170628408 37.0 34.0 38.0 24.0 38.0 64-65 34.123397593459536 37.0 34.0 38.0 24.0 38.0 66-67 34.05018037709022 37.0 34.0 38.0 24.0 38.0 68-69 33.612245978385005 37.0 33.5 38.0 22.5 38.0 70-71 33.45325321615755 37.0 33.5 38.0 22.0 38.0 72-73 33.520730130158896 37.0 33.5 38.0 22.5 38.0 74-75 33.09791412991688 37.0 32.0 38.0 21.0 38.0 76 32.349311390605266 36.0 31.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.3625630580001484 1101 2 -0.7565371711719706 1101 3 0.24513559886140968 1101 4 -2.2863491225991375 1101 5 -0.30141974050664544 1101 6 -2.3859177535693306 1101 7 -1.0231763810960217 1101 8 -0.9798065359683577 1101 9 -1.062417777178581 1101 10-11 -0.7833620723931958 1101 12-13 -0.7953869472837525 1101 14-15 0.03350980571894979 1101 16-17 -2.082921696887148 1101 18-19 -0.04552768792798645 1101 20-21 -1.176506577101172 1101 22-23 -0.32259531185049894 1101 24-25 -0.8939006325580792 1101 26-27 -1.0198177108988276 1101 28-29 -0.2151344802704429 1101 30-31 -1.4092236135559375 1101 32-33 0.2469093481019371 1101 34-35 -0.7969120001280388 1101 36-37 0.26744965488858696 1101 38-39 -0.6781827842241412 1101 40-41 -0.020652936336169603 1101 42-43 -0.6595474900809535 1101 44-45 -0.15286313462442536 1101 46-47 0.2807378104435827 1101 48-49 -0.8580550387862331 1101 50-51 -0.7734294836518316 1101 52-53 -0.24615729018552912 1101 54-55 -1.2180262683775922 1101 56-57 -0.41585072518895316 1101 58-59 -0.25393784654356466 1101 60-61 0.5254644233662376 1101 62-63 -1.1474653441807163 1101 64-65 -0.11646004310122748 1101 66-67 -0.4846304176335394 1101 68-69 0.5178500781602366 1101 70-71 0.5203259803634452 1101 72-73 -0.5906664297719999 1101 74-75 -1.7073736085278632 1101 76 -2.350888366163989 1102 1 0.9613074239275612 1102 2 0.058598370996705285 1102 3 0.8285316831987615 1102 4 0.5482517810153169 1102 5 1.0916525486499822 1102 6 0.9295189934186183 1102 7 2.156605245409999 1102 8 2.5313003917424837 1102 9 1.1462418613756356 1102 10-11 0.6993374757995738 1102 12-13 -3.5466105918620663 1102 14-15 -0.734901338859359 1102 16-17 -1.829853172790763 1102 18-19 0.20366282412020098 1102 20-21 0.3360687241036473 1102 22-23 -0.04281368534447694 1102 24-25 1.330026325273245 1102 26-27 -4.984482620537378 1102 28-29 0.6486268149102727 1102 30-31 0.8012056033115371 1102 32-33 -6.389348182018544 1102 34-35 -0.020048295308754405 1102 36-37 1.1249609500693012 1102 38-39 1.5283495952939248 1102 40-41 1.3831121239048016 1102 42-43 1.236141515943146 1102 44-45 -4.881703496070205 1102 46-47 0.9393786236965838 1102 48-49 1.0322499310932827 1102 50-51 -6.036193037868699 1102 52-53 -1.0439547299445664 1102 54-55 1.325366050899511 1102 56-57 -0.39150961073112 1102 58-59 1.7211751052636686 1102 60-61 1.1886421342096085 1102 62-63 -3.8052213682771026 1102 64-65 0.40110396292287476 1102 66-67 0.7858891606797158 1102 68-69 -8.102725976056632 1102 70-71 0.39225745626706043 1102 72-73 1.2458734798665532 1102 74-75 -5.947471500094128 1102 76 -1.186807040862785 1103 1 -0.39408187520444926 1103 2 -0.2447764184837986 1103 3 -0.20026090651493433 1103 4 0.3902973827772058 1103 5 0.26175230250410664 1103 6 -0.8389419471177177 1103 7 1.0314271135276343 1103 8 1.4215711522036827 1103 9 0.45512254540206953 1103 10-11 0.9073469329831454 1103 12-13 -1.5778970279289126 1103 14-15 -0.3065103555713691 1103 16-17 -0.438886078607581 1103 18-19 -0.3152353492183124 1103 20-21 -0.4307404480689172 1103 22-23 -0.31584127959244057 1103 24-25 0.36996025453869663 1103 26-27 -0.3446328990708736 1103 28-29 0.4403258691919234 1103 30-31 0.31184493483116427 1103 32-33 -5.4973446841561255 1103 34-35 -0.9018011130312686 1103 36-37 0.07526215488858412 1103 38-39 0.6810410060984324 1103 40-41 0.5991051281799642 1103 42-43 0.7139236120695855 1103 44-45 -5.387338941076038 1103 46-47 -0.19713517342739095 1103 48-49 0.07747923540731705 1103 50-51 -0.05935018257656566 1103 52-53 -0.34812301599198037 1103 54-55 0.3480859628052073 1103 56-57 1.0331244092196528 1103 58-59 0.5413914545317056 1103 60-61 0.8397789394952682 1103 62-63 0.6358007848515399 1103 64-65 0.7135634784041542 1103 66-67 0.1475940447320525 1103 68-69 -2.937711077753743 1103 70-71 0.3749462760623743 1103 72-73 0.9635506401204736 1103 74-75 -1.5248131246568946 1103 76 0.054286365018803906 1104 1 -1.3811436813063835 1104 2 0.2609933166329057 1104 3 0.4447036882923072 1104 4 0.27311056574774284 1104 5 0.16233364702723208 1104 6 0.18872655537375493 1104 7 1.7502399468172598 1104 8 1.964341499261998 1104 9 0.07906257512494363 1104 10-11 0.1201059154116777 1104 12-13 -0.03588068034608227 1104 14-15 0.8263028273991679 1104 16-17 0.14289554566028073 1104 18-19 1.0011821697955838 1104 20-21 1.6351634770993684 1104 22-23 1.0067830756833729 1104 24-25 0.7716015693389409 1104 26-27 1.129534423247513 1104 28-29 0.21376541810354155 1104 30-31 -0.36173885745837 1104 32-33 -0.04896632940483414 1104 34-35 0.5096640500074656 1104 36-37 0.46821608442787976 1104 38-39 -0.10097665278782841 1104 40-41 1.041101806211266 1104 42-43 1.0351383161521142 1104 44-45 -2.7764573080661563 1104 46-47 0.681317925619723 1104 48-49 1.0386209435985876 1104 50-51 1.7199902317953217 1104 52-53 0.7915417274296388 1104 54-55 -0.0588333483233896 1104 56-57 0.690174850691804 1104 58-59 0.739769809282997 1104 60-61 0.5746006022280241 1104 62-63 0.9391708346810788 1104 64-65 0.8003717658418665 1104 66-67 0.6376172110927456 1104 68-69 -3.1090765817313617 1104 70-71 0.09193421207076824 1104 72-73 1.2344987125044256 1104 74-75 -0.6661388524303007 1104 76 0.6975025552452223 1105 1 -0.6353024197022776 1105 2 0.292000062870585 1105 3 -0.026473443691781284 1105 4 0.17395672846468813 1105 5 0.4007079190678198 1105 6 0.4971939485583263 1105 7 1.3461720231592977 1105 8 0.9507120810529202 1105 9 1.3417045632469566 1105 10-11 1.3997828744153153 1105 12-13 -0.8061914685603497 1105 14-15 0.01881565678278463 1105 16-17 1.3641195265171078 1105 18-19 0.9223247056890287 1105 20-21 0.04596682715414602 1105 22-23 0.7039339434686482 1105 24-25 0.5088586759525597 1105 26-27 0.5610665976118128 1105 28-29 -0.640433682398104 1105 30-31 0.2796726630398112 1105 32-33 0.7302205183147024 1105 34-35 0.9152887977443029 1105 36-37 0.8744642825481606 1105 38-39 0.41486907747798085 1105 40-41 0.6479640849404333 1105 42-43 1.0130256482169244 1105 44-45 1.7645570781415358 1105 46-47 0.9783109487414521 1105 48-49 0.699690971852057 1105 50-51 0.2909987078375309 1105 52-53 -0.25782617316425416 1105 54-55 -0.21387068327121028 1105 56-57 0.41982081736423993 1105 58-59 0.6563680045202744 1105 60-61 0.6149591042172986 1105 62-63 0.7853804005001308 1105 64-65 0.463892350515799 1105 66-67 0.7191594759834814 1105 68-69 1.6114338547559868 1105 70-71 1.7780719910017453 1105 72-73 1.108568942568425 1105 74-75 -0.45424860852786253 1105 76 -1.0542128342490962 1106 1 0.7303198899767303 1106 2 0.5619238114869916 1106 3 -0.5000775514276086 1106 4 0.08751287162051824 1106 5 0.4796496236552059 1106 6 0.11621374932084194 1106 7 1.4527087345109138 1106 8 1.600124822413143 1106 9 1.540942049411015 1106 10-11 0.5453138669131619 1106 12-13 0.4848243966468857 1106 14-15 0.33482844733745054 1106 16-17 0.9027448493556278 1106 18-19 -0.07852082376614078 1106 20-21 0.01702665989304819 1106 22-23 0.11167130664660618 1106 24-25 1.0138396131066614 1106 26-27 0.6010908873670715 1106 28-29 -0.8613320958773798 1106 30-31 -0.5194295384114227 1106 32-33 0.6936398394314196 1106 34-35 1.12430367906272 1106 36-37 0.8252621548885841 1106 38-39 0.34512827068915186 1106 40-41 0.08707827753666919 1106 42-43 0.6972895763930325 1106 44-45 1.4616961139304863 1106 46-47 0.669800310443577 1106 48-49 0.9418275479189688 1106 50-51 0.6053819325331347 1106 52-53 0.06752587455435588 1106 54-55 0.29077972873223956 1106 56-57 0.9692919771231843 1106 58-59 0.378811430913089 1106 60-61 0.952379163250626 1106 62-63 0.9975852338539681 1106 64-65 1.106471676551962 1106 66-67 1.241796605487849 1106 68-69 1.426854774692032 1106 70-71 0.956624752039744 1106 72-73 1.0425254054881137 1106 74-75 -1.0174711518804642 1106 76 -0.4524418343720811 1107 1 0.3521380988121905 1107 2 0.3201819804989796 1107 3 0.42624903073801335 1107 4 0.35954580028003846 1107 5 0.38399157054733735 1107 6 0.5958785189242377 1107 7 0.5891709839425374 1107 8 1.3214868444943662 1107 9 -0.48316006509631393 1107 10-11 0.1471609031852097 1107 12-13 0.7069628277805151 1107 14-15 0.036988276155973665 1107 16-17 0.8267384894881715 1107 18-19 1.3103969586015722 1107 20-21 1.1515943226417562 1107 22-23 -0.146880337557441 1107 24-25 -0.03534905800794519 1107 26-27 0.5594359844739216 1107 28-29 -0.8654035998077205 1107 30-31 -0.059713652116343496 1107 32-33 1.038064553757465 1107 34-35 0.965704484447798 1107 36-37 0.028989661315314663 1107 38-39 0.837936271045784 1107 40-41 1.000009017391342 1107 42-43 0.5489880176311317 1107 44-45 0.37996141550411267 1107 46-47 0.7025766600579715 1107 48-49 0.7649325897484758 1107 50-51 1.2754378042659056 1107 52-53 0.972685094133233 1107 54-55 0.572683082522154 1107 56-57 1.1627924303380368 1107 58-59 1.3289429375181356 1107 60-61 0.04283267529425672 1107 62-63 0.07963941160334542 1107 64-65 0.6329736008576461 1107 66-67 1.1163898265823988 1107 68-69 1.3261927838157703 1107 70-71 0.012336745144935435 1107 72-73 0.16564382087067742 1107 74-75 0.6371716999554309 1107 76 -0.7571062324878959 1108 1 -1.0654797246668153 1108 2 -0.24378717117197013 1108 3 -0.3975727344719253 1108 4 -0.43305745593247025 1108 5 -0.1364197405066463 1108 6 -0.11683442023600321 1108 7 0.8041152855706457 1108 8 0.8368184640316443 1108 9 -0.27616777717857843 1108 10-11 -0.138007905726532 1108 12-13 0.14209221938291705 1108 14-15 0.3054681390522873 1108 16-17 -0.8313591968871492 1108 18-19 -0.6972568545946558 1108 20-21 0.22974342289882088 1108 22-23 -1.0533036451838385 1108 24-25 -1.8465881325580753 1108 26-27 -1.0072135442321581 1108 28-29 -0.01007198027044609 1108 30-31 0.15331805311073765 1108 32-33 0.22945101476860685 1108 34-35 -0.46268283346137196 1108 36-37 -0.3407378451114127 1108 38-39 -0.04145361755747956 1108 40-41 -0.11715293633616142 1108 42-43 0.37118167658571366 1108 44-45 -0.07377980129108863 1108 46-47 0.2851336437769092 1108 48-49 -0.12428420545290209 1108 50-51 -0.1648878169851571 1108 52-53 -0.16688645685220393 1108 54-55 -0.06483876837759084 1108 56-57 0.20525344147770852 1108 58-59 -1.5431461798768922 1108 60-61 0.3473810900329042 1108 62-63 -0.3080486775140514 1108 64-65 0.13081079023211117 1108 66-67 -0.14925541763354033 1108 68-69 0.8855792448269 1108 70-71 1.2987634803634478 1108 72-73 -0.07906226310533526 1108 74-75 0.7409180581388028 1108 76 0.22402830050268108 1109 1 -0.39374845851694573 1109 2 -0.9996734760815329 1109 3 0.6344686091973273 1109 4 0.09109758282721714 1109 5 0.24218491065614245 1109 6 0.4710105410043113 1109 7 -1.2300966007342637 1109 8 0.6375626500781522 1109 9 0.37974953548292234 1109 10-11 0.8788189676584821 1109 12-13 0.07708963540358837 1109 14-15 0.11715806153290487 1109 16-17 -0.4205064837088557 1109 18-19 0.1530067112968112 1109 20-21 0.9191491076533538 1109 22-23 0.7455490679944532 1109 24-25 0.9151069578811999 1109 26-27 0.3676701766980699 1109 28-29 0.0514473995745135 1109 30-31 0.08384001693502796 1109 32-33 0.4425569579210631 1109 34-35 0.07325515103475766 1109 36-37 0.2638926458446562 1109 38-39 0.29556963825647387 1109 40-41 -0.008088336852956957 1109 42-43 0.303998214053415 1109 44-45 -0.08521132583889113 1109 46-47 -0.5556518859388504 1109 48-49 -0.3974728359438515 1109 50-51 0.8853964207409319 1109 52-53 0.3736303390134381 1109 54-55 0.38882531430974865 1109 56-57 0.23271597377745223 1109 58-59 -0.34751827290014603 1109 60-61 0.24870408744892814 1109 62-63 -0.5727721917259316 1109 64-65 -0.25455355085316 1109 66-67 0.14477042215973768 1109 68-69 1.434819554904422 1109 70-71 -0.030332126871698506 1109 72-73 -0.8876100667229068 1109 74-75 1.353083432815808 1109 76 -0.42306213877380827 1110 1 0.5770773529587565 1110 2 0.21769228088282233 1110 3 -0.20670515456324878 1110 4 0.3522210828803125 1110 5 0.4728953279865067 1110 6 0.21262676697860883 1110 7 0.5434760161642558 1110 8 2.027941751702876 1110 9 -1.5424691470415937 1110 10-11 -1.3848389559548409 1110 12-13 -0.34682102262621584 1110 14-15 -0.03750902989748539 1110 16-17 -0.2930030325035915 1110 18-19 0.10239611344186983 1110 20-21 -0.1825853442244636 1110 22-23 0.3187146196563404 1110 24-25 0.453466661962473 1110 26-27 -1.0859350054193726 1110 28-29 0.21776363616790917 1110 30-31 0.4105965919235217 1110 32-33 1.1407021563211188 1110 34-35 0.1943765272692204 1110 36-37 -0.08912140675524682 1110 38-39 0.5605372500224277 1110 40-41 0.46273747462274173 1110 42-43 0.592186242795762 1110 44-45 1.3559553585262591 1110 46-47 0.39788250222439814 1110 48-49 0.6410673927206147 1110 50-51 0.007349625937209225 1110 52-53 -0.16432937922662916 1110 54-55 -0.41042780947348234 1110 56-57 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0.20451500991904936 2116 44-45 1.8441509603310635 2116 46-47 0.20169942023586174 2116 48-49 0.66664358881021 2116 50-51 0.5051220742116698 2116 52-53 0.9750917825147596 2116 54-55 -0.35603164671586995 2116 56-57 -0.12483162281506566 2116 58-59 -0.822548751588073 2116 60-61 -1.3652440336070555 2116 62-63 0.8539256053741795 2116 64-65 1.1291213738127226 2116 66-67 -0.06739191614985884 2116 68-69 0.28614106480712564 2116 70-71 -2.951562929132102 2116 72-73 0.4776894678541126 2116 74-75 -1.4622233859759355 2116 76 -1.7685256065200718 2117 1 -0.1696902509826046 2117 2 0.17712510952978278 2117 3 0.6094448093877247 2117 4 0.06158581892132986 2117 5 -0.6150162317347139 2117 6 -1.2714075196512056 2117 7 -2.4234870536106428 2117 8 -2.7660821207636808 2117 9 0.19880298305533728 2117 10-11 -0.5163938706388151 2117 12-13 0.6312852018390558 2117 14-15 -0.25656694866701457 2117 16-17 -0.09013112671171086 2117 18-19 -0.015291942313950813 2117 20-21 -0.18750803908947944 2117 22-23 0.058380565342481816 2117 24-25 0.5221721013600487 2117 26-27 0.5988683271128679 2117 28-29 0.17129644078218575 2117 30-31 -0.0844246369477446 2117 32-33 1.2088428276341006 2117 34-35 -0.1742968685490922 2117 36-37 -1.7010536345851008 2117 38-39 0.19085047600977845 2117 40-41 -1.5720418252250496 2117 42-43 -1.0206311889113593 2117 44-45 0.4888400817498493 2117 46-47 -0.47347454335758954 2117 48-49 -0.05376958556986011 2117 50-51 0.7572174461727315 2117 52-53 0.9950433677092079 2117 54-55 -1.1183826280267155 2117 56-57 -1.2519161491655666 2117 58-59 -0.9424795132102304 2117 60-61 0.6438489262902181 2117 62-63 -0.411171484531593 2117 64-65 -0.294265233159706 2117 66-67 -1.9015712071072244 2117 68-69 -0.4694032113134483 2117 70-71 0.517301492059353 2117 72-73 0.5974640526841384 2117 74-75 -0.3566608892296159 2117 76 -0.9355740386786096 2118 1 -0.003170599899590343 2118 2 0.3798105941911558 2118 3 0.0882745653418553 2118 4 0.29868928522208904 2118 5 0.033077466197262595 2118 6 0.46453615704518825 2118 7 0.9498508535408519 2118 8 -4.506779301331488 2118 9 -1.5539331403070733 2118 10-11 -1.1242052986501818 2118 12-13 0.6251350499229531 2118 14-15 -1.0917869819905448 2118 16-17 -0.4375044482837964 2118 18-19 -1.025092981224084 2118 20-21 -2.8744465212352495 2118 22-23 -0.2788976861521846 2118 24-25 -0.8089233280888024 2118 26-27 0.3337175544643003 2118 28-29 -0.8403177903263099 2118 30-31 -0.8387452616006215 2118 32-33 -0.22423241167459906 2118 34-35 0.6323041311568787 2118 36-37 -0.5015534875695167 2118 38-39 -0.3998744741682785 2118 40-41 -1.441532824604316 2118 42-43 -1.0985576157792707 2118 44-45 -0.1915153692612961 2118 46-47 -0.830199689556423 2118 48-49 -1.3459303879482505 2118 50-51 -0.24596789147305742 2118 52-53 -1.6395493991240784 2118 54-55 -0.05474938290273457 2118 56-57 0.6242068865428934 2118 58-59 0.590108941165937 2118 60-61 -1.8938442358516454 2118 62-63 0.46793270051202285 2118 64-65 -0.15845550399507147 2118 66-67 -2.792853182996666 2118 68-69 0.9898734720149847 2118 70-71 -0.956096854832083 2118 72-73 -1.982242896252444 2118 74-75 -0.06912291020383776 2118 76 -0.4253646231472281 2119 1 -0.16663914495667242 2119 2 0.0227718350392081 2119 3 0.09849351811606866 2119 4 0.10241252336359707 2119 5 0.028549203592731942 2119 6 0.4224285197225939 2119 7 -1.4757190829593725 2119 8 0.614234613099967 2119 9 -0.021385168482929373 2119 10-11 -0.1693578022068678 2119 12-13 -0.15481047212847443 2119 14-15 -1.1590950079456448 2119 16-17 -0.7692473956449106 2119 18-19 -1.5653355295428923 2119 20-21 -1.6093870118837827 2119 22-23 0.2425638496401774 2119 24-25 0.05141186744192083 2119 26-27 0.010840285995584509 2119 28-29 0.4009466532699264 2119 30-31 -0.251944886847852 2119 32-33 -0.24363801214651204 2119 34-35 0.17173124521357863 2119 36-37 -0.8564148637449591 2119 38-39 -0.7746792904353228 2119 40-41 -0.6396746754666012 2119 42-43 -0.7970377851120105 2119 44-45 -1.7597342526368465 2119 46-47 -0.1764729814818935 2119 48-49 -0.6667603959290958 2119 50-51 0.46117843560283234 2119 52-53 -1.259288113166896 2119 54-55 -1.2623294516074068 2119 56-57 0.4183010605253301 2119 58-59 0.6651809422763151 2119 60-61 0.03435417491903081 2119 62-63 -0.639895468404319 2119 64-65 -0.3230691269521486 2119 66-67 -0.18892001390683077 2119 68-69 -1.2148265522745447 2119 70-71 -1.833720991686242 2119 72-73 -2.470722718591876 2119 74-75 0.20607747842865365 2119 76 0.9128150499850776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 17.0 13 88.0 14 277.0 15 507.0 16 714.0 17 801.0 18 867.0 19 877.0 20 819.0 21 768.0 22 810.0 23 921.0 24 1064.0 25 1258.0 26 1550.0 27 1906.0 28 2269.0 29 2951.0 30 3616.0 31 4652.0 32 5948.0 33 7863.0 34 10734.0 35 16577.0 36 28194.0 37 36173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.96291667301742 22.00155466475636 12.707098111539574 28.328430550686644 2 18.78946930564274 19.61307790626442 35.991468957745624 25.605983830347217 3 17.619476187955197 24.989600901507302 28.731763762734168 28.659159147803333 4 13.319165349447523 17.361578545336286 37.541123707675666 31.77813239754052 5 13.787313856136981 35.97483040015731 35.63525256574121 14.602603177964498 6 28.42395044735031 38.550781634057614 17.289730228477648 15.735537690114429 7 27.402948049885424 30.619483750935917 22.555833705179886 19.421734493998773 8 24.138009272214365 35.320632567707584 19.331735023407425 21.209623136670626 9 23.52767672795202 16.84578326009847 21.583234384335555 38.043305627613954 10-11 24.516158308312473 25.838167338511454 27.020261225354137 22.625413127821936 12-13 23.9712455472951 24.034018287287388 26.43450836843817 25.560227796979344 14-15 21.57264621132481 25.55379926336568 24.13309333474509 28.740461190564424 16-17 21.57226806228871 27.950507854155482 26.894715745369563 23.582508338186244 18-19 21.231555780764314 27.73420660550736 26.978286682347246 24.055950931381076 20-21 21.148741141858828 27.695257254789258 27.156394878349456 23.999606725002458 22-23 22.511968416992506 27.32315860326872 27.233915430749565 22.93095754898921 24-25 21.765124070698743 28.004961315353604 27.040303124267336 23.189611489680313 26-27 20.86361676864086 28.510546576616775 27.406351391210304 23.219485263532064 28-29 21.864955416228643 27.423746246870817 28.490504677703576 22.220793659196964 30-31 21.329118232077626 27.572358818057374 27.958827132949637 23.139695816915363 32-33 20.965717008387347 28.38991703410148 27.30954523796919 23.334820719541984 34-35 21.767392964915334 27.572358818057374 27.78071893694743 22.879529280079865 36-37 22.134197529930496 27.53945985191684 26.91967358175204 23.406669036400626 38-39 21.5544950575921 27.72059324020783 27.79206340803037 22.9328482941697 40-41 21.558654696989176 26.05711563041226 28.648949123828682 23.73528054876988 42-43 21.019036022477177 26.159972168230944 28.666722128525297 24.154269680766582 44-45 20.136814321260296 25.427119336272813 29.550834574922668 24.885231767544226 46-47 20.06156266307677 25.246364097017914 30.294275579891544 24.397797660013765 48-49 19.172156130174024 25.751193060208887 30.994607594745244 24.082043214871845 50-51 18.818964930458392 26.391777527359082 29.963395173305702 24.825862368876823 52-53 18.18178380463308 26.69580935238196 30.160410821112816 24.96199602187214 54-55 17.63649289457961 26.9877404082497 29.850328611512367 25.52543808565832 56-57 17.72460161999047 26.922698774040825 29.700203444181422 25.652496161787287 58-59 18.22262390053168 26.135014331848467 29.175332582077246 26.467029185542607 60-61 18.654091950719618 25.904343419828624 29.62835512732278 25.81320950212898 62-63 18.788334858534444 26.06278786595373 29.02861075607118 26.120266519440644 64-65 19.763581222631462 25.746277122739613 27.69336650960877 26.796775145020156 66-67 19.651649107946422 26.041233370896137 26.76387617887962 27.54324134227782 68-69 19.47883499844959 25.346195442547813 26.711691611898082 28.463277947104515 70-71 20.544458982174056 25.77426015141087 26.55854125227835 27.122739614136727 72-73 20.567904222412135 26.11724132715186 26.235601975450564 27.079252474985445 74-75 20.23778011389849 27.30538559857211 26.159215870158746 26.297618417370654 76 20.132654681863215 26.771439159601584 26.976395937166757 26.119510221368447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 2.0 12 4.0 13 5.5 14 4.0 15 2.0 16 6.5 17 10.0 18 11.0 19 16.5 20 26.5 21 32.0 22 37.0 23 63.0 24 137.5 25 191.0 26 267.5 27 377.0 28 559.0 29 708.0 30 821.5 31 1135.0 32 1619.0 33 1903.0 34 2139.0 35 2742.5 36 3550.5 37 3991.0 38 4639.0 39 5877.5 40 7175.5 41 8164.0 42 8445.0 43 8770.5 44 9712.0 45 10511.5 46 10695.0 47 10433.5 48 9908.0 49 9064.5 50 8485.0 51 7795.5 52 6475.0 53 5222.0 54 4600.0 55 4207.5 56 3234.5 57 2337.5 58 2021.0 59 1690.5 60 1166.0 61 794.5 62 617.0 63 498.0 64 328.5 65 220.5 66 130.5 67 98.0 68 85.5 69 62.5 70 47.0 71 42.0 72 34.0 73 24.0 74 15.5 75 9.0 76 14.0 77 15.0 78 11.0 79 11.0 80 7.0 81 4.0 82 2.5 83 0.0 84 2.0 85 3.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.5 94 1.5 95 1.5 96 1.0 97 1.0 98 2.0 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7600795625571951 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 132223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.038367001202516 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43059839605182 35.518782662622996 2 10.211289327575571 10.014899072022265 3 5.729487970388649 8.428942014626804 4 3.322023442319556 6.5162641900425795 5 1.9956816779765578 4.893248527109504 6 1.3355953115360888 3.9297247831315283 7 0.9762492288710672 3.351156757901424 8 0.6940160394818014 2.7226730599063704 9 0.525909932140654 2.3210787835701807 >10 2.756014805675509 21.63617524938929 >50 0.02313386798272671 0.6670548996770607 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006806682649765926 0.0 2 0.0 0.0 0.0 0.009831874938550781 0.0 3 0.0 0.0 0.0 0.012100769155139423 0.0 4 0.0 0.0 0.0 0.014369663371728066 0.0 5 0.0 0.0 0.0 0.014369663371728066 0.0 6 0.0 0.0 0.0 0.014369663371728066 0.0 7 0.0 0.0 0.0 0.014369663371728066 0.0 8 0.0 0.0 0.0 0.016638557588316706 0.0 9 0.0 0.0 0.0 0.020420047949297776 0.0 10 0.0 0.0 0.0 0.028739326743456133 0.0 11 0.0 0.0 0.0 0.031008220960044772 0.0 12 0.0 0.0 0.0 0.0325208171044372 0.0 13 0.0 0.0 0.0 0.035546009393222056 0.0 14 0.0 0.0 0.0 0.035546009393222056 0.0 15 0.0 0.0 0.0 0.039327499754203125 0.0 16 0.0 0.0 0.0 0.04159639397079177 0.0 17 0.0 0.0 0.0 0.04537788433177284 0.0 18 0.0 0.0 0.0 0.05294086505373498 0.0 19 0.0 0.0 0.0 0.058234951559108475 0.0 20 0.0 0.0 0.0 0.0597475477035009 0.0 21 0.0 0.0 0.0 0.06882312456985547 0.0 22 0.0 0.0 0.0 0.07411721107522896 0.0 23 0.0 0.0 0.0 0.07789870143621004 0.0 24 0.0 0.0 0.0 0.07941129758060246 0.0 25 0.0 0.0 0.0 0.0809238937249949 0.0 26 0.0 0.0 0.0 0.08848687444695703 0.0 27 0.0 0.0 0.0 0.12176398962359045 0.0 28 0.0 0.0 0.0 0.16109148937779358 0.0 29 0.0 0.0 0.0 0.19285600841003456 0.0 30 0.0 0.0 0.0 0.2336961043086301 0.0 31 0.0 0.0 0.0 0.2904184597233462 0.0 32 0.0 0.0 0.0 0.36453567079857513 0.0 33 0.0 0.0 0.0 0.4288210069352533 0.0 34 0.0 0.0 0.0 0.4968878334329126 0.0 35 0.0 0.0 0.0 0.5649546599305718 0.0 36 0.0 0.0 0.0 0.7978944661670058 0.0 37 0.0 0.0 0.0 1.2456229249071644 0.0 38 0.0 0.0 0.0 1.8688125363968446 0.0 39 0.0 0.0 0.0 2.6092283490769383 0.0 40 0.0 0.0 0.0 3.5803150737768767 7.562980721962139E-4 41 0.0 0.0 0.0 4.8123246333845096 7.562980721962139E-4 42 0.0 0.0 0.0 6.3748364505418875 7.562980721962139E-4 43 0.0 0.0 0.0 7.879869614212353 7.562980721962139E-4 44 0.0 0.0 0.0 9.14288739478003 7.562980721962139E-4 45 0.0 0.0 0.0 10.314393108611966 7.562980721962139E-4 46 0.0 0.0 0.0 11.567579014241092 7.562980721962139E-4 47 0.0 0.0 0.0 12.693706843741255 7.562980721962139E-4 48 0.0 0.0 0.0 13.711684048917359 7.562980721962139E-4 49 0.0 0.0 0.0 14.678989283256318 7.562980721962139E-4 50 0.0 0.0 0.0 15.547975768209767 7.562980721962139E-4 51 0.0 0.0 0.0 16.55763369459171 7.562980721962139E-4 52 0.0 0.0 0.0 17.918213926472703 7.562980721962139E-4 53 0.0 0.0 0.0 19.211483629928228 7.562980721962139E-4 54 0.0 0.0 0.0 20.578114246386786 7.562980721962139E-4 55 0.0 0.0 0.0 21.594578855418497 7.562980721962139E-4 56 0.0 0.0 0.0 22.487766878682226 7.562980721962139E-4 57 0.0 0.0 0.0 23.340871104119557 7.562980721962139E-4 58 0.0 0.0 0.0 24.263554752198935 7.562980721962139E-4 59 0.0 0.0 0.0 25.122709362213836 7.562980721962139E-4 60 0.0 0.0 0.0 25.887326713204207 7.562980721962139E-4 61 0.0 0.0 0.0 26.637574400822853 7.562980721962139E-4 62 0.0 0.0 0.0 27.404460646029815 7.562980721962139E-4 63 0.0 0.0 0.0 28.378572563018537 7.562980721962139E-4 64 0.0 0.0 0.0 29.327726643624786 7.562980721962139E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAACG 15 0.002221301 69.99395 4 ATAACGC 15 0.002221301 69.99395 5 AGCAACT 20 0.006940875 52.495464 58 TCGTTGT 20 0.006940875 52.495464 34 GCCTGGG 20 0.006940875 52.495464 54 AACGCTC 20 0.006940875 52.495464 7 CTGCAAT 20 0.006940875 52.495464 28 CATCGTT 20 0.006940875 52.495464 32 CATCGCC 20 0.006940875 52.495464 11 GCTCGGT 20 0.006940875 52.495464 12 TCGGTAG 20 0.006940875 52.495464 14 TGCCGAG 20 0.006940875 52.495464 57 CGGATCG 40 4.5750006E-5 43.74622 20 CGCGGTC 35 0.0012522748 39.996544 39 TCGTGCC 35 0.0012522748 39.996544 26 GTGCCAG 35 0.0012522748 39.996544 28 GGATCGG 45 9.162447E-5 38.88553 21 TACTGAC 55 6.658307E-6 38.178516 34 GACTATC 55 6.658307E-6 38.178516 38 ACCATTA 40 0.0024127455 34.996975 59 >>END_MODULE