FastQCFastQC Report
Wed 25 May 2016
SRR1780666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132223
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT13521.0225149936092814No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5190.39251869946983503RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1900.14369663371728067No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG150.0022205470.036
AGGCCAG150.0022205470.027
CCGCCAG150.0022205470.022
CCGTGCG452.6791895E-854.4444439
AAATACG200.00693850352.54
CGTGCGA407.4336094E-752.510
GGGTCCC200.00693850352.58
TCGGGCC200.00693850352.543
ACGGGCC200.00693850352.58
ATATCGT200.00693850352.537
GGGGTCC200.00693850352.57
CCCTTGA200.00693850352.512
CGTCCCA200.00693850352.531
CCACCGG200.00693850352.553
TTGAGTG451.6744125E-646.66666443
GTAATGG451.6744125E-646.66666458
CCCGTGC451.6744125E-646.6666648
ATTTGGC305.86044E-446.66666440
GATGTAT404.5726672E-543.7524
TATGCCG3400.042.20588343
Produced by FastQC (version 0.11.5)